BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135504.5 - phase: 0 /pseudo
(1283 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP063694 76 3e-14
TC8301 71 1e-12
BP043850 69 6e-12
BP084332 68 1e-11
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 65 7e-11
AV766088 63 3e-10
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 61 1e-09
AV780009 59 5e-09
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 40 7e-09
BP033602 57 2e-08
BP057233 56 4e-08
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 55 9e-08
AV778782 50 3e-06
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 45 6e-05
TC9039 45 6e-05
TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprot... 37 2e-04
BP058111 43 4e-04
TC8302 42 6e-04
BP054557 40 0.002
AV764934 39 0.004
>BP063694
Length = 511
Score = 76.3 bits (186), Expect = 3e-14
Identities = 42/118 (35%), Positives = 64/118 (53%), Gaps = 1/118 (0%)
Frame = -2
Query: 418 KLWHLRLGHVSERGLVELAKQGLLG-NEKLNKLDFCDNCTLGKQHKVKFGVGVHKSSRPF 476
+LWH RLGHV+ + +L K G L + L K C +C + K ++ F ++S
Sbjct: 363 ELWHSRLGHVNFDIIKQLHKHGCLDVSSILPKPICCTSCQMAKSKRLVFHDNNKRASAVL 184
Query: 477 E*VHSDLLGPA*VKTYGGGSYFTSIIDDYSRRVWVYILKNKSDAFEKFKEWDILVENQ 534
+ +H DL GP+ V + G SYF +DD+SR W Y LK KSD + + + +EN+
Sbjct: 183 DLIHCDLRGPSPVASIDGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLLRFKVFMENR 10
>TC8301
Length = 1243
Score = 70.9 bits (172), Expect = 1e-12
Identities = 40/105 (38%), Positives = 66/105 (62%)
Frame = +2
Query: 2 MGSKWDIEKFTGSNLFGLWKVKMRAILIQEKCVEALKREAQMSAHLTPAEKTEMNDKAVS 61
M +K++I KF+GSN F LWK+K++AIL ++ C+ A+ A +T + EM+D AV+
Sbjct: 500 MAAKFEIPKFSGSN-FSLWKLKIKAILRKDNCLPAIDGRP---ADITDDKWKEMDDNAVA 667
Query: 62 AIILCLGDKMLREVSRETTAVSMWNKLDLLYMTKSLAHRQCLKQQ 106
+ L + D +L ++ + TA +W+ L LY KSL H + LK++
Sbjct: 668 NLHLAVADSVLSSIAEKKTAKEIWDTLIQLYEVKSL-HNRILKEE 799
>BP043850
Length = 515
Score = 68.6 bits (166), Expect = 6e-12
Identities = 34/84 (40%), Positives = 51/84 (60%), Gaps = 1/84 (1%)
Frame = -1
Query: 1190 EAIWLKGMIGEMGISQGC-VKIHCDSQSAIHLANHQIYHERTKHINIRLHFVRDMIETKE 1248
E IWL+G++ E+G Q +H D+ SAI +A + +YHE T+HI + H VR+ + +
Sbjct: 512 EIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRRV 333
Query: 1249 IKVEKVASEENPADIFTKSLPRSR 1272
I + V++ ADI TKSL R R
Sbjct: 332 ITLPHVSTSVQIADILTKSLTRQR 261
>BP084332
Length = 368
Score = 67.8 bits (164), Expect = 1e-11
Identities = 30/88 (34%), Positives = 52/88 (59%)
Frame = +2
Query: 1190 EAIWLKGMIGEMGISQGCVKIHCDSQSAIHLANHQIYHERTKHINIRLHFVRDMIETKEI 1249
+ +W+K + + I + + I+CD+ +AI L+ + I H R KHI ++ HF+RD ++ +
Sbjct: 95 QMLWMKHQLEDYQILESNIPIYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYVQKGVL 274
Query: 1250 KVEKVASEENPADIFTKSLPRSRFKHCL 1277
++ V ++ ADIFTK L RF L
Sbjct: 275 LLKFVDTDHQWADIFTKPLAEDRFNFIL 358
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 65.1 bits (157), Expect = 7e-11
Identities = 28/74 (37%), Positives = 46/74 (61%)
Frame = +1
Query: 1210 IHCDSQSAIHLANHQIYHERTKHINIRLHFVRDMIETKEIKVEKVASEENPADIFTKSLP 1269
++CDSQSA H+A + ++HERTKH++I H VR+ ++ K + ++S + ADI TK L
Sbjct: 67 LYCDSQSARHIATNAVFHERTKHLDIDCHVVREKLQAKLFHLLPISSVDQTADILTKPLE 246
Query: 1270 RSRFKHCLDLINFI 1283
F H + + +
Sbjct: 247 SGPFSHLVSKLGVL 288
>AV766088
Length = 501
Score = 63.2 bits (152), Expect = 3e-10
Identities = 34/103 (33%), Positives = 52/103 (50%)
Frame = -3
Query: 419 LWHLRLGHVSERGLVELAKQGLLGNEKLNKLDFCDNCTLGKQHKVKFGVGVHKSSRPFE* 478
LWH RLGH S L L + E LN C++C GK ++ F + + PF+
Sbjct: 307 LWHSRLGHPSSSALRYLRSNKFISYELLNYSPVCESCVFGKHVRLPFVSSNNVTVMPFDI 128
Query: 479 VHSDLLGPA*VKTYGGGSYFTSIIDDYSRRVWVYILKNKSDAF 521
+HSD L + V + G ++ +DD++ +W + L NKS F
Sbjct: 127 LHSD-LWTSPVLSSAGHRFYVLFLDDFTDFLWTFPLSNKSQVF 2
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 61.2 bits (147), Expect = 1e-09
Identities = 32/75 (42%), Positives = 46/75 (60%), Gaps = 3/75 (4%)
Frame = +1
Query: 1210 IHCDSQSAIHLANHQIYHERTKHINIRLHFVRDMIETKEIKVEKVASEENPADIFTKSLP 1269
++CD+ SA H+A + ++HERTKHI I H VR+ I+ I + ++S E ADI+TK+L
Sbjct: 58 VYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLIHLLPISSSEPLADIYTKALS 237
Query: 1270 RSRFKHC---LDLIN 1281
F L LIN
Sbjct: 238 PQNFHQICAKLGLIN 282
>AV780009
Length = 529
Score = 58.9 bits (141), Expect = 5e-09
Identities = 27/66 (40%), Positives = 41/66 (61%)
Frame = -1
Query: 1208 VKIHCDSQSAIHLANHQIYHERTKHINIRLHFVRDMIETKEIKVEKVASEENPADIFTKS 1267
V + CD+QSA+H+A++ +HERTKHI + H VR ++ I + +++ ADIFTK
Sbjct: 217 VPLFCDNQSALHIAHNPTFHERTKHIELDCHVVRAKLQAGLIHLLPISTHHQLADIFTKP 38
Query: 1268 LPRSRF 1273
P F
Sbjct: 37 CPHLCF 20
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 40.0 bits (92), Expect(2) = 7e-09
Identities = 14/36 (38%), Positives = 25/36 (68%)
Frame = +2
Query: 1210 IHCDSQSAIHLANHQIYHERTKHINIRLHFVRDMIE 1245
++CD+Q AI+L + +H R+KHI++R H+ +E
Sbjct: 173 LYCDNQGAIYLGQNSTFHSRSKHIDVRYHWTT*CVE 280
Score = 38.1 bits (87), Expect(2) = 7e-09
Identities = 14/37 (37%), Positives = 29/37 (77%)
Frame = +3
Query: 1240 VRDMIETKEIKVEKVASEENPADIFTKSLPRSRFKHC 1276
+RD++ +K +++EK+ ++++ AD+ TKSLPR + + C
Sbjct: 264 LRDVLNSKLLELEKIHTDDDGADMMTKSLPREKLEVC 374
>BP033602
Length = 533
Score = 56.6 bits (135), Expect = 2e-08
Identities = 32/92 (34%), Positives = 50/92 (53%)
Frame = +3
Query: 415 DITKLWHLRLGHVSERGLVELAKQGLLGNEKLNKLDFCDNCTLGKQHKVKFGVGVHKSSR 474
D LWH RLGH + + L L L N + L+ C++C L K + + + +SR
Sbjct: 264 DQIMLWHNRLGHPNFQYLRHLFPD-LFKNVNCSSLE-CESCVLAKNQRAPYYSQPYHASR 437
Query: 475 PFE*VHSDLLGPA*VKTYGGGSYFTSIIDDYS 506
PF +HSD+ GP+ + T G +F + IDD++
Sbjct: 438 PFYLIHSDVWGPSKITTQFGKRWFVTFIDDHT 533
>BP057233
Length = 473
Score = 55.8 bits (133), Expect = 4e-08
Identities = 27/72 (37%), Positives = 41/72 (56%)
Frame = -2
Query: 1212 CDSQSAIHLANHQIYHERTKHINIRLHFVRDMIETKEIKVEKVASEENPADIFTKSLPRS 1271
CD+ SA LA++ + H R+KHI I +H++RD + E+ V V + + AD TK L +
Sbjct: 439 CDNLSAKALASNPVLHARSKHIEIDVHYIRDQVLQNEVVVAYVPTTDQIADCLTKPLSHT 260
Query: 1272 RFKHCLDLINFI 1283
RF D + I
Sbjct: 259 RFSQLRDKLGVI 224
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 54.7 bits (130), Expect = 9e-08
Identities = 29/80 (36%), Positives = 50/80 (62%), Gaps = 1/80 (1%)
Frame = +3
Query: 1190 EAIWLKGMIGEMGI-SQGCVKIHCDSQSAIHLANHQIYHERTKHINIRLHFVRDMIETKE 1248
EA+WL + ++ I S V I+CD++SA+HLA + ++H+RT++I I H V +
Sbjct: 21 EALWLTYALADLRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDCHIV*VKVLFGI 200
Query: 1249 IKVEKVASEENPADIFTKSL 1268
+ + V S + AD+FTK++
Sbjct: 201 LHLLHVPSSDQVADVFTKTI 260
>AV778782
Length = 351
Score = 49.7 bits (117), Expect = 3e-06
Identities = 21/43 (48%), Positives = 31/43 (71%)
Frame = -3
Query: 1238 HFVRDMIETKEIKVEKVASEENPADIFTKSLPRSRFKHCLDLI 1280
H +RD++ +I VEK+ + ENPAD+ TK LP +F+ CLDL+
Sbjct: 343 HSIRDIVAAGKIIVEKIHTYENPADMLTKPLPNVKFQPCLDLV 215
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 45.4 bits (106), Expect = 6e-05
Identities = 21/61 (34%), Positives = 27/61 (43%)
Frame = +1
Query: 195 DSGEGLNVSRGRNQNRGKGKGKNSKSKSRSKGDGNKTKYKCFICHNPGHFKKDCPERKDN 254
D G V N G G G + RS G G + KC+ C PGHF ++C R +
Sbjct: 259 DGKNGWRVELSHNSRGGGGGGGGGRGGGRSGGGGGGSDMKCYECGEPGHFARECRMRGGS 438
Query: 255 G 255
G
Sbjct: 439 G 441
>TC9039
Length = 1218
Score = 45.4 bits (106), Expect = 6e-05
Identities = 27/85 (31%), Positives = 41/85 (47%), Gaps = 2/85 (2%)
Frame = +3
Query: 315 VRLGNNKACKIQGIGTIRLKMFDDRDFLLKDVRYIPELRRNLISISMFDGLGYCTRIERG 374
+ +G+ K +++ IG RL + LKD +P RRNLIS+S D GY
Sbjct: 960 IYVGDGKTVEVEAIGHFRLLLCTGFYLDLKDTFVVPSFRRNLISVSNLDKSGYSCSFGNS 1139
Query: 375 VMRISHGALVIAKGSKI--HGLYIL 397
+S + ++ GS I LY+L
Sbjct: 1140 KAELSFNSNIVGTGSLIGYDNLYLL 1214
Score = 38.1 bits (87), Expect = 0.009
Identities = 50/219 (22%), Positives = 90/219 (40%), Gaps = 7/219 (3%)
Frame = +2
Query: 37 LKREAQMSAHLTPAEKTEMNDKAVSAIILCLGDKMLREVSRETTAVSMWNKLDLL----- 91
L R++ S EK + +++ +S +I+ G + EV R T + + D L
Sbjct: 257 LLRKSSTSEQRKDYEKWDRSNR-MSLMIIKRG---IPEVFRGTISEEIKGAKDFLAEIEK 424
Query: 92 -YMTKSLAHRQCLKQQLYFYRMMESKPIMEQLTEFNKIIDDLANIDVNLEDEDKALHL-L 149
+ A L Q L + I E + + I L + + L D D +HL L
Sbjct: 425 RFAKSDKAETSTLLQNLISMKYQGKGNIREYIMGMSNIASKLKALKLELSD-DLLIHLVL 601
Query: 150 CALPRSFENFKDTMLYGKEGTITLEEVQATLITKELTKFKDLKVDDSGEGLNVSRGRNQN 209
+LP F FK + KE +L E+ + + +E ++ K + +G+ +
Sbjct: 602 LSLPAQFSQFKISYNCPKEKW-SLNELISFCVQEEERLKQERKESAHFVSTSKDKGKRKK 778
Query: 210 RGKGKGKNSKSKSRSKGDGNKTKYKCFICHNPGHFKKDC 248
+ K + + + + K + T C+ C+ GH KK C
Sbjct: 779 TVEPKNEAADAPAPKKQKEDDT---CYFCNVSGHMKKKC 886
>TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprotein, partial
(4%)
Length = 528
Score = 36.6 bits (83), Expect(2) = 2e-04
Identities = 16/54 (29%), Positives = 30/54 (54%)
Frame = +3
Query: 1230 TKHINIRLHFVRDMIETKEIKVEKVASEENPADIFTKSLPRSRFKHCLDLINFI 1283
T +I + HF+RD + +I ++ ++ ADI TK L RF++ ++N +
Sbjct: 63 TLNIETKYHFLRDQVTKGKISLKHCGTDLQVADIMTKGLKTERFRNMRAMLNVV 224
Score = 26.6 bits (57), Expect(2) = 2e-04
Identities = 11/21 (52%), Positives = 16/21 (75%)
Frame = +2
Query: 1213 DSQSAIHLANHQIYHERTKHI 1233
D++SAI LA + + H R+KHI
Sbjct: 5 DNKSAIDLAKNPVSHGRSKHI 67
>BP058111
Length = 570
Score = 42.7 bits (99), Expect = 4e-04
Identities = 25/64 (39%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = -1
Query: 419 LWHLRLGHVSERGLVELAKQ-GLLGNEKLNKLDFCDNCTLGKQHKVKFGVGVHKSSRPFE 477
LWHLRLGH S + L L K + +K+ CD C + KQ K+ F V SS F+
Sbjct: 186 LWHLRLGHTSSKKLAILQKNFPFITCKKITSP--CDTCHMAKQKKLSFPNSVTLSSEIFD 13
Query: 478 *VHS 481
+H+
Sbjct: 12 LIHT 1
>TC8302
Length = 494
Score = 42.0 bits (97), Expect = 6e-04
Identities = 37/159 (23%), Positives = 64/159 (39%), Gaps = 4/159 (2%)
Frame = +3
Query: 105 QQLYFYRMMESKPIMEQLTEFNKIIDDLANIDVNLEDEDKALHLLCALPRSFE----NFK 160
++LY RM ES + + + N + L+ + + + ++A LL +LP S++ N K
Sbjct: 3 KRLYTLRMSESTSMSDHIDHMNTLFAQLSASNFTIGENERAELLLESLPDSYDQLVINIK 182
Query: 161 DTMLYGKEGTITLEEVQATLITKELTKFKDLKVDDSGEGLNVSRGRNQNRGKGKGKNSKS 220
+ + + L EV ++ K E L ++RGR+++R
Sbjct: 183 NNNIVNHLPLMMLSEVLLEEDSQR*NKEDR*DSSKPMEALTMTRGRSKSR---------- 332
Query: 221 KSRSKGDGNKTKYKCFICHNPGHFKKDCPERKDNGGGNP 259
K KC+ C H KKDC K + P
Sbjct: 333 --------KKINLKCYHCGQR*HLKKDCWFNKKSSDVTP 425
>BP054557
Length = 538
Score = 40.4 bits (93), Expect = 0.002
Identities = 41/167 (24%), Positives = 68/167 (40%), Gaps = 17/167 (10%)
Frame = -2
Query: 288 VLDSGCSYHISPRKGYFETLELEEGGVVRLGNNKACKIQGIGTIRLKMFDDRDFLLKDVR 347
+LD+G ++H+ F+ L ++ + N + + I + ++ F L V
Sbjct: 537 LLDTGATHHVCHNLDVFQALRQVHPVLLSMPNGQ----KEIAHMADSVYFSEKFFLAXVL 370
Query: 348 YIPELRRNLISISMFDGLGYCTRI---ERGVMRISHGALVIAKGSKIHGLYIL------- 397
Y+P NLIS+S C + E +++ I + GLY +
Sbjct: 369 YVPSFNFNLISVSALVKSLNCRLVIFDECCLLQERPTLKTIGAAEERGGLYAVIDPAVKA 190
Query: 398 -----EGS--TIIAHASVPSVDTLDITKLWHLRLGHVSERGLVELAK 437
EG+ T ++H + D LWHLRLGH S + L L K
Sbjct: 189 KHPPAEGNIKTQVSHFNFCQFDC----NLWHLRLGHTSSKKLAILQK 61
>AV764934
Length = 406
Score = 39.3 bits (90), Expect = 0.004
Identities = 24/78 (30%), Positives = 35/78 (44%)
Frame = -2
Query: 410 SVDTLDITKLWHLRLGHVSERGLVELAKQGLLGNEKLNKLDFCDNCTLGKQHKVKFGVGV 469
SV WH RLGH L + + + ++F C LGK H++ +
Sbjct: 243 SVSAFVTLSTWHSRLGHPHLDNLKRVLSSCNVSYSPKDTVEFRTACCLGKAHRLPSQMST 64
Query: 470 HKSSRPFE*VHSDLLGPA 487
+ PFE ++SDL GPA
Sbjct: 63 -TTYLPFELIYSDLWGPA 13
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.343 0.153 0.518
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,236,096
Number of Sequences: 28460
Number of extensions: 312612
Number of successful extensions: 2884
Number of sequences better than 10.0: 67
Number of HSP's better than 10.0 without gapping: 2781
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2855
length of query: 1283
length of database: 4,897,600
effective HSP length: 101
effective length of query: 1182
effective length of database: 2,023,140
effective search space: 2391351480
effective search space used: 2391351480
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.5 bits)
S2: 61 (28.1 bits)
Medicago: description of AC135504.5