BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135566.8 - phase: 0
(269 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC15097 157 2e-39
TC10549 114 2e-26
TC11039 weakly similar to UP|O65074 (O65074) YGL010w-like protei... 36 0.006
TC11860 weakly similar to UP|Q40380 (Q40380) Arabinogalactan-pro... 35 0.013
TC15328 weakly similar to UP|Q9LP71 (Q9LP71) T1N15.15, partial (... 33 0.066
AV775527 32 0.087
TC11009 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {Ar... 31 0.19
TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (... 31 0.25
TC13802 similar to UP|Q9M1T1 (Q9M1T1) Sugar-phosphate isomerase-... 31 0.25
TC17083 similar to UP|Q84XA0 (Q84XA0) Early tobacco anther 1, pa... 30 0.33
TC14217 30 0.33
TC11494 similar to UP|Q7X9B2 (Q7X9B2) 9/13 hydroperoxide lyase, ... 30 0.33
TC13094 similar to UP|Q94JM3 (Q94JM3) AT5g62000/mtg10_20, partia... 30 0.33
BP036655 30 0.43
TC15944 similar to UP|Q9ZST8 (Q9ZST8) Tic20, partial (46%) 30 0.43
TC15784 similar to UP|PE31_ARATH (Q9LHA7) Peroxidase 31 precurso... 30 0.56
TC12785 similar to UP|Q7X9B2 (Q7X9B2) 9/13 hydroperoxide lyase, ... 30 0.56
TC8244 similar to PIR|S18343|DSPMN superoxide dismutase (Mn) pr... 30 0.56
BP043633 29 0.96
TC15782 homologue to PIR|T44259|T44259 lipoic acid synthase [im... 29 0.96
>TC15097
Length = 489
Score = 157 bits (397), Expect = 2e-39
Identities = 73/98 (74%), Positives = 87/98 (88%), Gaps = 1/98 (1%)
Frame = +3
Query: 1 MACALQATITTNTCAFSTRKFSLKQQKINKRSSCMFIVRASSE-SDCNDEECAPDKEVGK 59
M+CALQAT+ +NTCAFS+R+ SL QK +KRSS + VRA+S+ +DCNDEECAPDKEVGK
Sbjct: 195 MSCALQATLASNTCAFSSRRLSLNNQKFSKRSSYLLTVRANSDDADCNDEECAPDKEVGK 374
Query: 60 VSMEWLAEEKTKVVGTYPPRRKQGWTGYVEKDTAGQTN 97
+S+EWLA EKTKVVGT+PPRR +GWTGYVEKDTAGQTN
Sbjct: 375 ISVEWLAGEKTKVVGTFPPRRAKGWTGYVEKDTAGQTN 488
>TC10549
Length = 631
Score = 114 bits (284), Expect = 2e-26
Identities = 80/145 (55%), Positives = 94/145 (64%), Gaps = 1/145 (0%)
Frame = +3
Query: 126 NTAAIAAGLALISIAAASSILLQVGKNSPPQVQKVEYSGPSLSYYINEFKTQEITQAPSV 185
NTA I A LALISIAAASSILLQVGK +PP +Q+ EYSGPSLSYYI++FK EITQAP++
Sbjct: 3 NTAGIVAFLALISIAAASSILLQVGKTTPP-IQRAEYSGPSLSYYISKFKPPEITQAPAL 179
Query: 186 STETELSSSVPTESDVPTQTELSSSVQSESIAPAKTELSSSIQTESVATDASISVPPESS 245
E EL SSV P E+SSSV +E S IQ E V PESS
Sbjct: 180 -IEAELPSSVVQPETAP---EVSSSVAAE----------SEIQPEV----PEPVVQPESS 305
Query: 246 PAEV-SQVQVESQIQTETSSVDIVS 269
P +V S V+V S+IQ E +SV +VS
Sbjct: 306 PPQVSSSVEVGSEIQPEPASVSVVS 380
>TC11039 weakly similar to UP|O65074 (O65074) YGL010w-like protein, partial
(79%)
Length = 733
Score = 36.2 bits (82), Expect = 0.006
Identities = 30/89 (33%), Positives = 38/89 (41%), Gaps = 3/89 (3%)
Frame = +1
Query: 180 TQAPSVSTETELSSSVPTESDVPTQTELSSSVQSESIAPAKTELSSSIQTESVATDASIS 239
TQ+ S ST + S S T LSS S I T SSS + +T IS
Sbjct: 88 TQSTSQSTSSSFGQSSSPLSSSSTSHHLSSLPSSPIIPLFSTSASSSQSSTLCSTPLWIS 267
Query: 240 VP---PESSPAEVSQVQVESQIQTETSSV 265
P P SSP +QV SQ + + S+
Sbjct: 268 KPVPSPRSSPFSAGSLQVSSQTPSASHSL 354
>TC11860 weakly similar to UP|Q40380 (Q40380) Arabinogalactan-protein
precursor, partial (17%)
Length = 492
Score = 35.0 bits (79), Expect = 0.013
Identities = 24/95 (25%), Positives = 48/95 (50%)
Frame = +2
Query: 174 FKTQEITQAPSVSTETELSSSVPTESDVPTQTELSSSVQSESIAPAKTELSSSIQTESVA 233
F + + Q+P+ S S++ P ++ PT T +S +V +P+ TE S + ++
Sbjct: 29 FLSSTMAQSPASSPTKSESAAPPRKALSPTATPVSPAV-----SPSSTEESPAAVPAAI- 190
Query: 234 TDASISVPPESSPAEVSQVQVESQIQTETSSVDIV 268
T +SIS PP +P + V +++ S+ ++
Sbjct: 191 TPSSISGPPSEAPGPAANGAVLNRVAVSGSAAIVI 295
>TC15328 weakly similar to UP|Q9LP71 (Q9LP71) T1N15.15, partial (50%)
Length = 1325
Score = 32.7 bits (73), Expect = 0.066
Identities = 23/63 (36%), Positives = 31/63 (48%)
Frame = +1
Query: 153 SPPQVQKVEYSGPSLSYYINEFKTQEITQAPSVSTETELSSSVPTESDVPTQTELSSSVQ 212
SPP V K P+L + I +T S ST T SS+PT +PT+T+ SS
Sbjct: 160 SPPPVPK---PSPTLRFPIPPMMRYPLTNGTS*STSTR--SSMPTLKSLPTRTKCLSSAS 324
Query: 213 SES 215
S +
Sbjct: 325 SST 333
>AV775527
Length = 466
Score = 32.3 bits (72), Expect = 0.087
Identities = 26/73 (35%), Positives = 37/73 (50%), Gaps = 11/73 (15%)
Frame = +1
Query: 185 VSTETELSSSVPTESDVPT----QTELSSSVQSESIA------PAKTELS-SSIQTESVA 233
VST T SS +P+ S VPT T L + S +++ P ++ LS S+ + A
Sbjct: 160 VSTPTSTSSPMPSPSPVPTCASRATHLPAPASSSAVSHSP*PPPPQSPLSFHSLFPPTPA 339
Query: 234 TDASISVPPESSP 246
T +S S PP SP
Sbjct: 340 TASSSSSPPADSP 378
>TC11009 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {Arabidopsis
thaliana;}, partial (44%)
Length = 700
Score = 31.2 bits (69), Expect = 0.19
Identities = 29/120 (24%), Positives = 48/120 (39%)
Frame = -2
Query: 122 DGAQNTAAIAAGLALISIAAASSILLQVGKNSPPQVQKVEYSGPSLSYYINEFKTQEITQ 181
DG + + L+ S ++ S L ++ V++ YS P S + +
Sbjct: 366 DGTPAFSPSSLSLSSPSSSSGSKSLDASSSSAAATVRRTRYSVPKSSS-----SSSSSSS 202
Query: 182 APSVSTETELSSSVPTESDVPTQTELSSSVQSESIAPAKTELSSSIQTESVATDASISVP 241
+ S S+ + S P P + SSS S S P + SSS + S + +S S P
Sbjct: 201 SSSSSSSSSGSPPPPRPPSTPPSSSSSSSSSSSSPPPGASSSSSSSPSSSSPSSSSSSSP 22
Score = 28.1 bits (61), Expect = 1.6
Identities = 22/95 (23%), Positives = 43/95 (45%), Gaps = 1/95 (1%)
Frame = -2
Query: 175 KTQEITQAPSVSTETELSSSVP-TESDVPTQTELSSSVQSESIAPAKTELSSSIQTESVA 233
K+ + + + + +T SVP + S + + SSS S S +P S+ + S +
Sbjct: 300 KSLDASSSSAAATVRRTRYSVPKSSSSSSSSSSSSSSSSSSSGSPPPPRPPSTPPSSSSS 121
Query: 234 TDASISVPPESSPAEVSQVQVESQIQTETSSVDIV 268
+ +S S PP + + S S + +SS ++
Sbjct: 120 SSSSSSSPPPGASSSSSSSPSSSSPSSSSSSSPLI 16
>TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (SPBC3D6.14C
protein) (Fragment), partial (15%)
Length = 543
Score = 30.8 bits (68), Expect = 0.25
Identities = 20/58 (34%), Positives = 32/58 (54%)
Frame = -3
Query: 183 PSVSTETELSSSVPTESDVPTQTELSSSVQSESIAPAKTELSSSIQTESVATDASISV 240
PS ST SS+P+ S + + SSS S S + + + SSS + S ++ +S+SV
Sbjct: 400 PSTST-----SSLPSSSSSSSSSSSSSSPSSSSSSSSSSSSSSSSSSSSSSSSSSLSV 242
>TC13802 similar to UP|Q9M1T1 (Q9M1T1) Sugar-phosphate isomerase-like
protein, partial (41%)
Length = 522
Score = 30.8 bits (68), Expect = 0.25
Identities = 20/67 (29%), Positives = 33/67 (48%)
Frame = +2
Query: 181 QAPSVSTETELSSSVPTESDVPTQTELSSSVQSESIAPAKTELSSSIQTESVATDASISV 240
+APS S + +S PT+S P+ S+ S + P+ T S S T + ++ ++
Sbjct: 191 RAPSSSPASASPASSPTKSPKPSSPSASAPPSSTPLTPS-TATSESSPTATSSSSSASPA 367
Query: 241 PPESSPA 247
PP S A
Sbjct: 368 PPTSFSA 388
>TC17083 similar to UP|Q84XA0 (Q84XA0) Early tobacco anther 1, partial (67%)
Length = 831
Score = 30.4 bits (67), Expect = 0.33
Identities = 19/59 (32%), Positives = 32/59 (54%), Gaps = 1/59 (1%)
Frame = -1
Query: 202 PTQTELSSSVQSESI-APAKTELSSSIQTESVATDASISVPPESSPAEVSQVQVESQIQ 259
P S S S S+ APA+T L+++ T SV+ +I PP + A S + E++++
Sbjct: 498 PQHAPPSPSTTSPSLTAPARTALATAASTSSVSRHKTIPFPPVFAGAVESTARSETRME 322
>TC14217
Length = 985
Score = 30.4 bits (67), Expect = 0.33
Identities = 23/91 (25%), Positives = 40/91 (43%)
Frame = +1
Query: 175 KTQEITQAPSVSTETELSSSVPTESDVPTQTELSSSVQSESIAPAKTELSSSIQTESVAT 234
K EI+ A S L+S E + T + + Q E P KTE+ + +++ T
Sbjct: 256 KVDEISTAISEPVRETLASKFKDEESIETGVDNTEKEQVEE--PVKTEVQDTKESDVTKT 429
Query: 235 DASISVPPESSPAEVSQVQVESQIQTETSSV 265
+ P+ +PA+ +Q Q + I S+
Sbjct: 430 SKDL---PKETPAKPAQKQSNNIISKVKQSL 513
>TC11494 similar to UP|Q7X9B2 (Q7X9B2) 9/13 hydroperoxide lyase, partial
(27%)
Length = 541
Score = 30.4 bits (67), Expect = 0.33
Identities = 24/85 (28%), Positives = 34/85 (39%)
Frame = +2
Query: 163 SGPSLSYYINEFKTQEITQAPSVSTETELSSSVPTESDVPTQTELSSSVQSESIAPAKTE 222
S PS + P S T SS PT P+ S +S S AP
Sbjct: 194 SAPSATATTTSTTKAATPSLPPESKNTTPLSSEPTCLPAPSSPPTLESSRS-STAPLSPS 370
Query: 223 LSSSIQTESVATDASISVPPESSPA 247
S++ ++ S + ++S PP SPA
Sbjct: 371 SSTTTRSRSETSSTTLSCPPLPSPA 445
>TC13094 similar to UP|Q94JM3 (Q94JM3) AT5g62000/mtg10_20, partial (18%)
Length = 685
Score = 30.4 bits (67), Expect = 0.33
Identities = 22/69 (31%), Positives = 38/69 (54%), Gaps = 4/69 (5%)
Frame = +2
Query: 166 SLSYYINEFKTQEITQAPSVSTETELSSS---VPTESDVPTQTELSSS-VQSESIAPAKT 221
+LS+YI++ + ++ + + T+TEL SS V E V + TE++SS V + P
Sbjct: 110 TLSFYIHKKREEQNSVQTEIETKTELRSSEERVSDERSVVSFTEMASSEVAMKGNCPNHG 289
Query: 222 ELSSSIQTE 230
+ S+ TE
Sbjct: 290 DGGRSVTTE 316
>BP036655
Length = 556
Score = 30.0 bits (66), Expect = 0.43
Identities = 28/97 (28%), Positives = 44/97 (44%), Gaps = 16/97 (16%)
Frame = +1
Query: 162 YSGPSLSYYINEFKTQEITQAPSVSTETELSSSVPTESDVPTQTELSSSVQSESIAPAKT 221
+S P S + F I+ + S ST + LSSS+ + S + + T SSS + S +P+
Sbjct: 220 FSTPHSSSSSSSFSVS-ISVSLSSSTSSSLSSSLISSSSLHSSTTSSSSSTTSSSSPSSE 396
Query: 222 ELSSSIQT----------------ESVATDASISVPP 242
SSS + E AT + +S+PP
Sbjct: 397 RPSSSNRCSLSLPSPRSA*KQGRGEGEATASKMSLPP 507
>TC15944 similar to UP|Q9ZST8 (Q9ZST8) Tic20, partial (46%)
Length = 619
Score = 30.0 bits (66), Expect = 0.43
Identities = 23/110 (20%), Positives = 50/110 (44%), Gaps = 8/110 (7%)
Frame = +1
Query: 82 QGWTGYVEKDTAGQTNIYSVEPAVYVAESAISSGTAGTSSDGAQNTAAIAAGLALISIAA 141
QG+ + G ++++S A + S + ++ + + G+AL ++++
Sbjct: 106 QGYAPLNTRPCRGSSSVFSC--ASQLPAKVAVSNIRSSWGHSLESKSLVPRGIALSNMSS 279
Query: 142 ASSILLQVGKN--------SPPQVQKVEYSGPSLSYYINEFKTQEITQAP 183
ASS+LL G+N PP+V+K + + + F+ +T+ P
Sbjct: 280 ASSLLLSGGQNLLFRTVPVLPPRVRKSSMAPRAAKDVPSSFRFPPMTKKP 429
>TC15784 similar to UP|PE31_ARATH (Q9LHA7) Peroxidase 31 precursor (Atperox
P31) (ATP41) , partial (38%)
Length = 475
Score = 29.6 bits (65), Expect = 0.56
Identities = 22/66 (33%), Positives = 31/66 (46%)
Frame = +1
Query: 182 APSVSTETELSSSVPTESDVPTQTELSSSVQSESIAPAKTELSSSIQTESVATDASISVP 241
AP S + ++S PT + P SSS Q S P T S+S + +T +S P
Sbjct: 202 APPRSASSSTTASTPTAATPP-----SSSPQLPSTKPNATPTSTSPSPATPSTSSSELKP 366
Query: 242 PESSPA 247
P +S A
Sbjct: 367 PSNSLA 384
>TC12785 similar to UP|Q7X9B2 (Q7X9B2) 9/13 hydroperoxide lyase, partial
(24%)
Length = 424
Score = 29.6 bits (65), Expect = 0.56
Identities = 25/100 (25%), Positives = 41/100 (41%)
Frame = +1
Query: 148 QVGKNSPPQVQKVEYSGPSLSYYINEFKTQEITQAPSVSTETELSSSVPTESDVPTQTEL 207
Q SP V+ + S + + E + +S+E + +P S PT L
Sbjct: 94 QAATASPSSVRSATATTTSTTKAVTPSSPPE*KKTTPLSSEP---TCLPAPSYPPTLELL 264
Query: 208 SSSVQSESIAPAKTELSSSIQTESVATDASISVPPESSPA 247
SS AP S++ ++ S + ++S PP SPA
Sbjct: 265 RSST-----APRSPSCSTTTRSRSATSSTALSCPPPPSPA 369
>TC8244 similar to PIR|S18343|DSPMN superoxide dismutase (Mn) precursor -
garden pea {Pisum sativum;} , partial
(90%)
Length = 1168
Score = 29.6 bits (65), Expect = 0.56
Identities = 23/53 (43%), Positives = 32/53 (59%), Gaps = 2/53 (3%)
Frame = +3
Query: 176 TQEITQAPSVSTETELSSS--VPTESDVPTQTELSSSVQSESIAPAKTELSSS 226
T+ IT+ S +T T LSSS +P+ S +P Q LSS VQS S+A + + S
Sbjct: 240 TRSITRLTSPTT-TRLSSSFKMPSPSPIP-QPSLSSRVQSNSMAEVMSTIPFS 392
>BP043633
Length = 542
Score = 28.9 bits (63), Expect = 0.96
Identities = 20/59 (33%), Positives = 30/59 (49%)
Frame = +3
Query: 183 PSVSTETELSSSVPTESDVPTQTELSSSVQSESIAPAKTELSSSIQTESVATDASISVP 241
P +S + S P+ + T T SSSV+ + P +SSS + S ++ AS SVP
Sbjct: 345 PPISVPPPIPDSFPSTAVGATTTAASSSVEPLFLPP--QAVSSSSSSSSSSSPASSSVP 515
>TC15782 homologue to PIR|T44259|T44259 lipoic acid synthase [imported] -
Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (38%)
Length = 544
Score = 28.9 bits (63), Expect = 0.96
Identities = 14/39 (35%), Positives = 22/39 (55%)
Frame = -1
Query: 105 VYVAESAISSGTAGTSSDGAQNTAAIAAGLALISIAAAS 143
+Y+A+SA SS + + D ++ +AAG AL I S
Sbjct: 442 LYIAKSATSSASVSKNHDSSRGGTGLAAGPALTQIRTLS 326
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.303 0.118 0.308
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,740,343
Number of Sequences: 28460
Number of extensions: 45158
Number of successful extensions: 313
Number of sequences better than 10.0: 72
Number of HSP's better than 10.0 without gapping: 249
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 286
length of query: 269
length of database: 4,897,600
effective HSP length: 89
effective length of query: 180
effective length of database: 2,364,660
effective search space: 425638800
effective search space used: 425638800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (21.9 bits)
S2: 54 (25.4 bits)
Medicago: description of AC135566.8