BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135795.6 - phase: 0
(267 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC16664 similar to UP|BAD09830 (BAD09830) DNA-binding protein-li... 115 1e-26
BP062560 50 3e-07
TC17437 42 1e-04
TC8661 weakly similar to UP|O81348 (O81348) Symbiotic ammonium t... 32 0.11
TC18919 similar to GB|AAN73298.1|25141207|BT002301 At4g16430/dl4... 32 0.15
TC9411 similar to UP|Q41102 (Q41102) Phaseolin G-box binding pro... 32 0.15
AU251576 29 0.95
AV777521 28 1.6
TC8711 weakly similar to UP|VCLC_PEA (P13918) Vicilin precursor,... 27 2.8
AV776680 27 2.8
CB826773 27 3.6
TC10839 27 3.6
TC15321 similar to UP|BAD09664 (BAD09664) BHLH protein family-li... 27 3.6
BP036451 27 4.7
TC10885 27 4.7
BP082166 26 6.2
TC18477 26 6.2
TC9361 similar to UP|Q41123 (Q41123) PVPR3 protein, partial (68%) 26 6.2
AV415287 26 8.0
BP054147 26 8.0
>TC16664 similar to UP|BAD09830 (BAD09830) DNA-binding protein-like, partial
(22%)
Length = 1067
Score = 115 bits (287), Expect = 1e-26
Identities = 80/214 (37%), Positives = 116/214 (53%), Gaps = 15/214 (7%)
Frame = +1
Query: 61 EITDTPSQ-------QDRALAALKNHKEAEKRRRERINSHLDHLRTLLPCNSKTRQGFTS 113
E+ + P Q +R++ AL+NH EAE+RRR RIN+HLD LRT++P +K
Sbjct: 217 ELVEAPMQLERKGVSPERSIEALRNHSEAERRRRARINAHLDTLRTVIPGANKM------ 378
Query: 114 SKSSRTCERFKSKGSRLAKVVERVKELRQQTCQITQVETVPCEADGXTVISAGSDMSGEG 173
K S LA+V+ +KEL+ Q ++ +P + D V ++G
Sbjct: 379 -----------DKASLLAEVITHLKELKTNAAQASEGLMIPKDNDEIRVEEQEGGLNGFP 525
Query: 174 RLIFKASLCCEDRSDLIPDLIEILKSLHLKTLKAEMATLGGRTRNVLVVAAEKEHN--SI 231
I +ASLCCE + L+ D+ + L +LHL ++AE+ATLGGR +NV V+ + KE N
Sbjct: 526 YSI-RASLCCEYKPGLLSDIRQALDALHLMIMRAEIATLGGRMKNVFVIISCKEQNFEDA 702
Query: 232 ESIHFLQNS----LRSLLDRSSGCND--RSKRRR 259
E FL S LRS+LDR S D S++RR
Sbjct: 703 EYRQFLAGSVHQALRSVLDRFSVSQDILESRKRR 804
>BP062560
Length = 564
Score = 50.4 bits (119), Expect = 3e-07
Identities = 32/95 (33%), Positives = 57/95 (59%), Gaps = 12/95 (12%)
Frame = -1
Query: 177 FKASLCCEDRSDLIPDLIEILKSLHLKTLKAEMATLGGRTRNVLV-VAAEKEHNSIESIH 235
+ A++C + +S+++ DL + L +L L+ +KAE++TLG R +NV V +E+ +IE+
Sbjct: 537 YMATVCSDYQSEILSDLKQTLDALQLQLVKAELSTLGERMKNVFVFTCCXRENVNIEACQ 358
Query: 236 FL----QNSLRSLLDRSSG-------CNDRSKRRR 259
L +L S+L+++S +DR KRRR
Sbjct: 357 ALASNVHQALGSVLEKASNALEFSFRASDRCKRRR 253
>TC17437
Length = 455
Score = 42.0 bits (97), Expect = 1e-04
Identities = 32/72 (44%), Positives = 40/72 (55%), Gaps = 9/72 (12%)
Frame = +1
Query: 197 LKSLHLKTLKAEMATLGGRTRNVLVVAAEKEHN--SIESIHFLQNS----LRSLLDR--- 247
L SLHL +KA++ATLG R +NVLV+ + +E N E FL S LRS+L R
Sbjct: 19 LDSLHLMIIKADIATLGSRMKNVLVIISCEERNFEDAEYRQFLAGSVHPALRSVLHRFCV 198
Query: 248 SSGCNDRSKRRR 259
S KRRR
Sbjct: 199 SQEVIGTQKRRR 234
>TC8661 weakly similar to UP|O81348 (O81348) Symbiotic ammonium
transporter, partial (34%)
Length = 1007
Score = 32.0 bits (71), Expect = 0.11
Identities = 34/157 (21%), Positives = 69/157 (43%), Gaps = 10/157 (6%)
Frame = +1
Query: 77 KNHKEAEKRRRERINSHLDHLRTLLPCNSKTRQGFTSSKSSRTCERFKSKGSRLAKVVER 136
++H AE+RRRE+++ L L L+P K + + + + K RL + E+
Sbjct: 25 QDHIIAERRRREKLSQSLIALAALIPGLKKMDKASVLGDAIKYVKVLK---ERLRLLEEQ 195
Query: 137 VKELRQQTC------QITQVETVPCEADGXTVISAGSDMSG-EGRLIFK---ASLCCEDR 186
K ++ QI+ + D T+I + + E R+ K L C+ +
Sbjct: 196 NKNRAMESVVVVNKPQISNDDNSSSSCDDGTIIGSEEALPHVEARVSEKDVLLRLHCKKQ 375
Query: 187 SDLIPDLIEILKSLHLKTLKAEMATLGGRTRNVLVVA 223
L+ ++ +++LHL + + + G ++ +VA
Sbjct: 376 KGLLLKILFEIQNLHLFVVNSSVLPFGDSILDITIVA 486
>TC18919 similar to GB|AAN73298.1|25141207|BT002301 At4g16430/dl4240w
{Arabidopsis thaliana;}, partial (29%)
Length = 604
Score = 31.6 bits (70), Expect = 0.15
Identities = 14/29 (48%), Positives = 20/29 (68%)
Frame = +3
Query: 78 NHKEAEKRRRERINSHLDHLRTLLPCNSK 106
NH EAE++RRE++N LR ++P SK
Sbjct: 411 NHVEAERQRREKLNQRFYALRAVVPNISK 497
>TC9411 similar to UP|Q41102 (Q41102) Phaseolin G-box binding protein PG2
(Fragment), partial (30%)
Length = 705
Score = 31.6 bits (70), Expect = 0.15
Identities = 14/29 (48%), Positives = 20/29 (68%)
Frame = +2
Query: 78 NHKEAEKRRRERINSHLDHLRTLLPCNSK 106
NH EAE++RRE++N LR ++P SK
Sbjct: 188 NHVEAERQRREKLNQRFYALRAVVPNVSK 274
>AU251576
Length = 350
Score = 28.9 bits (63), Expect = 0.95
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = +1
Query: 178 KASLCCEDRSDLIPDLIEILKSLHLKTLKAEMAT 211
K LC EDR L+ D+ I + L ++AE+ T
Sbjct: 232 KLELCSEDRVGLLSDVTRIFRENGLTVIRAEVTT 333
>AV777521
Length = 433
Score = 28.1 bits (61), Expect = 1.6
Identities = 17/53 (32%), Positives = 25/53 (47%), Gaps = 3/53 (5%)
Frame = +2
Query: 101 LPCNSKTRQGFTSSKSSRTCERF---KSKGSRLAKVVERVKELRQQTCQITQV 150
+P N F S +SRTC RF + S ++KV + R + C IT +
Sbjct: 26 VPANRTFSIPFASFSASRTCSRFVLLTFRSSCISKVKPELPRPRCRLCSITPI 184
>TC8711 weakly similar to UP|VCLC_PEA (P13918) Vicilin precursor, partial
(74%)
Length = 1591
Score = 27.3 bits (59), Expect = 2.8
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = -2
Query: 102 PCNSKTRQGFTSSKSSRTCERFKSKGSRLAKVVERVKELRQQ 143
PC+S +R+G +SS C R S SR + V R E R++
Sbjct: 210 PCSSLSRRGCHGCRSSSPCSRQGS--SRCREAVARETETRKR 91
>AV776680
Length = 501
Score = 27.3 bits (59), Expect = 2.8
Identities = 12/41 (29%), Positives = 22/41 (53%)
Frame = +2
Query: 7 NNIGMENNSELFQFLVANNPSFFEYSTPSSTMMQQSFCSSS 47
NN+ +++ E + +N E+S PSST + C++S
Sbjct: 32 NNVNKKSHHEELSLIPSNLSHLCEFSMPSSTKLLTCSCTTS 154
>CB826773
Length = 428
Score = 26.9 bits (58), Expect = 3.6
Identities = 11/22 (50%), Positives = 17/22 (77%), Gaps = 2/22 (9%)
Frame = +3
Query: 41 QSFCSSS--DNNNNYYHPFEVS 60
QSFC+ S +NNN +HP+E++
Sbjct: 15 QSFCTLSAYKDNNNAFHPWEIA 80
>TC10839
Length = 769
Score = 26.9 bits (58), Expect = 3.6
Identities = 15/32 (46%), Positives = 18/32 (55%)
Frame = -2
Query: 90 INSHLDHLRTLLPCNSKTRQGFTSSKSSRTCE 121
INSHL +L LPC + Q T KS R C+
Sbjct: 561 INSHLSYLVLQLPC-WQFEQTCTIGKSKRECQ 469
>TC15321 similar to UP|BAD09664 (BAD09664) BHLH protein family-like, partial
(23%)
Length = 1593
Score = 26.9 bits (58), Expect = 3.6
Identities = 18/59 (30%), Positives = 27/59 (45%)
Frame = -1
Query: 21 LVANNPSFFEYSTPSSTMMQQSFCSSSDNNNNYYHPFEVSEITDTPSQQDRALAALKNH 79
L NN +F T+M + +NNNN YH ++I D P+ Q ++ NH
Sbjct: 1560 LYPNNQAF--------TIMPSKSSVTLNNNNNNYH---TNKIDDMPTLQRKSQYLQNNH 1417
>BP036451
Length = 511
Score = 26.6 bits (57), Expect = 4.7
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +2
Query: 80 KEAEKRRRERINSHLDHLRTLLPCNSK 106
K EK RRE++N LR+L+P +K
Sbjct: 326 KGTEKERREQLNGKYKILRSLIPSPTK 406
>TC10885
Length = 573
Score = 26.6 bits (57), Expect = 4.7
Identities = 19/71 (26%), Positives = 36/71 (49%)
Frame = +2
Query: 183 CEDRSDLIPDLIEILKSLHLKTLKAEMATLGGRTRNVLVVAAEKEHNSIESIHFLQNSLR 242
CE + +++ L++LHL + + G +V V+A + + +++ L NSLR
Sbjct: 191 CEKENGTELKILDHLENLHLSVNGSSVLPFGNSALSVTVIAQMGDAYGM-TMNDLVNSLR 367
Query: 243 SLLDRSSGCND 253
LL +S +D
Sbjct: 368 PLLLKSPMSSD 400
>BP082166
Length = 378
Score = 26.2 bits (56), Expect = 6.2
Identities = 11/36 (30%), Positives = 20/36 (55%)
Frame = +1
Query: 27 SFFEYSTPSSTMMQQSFCSSSDNNNNYYHPFEVSEI 62
+ F YS+ S++ SF SSS ++ Y P ++ +
Sbjct: 244 TLFSYSSSSTSASASSFASSSSSSPQPYPPIDLGTL 351
>TC18477
Length = 655
Score = 26.2 bits (56), Expect = 6.2
Identities = 11/24 (45%), Positives = 17/24 (70%)
Frame = +3
Query: 79 HKEAEKRRRERINSHLDHLRTLLP 102
H+E E++RR+ + S LR+LLP
Sbjct: 294 HREIERKRRQDMASFYASLRSLLP 365
>TC9361 similar to UP|Q41123 (Q41123) PVPR3 protein, partial (68%)
Length = 781
Score = 26.2 bits (56), Expect = 6.2
Identities = 11/24 (45%), Positives = 15/24 (61%)
Frame = +2
Query: 69 QDRALAALKNHKEAEKRRRERINS 92
+D L ++ HKE KRRR R N+
Sbjct: 278 RDLTLQMMRKHKEQPKRRRRRPNA 349
>AV415287
Length = 296
Score = 25.8 bits (55), Expect = 8.0
Identities = 10/23 (43%), Positives = 16/23 (69%)
Frame = +2
Query: 102 PCNSKTRQGFTSSKSSRTCERFK 124
P + K+ + F +SKS RTC+ F+
Sbjct: 17 PTSFKSFKSFNASKSFRTCQSFR 85
>BP054147
Length = 520
Score = 25.8 bits (55), Expect = 8.0
Identities = 24/99 (24%), Positives = 41/99 (41%), Gaps = 2/99 (2%)
Frame = +2
Query: 17 LFQFLVANNPSFFEYSTPSSTMMQQSFCSSSDNNNNYYHPFEVSEITDTPSQQDRALAAL 76
LFQFL+ S + S P ST S S ++ P+ + ++ +
Sbjct: 89 LFQFLL----SIYHLSLPRSTQQPHQNHSLSSPEDSQPRPWIIQKVKNP----------- 223
Query: 77 KNHKEAEKRRRERINSH--LDHLRTLLPCNSKTRQGFTS 113
H+ K+ +SH L + + PC TR+GF++
Sbjct: 224 --HRYXMKK-----HSHFPLSYFKNSHPCPPSTRRGFSN 319
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.129 0.360
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,329,879
Number of Sequences: 28460
Number of extensions: 53705
Number of successful extensions: 408
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 403
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 407
length of query: 267
length of database: 4,897,600
effective HSP length: 89
effective length of query: 178
effective length of database: 2,364,660
effective search space: 420909480
effective search space used: 420909480
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 54 (25.4 bits)
Medicago: description of AC135795.6