BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135959.8 + phase: 0 /pseudo/partial
(1454 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP052828 107 1e-23
BG662087 102 4e-22
AV427422 63 4e-10
BP065719 52 9e-07
BP055130 49 6e-06
AV410603 47 3e-05
AU089582 47 3e-05
BF177840 43 3e-04
TC18698 37 0.017
AV771315 33 0.25
AU251673 31 1.2
TC15487 similar to UP|O23144 (O23144) Proton pump interactor (AT... 29 4.7
AV778124 29 4.7
TC15695 similar to UP|Q93WX6 (Q93WX6) NIFS-like protein CpNifsp ... 28 7.9
TC8188 similar to UP|Q9LFL3 (Q9LFL3) TOM (Target of myb1)-like p... 28 7.9
>BP052828
Length = 523
Score = 107 bits (268), Expect = 1e-23
Identities = 57/148 (38%), Positives = 85/148 (56%), Gaps = 3/148 (2%)
Frame = +2
Query: 458 PCVIGSEVVKKAMCDLGASVSLMPLSLYERLGIGELKSTRMTLQLADRSVKYPAGIIEDV 517
PC IG + M DLGA +++MP S+Y L G L+ T + +QLA+RS P G++EDV
Sbjct: 71 PCTIGDNKYENCMLDLGAGINVMPTSIYNILHPGPLQHTGLIVQLANRSNARPKGVVEDV 250
Query: 518 PVKVGEVYIPADFVVMEME---EDNQVPILLGRPFLATAGAIIDVKKGKLAFNVGKETVE 574
V+V E+ PADF +++ME + ++ PI+LGRPF+ TA IDV G ++ G +
Sbjct: 251 LVQVNELIFPADFYILDMEGETKSSRAPIILGRPFMKTAKTKIDVDNGTMSMEFGDIIAK 430
Query: 575 FELAKLMKGPSIKDSCCMIDIIDHCVKE 602
F + MK P S I ++ V +
Sbjct: 431 FNIDDAMKHPLEDHSVFHIGVVSELVDD 514
>BG662087
Length = 373
Score = 102 bits (254), Expect = 4e-22
Identities = 47/109 (43%), Positives = 70/109 (64%)
Frame = +1
Query: 808 WRMCIDYRKLNKATRKDHFPLPFIDQMLERLAKHSHFCYLDGYSGFFQIPIHPNDQEKTT 867
WRM +DY LNKA KD +PLP ID++++ + + +D YSG+ QI +HP+D++KT
Sbjct: 22 WRMWVDYTDLNKACPKDSYPLPSIDKLVDGASDNELLSLMDAYSGYHQIKMHPSDEDKTA 201
Query: 868 FTCPFGTFAYRRMPFGLCNAPATFQRCMMSIFSDFVEKIMEVFMDDFSV 916
F + Y+ +PFGL NA AT+Q M +F D V + MEV++D+ V
Sbjct: 202 FMTARVNYCYQTIPFGLKNAGATYQXLMDRVFXDXVGRNMEVYLDNMIV 348
>AV427422
Length = 417
Score = 62.8 bits (151), Expect = 4e-10
Identities = 34/113 (30%), Positives = 61/113 (53%), Gaps = 1/113 (0%)
Frame = +1
Query: 1343 PSSFGNQYILVAVDYVSKWVEAIA-SPTNDAQVVIKMFKKVIFPRFGVPRVVISDGGSHF 1401
P S G + +LV VD +SK+ + A+V+ +F + + GVP ++SD F
Sbjct: 13 PKSKGYEAVLVVVDRLSKFSHFVPLKHPYTAKVIADIFVREVVRLHGVPLSIVSDRDPLF 192
Query: 1402 ISKHFEKLLQKLGVRHKVATPYHPQTSGQVEVSNRQIKAILEKTVSTSRTDWS 1454
+S +++L + G + K++T YHP++ GQ EV NR ++ L ++ W+
Sbjct: 193 MSNFWKELFKMQGTKLKMSTAYHPESDGQTEVVNRCLETYLRCFIADQPKSWA 351
>BP065719
Length = 567
Score = 51.6 bits (122), Expect = 9e-07
Identities = 35/119 (29%), Positives = 54/119 (44%)
Frame = +3
Query: 2 QFSGSPSEDPNLHISNFWRLSVTCKDDQETVRLHLFPFSLKNKASNWFNSLKPGSITSWD 61
+FSG E HI+ + ++ E +++ FP SL A WF +L P S+ +W
Sbjct: 195 KFSGDSGESTVEHIARY-QIEAGDLAINENLKMKYFPSSLTKNAFTWFTTLAPRSVHTWA 371
Query: 62 QLRREFLCRFFPPSKTAQLRGKLYQFTQMNGESLFDVWERFKEILRRCPHHGLEKWLII 120
QL R F +FF + L + ES+ D RF+ + RC H E L++
Sbjct: 372 QLERIFHEQFFRGECKVSXK-DLASVKRKPAESIDDYLNRFRMLKSRCFTHVSEHELVV 545
>BP055130
Length = 567
Score = 48.9 bits (115), Expect = 6e-06
Identities = 31/108 (28%), Positives = 49/108 (44%), Gaps = 1/108 (0%)
Frame = +2
Query: 1347 GNQYILVAVDYVSKWVEAIASPTN-DAQVVIKMFKKVIFPRFGVPRVVISDGGSHFISKH 1405
G I+V VD +SK+ N + V + F I G+PR ++SD F S
Sbjct: 179 GFTVIIVVVDRLSKYGHFAPHRANYTSSQVAETFVSTIVKLHGMPRAIVSDRDKAFTSAF 358
Query: 1406 FEKLLQKLGVRHKVATPYHPQTSGQVEVSNRQIKAILEKTVSTSRTDW 1453
++ + G +++ YHPQT GQ E N+ ++ L V + W
Sbjct: 359 WKHFFKLHGTTLNMSSSYHPQTDGQTEALNKCLELYLRCFVHETPRLW 502
>AV410603
Length = 162
Score = 46.6 bits (109), Expect = 3e-05
Identities = 22/54 (40%), Positives = 31/54 (56%)
Frame = +1
Query: 820 ATRKDHFPLPFIDQMLERLAKHSHFCYLDGYSGFFQIPIHPNDQEKTTFTCPFG 873
+T KD FP+P +D++L+ L F LD SG+ QI + P D+ KT F G
Sbjct: 1 STVKDSFPMPTVDELLDELRGSQFFSKLDLRSGYHQILVKPEDRHKTVFRTHHG 162
>AU089582
Length = 383
Score = 46.6 bits (109), Expect = 3e-05
Identities = 26/74 (35%), Positives = 39/74 (52%)
Frame = +2
Query: 1377 KMFKKVIFPRFGVPRVVISDGGSHFISKHFEKLLQKLGVRHKVATPYHPQTSGQVEVSNR 1436
K++ I GVP +ISD G+ F S + LG R K++T +HPQT GQ E + +
Sbjct: 26 KIYLDEIVSLHGVPVSIISDRGAQFTSHFWRSFQTALGTRLKMSTAFHPQTDGQSERTIQ 205
Query: 1437 QIKAILEKTVSTSR 1450
++ +L V R
Sbjct: 206 ILEDMLRACVXDLR 247
>BF177840
Length = 410
Score = 43.1 bits (100), Expect = 3e-04
Identities = 20/61 (32%), Positives = 32/61 (51%)
Frame = +2
Query: 1393 VISDGGSHFISKHFEKLLQKLGVRHKVATPYHPQTSGQVEVSNRQIKAILEKTVSTSRTD 1452
++SD + FIS + L K+G + +T HPQT GQ EV N+ + +L + +
Sbjct: 17 IVSDRDTKFISHFWRTLWGKVGTKLLYSTTCHPQTDGQTEVVNKTLSTLLRSVLERNLKM 196
Query: 1453 W 1453
W
Sbjct: 197 W 199
>TC18698
Length = 808
Score = 37.4 bits (85), Expect = 0.017
Identities = 21/57 (36%), Positives = 31/57 (53%)
Frame = -2
Query: 863 QEKTTFTCPFGTFAYRRMPFGLCNAPATFQRCMMSIFSDFVEKIMEVFMDDFSVHGS 919
++KTT + Y+ MP GL N T+QR M IF + K +EV+++D V S
Sbjct: 807 KKKTTLKINRVNYYYQVMPLGLKNI*TTYQRLMDKIFHKQI*KNVEVYVEDMIVKSS 637
>AV771315
Length = 511
Score = 33.5 bits (75), Expect = 0.25
Identities = 20/59 (33%), Positives = 27/59 (44%)
Frame = +1
Query: 312 NKMNAVTLRSGKPLEDPIRRTETDHSEKEIREPPSRETRAEKEEPRVEGNTTPPFKPKI 370
N + A LR G P T+ SEK+ ++ P ET +VE + PP K KI
Sbjct: 118 NNLKATELRLGLP--------GTEESEKKTKKRPLNETSEGSNAQQVESGSAPPSKAKI 270
>AU251673
Length = 413
Score = 31.2 bits (69), Expect = 1.2
Identities = 26/92 (28%), Positives = 40/92 (43%), Gaps = 5/92 (5%)
Frame = +2
Query: 30 ETVRLHLFPFSLKNKASNWFNSL---KP-GSIT-SWDQLRREFLCRFFPPSKTAQLRGKL 84
+T + L F L+ A +W+N L KP GS +W EF+ RF P S
Sbjct: 11 DTRAVELASFQLEGVARDWYNVLTRAKPVGSPPWTWADFSAEFMNRFLPQSVRDGFVRDF 190
Query: 85 YQFTQMNGESLFDVWERFKEILRRCPHHGLEK 116
+ Q G ++ + F + R P+ LE+
Sbjct: 191 ERLEQAEGMTVSEYSAHFTHLSRYVPYPLLEE 286
>TC15487 similar to UP|O23144 (O23144) Proton pump interactor
(AT4g27500/F27G19_100), partial (16%)
Length = 1153
Score = 29.3 bits (64), Expect = 4.7
Identities = 35/138 (25%), Positives = 58/138 (41%), Gaps = 9/138 (6%)
Frame = +2
Query: 323 KPLEDPIRRTETDHSEKEIREPPSRETRAEKEEPRVEGNTTPPFKPKIPFPQRFAKSKLD 382
KPL + + ETD K I + P KEEP+ T P + + R K+K D
Sbjct: 191 KPLLEEPKPAETDAFSKAIPKQP-------KEEPKPSPQETLPTQKESKNKVRDLKAKPD 349
Query: 383 EQFKKFMEMMNKIYIDVPFTEVLTQMPTYAKFLKEILSKKRKIEESETV-----NLTEEC 437
KK +E ++ +VP E + P + + ++ +I +++ L E+
Sbjct: 350 ---KKDLEEADEYEFEVPRKETPPKEPEIDPAKLKEMKREEEIAKAKQAWERKKKLAEKA 520
Query: 438 SA----IIQNKLPPKLKD 451
+A Q + KLKD
Sbjct: 521 AAKAALKAQKEAEKKLKD 574
>AV778124
Length = 515
Score = 29.3 bits (64), Expect = 4.7
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = -3
Query: 1220 NYQQKKKFFN-DLKHYYWDEPYLFRRGSDGIFRRC 1253
N +Q KF N + HYYWDE +L + S + C
Sbjct: 117 NEKQLFKFININTDHYYWDENHLMSKLSSSLMLIC 13
>TC15695 similar to UP|Q93WX6 (Q93WX6) NIFS-like protein CpNifsp precursor
(At1g08490/T27G7_14) (Cysteine desulfurase) , partial
(27%)
Length = 602
Score = 28.5 bits (62), Expect = 7.9
Identities = 18/77 (23%), Positives = 37/77 (47%)
Frame = +3
Query: 154 LIENMALNLFQWTEEKETVDPSPSEKEAGINETSSIDYLSTKLNALSQRLDRMSTPTFSP 213
++++ N +W ++ T+ PSPS I E+ + L++ L + L +S P+ +
Sbjct: 177 ILQHFLTNSMEWLSDQVTIVPSPS---IAI*ESMQVHVLASISITLRRMLTTLSRPSMTQ 347
Query: 214 PCETCGLSSHSDTDCNL 230
+ SS++ C L
Sbjct: 348 *ASSIHSSSNNVFLCKL 398
>TC8188 similar to UP|Q9LFL3 (Q9LFL3) TOM (Target of myb1)-like protein
(AT5g16880/F2K13_30), partial (77%)
Length = 1574
Score = 28.5 bits (62), Expect = 7.9
Identities = 13/24 (54%), Positives = 18/24 (74%)
Frame = +3
Query: 180 EAGINETSSIDYLSTKLNALSQRL 203
E G+NET + + L K+NALS+RL
Sbjct: 81 EVGLNETMTDNNLMEKVNALSERL 152
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.137 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,576,136
Number of Sequences: 28460
Number of extensions: 365776
Number of successful extensions: 1782
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 1768
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1779
length of query: 1454
length of database: 4,897,600
effective HSP length: 102
effective length of query: 1352
effective length of database: 1,994,680
effective search space: 2696807360
effective search space used: 2696807360
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Medicago: description of AC135959.8