BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135464.2 - phase: 0 /pseudo
(1353 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV780009 160 1e-39
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 65 7e-11
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 60 2e-09
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 55 1e-07
AV766665 51 1e-06
BP084332 47 3e-05
BP043850 44 2e-04
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 36 3e-04
TC10484 similar to UP|Q850H7 (Q850H7) Gag-pol polyprotein (Fragm... 33 3e-04
BI418295 40 0.002
BP057233 39 0.006
BP084259 37 0.016
TC19474 weakly similar to UP|Q9FJA1 (Q9FJA1) Similarity to retro... 36 0.036
TC14279 similar to UP|Q09085 (Q09085) Hydroxyproline-rich glycop... 36 0.047
BP052124 34 0.18
BI417786 34 0.18
AV779990 33 0.23
TC16065 weakly similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich... 33 0.30
TC8768 homologue to UP|Q9SMJ5 (Q9SMJ5) DTDP-glucose 4-6-dehydrat... 33 0.40
AU088980 31 1.5
>AV780009
Length = 529
Score = 160 bits (406), Expect = 1e-39
Identities = 76/122 (62%), Positives = 90/122 (73%)
Frame = -1
Query: 1180 SPGTGLFFPRDSSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSS 1239
+P GLFF DS L L YSD+DW GCPDTRRS+TGY F+G SL+SW++KKQ VSRSS
Sbjct: 493 APAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISWRTKKQTTVSRSS 314
Query: 1240 SEAEYRALVASTCELQ*LVYLLTDLNVTCSKVPGLLCDSQSALHIASNPIFHERTKHLDI 1299
SEAEYRAL A+ CE+Q L YL L + L CD+QSALHIA NP FHERTKH+++
Sbjct: 313 SEAEYRALAATVCEVQWLSYLFQFLKLNVPLPVPLFCDNQSALHIAHNPTFHERTKHIEL 134
Query: 1300 DC 1301
DC
Sbjct: 133 DC 128
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 65.1 bits (157), Expect = 7e-11
Identities = 32/50 (64%), Positives = 35/50 (70%)
Frame = +1
Query: 1252 CELQ*LVYLLTDLNVTCSKVPGLLCDSQSALHIASNPIFHERTKHLDIDC 1301
CEL L Y+L DL V L CDSQSA HIA+N +FHERTKHLDIDC
Sbjct: 1 CEL*WLTYILQDLRVPFISPSLLYCDSQSARHIATNAVFHERTKHLDIDC 150
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 60.1 bits (144), Expect = 2e-09
Identities = 27/47 (57%), Positives = 34/47 (71%)
Frame = +1
Query: 1255 Q*LVYLLTDLNVTCSKVPGLLCDSQSALHIASNPIFHERTKHLDIDC 1301
Q L YLL DL V + + CD+ SA HIA+NP+FHERTKH++IDC
Sbjct: 1 QWLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDC 141
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 54.7 bits (130), Expect = 1e-07
Identities = 22/54 (40%), Positives = 37/54 (67%)
Frame = +3
Query: 1248 VASTCELQ*LVYLLTDLNVTCSKVPGLLCDSQSALHIASNPIFHERTKHLDIDC 1301
++ CE L Y L DL + + + CD++SALH+A+N +FH+RT++++IDC
Sbjct: 6 ISHPCEALWLTYALADLRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDC 167
>AV766665
Length = 601
Score = 51.2 bits (121), Expect = 1e-06
Identities = 26/63 (41%), Positives = 39/63 (61%)
Frame = +3
Query: 1184 GLFFPRDSSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAE 1243
G F ++ + G++ AD+ G R S GY F+ +LV+W+SK+Q I++RSS EAE
Sbjct: 408 GPLFQKEGKSSMDGFTYADYLGSIVDRLSTMGYYMFLSGNLVTWRSKQQNIIARSSGEAE 587
Query: 1244 YRA 1246
RA
Sbjct: 588 LRA 596
>BP084332
Length = 368
Score = 46.6 bits (109), Expect = 3e-05
Identities = 20/68 (29%), Positives = 41/68 (59%)
Frame = +2
Query: 1232 QQIVSRSSSEAEYRALVASTCELQ*LVYLLTDLNVTCSKVPGLLCDSQSALHIASNPIFH 1291
Q ++ S++EAEY + + ++ + + L D + S +P + CD+ +A+ ++ NPI H
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIP-IYCDNTAAISLSKNPILH 208
Query: 1292 ERTKHLDI 1299
R KH+++
Sbjct: 209 SRAKHIEV 232
>BP043850
Length = 515
Score = 43.9 bits (102), Expect = 2e-04
Identities = 18/42 (42%), Positives = 30/42 (70%)
Frame = -1
Query: 1260 LLTDLNVTCSKVPGLLCDSQSALHIASNPIFHERTKHLDIDC 1301
LL++L S+ L D+ SA+ IA+NP++HE T+H+++DC
Sbjct: 491 LLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDC 366
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 36.2 bits (82), Expect(2) = 3e-04
Identities = 12/26 (46%), Positives = 19/26 (72%)
Frame = +2
Query: 1274 LLCDSQSALHIASNPIFHERTKHLDI 1299
L CD+Q A+++ N FH R+KH+D+
Sbjct: 173 LYCDNQGAIYLGQNSTFHSRSKHIDV 250
Score = 25.8 bits (55), Expect(2) = 3e-04
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = +3
Query: 1225 VSWKSKKQQIVSRSSSEAEYRALVASTCEL 1254
V+W S+ Q+ V+ S++EAE+ A + EL
Sbjct: 27 VAWPSRLQKCVALSTAEAEFIAATEACHEL 116
>TC10484 similar to UP|Q850H7 (Q850H7) Gag-pol polyprotein (Fragment), partial
(20%)
Length = 479
Score = 32.7 bits (73), Expect(2) = 3e-04
Identities = 16/43 (37%), Positives = 23/43 (53%)
Frame = +2
Query: 1284 IASNPIFHERTKHLDIDCQCSEGETSKWTHGIVSYL*SRADCR 1326
I S HERTKH+++DC + ETS ++ L R C+
Sbjct: 92 IQSQFSLHERTKHIEVDCHSTS*ETSTEPLSLLLMLERRLTCQ 220
Score = 29.3 bits (64), Expect(2) = 3e-04
Identities = 12/29 (41%), Positives = 20/29 (68%)
Frame = +3
Query: 1260 LLTDLNVTCSKVPGLLCDSQSALHIASNP 1288
+L+++ + L CD+Q+ALHI+SNP
Sbjct: 15 ILSEMGIERISPMPLWCDNQAALHISSNP 101
>BI418295
Length = 523
Score = 40.0 bits (92), Expect = 0.002
Identities = 17/74 (22%), Positives = 41/74 (54%)
Frame = +2
Query: 1 IRCNNLVHSWLMNSVTPSITQSLIYTDSAVDAWNDLQARFSRADRVRISTLQRDLHSFRQ 60
I+ +++V +WL+ +++P + ++I + + W ++ F R + + L+ +L + +
Sbjct: 149 IKQDSMVFTWLLTTLSPFVLPTVINCERSSQVWREILDFFENQCRAQSAKLRSELKNITK 328
Query: 61 NSLSIIEYFTELKS 74
S SI EY +K+
Sbjct: 329 GSKSITEYLRRIKT 370
>BP057233
Length = 473
Score = 38.9 bits (89), Expect = 0.006
Identities = 16/29 (55%), Positives = 22/29 (75%)
Frame = -2
Query: 1272 PGLLCDSQSALHIASNPIFHERTKHLDID 1300
P L CD+ SA +ASNP+ H R+KH++ID
Sbjct: 451 PILWCDNLSAKALASNPVLHARSKHIEID 365
>BP084259
Length = 385
Score = 37.4 bits (85), Expect = 0.016
Identities = 17/57 (29%), Positives = 31/57 (53%)
Frame = -3
Query: 1245 RALVASTCELQ*LVYLLTDLNVTCSKVPGLLCDSQSALHIASNPIFHERTKHLDIDC 1301
R + ++ E L LL +L + KVP + ++ +A ++ N +FH KH+ +DC
Sbjct: 179 RTIGSTAAEFLWLQQLLKELQFSLPKVPCIFSNNINATYVCLNLVFHSHMKHVALDC 9
>TC19474 weakly similar to UP|Q9FJA1 (Q9FJA1) Similarity to retroelement pol
polyprotein, partial (3%)
Length = 517
Score = 36.2 bits (82), Expect = 0.036
Identities = 15/29 (51%), Positives = 23/29 (78%)
Frame = -1
Query: 1223 SLVSWKSKKQQIVSRSSSEAEYRALVAST 1251
+L+SWKSK+ IV+RS +EAE+R + +T
Sbjct: 508 NLISWKSKETIIVARSRAEAEFRVMATAT 422
>TC14279 similar to UP|Q09085 (Q09085) Hydroxyproline-rich glycoprotein
(HRGP) (Fragment), partial (57%)
Length = 941
Score = 35.8 bits (81), Expect = 0.047
Identities = 41/133 (30%), Positives = 55/133 (40%), Gaps = 14/133 (10%)
Frame = +2
Query: 723 HTNLYLLLNLAGSTSHFPSHHIPIHHYQHS*PHL--LLTYQPQITHHTLLHLHLSL---- 776
H + +L L +T++ P HH +HH + HL + P +T L HLHL L
Sbjct: 95 HLHHHLPLLPPHTTTNLPHHHHILHHLPTTTNHLHHHPRHLPLLTITNLPHLHLHLHRLH 274
Query: 777 ----TLTLPLNQLCLPP*ENPPDQKDYPSILWTINVTKLFIRHLIPLLTL----LAIPIF 828
L L L LP NP + + L TI L HL+P T L +P
Sbjct: 275 TTTKVLLHHLPFLILPTTTNPLHHQRHLLHLLTIM*VLLHPHHLLPHHTTMHHHLLLPHL 454
Query: 829 HLFSTSTHFLSSQ 841
L T T+ L Q
Sbjct: 455 QLPHTFTNHLPHQ 493
>BP052124
Length = 467
Score = 33.9 bits (76), Expect = 0.18
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = -2
Query: 1276 CDSQSALHIASNPIFHERTKHLDIDC 1301
CD+ SAL +A PI H R H ++DC
Sbjct: 451 CDNNSALTLAPRPI*HSRPVHFEVDC 374
>BI417786
Length = 513
Score = 33.9 bits (76), Expect = 0.18
Identities = 19/53 (35%), Positives = 23/53 (42%)
Frame = +2
Query: 723 HTNLYLLLNLAGSTSHFPSHHIPIHHYQHS*PHLLLTYQPQITHHTLLHLHLS 775
H + L+ TS+ PSHH HH+ H HH LLHL LS
Sbjct: 56 HNR*WRFCTLSHLTSYHPSHHHHHHHHHHH------------HHHVLLHLSLS 178
>AV779990
Length = 542
Score = 33.5 bits (75), Expect = 0.23
Identities = 14/20 (70%), Positives = 17/20 (85%)
Frame = +3
Query: 1277 DSQSALHIASNPIFHERTKH 1296
D+ +AL I+SNPI HERTKH
Sbjct: 312 DNHAALDISSNPISHERTKH 371
>TC16065 weakly similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich
glycoprotein DZ-HRGP precursor, partial (20%)
Length = 608
Score = 33.1 bits (74), Expect = 0.30
Identities = 18/45 (40%), Positives = 24/45 (53%)
Frame = +2
Query: 740 PSHHIPIHHYQHS*PHLLLTYQPQITHHTLLHLHLSLTLTLPLNQ 784
P HH P HH+ HS H+ L + HH L L + + L LP+ Q
Sbjct: 263 PHHHNPAHHHHHSRRHVHLV----VDHHNLYPLGVPV-LLLPVQQ 382
>TC8768 homologue to UP|Q9SMJ5 (Q9SMJ5) DTDP-glucose 4-6-dehydratase ,
partial (43%)
Length = 562
Score = 32.7 bits (73), Expect = 0.40
Identities = 16/54 (29%), Positives = 27/54 (49%)
Frame = -1
Query: 57 SFRQNSLSIIEYFTELKSYWEELEVFCPIPNCTCNVRCSCEAMRNSRRGSDTPL 110
S QNS ++ E + W+ + C + +RC C+++RNS R S T +
Sbjct: 232 SSNQNSHIGLKELGETQGRWQRRLLSCFLMVSIRRIRCHCQSLRNSNRDSSTEI 71
>AU088980
Length = 360
Score = 30.8 bits (68), Expect = 1.5
Identities = 27/76 (35%), Positives = 34/76 (44%)
Frame = +3
Query: 617 ICLPSIGGLWSLMLFLL*IKYLLMLFKEKFLINLCTMNCLICLVSKYLFHSALLLLKIVI 676
ICL G L ML + I Y + LIN M L+ K+L +LLL +
Sbjct: 69 ICLKICGLLLLNMLPI*SIXYHHRF*RASALINSLIMTLLLY*T*KFLVPFVILLLLLTX 248
Query: 677 NLNWTIELANVFFSVL 692
N +E ANVF VL
Sbjct: 249 GRNLILEPANVFSLVL 296
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.360 0.159 0.618
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,320,351
Number of Sequences: 28460
Number of extensions: 537350
Number of successful extensions: 7247
Number of sequences better than 10.0: 68
Number of HSP's better than 10.0 without gapping: 5247
Number of HSP's successfully gapped in prelim test: 245
Number of HSP's that attempted gapping in prelim test: 1623
Number of HSP's gapped (non-prelim): 5952
length of query: 1353
length of database: 4,897,600
effective HSP length: 101
effective length of query: 1252
effective length of database: 2,023,140
effective search space: 2532971280
effective search space used: 2532971280
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.9 bits)
S2: 61 (28.1 bits)
Medicago: description of AC135464.2