BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135795.7 - phase: 0
(591 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19911 weakly similar to PIR|A44805|A44805 eggshell protein pre... 42 3e-04
TC14184 similar to UP|Q93WZ6 (Q93WZ6) Abscisic stress ripening-l... 40 8e-04
TC15380 similar to UP|Q8H5P5 (Q8H5P5) OJ1003_C06.27 protein, par... 36 0.015
TC19260 34 0.058
TC17115 similar to UP|O02402 (O02402) Insoluble protein, partial... 33 0.17
TC19608 weakly similar to UP|Q7T2U0 (Q7T2U0) Nicotinic acetylcho... 32 0.38
TC16842 32 0.38
CB829398 31 0.65
TC15523 similar to UP|GRPA_MAIZE (P10979) Glycine-rich RNA-bindi... 31 0.65
BP043250 30 1.1
TC10343 similar to UP|Q9SZZ1 (Q9SZZ1) Fibrillarin-like protein (... 30 1.4
BP033767 30 1.4
TC13330 similar to PIR|T08400|T08400 late embryonic abundant pro... 29 1.9
TC8682 similar to UP|HPPD_SOLSC (Q9ARF9) 4-hydroxyphenylpyruvate... 29 2.5
AV408190 28 3.2
BP043617 28 3.2
AV418346 28 4.2
TC8843 28 4.2
TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding p... 28 4.2
BU494249 28 5.5
>TC19911 weakly similar to PIR|A44805|A44805 eggshell protein precursor -
fluke (Schistosoma haematobium) {Schistosoma
haematobium;} , partial (24%)
Length = 350
Score = 42.0 bits (97), Expect = 3e-04
Identities = 33/112 (29%), Positives = 48/112 (42%), Gaps = 1/112 (0%)
Frame = +1
Query: 255 GSSFNHTDFYSMVNNGRNVSPRQSNFGSLGFDEESGVAKANGNGGNGY-PAPHSAGIFSP 313
G+ H+D + GR G+D+++ ++ G G GY HSAG +
Sbjct: 58 GNDHRHSD---NEHGGRRSETTGHGLSGGGYDDDN---RSGGRTGGGYGDGGHSAGGYGD 219
Query: 314 VANKKKGHGGGAGDGGKDLHMFVWSSSASPVSEGGIHVFRGGGDYGNDQLNG 365
+ G GGG GDGG SS + +GG R G YG+ L+G
Sbjct: 220 HGGRSGGGGGGYGDGG-------LSSGSGGYGDGG----RSGVGYGDGXLSG 342
>TC14184 similar to UP|Q93WZ6 (Q93WZ6) Abscisic stress ripening-like
protein, partial (57%)
Length = 1141
Score = 40.4 bits (93), Expect = 8e-04
Identities = 38/163 (23%), Positives = 65/163 (39%), Gaps = 13/163 (7%)
Frame = +3
Query: 225 NSGVSLTPRPSNLTNAEIYSLQSSRNPTPRGSSFNHTDFYSMVNNGRNVSP--RQSNFGS 282
N +++T N + ++ PT + +N T + + +G + ++S++ +
Sbjct: 60 NLPLAITMAEENRDRGLFHHHKNEDRPTDTETGYNKTSYSTDEPSGGDYDSGYKKSSYET 239
Query: 283 LGFDEESGVAKANG-------NGGNGYPAPHSAGIFSPVANKKKGHG-GGAGDG---GKD 331
G E+G K + +GG GY + ++ +S G+G GGAG G G
Sbjct: 240 SGGGYETGYNKTSSYSTDEPNSGGGGYDSGYNKTAYSTDEPSGGGYGTGGAGGGYSTGGG 419
Query: 332 LHMFVWSSSASPVSEGGIHVFRGGGDYGNDQLNGVAHQKDYEE 374
GG GGG YGND + DY+E
Sbjct: 420 YGTGGGGGGEYSTGGGGGGYSTGGGGYGNDDSGNRRDEVDYKE 548
>TC15380 similar to UP|Q8H5P5 (Q8H5P5) OJ1003_C06.27 protein, partial (28%)
Length = 1306
Score = 36.2 bits (82), Expect = 0.015
Identities = 19/42 (45%), Positives = 22/42 (52%)
Frame = -2
Query: 316 NKKKGHGGGAGDGGKDLHMFVWSSSASPVSEGGIHVFRGGGD 357
N+ KG GGG G GGK+ A P GG+H GGGD
Sbjct: 339 NEGKGRGGGGGGGGKE---------AEPEVAGGVHGDVGGGD 241
>TC19260
Length = 498
Score = 34.3 bits (77), Expect = 0.058
Identities = 17/46 (36%), Positives = 26/46 (55%)
Frame = +1
Query: 434 ILIMVWRKLIRNPNTYSSLIGLTWSLVSFRWNVVMPAIVAKSIAIL 479
I++MV + + N + Y LIG W+L++ W VV I KS+ L
Sbjct: 301 IMVMVVYEKL*NSSCYMLLIGFVWNLMA*VWYVVAETIYRKSVVFL 438
>TC17115 similar to UP|O02402 (O02402) Insoluble protein, partial (3%)
Length = 667
Score = 32.7 bits (73), Expect = 0.17
Identities = 44/157 (28%), Positives = 56/157 (35%), Gaps = 4/157 (2%)
Frame = +3
Query: 179 SDVLSLDGKEPLQTEA---EVGEDGKLHVKVRKSTSSRSEIFSRRSHGVNSGVSLTPRPS 235
S+V SL T A G G V S+ I++ S G NSGV P+
Sbjct: 3 SNVGSLPASHASNTAAFWMVAGNGGNQGVSGGGGASNGDPIWAIPSVG-NSGVYRGAVPA 179
Query: 236 NLTNAEIYSLQSSRNPTPRGSSFNHTDFYSMVNNGRNVSPRQSNFGSLGFDEESGVAKAN 295
+ S P GS S++ NG S + G S +
Sbjct: 180 AAGGIHFMNFASPMMPFMPGSQLGS----SLMGNGGGGSGGSAFMGESNLGMLSALNGYR 347
Query: 296 GNGGNGYP-APHSAGIFSPVANKKKGHGGGAGDGGKD 331
N NG P +P SAG + HGGG GD G D
Sbjct: 348 QNPANGLPESPTSAG---------QHHGGGGGDDGHD 431
>TC19608 weakly similar to UP|Q7T2U0 (Q7T2U0) Nicotinic acetylcholine
receptor alpha 7b subunit (Fragment), partial (3%)
Length = 530
Score = 31.6 bits (70), Expect = 0.38
Identities = 25/83 (30%), Positives = 35/83 (42%), Gaps = 10/83 (12%)
Frame = -3
Query: 287 EESGVAKANGNGGNGYPAPHSAGIFSPVANKKKGHGGGAG----------DGGKDLHMFV 336
+ SG A + N Y P +F+P +++K G GA DGG +
Sbjct: 477 QRSGGASKHPQN*NNYKPP----LFNPELSQRKTIGTGASGLG*RSTHGCDGGDPRRLVT 310
Query: 337 WSSSASPVSEGGIHVFRGGGDYG 359
S+S VS GG +GGG G
Sbjct: 309 RSASPHTVSSGGSENGQGGGRGG 241
>TC16842
Length = 563
Score = 31.6 bits (70), Expect = 0.38
Identities = 19/63 (30%), Positives = 30/63 (47%)
Frame = +1
Query: 399 SKLGSSSTTELHPKAGSQGEAKPTNMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLTWS 458
S SST LHP++ ++ +VMT +L+M+ + P +SSLI W
Sbjct: 211 SPTSPSSTPPLHPRSLLPTSSETHRWWSTTVMTTTLLMMMSSAMCITPRVFSSLITRRW* 390
Query: 459 LVS 461
+S
Sbjct: 391 RIS 399
>CB829398
Length = 366
Score = 30.8 bits (68), Expect = 0.65
Identities = 28/96 (29%), Positives = 34/96 (35%), Gaps = 1/96 (1%)
Frame = -3
Query: 235 SNLTNAEIYSLQSSRNPTPRGSSFNHTDFYSMVNNGRNVSPRQSNFGSLGFDEE-SGVAK 293
S+L+ IYSL F H + M N G P S S SG
Sbjct: 289 SSLSKQGIYSLMV----------FGHNPNFLMANLGAAEEPSLSTCVSFTQSSTISGSKS 140
Query: 294 ANGNGGNGYPAPHSAGIFSPVANKKKGHGGGAGDGG 329
N + P+ + FS G GGG GDGG
Sbjct: 139 PTSNSSDIVPSSFTIPNFSTGGGGGGGDGGGGGDGG 32
>TC15523 similar to UP|GRPA_MAIZE (P10979) Glycine-rich RNA-binding,
abscisic acid-inducible protein, complete
Length = 972
Score = 30.8 bits (68), Expect = 0.65
Identities = 26/100 (26%), Positives = 37/100 (37%), Gaps = 3/100 (3%)
Frame = +2
Query: 269 NGRNVSPRQSNFGSLGFDEESGVAKANGNGGNGYPAPHSAGIFSPVANKKKGHGGGAGDG 328
NG+++ R ++ +E + G GG GY G + + GGG G G
Sbjct: 542 NGQDIDGR-----NVTVNEAQARGRGGGGGGGGYGGGRREGGYGGGGGSRYSGGGGGGYG 706
Query: 329 GKDLHMFVWSSSASPVSEGGIHVFR---GGGDYGNDQLNG 365
G S GG + R GGG YG + G
Sbjct: 707 G---------------SGGGGYGGRREGGGGGYGGSREGG 781
>BP043250
Length = 455
Score = 30.0 bits (66), Expect = 1.1
Identities = 25/73 (34%), Positives = 34/73 (46%), Gaps = 1/73 (1%)
Frame = +2
Query: 174 SFKVDSDVLSLDG-KEPLQTEAEVGEDGKLHVKVRKSTSSRSEIFSRRSHGVNSGVSLTP 232
+F S LS D K+ ++ V G+LH +R S + R + + GV LTP
Sbjct: 212 TFAASSASLSEDQPKDSSVSDMLVDSFGRLHTYLRISLTERCNLRCQYCMPAE-GVELTP 388
Query: 233 RPSNLTNAEIYSL 245
P LT AEI L
Sbjct: 389 TPQILTKAEILRL 427
>TC10343 similar to UP|Q9SZZ1 (Q9SZZ1) Fibrillarin-like protein (Fibrillarin
2) (AtFib2), partial (85%)
Length = 1116
Score = 29.6 bits (65), Expect = 1.4
Identities = 26/107 (24%), Positives = 38/107 (35%)
Frame = +1
Query: 265 SMVNNGRNVSPRQSNFGSLGFDEESGVAKANGNGGNGYPAPHSAGIFSPVANKKKGHGGG 324
S+ + V P + G GF + G+GG G +P + G GGG
Sbjct: 10 SLFKLSKMVPPPRGRGGGGGFRGRGDRGRGRGDGGG-----RGGGRGTPFKARGGGRGGG 174
Query: 325 AGDGGKDLHMFVWSSSASPVSEGGIHVFRGGGDYGNDQLNGVAHQKD 371
G GG+ GG RGGG G ++ H+ +
Sbjct: 175 GGRGGR----------------GGGRGGRGGGMKGGSRVVVEPHRHE 267
>BP033767
Length = 541
Score = 29.6 bits (65), Expect = 1.4
Identities = 18/43 (41%), Positives = 20/43 (45%), Gaps = 2/43 (4%)
Frame = -1
Query: 317 KKKGHGGGAGDG--GKDLHMFVWSSSASPVSEGGIHVFRGGGD 357
K++G GGG GDG GK W V E G F G GD
Sbjct: 340 KEEGEGGGGGDGYVGKKGVEAFWVVVVVGVEENGYFGFGGEGD 212
>TC13330 similar to PIR|T08400|T08400 late embryonic abundant protein EMB8
homolog F18B3.70 - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (21%)
Length = 434
Score = 29.3 bits (64), Expect = 1.9
Identities = 26/96 (27%), Positives = 40/96 (41%), Gaps = 21/96 (21%)
Frame = -2
Query: 299 GNGYPAPHSAGIFSPVANKKKGHGGGAGDGGKD-LHMFVWSSS----------------- 340
GN + +A + S + GGGA +GG+D L++ V S
Sbjct: 325 GNPVKSDSAAVLGSEALTAELDVGGGAEEGGEDGLYVAVPSKEGERVVGTSQEGESREET 146
Query: 341 ---ASPVSEGGIHVFRGGGDYGNDQLNGVAHQKDYE 373
AS EGG+ V+ GGG +G + G ++ E
Sbjct: 145 VTCASEDFEGGVWVWDGGGGHGYGEERGRRRRRQVE 38
>TC8682 similar to UP|HPPD_SOLSC (Q9ARF9) 4-hydroxyphenylpyruvate
dioxygenase (4HPPD) (HPD) (HPPDase) , partial (29%)
Length = 576
Score = 28.9 bits (63), Expect = 2.5
Identities = 24/80 (30%), Positives = 29/80 (36%), Gaps = 5/80 (6%)
Frame = -2
Query: 281 GSLGFDEESGVAKANGNGGNGYPAPHSAGIFSPVANKKKGHGGGAGDGGKDLHMFVWSSS 340
G G D GV +G+G +G G G GGG GD G+ VW
Sbjct: 563 GGEGLD---GVGDFDGDGADGEAVGGGEGEAGGGGESGDGGGGGEGDFGR-----VWGGE 408
Query: 341 -----ASPVSEGGIHVFRGG 355
A GG+ V GG
Sbjct: 407 EEAEVAGAEEVGGVRVVSGG 348
>AV408190
Length = 382
Score = 28.5 bits (62), Expect = 3.2
Identities = 24/82 (29%), Positives = 39/82 (47%), Gaps = 1/82 (1%)
Frame = -1
Query: 281 GSLGFDEESGVAKANGNGGNGYPAPHSAGIFSPVANKKKGHGGGAG-DGGKDLHMFVWSS 339
G+ G D E+ + +G GG +++ G GG G DGG ++ + ++
Sbjct: 370 GAEGHDGEADDGEVDGVGGKEEDDV-------ALSDAHVGERGGDGVDGGPEVLVGELAA 212
Query: 340 SASPVSEGGIHVFRGGGDYGND 361
S S V EGG+ + GGD +D
Sbjct: 211 SGS-VDEGGLAMVGAGGDESSD 149
>BP043617
Length = 398
Score = 28.5 bits (62), Expect = 3.2
Identities = 18/50 (36%), Positives = 26/50 (52%), Gaps = 6/50 (12%)
Frame = +2
Query: 414 GSQGEAKPTN--MPPASVMTRLILIMVWRKLIRNP----NTYSSLIGLTW 457
G +GEA P N + S MTR +L+++W K + P SL +TW
Sbjct: 20 GEEGEAAPANPIIM*ES*MTRNLLVLIWVKSLLPP*MKIGGKESLYQITW 169
>AV418346
Length = 210
Score = 28.1 bits (61), Expect = 4.2
Identities = 16/51 (31%), Positives = 24/51 (46%)
Frame = -1
Query: 320 GHGGGAGDGGKDLHMFVWSSSASPVSEGGIHVFRGGGDYGNDQLNGVAHQK 370
G GGG+ GG + F +S A P EGG +GN + G+ ++
Sbjct: 129 GCGGGSSGGGSVVEDFEVTSVAEP*FEGG---------FGNAECKGIGRER 4
>TC8843
Length = 710
Score = 28.1 bits (61), Expect = 4.2
Identities = 18/62 (29%), Positives = 31/62 (49%), Gaps = 1/62 (1%)
Frame = +2
Query: 272 NVSPRQSNFGSLGFDEESGVAKANG-NGGNGYPAPHSAGIFSPVANKKKGHGGGAGDGGK 330
+ +P+Q + +G +G+ G NGG Y P + G+ + V G G G+G GG+
Sbjct: 29 STTPQQGSGTGIG----TGIGTGTGTNGGVVYTGPDT-GLGNSVPGTGSGLGSGSGSGGE 193
Query: 331 DL 332
+
Sbjct: 194 TM 199
>TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding protein 2,
partial (93%)
Length = 725
Score = 28.1 bits (61), Expect = 4.2
Identities = 16/48 (33%), Positives = 18/48 (37%)
Frame = +1
Query: 281 GSLGFDEESGVAKANGNGGNGYPAPHSAGIFSPVANKKKGHGGGAGDG 328
GS G+ G G GG GY G + G GGG G G
Sbjct: 409 GSRGYG--GGGGGYGGGGGGGYGGRREGGYGGDGGGSRYGSGGGGGGG 546
>BU494249
Length = 365
Score = 27.7 bits (60), Expect = 5.5
Identities = 25/93 (26%), Positives = 33/93 (34%), Gaps = 19/93 (20%)
Frame = +3
Query: 278 SNFGSLGFDEESGVAKANGNGGNGYPAPHSAGIFSPVANKKKGHGG----GAGDGGKDL- 332
S F + G N N + A +A + S A G GG G GDGG L
Sbjct: 63 SAFSVVTASTRGGFVNRNRRRSNVFSAA-AAAVRSTAAAAAGGGGGAAAAGRGDGGSSL* 239
Query: 333 --------------HMFVWSSSASPVSEGGIHV 351
H FVW S + V+ +H+
Sbjct: 240 CS*RSSAYGCPTRNHKFVWCSDSGRVTIWAVHM 338
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.138 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,395,748
Number of Sequences: 28460
Number of extensions: 151545
Number of successful extensions: 996
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 978
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 989
length of query: 591
length of database: 4,897,600
effective HSP length: 95
effective length of query: 496
effective length of database: 2,193,900
effective search space: 1088174400
effective search space used: 1088174400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC135795.7