BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135504.2 - phase: 0 /pseudo
(2338 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP065719 65 2e-10
TC18149 weakly similar to UP|Q91BL3 (Q91BL3) Essential structura... 39 0.009
TC15253 weakly similar to UP|Q9ZT16 (Q9ZT16) Arabinogalactan-pro... 36 0.079
TC18683 weakly similar to UP|Q8H599 (Q8H599) OJ1634_B10.18 prote... 35 0.10
TC9594 weakly similar to GB|AAM19966.1|20466089|AY098956 At2g326... 34 0.30
AV407673 33 0.39
AV422434 33 0.51
TC12500 similar to GB|AAH45354.1|28279536|BC045354 FNBP4 protein... 33 0.67
AU251550 32 0.87
TC8445 similar to UP|O80910 (O80910) At2g38410 protein, partial ... 32 0.87
BG662122 32 1.1
TC14737 similar to UP|Q9LEB3 (Q9LEB3) RNA binding protein 47, pa... 32 1.1
TC14162 similar to UP|OLP1_LYCES (Q41350) Osmotin-like protein p... 32 1.1
TC15341 similar to UP|Q9M0H8 (Q9M0H8) Predicted proline-rich pro... 32 1.1
TC17113 weakly similar to UP|Q9LFU8 (Q9LFU8) Proline-rich protei... 32 1.1
AV427333 32 1.5
AV422734 32 1.5
BI418387 32 1.5
TC16093 weakly similar to UP|PF21_ARATH (Q04088) Possible transc... 32 1.5
TC15540 similar to UP|Q9NGS5 (Q9NGS5) Prespore protein MF12 (Fra... 31 2.5
>BP065719
Length = 567
Score = 64.7 bits (156), Expect = 2e-10
Identities = 38/127 (29%), Positives = 69/127 (53%), Gaps = 2/127 (1%)
Frame = +3
Query: 166 DIPKKFKVPEFDRYNGLTCPQN--HIIKYVRKMGNYSDNDSLMIHCFQDSLMEDAAEWYT 223
++P+ +KVP+F +++G + HI +Y + G+ + N++L + F SL ++A W+T
Sbjct: 159 ELPRGWKVPKFTKFSGDSGESTVEHIARYQIEAGDLAINENLKMKYFPSSLTKNAFTWFT 338
Query: 224 SLSKDDVHTFDELAAAFKNHYGFNTRLKPSREFLRSLSQKKDESFCEYAQRWRGAAARIT 283
+L+ VHT+ +L F + F K S + L S+ +K ES +Y R+R +R
Sbjct: 339 TLAPRSVHTWAQLERIFHEQF-FRGECKVSXKDLASVKRKPAESIDDYLNRFRMLKSRCF 515
Query: 284 PALDEEE 290
+ E E
Sbjct: 516 THVSEHE 536
>TC18149 weakly similar to UP|Q91BL3 (Q91BL3) Essential structural protein
pp78/81, partial (5%)
Length = 710
Score = 38.9 bits (89), Expect = 0.009
Identities = 43/134 (32%), Positives = 50/134 (37%), Gaps = 25/134 (18%)
Frame = +3
Query: 370 QSMATVAPINAA-QLPPLY-------PYAQYSQ---------HPFFPPFYHQYPLPSGQP 412
QS+ V P NA QLPP P Q+SQ P+FPP P+ Q
Sbjct: 99 QSIPVVPPPNAPPQLPPQQGLPPSFPPPNQFSQTQIPAVPQRDPYFPPPVQSQETPNQQY 278
Query: 413 QVPVNAIVQQMQQQPPTQQQQQ-------QQARPTF-PPIPMLYFELLPTLLLRGHCTTR 464
Q P+ PP QQ QQ Q A P PPIP + L + L GH
Sbjct: 279 QQPLAPQPHPQPGAPPHQQYQQTPHPQYSQPAPPQHQPPIPSINPPQLQSSL--GHHVEE 452
Query: 465 QGKPPPDPLPPRFR 478
P PP R
Sbjct: 453 PPYVPSQTYPPNLR 494
>TC15253 weakly similar to UP|Q9ZT16 (Q9ZT16) Arabinogalactan-protein
(ATAGP4) (AT5G10430/F12B17_220), partial (42%)
Length = 575
Score = 35.8 bits (81), Expect = 0.079
Identities = 28/100 (28%), Positives = 39/100 (39%), Gaps = 2/100 (2%)
Frame = +1
Query: 347 KRNGNGHHKKKETEVWMVSAGAGQSMATVAPINAAQLPPLYPYA--QYSQHPFFPPFYHQ 404
K HH +++ + + Q + + P+ QLPPL P Q P PP +
Sbjct: 172 KHRQQPHHHRQQQHLPHLQQLHHQQLPLLPPL-LHQLPPLLPPPPLQLPLQPLPPPVHPP 348
Query: 405 YPLPSGQPQVPVNAIVQQMQQQPPTQQQQQQQARPTFPPI 444
PLP PQ + Q Q PP Q Q PP+
Sbjct: 349 LPLPLHPPQ---SHPAQHQAQAPPLDQAQTALIHHHLPPL 459
>TC18683 weakly similar to UP|Q8H599 (Q8H599) OJ1634_B10.18 protein, partial
(37%)
Length = 715
Score = 35.4 bits (80), Expect = 0.10
Identities = 28/99 (28%), Positives = 39/99 (39%), Gaps = 16/99 (16%)
Frame = -3
Query: 365 SAGAGQSMATVAPINAAQLPPLYPYAQYSQHPFFPPF----------------YHQYPLP 408
+A + T+ P A LPPL + + P PPF + PLP
Sbjct: 590 TAAPALGLPTLPPFPKATLPPLPSQPSFPK-PTLPPFPAMPKFTLPPLPTMPKFTLPPLP 414
Query: 409 SGQPQVPVNAIVQQMQQQPPTQQQQQQQARPTFPPIPML 447
S QPQ+P N + PT + + PT P +P L
Sbjct: 413 SSQPQLP-NTLPPLPTFPLPTAPKLTLPSLPTLPTVPFL 300
>TC9594 weakly similar to GB|AAM19966.1|20466089|AY098956
At2g32600/T26B15.16 {Arabidopsis thaliana;}, partial
(9%)
Length = 502
Score = 33.9 bits (76), Expect = 0.30
Identities = 29/99 (29%), Positives = 35/99 (35%), Gaps = 8/99 (8%)
Frame = +3
Query: 384 PPLYPYAQYSQHPFFPPFYHQYPLPSGQP----QVPVNAIV----QQMQQQPPTQQQQQQ 435
PP P QY Q P P P G P +P A++ Q+ + PP Q QQQ
Sbjct: 69 PPTMPPPQYQQFRPPPSGMQMPPPPQGVPGPPRHLPPPAVMGGQPPQVWRPPPPPQYQQQ 248
Query: 436 QARPTFPPIPMLYFELLPTLLLRGHCTTRQGKPPPDPLP 474
RP P + PPP PLP
Sbjct: 249 VGRPMAYP--------------------QSSMPPPPPLP 305
>AV407673
Length = 427
Score = 33.5 bits (75), Expect = 0.39
Identities = 12/21 (57%), Positives = 15/21 (71%)
Frame = -3
Query: 385 PLYPYAQYSQHPFFPPFYHQY 405
PL+P Q +HPFFPPF+ Y
Sbjct: 197 PLFPTFQNYRHPFFPPFFFSY 135
>AV422434
Length = 422
Score = 33.1 bits (74), Expect = 0.51
Identities = 14/35 (40%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = +2
Query: 442 PPIPMLYFELLPTLLLRGHCTTRQGKP-PPDPLPP 475
PP+P+ F + P L+R H + +P P PLPP
Sbjct: 125 PPLPLRLFHMAPLFLIRRHAPSPLSQPSPTTPLPP 229
>TC12500 similar to GB|AAH45354.1|28279536|BC045354 FNBP4 protein {Danio
rerio;} , partial (5%)
Length = 678
Score = 32.7 bits (73), Expect = 0.67
Identities = 30/100 (30%), Positives = 37/100 (37%), Gaps = 8/100 (8%)
Frame = +1
Query: 384 PPLYPYAQYSQHPFFPPFYHQYPLPSGQPQVPVNAIVQQ---MQQQPPTQQQQQQQARPT 440
PPL A + P PP H L + P I Q QPP+ + A
Sbjct: 367 PPLLMSAT-DRAPLPPPSTHLTSLTTPSPPSSRTTITQTPLPSPPQPPSPPPKYGVATIP 543
Query: 441 FPPIPMLYFELLPTLLLRGHCTTRQGKPP-----PDPLPP 475
PP+PM + P LL T+ PP P P PP
Sbjct: 544 PPPLPMSSIDRTPLPLLSTPLTSLDAPPPTPTLSPPPPPP 663
>AU251550
Length = 329
Score = 32.3 bits (72), Expect = 0.87
Identities = 23/63 (36%), Positives = 26/63 (40%), Gaps = 1/63 (1%)
Frame = +2
Query: 384 PPLYPYAQYSQHPFFPPFYHQY-PLPSGQPQVPVNAIVQQMQQQPPTQQQQQQQARPTFP 442
PP P Y+ P PPF H + P P G P P A M QPP +P P
Sbjct: 140 PPGPPRDPYAPAPPPPPFGHHHPPPPHGPPSPPPPA----MFYQPPPPPGPPGPPQPPHP 307
Query: 443 PIP 445
P P
Sbjct: 308 PGP 316
>TC8445 similar to UP|O80910 (O80910) At2g38410 protein, partial (7%)
Length = 841
Score = 32.3 bits (72), Expect = 0.87
Identities = 21/49 (42%), Positives = 25/49 (50%)
Frame = +1
Query: 391 QYSQHPFFPPFYHQYPLPSGQPQVPVNAIVQQMQQQPPTQQQQQQQARP 439
Q HP F P H P QPQ P + +QQ Q QP +Q Q QQ +P
Sbjct: 67 QPQHHPHFQP--HPQPQLQSQPQ-PQH--IQQSQPQPQSQPQHIQQPQP 198
>BG662122
Length = 386
Score = 32.0 bits (71), Expect = 1.1
Identities = 18/56 (32%), Positives = 30/56 (53%), Gaps = 2/56 (3%)
Frame = +2
Query: 460 HCTTRQGKPPPDPLPPRFRSDLK--CDFHQGALGHDVEGCYALKYIVKKLIDQGKL 513
H + G+ P PPR D C++ + + HD++ C+ LK ++KLI G+L
Sbjct: 146 HMVGKSGQS*P---PPRRGIDTTK*CEYRRSVV-HDIDDCFTLKREIEKLIKMGRL 301
>TC14737 similar to UP|Q9LEB3 (Q9LEB3) RNA binding protein 47, partial (32%)
Length = 803
Score = 32.0 bits (71), Expect = 1.1
Identities = 30/106 (28%), Positives = 44/106 (41%), Gaps = 5/106 (4%)
Frame = +3
Query: 336 FEKVESSANASKRNGNGHHKKKETEVWMVSAGAGQSMATVAPINAAQLPPLYPYAQYSQH 395
F + S ++ N N ++++ + W +A Q MA P A + Y QH
Sbjct: 117 FTMAQPSNGSADLNQNAPPQQQQPQQW--AAQQQQWMAMQYPATAMAMMQ-QQMMMYPQH 287
Query: 396 PFFPPFYHQYPLPSGQPQVPVNAIV-----QQMQQQPPTQQQQQQQ 436
+ P+ H + P Q Q A QQ QQ QQQ+QQQ
Sbjct: 288 --YMPYVHHHYQPHHQQQQAAAAAAGAVPQQQHQQHHHQQQQKQQQ 419
>TC14162 similar to UP|OLP1_LYCES (Q41350) Osmotin-like protein precursor,
partial (87%)
Length = 1232
Score = 32.0 bits (71), Expect = 1.1
Identities = 13/51 (25%), Positives = 30/51 (58%), Gaps = 1/51 (1%)
Frame = +3
Query: 395 HPFFPPFYHQYPLPSGQPQVPVNAIVQQ-MQQQPPTQQQQQQQARPTFPPI 444
H PP PLP+ + Q+ ++ + + Q++PP+ ++++ + +P PP+
Sbjct: 75 HHHHPPHTFTCPLPNPRQQLQIHRLARHPAQRRPPSPRRRRLRPQPLHPPL 227
>TC15341 similar to UP|Q9M0H8 (Q9M0H8) Predicted proline-rich protein,
partial (33%)
Length = 1002
Score = 32.0 bits (71), Expect = 1.1
Identities = 32/103 (31%), Positives = 44/103 (42%), Gaps = 14/103 (13%)
Frame = +1
Query: 390 AQYSQHPFFPPFYHQYPLPSGQ------PQVPVNA--------IVQQMQQQPPTQQQQQQ 435
AQ Q+ + PP QY P Q PQ N + Q Q Q P QQQQQQ
Sbjct: 709 AQLPQNQYLPPD-QQYRTPQMQDMSRVAPQPCTNXSGXSPPPPVQQYPQYQQPQQQQQQQ 885
Query: 436 QARPTFPPIPMLYFELLPTLLLRGHCTTRQGKPPPDPLPPRFR 478
Q + +P L ++ PT + Q +PP P+ P ++
Sbjct: 886 QQQQQWP--QQLPQQVQPT--QPPSMQSPQIRPPSSPVYPPYQ 1002
>TC17113 weakly similar to UP|Q9LFU8 (Q9LFU8) Proline-rich protein, partial
(25%)
Length = 1077
Score = 32.0 bits (71), Expect = 1.1
Identities = 32/104 (30%), Positives = 39/104 (36%), Gaps = 3/104 (2%)
Frame = +1
Query: 377 PINAAQLPPLYP--YAQYSQHPFFP-PFYHQYPLPSGQPQVPVNAIVQQMQQQPPTQQQQ 433
P PPL+P + S P P PF P PS P +P N +QPP+
Sbjct: 547 PFQPPSPPPLFPNPFQPPSPPPLIPNPFQ---PPPSPPPFIP-NPFQPPPSKQPPS---- 702
Query: 434 QQQARPTFPPIPMLYFELLPTLLLRGHCTTRQGKPPPDPLPPRF 477
P FP P++ L P PPP P PP F
Sbjct: 703 -----PLFPFPPIVIPGLTP--------------PPPPPPPPPF 777
>AV427333
Length = 387
Score = 31.6 bits (70), Expect = 1.5
Identities = 27/94 (28%), Positives = 34/94 (35%), Gaps = 2/94 (2%)
Frame = +3
Query: 384 PPLYPYAQYS--QHPFFPPFYHQYPLPSGQPQVPVNAIVQQMQQQPPTQQQQQQQARPTF 441
PP +PY + HPF PP + S P A + PP + P
Sbjct: 87 PPSHPYNPPTPPSHPFNPPPTPSHYFKSPPPPSHSFAPPPRGHPSPPPSSPPPPSSHPFT 266
Query: 442 PPIPMLYFELLPTLLLRGHCTTRQGKPPPDPLPP 475
PP P + P + R PPP PLPP
Sbjct: 267 PPPPHVRPPPSPHHPITPPPHVR---PPPPPLPP 359
>AV422734
Length = 448
Score = 31.6 bits (70), Expect = 1.5
Identities = 14/22 (63%), Positives = 15/22 (67%)
Frame = +1
Query: 421 QQMQQQPPTQQQQQQQARPTFP 442
QQ QQQP QQQQQ+ RP P
Sbjct: 373 QQQQQQPQQQQQQQKMVRPGNP 438
>BI418387
Length = 479
Score = 31.6 bits (70), Expect = 1.5
Identities = 21/75 (28%), Positives = 32/75 (42%), Gaps = 6/75 (8%)
Frame = +3
Query: 377 PINAAQLPPLYPYAQYSQHPFFPPFYHQYPLPSGQPQVP------VNAIVQQMQQQPPTQ 430
P+ PP + + Q P +P ++PLP QP P +VQ+ P
Sbjct: 228 PLPLLAFPPFHSGL*FLQPPHWPHPTRRHPLPRPQPNHPRPLPRSHRFLVQRAHSLRPQD 407
Query: 431 QQQQQQARPTFPPIP 445
Q+ +Q P PP+P
Sbjct: 408 QKIRQAQNP--PPLP 446
>TC16093 weakly similar to UP|PF21_ARATH (Q04088) Possible transcription
factor PosF21 (AtbZIP59), partial (17%)
Length = 570
Score = 31.6 bits (70), Expect = 1.5
Identities = 29/81 (35%), Positives = 35/81 (42%), Gaps = 8/81 (9%)
Frame = +1
Query: 365 SAGAGQ-------SMATVAPINAAQLPPLYPYA-QYSQHPFFPPFYHQYPLPSGQPQVPV 416
S G GQ SM T+ Q ++P Q QH F P Q Q Q P+
Sbjct: 22 SFGGGQQFYPHNHSMHTLLAAQQFQQLQIHPQKHQQHQHQFQQPIQQQQQ----QQQQPI 189
Query: 417 NAIVQQMQQQPPTQQQQQQQA 437
QQMQQQ QQ+Q QQ+
Sbjct: 190 QQPQQQMQQQ---QQEQHQQS 243
Score = 29.6 bits (65), Expect = 5.6
Identities = 21/65 (32%), Positives = 30/65 (45%), Gaps = 6/65 (9%)
Frame = +1
Query: 385 PLYPYAQYSQ-HPFFPPFYHQYPLPSGQPQVPVNAIVQQMQQ-----QPPTQQQQQQQAR 438
P+ YA + F+P + + L + Q + Q+ QQ Q P QQQQQQQ +
Sbjct: 4 PMMNYASFGGGQQFYPHNHSMHTLLAAQQFQQLQIHPQKHQQHQHQFQQPIQQQQQQQQQ 183
Query: 439 PTFPP 443
P P
Sbjct: 184 PIQQP 198
>TC15540 similar to UP|Q9NGS5 (Q9NGS5) Prespore protein MF12 (Fragment),
partial (6%)
Length = 806
Score = 30.8 bits (68), Expect = 2.5
Identities = 27/77 (35%), Positives = 31/77 (40%), Gaps = 5/77 (6%)
Frame = +2
Query: 382 QLPPLYPYAQYSQHPFFPPFYHQYPLPSGQ--PQVPVNAIVQQMQQQPPTQQQQ---QQQ 436
QLP L Q Q P P Q Q PQV + Q QQQ P QQQ Q Q
Sbjct: 5 QLPQLQQQQQLQQQP---PQLTQLQQQQQQQLPQVQQQQLSQMQQQQLPQLQQQQLSQLQ 175
Query: 437 ARPTFPPIPMLYFELLP 453
+ P + L + LP
Sbjct: 176 PQQQLPQLQQLQHQQLP 226
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.340 0.147 0.493
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,437,557
Number of Sequences: 28460
Number of extensions: 651421
Number of successful extensions: 5809
Number of sequences better than 10.0: 69
Number of HSP's better than 10.0 without gapping: 5297
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5677
length of query: 2338
length of database: 4,897,600
effective HSP length: 105
effective length of query: 2233
effective length of database: 1,909,300
effective search space: 4263466900
effective search space used: 4263466900
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.9 bits)
S2: 63 (28.9 bits)
Medicago: description of AC135504.2