BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135604.8 + phase: 0 /pseudo
(1050 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18710 128 5e-30
TC18198 weakly similar to UP|Q9XGZ5 (Q9XGZ5) T1N24.20 protein, p... 95 5e-20
BP056651 49 3e-11
BP041113 65 6e-11
AV428268 53 3e-07
AV414139 52 6e-07
TC19704 weakly similar to UP|Q9SZ87 (Q9SZ87) RNA-directed DNA po... 52 6e-07
BP056597 51 1e-06
AV412895 49 3e-06
CN825682 48 7e-06
TC13572 44 2e-04
AV411435 41 0.001
TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%) 38 0.009
BP028574 37 0.016
TC15408 similar to UP|Q9LIC2 (Q9LIC2) Multispanning membrane pro... 37 0.021
CN825238 36 0.036
BE122611 35 0.047
AV418280 30 1.5
AV777945 30 2.0
AV768875 29 3.4
>TC18710
Length = 843
Score = 128 bits (321), Expect = 5e-30
Identities = 73/216 (33%), Positives = 117/216 (53%), Gaps = 3/216 (1%)
Frame = +2
Query: 324 GPDGINLGFFKSFWPEIKTDLVRFVTEFHRNGKLAKGINSTFIALIPKVASPQSLNEFRP 383
G DG+N F++ +K + + F ++ IN T + LIPKV P+S+N+FRP
Sbjct: 188 GMDGMNGLFYQKNGDVVKDSVT*AILAFLERAEIPNEINETLVTLIPKVPHPESINQFRP 367
Query: 384 ISLVGSMYKILAKILANRLRQVIGSVVSEAQSAFVKNRQILDGILI---AN*VVDEARKL 440
IS +YK+++KI RL+ + ++S QS F++ RQI D +LI A ++ L
Sbjct: 368 ISGCTFLYKVISKIFVARLKDAMPDLISPMQSGFIQGRQIQDNLLIVQEAFHAINRPGAL 547
Query: 441 KKYLLLFKVDFAKAYDSVDWGYLDEVMGSMSFPVVWRKWIKECVGTATASVLVNGSPTDE 500
+ + K+D KAYD V+W +L+ + + F W K I V + + +NG +
Sbjct: 548 GRNHSIIKLDMNKAYDRVEWKFLESSLLAFGFSTNWVKMIMILVSGVSYNYKINGVVGPK 727
Query: 501 FHLERGLRQGDPLSPFLFLLAA*GLNVLMKALVDAG 536
+RGLRQGDP SP+LFL L++L++ + G
Sbjct: 728 LLPQRGLRQGDPFSPYLFLFTMEVLSLLIQNSFNMG 835
>TC18198 weakly similar to UP|Q9XGZ5 (Q9XGZ5) T1N24.20 protein, partial
(14%)
Length = 592
Score = 95.1 bits (235), Expect = 5e-20
Identities = 60/198 (30%), Positives = 104/198 (52%), Gaps = 6/198 (3%)
Frame = -3
Query: 220 RLNWLRDGDANSKFFHSVLAARRRLNSLSSIL-VDGVVVEGVQPFRQAVFTHFKNHFLDP 278
R+ L+ GD N++FFH+ RR N + I G+ VEG + +A +F + +
Sbjct: 590 RIK*LK*GDHNTRFFHASTIQRRDFNRILKIKDAHGIWVEGQKKVNEAAVNYFSDIYSSS 411
Query: 279 RLER-----PGVGHLQFRKLSYLERGGLTKPFSEDEIKAAVWDCDSFKSPGPDGINLGFF 333
L+ + L +L++ L S DEIK AV+ K+PG DGIN F+
Sbjct: 410 PLQYLRECLAPIPKLVTDRLNHK----LCAQVSTDEIKEAVFSMGDLKAPGMDGINGLFY 243
Query: 334 KSFWPEIKTDLVRFVTEFHRNGKLAKGINSTFIALIPKVASPQSLNEFRPISLVGSMYKI 393
+ +K D+ + +F +G+L +N T + LIPK+ +++ +FRPIS +YK+
Sbjct: 242 QQNREIVKNDVNNAILDFFDHGELPVELNETLVTLIPKIPHAEAIQQFRPISCCSFIYKV 63
Query: 394 LAKILANRLRQVIGSVVS 411
++KI+ RL+ + +++S
Sbjct: 62 ISKIIVARLKPDMVNLIS 9
>BP056651
Length = 564
Score = 48.5 bits (114), Expect(2) = 3e-11
Identities = 20/42 (47%), Positives = 29/42 (68%)
Frame = +3
Query: 43 LIDLLLSGRKFTWFKGDGRSMRRLDRFLLSVEWCMVWPNCIQ 84
L D+ L GRK+TW + +G++ RLDRFL+S +W W +C Q
Sbjct: 432 LDDIPLVGRKYTWVRSNGKARSRLDRFLVSHQWNTSWXDCAQ 557
Score = 37.4 bits (85), Expect(2) = 3e-11
Identities = 18/38 (47%), Positives = 23/38 (60%), Gaps = 2/38 (5%)
Frame = +1
Query: 5 CVCGDFNTVRSVEERRSV--RGSQVVDDCTPFNDFIED 40
C+ GDFN+VR EERR GS + FNDFI++
Sbjct: 313 CMAGDFNSVRRSEERRGTHGEGSSGAGEMEEFNDFIDE 426
>BP041113
Length = 503
Score = 65.1 bits (157), Expect = 6e-11
Identities = 45/126 (35%), Positives = 53/126 (41%), Gaps = 1/126 (0%)
Frame = +1
Query: 858 LRWRRRLWAREEELVEECRALLLTVSLQESVTDRWLWVPNHDDGYSVRGVYDMLTSQEQP 917
L WRR L RE +E L V L E V D+WLW+P Y+V Y L +
Sbjct: 133 LSWRRALVGRELGWLELMMKDLSGVCLSEGVEDKWLWLPG--GTYTVNSAYSFLQAPTLA 306
Query: 918 QLHHNMELIWHK*VPLKVSILAWRLLRDRLPTKSNLANRGILALEARLCVSGC-GNVEDV 976
IW P V AWR L RLPT NL R ++ A C G VE V
Sbjct: 307 DTDPIFATIWRTVAP-SVKAFAWRCLLGRLPTYDNLIKRQVVVDPAMTVCKFCQGEVESV 483
Query: 977 NHLFLS 982
HL +
Sbjct: 484 THLLFA 501
>AV428268
Length = 429
Score = 52.8 bits (125), Expect = 3e-07
Identities = 30/98 (30%), Positives = 47/98 (47%)
Frame = +3
Query: 902 YSVRGVYDMLTSQEQPQLHHNMELIWHK*VPLKVSILAWRLLRDRLPTKSNLANRGILAL 961
YSV+ YD L Q + +L+W VP L+W++L +R+ TK NL RG++
Sbjct: 6 YSVKSAYDFLHGCANAQWDNIFKLLWSVKVPSNAIALSWKVLINRVQTKVNLNRRGVVI- 182
Query: 962 EARLCVSGCGNVEDVNHLFLSCVTFSALWPMVRAWLGV 999
+ +C + E +HL SC +W + GV
Sbjct: 183 -SNVCPLCSLDEESTDHLLFSCPIV*RIWSKISE*FGV 293
>AV414139
Length = 309
Score = 51.6 bits (122), Expect = 6e-07
Identities = 28/85 (32%), Positives = 37/85 (42%)
Frame = -2
Query: 859 RWRRRLWAREEELVEECRALLLTVSLQESVTDRWLWVPNHDDGYSVRGVYDMLTSQEQPQ 918
RWRR L RE+ + L V LQ+ DRW W + +D Y+VR Y +L
Sbjct: 260 RWRRELSVREQRQEADLIQALAGVCLQQGTEDRWKWTLDTEDIYTVRSAY*LLLDPIADS 81
Query: 919 LHHNMELIWHK*VPLKVSILAWRLL 943
E +W P + WRLL
Sbjct: 80 DSEVYEKVWVGIAPSNANAFVWRLL 6
>TC19704 weakly similar to UP|Q9SZ87 (Q9SZ87) RNA-directed DNA
polymerase-like protein, partial (3%)
Length = 530
Score = 51.6 bits (122), Expect = 6e-07
Identities = 35/107 (32%), Positives = 48/107 (44%)
Frame = -3
Query: 144 LKEKLKLMKVALKDWHAANTHNLPSKIAELKI*QASLDSKGEEEQLSEEELGELHGISTD 203
L K K+AL +WH N +IAELK L + S E+ E+
Sbjct: 459 LSGKTTNTKLALTEWHHQNFSRADKEIAELKNQLEHLQNYSN----SGEDWQEIKQRKMR 292
Query: 204 IHSLSRLITSICWQQSRLNWLRDGDANSKFFHSVLAARRRLNSLSSI 250
+ L R Q+SR+ WL+ GD N+KFFH+ RR N + I
Sbjct: 291 LKDLWRQEELFWSQRSRIKWLKSGDKNTKFFHASTVQRRERNRIERI 151
>BP056597
Length = 414
Score = 50.8 bits (120), Expect = 1e-06
Identities = 25/99 (25%), Positives = 44/99 (44%)
Frame = +3
Query: 860 WRRRLWAREEELVEECRALLLTVSLQESVTDRWLWVPNHDDGYSVRGVYDMLTSQEQPQL 919
W+ + + + + ++ + SL D W+W+ D YSV+ YD+L+ +
Sbjct: 102 WKNPISGEDVLKLGVMKQIVSSFSLINGKKDTWVWILEGDGQYSVKSAYDLLSGLDTTIG 281
Query: 920 HHNMELIWHK*VPLKVSILAWRLLRDRLPTKSNLANRGI 958
+W P L WR+ DR+ TK NL+ R +
Sbjct: 282 ISVFSKLWKACAPSNAVALGWRVFLDRIQTKDNLSRRHV 398
>AV412895
Length = 405
Score = 49.3 bits (116), Expect = 3e-06
Identities = 33/107 (30%), Positives = 48/107 (44%), Gaps = 2/107 (1%)
Frame = +1
Query: 901 GYSVRGVYDMLTSQEQPQLHHNMELIWHK*VPLKVSILAWRLLRDRLPTKSNLANRGIL- 959
G+SV + L Q + IW PL + WR+L R+ T+ NL R ++
Sbjct: 7 GFSVNSSFVFL*DQYLEEPDPVFN*IWMVPAPLNIKAFVWRVLLGRIQTRDNLLKRQVIH 186
Query: 960 -ALEARLCVSGCGNVEDVNHLFLSCVTFSALWPMVRAWLGVVGSRFS 1005
AL+A + G E +HL SC +W AWLGV ++ S
Sbjct: 187 NALDAICPLCGLAE-ESGSHLLFSCAESMLIWYECFAWLGVSTAQVS 324
>CN825682
Length = 626
Score = 48.1 bits (113), Expect = 7e-06
Identities = 31/92 (33%), Positives = 44/92 (47%), Gaps = 3/92 (3%)
Frame = -3
Query: 8 GDFNTVRSVEERRSVRGSQVVDDCTPFNDFIEDRVLIDLLLSGRKFTWFKGDGRSM---R 64
GDFN + +E+ R Q F DF++ L+DL L G +FTW +
Sbjct: 288 GDFNEILHHQEKEGAR-DQDPTRIQVFRDFLDGANLMDLELKGCRFTWLSNLRNGLVTKE 112
Query: 65 RLDRFLLSVEWCMVWPNCIQVAQLRGLSDHCP 96
RLDR L++ W + +PN +A SDH P
Sbjct: 111 RLDRVLVNWTWRLDYPNATAMALPIISSDHSP 16
>TC13572
Length = 534
Score = 43.5 bits (101), Expect = 2e-04
Identities = 22/60 (36%), Positives = 31/60 (51%), Gaps = 1/60 (1%)
Frame = -3
Query: 859 RWRRRLWAREEELVEECRALLLTVSLQESVTDRWLWVPNHDDGYSVRGVYDML-TSQEQP 917
RWRR L RE + ++ ++ V L V D+W+W P+ D YSV + L EQP
Sbjct: 193 RWRRPLAGRELDWLQALSLDIVRVPLLAGVPDKWVWKPSEDGSYSVNSAFIFLQVPSEQP 14
>AV411435
Length = 426
Score = 40.8 bits (94), Expect = 0.001
Identities = 30/110 (27%), Positives = 47/110 (42%), Gaps = 11/110 (10%)
Frame = +3
Query: 892 WLWVPNHDDGYSVRGVYDMLTSQE------QPQLHHNMELIWHK*----VPLKVSILAWR 941
W ++P+ + YSV+ Y + + + H +WH VP KV WR
Sbjct: 51 WPFIPSGE--YSVKSGYRAVKATQFNLSNLASSSSHPSSFLWHTIWGAQVPKKVRSFLWR 224
Query: 942 LLRDRLPTKSNLANRGILALE-ARLCVSGCGNVEDVNHLFLSCVTFSALW 990
+ +P K NL R + + +C G ED+NH L+C A+W
Sbjct: 225 AANNAIPVKRNLKRRNMGRDDFCPICNKG---PEDINHALLACEWTRAVW 365
>TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%)
Length = 912
Score = 37.7 bits (86), Expect = 0.009
Identities = 24/83 (28%), Positives = 45/83 (53%)
Frame = -3
Query: 545 RADSVVVSHLQFADDTLLIGNKSWANVRALRAGLVMFEAMSGLKVNFHKSSLVGVNINDS 604
R + +SHL FAD+ ++ N ++ + L +F+ +SGL N KS V + + ++
Sbjct: 391 RCQNEEISHLCFADNLMVFSNGYLESIAIINNVLHIFQHLSGLTPNPAKSE-VFILVKET 215
Query: 605 WLSEAASVLGCKVVKIPFLYLGL 627
++ ++LG K K+P LG+
Sbjct: 214 TKNQITNMLGYKEGKLPVR*LGV 146
>BP028574
Length = 454
Score = 37.0 bits (84), Expect = 0.016
Identities = 16/64 (25%), Positives = 30/64 (46%)
Frame = +3
Query: 890 DRWLWVPNHDDGYSVRGVYDMLTSQEQPQLHHNMELIWHK*VPLKVSILAWRLLRDRLPT 949
D W+W+ + ++V+ Y+ L + Q + +W P LAWR+ +R+P
Sbjct: 261 DSWVWIKDGSGSFTVKSAYEELHGEIQEIEDNFFRKLWQSKAPSNSIALAWRVGLNRVPC 440
Query: 950 KSNL 953
+ L
Sbjct: 441 MAEL 452
>TC15408 similar to UP|Q9LIC2 (Q9LIC2) Multispanning membrane protein-like,
partial (10%)
Length = 879
Score = 36.6 bits (83), Expect = 0.021
Identities = 20/67 (29%), Positives = 27/67 (39%)
Frame = -1
Query: 861 RRRLWAREEELVEECRALLLTVSLQESVTDRWLWVPNHDDGYSVRGVYDMLTSQEQPQLH 920
RR RE LV + LL +V L E D W W N + Y+V+ Y L +
Sbjct: 747 RRNPSTRENLLVADLCNLLESVPLSEDTNDSWSWTANPEGQYTVQTAYKFLRASSNEFTD 568
Query: 921 HNMELIW 927
+W
Sbjct: 567 PIFHFVW 547
>CN825238
Length = 692
Score = 35.8 bits (81), Expect = 0.036
Identities = 28/109 (25%), Positives = 52/109 (47%), Gaps = 7/109 (6%)
Frame = +3
Query: 857 ALRWRRRLWAREEELVEECRALLLTVSLQESVT-DRWLWVPNHDDGYSVRGVYDMLTSQE 915
A RW +L ++ +E A++ ++ L T + LW + + +SVR Y + +
Sbjct: 312 ARRWNVQLI--DQLFPKETAAVIKSIPLGWRTTRNSCLWRWSRNGAFSVRSAYYNIQVAK 485
Query: 916 QPQLHHNM------ELIWHK*VPLKVSILAWRLLRDRLPTKSNLANRGI 958
+ Q + + +W VP K+ A+R+ R+ +P + NL RGI
Sbjct: 486 EMQTSSSAASSFPWKKLWRTPVPNKMKHFAYRVARNIIPCRENLQRRGI 632
>BE122611
Length = 357
Score = 35.4 bits (80), Expect = 0.047
Identities = 20/74 (27%), Positives = 34/74 (45%)
Frame = -3
Query: 889 TDRWLWVPNHDDGYSVRGVYDMLTSQEQPQLHHNMELIWHK*VPLKVSILAWRLLRDRLP 948
TDR LW + YS + Y++L ++ L+W P KV W + R+ LP
Sbjct: 334 TDRILWTEESNGCYSAKSTYNLLI-EDGGNESRFWSLVWKANAPEKVRFFLWLVGRNALP 158
Query: 949 TKSNLANRGILALE 962
+ + + +L L+
Sbjct: 157 SNARRHHITLLVLQ 116
>AV418280
Length = 398
Score = 30.4 bits (67), Expect = 1.5
Identities = 12/31 (38%), Positives = 18/31 (57%), Gaps = 1/31 (3%)
Frame = -2
Query: 970 CGNVEDVN-HLFLSCVTFSALWPMVRAWLGV 999
C VE+ + HLF +CV +W + WLG+
Sbjct: 379 CTTVEECSGHLFFTCVFSMGVWQALHRWLGI 287
>AV777945
Length = 279
Score = 30.0 bits (66), Expect = 2.0
Identities = 15/51 (29%), Positives = 25/51 (48%), Gaps = 1/51 (1%)
Frame = +1
Query: 910 MLTSQEQPQLHHNM-ELIWHK*VPLKVSILAWRLLRDRLPTKSNLANRGIL 959
+LT +Q + H + + +W P LAW +L +L TK L R ++
Sbjct: 109 LLTQLKQLRSHEGLFDFLWASKAPSNSLALAWNVLIKKLQTKDELRKRNVI 261
>AV768875
Length = 232
Score = 29.3 bits (64), Expect = 3.4
Identities = 16/38 (42%), Positives = 21/38 (55%)
Frame = +1
Query: 20 RSVRGSQVVDDCTPFNDFIEDRVLIDLLLSGRKFTWFK 57
R+ R + V+DDC LIDL + G KFTWF+
Sbjct: 130 RAQRFATVLDDCN----------LIDLGMVGGKFTWFR 213
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.338 0.149 0.505
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,683,873
Number of Sequences: 28460
Number of extensions: 327948
Number of successful extensions: 2443
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 2384
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2435
length of query: 1050
length of database: 4,897,600
effective HSP length: 100
effective length of query: 950
effective length of database: 2,051,600
effective search space: 1949020000
effective search space used: 1949020000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 60 (27.7 bits)
Medicago: description of AC135604.8