BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135797.5 - phase: 0
(144 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8399 117 8e-28
TC10698 48 6e-07
AU251952 32 0.042
TC10779 30 0.21
TC11169 similar to UP|Q9SL72 (Q9SL72) At2g20090 protein, partial... 27 1.0
CB829198 27 1.0
AV412298 27 1.4
TC14386 similar to UP|Q9FJD4 (Q9FJD4) Importin beta, partial (12%) 27 1.8
TC10166 weakly similar to UP|Q8W0T8 (Q8W0T8) Gb protein, partial... 27 1.8
TC10224 26 2.3
TC8008 similar to UP|RERA_ARATH (O48670) RER1A protein (AtRER1A)... 26 2.3
BI418174 26 2.3
AW720177 26 3.0
TC17739 similar to GB|AAP21350.1|30102864|BT006542 At1g78995 {Ar... 26 3.0
TC14233 homologue to UP|Q41027 (Q41027) PsHSC71.0, partial (67%) 26 3.0
TC10380 similar to UP|Q8S8U2 (Q8S8U2) Ycf1 protein, partial (40%) 25 4.0
AV780606 25 4.0
BP037000 25 4.0
TC8655 similar to UP|Q9M621 (Q9M621) ReMembR-H2 protein JR702 (F... 25 4.0
TC16865 similar to UP|RS24_ARATH (Q9SS17) 40S ribosomal protein ... 25 5.2
>TC8399
Length = 600
Score = 117 bits (293), Expect = 8e-28
Identities = 67/101 (66%), Positives = 76/101 (74%), Gaps = 3/101 (2%)
Frame = +3
Query: 47 HTVKNKKQPKSRKKKN-EKKNAKKGNKGNST-RGGNGKDKKQSRNVCGQSDLCSKSWDSQ 104
HTVKNKKQ KSRKKK EKKN KKG KGNS+ GG+GKDKKQ+R G SDL +S DS
Sbjct: 3 HTVKNKKQQKSRKKKKYEKKNGKKGQKGNSSSHGGSGKDKKQARKQGGHSDLSFESLDSH 182
Query: 105 V-IEGSSEFDSLDVCSQDSNCGSSFLKKSVTEVHYSVAEAQ 144
+ G+SE D+LDV S DS CG+SFLK SVT+VH VAEAQ
Sbjct: 183 DNVTGTSELDALDVRSHDSFCGTSFLKNSVTKVHSLVAEAQ 305
>TC10698
Length = 538
Score = 48.1 bits (113), Expect = 6e-07
Identities = 38/89 (42%), Positives = 50/89 (55%)
Frame = +1
Query: 47 HTVKNKKQPKSRKKKNEKKNAKKGNKGNSTRGGNGKDKKQSRNVCGQSDLCSKSWDSQVI 106
HTVK+K + KSR KK EKK++KKG++ ++GGN KD + L S + I
Sbjct: 1 HTVKSKNRHKSRIKKGEKKSSKKGHQHPYSKGGNTKDITPVKKPYDHWWLES---PDKGI 171
Query: 107 EGSSEFDSLDVCSQDSNCGSSFLKKSVTE 135
E S+ FD S DS G S+L K VTE
Sbjct: 172 ETSTGFDLDADHSYDSFHGISYL-KPVTE 255
>AU251952
Length = 323
Score = 32.0 bits (71), Expect = 0.042
Identities = 19/51 (37%), Positives = 26/51 (50%), Gaps = 4/51 (7%)
Frame = -1
Query: 43 TLVSHTVKNKKQPKSRKKKNEKKNAK----KGNKGNSTRGGNGKDKKQSRN 89
T+ S T +NKK K+ KKK +KK + K + S G+DK S N
Sbjct: 221 TMTSLTHENKKTTKNHKKKKKKKKLQNRLTKQKEEGSVLDQKGRDKSSSTN 69
>TC10779
Length = 669
Score = 29.6 bits (65), Expect = 0.21
Identities = 16/48 (33%), Positives = 24/48 (49%)
Frame = +3
Query: 41 VPTLVSHTVKNKKQPKSRKKKNEKKNAKKGNKGNSTRGGNGKDKKQSR 88
+PT +VK+ K K +EKK K G+K RG + + K S+
Sbjct: 87 LPTGGLISVKSSKAEKKGSHSSEKKRDKDGHKSERKRGKDDSNHKSSK 230
>TC11169 similar to UP|Q9SL72 (Q9SL72) At2g20090 protein, partial (14%)
Length = 650
Score = 27.3 bits (59), Expect = 1.0
Identities = 13/45 (28%), Positives = 23/45 (50%)
Frame = +3
Query: 66 NAKKGNKGNSTRGGNGKDKKQSRNVCGQSDLCSKSWDSQVIEGSS 110
+ K+ N GNS G+G ++ S C C S+ S +++ S+
Sbjct: 357 DVKQENSGNSYVYGHGNEELHSAKSCWSPKSCVTSFSSDMLDFSN 491
>CB829198
Length = 562
Score = 27.3 bits (59), Expect = 1.0
Identities = 20/71 (28%), Positives = 30/71 (42%)
Frame = +3
Query: 50 KNKKQPKSRKKKNEKKNAKKGNKGNSTRGGNGKDKKQSRNVCGQSDLCSKSWDSQVIEGS 109
K K+ ++KKKN++++ K N+ +GG G LCS S Q G
Sbjct: 354 KPKRAKAAKKKKNKERSNKSDNEVVKKKGG------------GFCKLCSLSPQLQEFTGV 497
Query: 110 SEFDSLDVCSQ 120
E +V Q
Sbjct: 498 PEMARTEVVKQ 530
>AV412298
Length = 342
Score = 26.9 bits (58), Expect = 1.4
Identities = 13/38 (34%), Positives = 22/38 (57%), Gaps = 1/38 (2%)
Frame = -2
Query: 70 GNKGNSTRGGNGKDKKQSRNVCGQSDLC-SKSWDSQVI 106
GN+ RGG K++ + R++ G + LC K + Q+I
Sbjct: 281 GNRETQNRGGERKNRGRQRDIVGIAILCVQKRIEMQII 168
>TC14386 similar to UP|Q9FJD4 (Q9FJD4) Importin beta, partial (12%)
Length = 887
Score = 26.6 bits (57), Expect = 1.8
Identities = 21/84 (25%), Positives = 34/84 (40%), Gaps = 2/84 (2%)
Frame = -1
Query: 45 VSHTVKNKKQPKSRKKKNEKKNAKKGNKGNSTRGGNGKDKKQSRNVCGQSDLCSKSWDSQ 104
VS + + + S + K +K K +S D ++ RN C Q+ SW
Sbjct: 560 VSRKIGHHPRTTSTAMQKNKLEKEKYEKDDSMLVT*NADSQKDRNTCAQNW**PDSW--H 387
Query: 105 VIEGSSEFDSLDVCSQDS--NCGS 126
+E + +DVC + NC S
Sbjct: 386 AVETPKQQMQIDVCKELDP*NCNS 315
>TC10166 weakly similar to UP|Q8W0T8 (Q8W0T8) Gb protein, partial (5%)
Length = 980
Score = 26.6 bits (57), Expect = 1.8
Identities = 12/38 (31%), Positives = 19/38 (49%), Gaps = 3/38 (7%)
Frame = +3
Query: 47 HTVKNKKQPKSRK---KKNEKKNAKKGNKGNSTRGGNG 81
H VK KK + +++ + + GN+G RGG G
Sbjct: 441 HEVKRKKDKRKENLNSRESVEPRGRPGNQGRGPRGGRG 554
>TC10224
Length = 595
Score = 26.2 bits (56), Expect = 2.3
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = -1
Query: 61 KNEKKNAKKGNKGNSTRGGNGKDKKQSRNVCGQSDL 96
K K+ +KGN+ +S N DK+ ++C DL
Sbjct: 505 KGNKRTTQKGNQNSS----NSSDKRTPVDICNNYDL 410
>TC8008 similar to UP|RERA_ARATH (O48670) RER1A protein (AtRER1A), partial
(83%)
Length = 1154
Score = 26.2 bits (56), Expect = 2.3
Identities = 13/44 (29%), Positives = 21/44 (47%)
Frame = -2
Query: 31 VKWAADVYDPVPTLVSHTVKNKKQPKSRKKKNEKKNAKKGNKGN 74
V+W V+D V L H + P + + E +N K G +G+
Sbjct: 715 VEWDILVFDHVCNLAPHGKDKENSPVTE*YRPEDRNIKYGKEGH 584
>BI418174
Length = 602
Score = 26.2 bits (56), Expect = 2.3
Identities = 16/33 (48%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = -3
Query: 40 PVPTLVSHTVKNKKQPKSRKKK-NEKKNAKKGN 71
P LV T +NK K +KKK N+KKN K G+
Sbjct: 102 PQLKLVPGTKRNKTGLKLKKKKMNKKKNLKGGH 4
>AW720177
Length = 505
Score = 25.8 bits (55), Expect = 3.0
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +2
Query: 45 VSHTVKNKKQPKSRKKKNEKKNAKKGN 71
+SH+ K KK+ K +KKK KK+ G+
Sbjct: 20 LSHSQKKKKKKKKKKKKLLKKHEGIGS 100
>TC17739 similar to GB|AAP21350.1|30102864|BT006542 At1g78995 {Arabidopsis
thaliana;}, partial (36%)
Length = 425
Score = 25.8 bits (55), Expect = 3.0
Identities = 16/52 (30%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Frame = +2
Query: 53 KQPKSRKKKNEKKNAKKGNKGNST-RGGNGKDKKQSRNVCGQSDLCSKSWDS 103
K+PK +K K+ K + N+ RGG G+ R++ SD+C+ D+
Sbjct: 8 KKPKIQKPKSRKAESCTVKCVNTCIRGGEGEGPLNIRSLVECSDICNLIGDA 163
>TC14233 homologue to UP|Q41027 (Q41027) PsHSC71.0, partial (67%)
Length = 1570
Score = 25.8 bits (55), Expect = 3.0
Identities = 20/69 (28%), Positives = 29/69 (41%)
Frame = +1
Query: 39 DPVPTLVSHTVKNKKQPKSRKKKNEKKNAKKGNKGNSTRGGNGKDKKQSRNVCGQSDLCS 98
D + +V K K + + KKK E KNA + N KD+K + G+ D
Sbjct: 919 DDIEKMVQEAEKYKSEDEEHKKKVEAKNALENYAYNMR--NTVKDEK----IAGKLDSAD 1080
Query: 99 KSWDSQVIE 107
K+ IE
Sbjct: 1081KTKIEDAIE 1107
>TC10380 similar to UP|Q8S8U2 (Q8S8U2) Ycf1 protein, partial (40%)
Length = 5664
Score = 25.4 bits (54), Expect = 4.0
Identities = 13/25 (52%), Positives = 16/25 (64%), Gaps = 1/25 (4%)
Frame = +3
Query: 49 VKNKKQPKSRKKKNEK-KNAKKGNK 72
++ KKQ K RKKK +K K KK K
Sbjct: 2223 IRTKKQKKVRKKKRKK*KKTKKRKK 2297
>AV780606
Length = 585
Score = 25.4 bits (54), Expect = 4.0
Identities = 15/58 (25%), Positives = 27/58 (45%), Gaps = 2/58 (3%)
Frame = -1
Query: 45 VSHTVKNKKQPKSRKKKNEKKNAKK--GNKGNSTRGGNGKDKKQSRNVCGQSDLCSKS 100
V + V+ + + + K + ++KK G G+ T +G+D Q+ C SKS
Sbjct: 579 VRYGVRQELALRMHRHKGQSTSSKKQDGANGSGTDQESGQDDSQAETSCTHCGTSSKS 406
>BP037000
Length = 512
Score = 25.4 bits (54), Expect = 4.0
Identities = 13/41 (31%), Positives = 19/41 (45%), Gaps = 9/41 (21%)
Frame = -1
Query: 96 LCSKSWDSQV---------IEGSSEFDSLDVCSQDSNCGSS 127
L S +W QV ++ + L +CSQDS+C S
Sbjct: 287 LFSSTWTDQVLGLKEGVTIVKDGCALEGLCICSQDSHCNPS 165
>TC8655 similar to UP|Q9M621 (Q9M621) ReMembR-H2 protein JR702 (Fragment),
partial (7%)
Length = 790
Score = 25.4 bits (54), Expect = 4.0
Identities = 15/29 (51%), Positives = 16/29 (54%)
Frame = -3
Query: 54 QPKSRKKKNEKKNAKKGNKGNSTRGGNGK 82
Q KS+K NEK AKK K S GN K
Sbjct: 479 QLKSQKCYNEKPGAKKEVKCRSRHIGNAK 393
>TC16865 similar to UP|RS24_ARATH (Q9SS17) 40S ribosomal protein S24,
complete
Length = 634
Score = 25.0 bits (53), Expect = 5.2
Identities = 17/55 (30%), Positives = 27/55 (48%), Gaps = 7/55 (12%)
Frame = +2
Query: 35 ADVYDPVPTLVSHTVKNKKQPKSRKKKNEKKN-------AKKGNKGNSTRGGNGK 82
A Y+P L+ + + K + KSRK+ E+KN KK ++ +GG K
Sbjct: 317 AKKYEPKYRLIRNGLDTKVE-KSRKQLKERKNRAKKIRGVKKTKASDAAKGGKKK 478
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.305 0.123 0.348
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,393,877
Number of Sequences: 28460
Number of extensions: 29297
Number of successful extensions: 195
Number of sequences better than 10.0: 51
Number of HSP's better than 10.0 without gapping: 185
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 189
length of query: 144
length of database: 4,897,600
effective HSP length: 82
effective length of query: 62
effective length of database: 2,563,880
effective search space: 158960560
effective search space used: 158960560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (21.9 bits)
S2: 51 (24.3 bits)
Medicago: description of AC135797.5