BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135464.1 - phase: 0 /pseudo
(317 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8302 52 1e-07
TC9039 51 3e-07
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 42 2e-04
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 39 9e-04
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 38 0.003
AV420911 35 0.013
BP065763 34 0.028
AV773507 34 0.037
TC18927 similar to PIR|AI2934|AI2934 chromate transport protein ... 33 0.049
TC9435 similar to UP|Q9AXJ7 (Q9AXJ7) Phosphatase-like protein Mt... 27 0.20
TC10378 similar to UP|Q84TG2 (Q84TG2) At3g16350, partial (26%) 31 0.24
AV421607 31 0.31
TC12416 similar to GB|AAH56066.1|33417132|BC056066 sop-prov prot... 30 0.41
TC12851 UP|Q84TG9 (Q84TG9) Beta-tubulin, partial (34%) 29 0.92
BP049555 29 1.2
BI418821 29 1.2
TC12773 28 2.0
TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, par... 28 2.7
TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, part... 28 2.7
BU494286 27 4.5
>TC8302
Length = 494
Score = 52.0 bits (123), Expect = 1e-07
Identities = 46/166 (27%), Positives = 73/166 (43%), Gaps = 6/166 (3%)
Frame = +3
Query: 105 QQLYSFKMMESKSISEQLTEFNKILDDLANIEVNMEDEDKALLLLCSLPKSFE----HFK 160
++LY+ +M ES S+S+ + N + L+ + + ++A LLL SLP S++ + K
Sbjct: 3 KRLYTLRMSESTSMSDHIDHMNTLFAQLSASNFTIGENERAELLLESLPDSYDQLVINIK 182
Query: 161 DTILYGKEGTATLEEVQAALRTKELTKFKDMKVDEGSEGLNVTRGRNEHRGKGKGKSRSK 220
+ + L EV ++ K + E L +TRG RSK
Sbjct: 183 NNNIVNHLPLMMLSEVLLEEDSQR*NKEDR*DSSKPMEALTMTRG------------RSK 326
Query: 221 SRSKGFDKSKYKCFLCHKQGHFKKDC--PDKGGDGSPSVQVAEASN 264
SR K KC+ C ++ H KKDC K D +P + SN
Sbjct: 327 SRK----KINLKCYHCGQR*HLKKDCWFNKKSSDVTPRFRWRHFSN 452
>TC9039
Length = 1218
Score = 50.8 bits (120), Expect = 3e-07
Identities = 43/173 (24%), Positives = 69/173 (39%), Gaps = 7/173 (4%)
Frame = +2
Query: 81 AASMLAKWESLYMTKSLAHRQLLKQQLYSFKMMESKSISEQLTEFNKILDDLANIEVNME 140
A LA+ E + A L Q L S K +I E + + I L +++ +
Sbjct: 395 AKDFLAEIEKRFAKSDKAETSTLLQNLISMKYQGKGNIREYIMGMSNIASKLKALKLELS 574
Query: 141 DEDKALLLLCSLPKSFEHFKDTILYGKEGTATLEEVQAALRTKELTKFKDMKVDEGSEGL 200
D+ L+L SLP F FK + KE + E + ++ +E K E E
Sbjct: 575 DDLLIHLVLLSLPAQFSQFKISYNCPKEKWSLNELISFCVQEEERLK------QERKESA 736
Query: 201 NVTRGRNEHRGKGKGKSRSKSRSKGFD-------KSKYKCFLCHKQGHFKKDC 246
+ + + KGK K + +++ D K C+ C+ GH KK C
Sbjct: 737 HFV---STSKDKGKRKKTVEPKNEAADAPAPKKQKEDDTCYFCNVSGHMKKKC 886
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 41.6 bits (96), Expect = 2e-04
Identities = 21/71 (29%), Positives = 33/71 (45%)
Frame = +1
Query: 181 RTKELTKFKDMKVDEGSEGLNVTRGRNEHRGKGKGKSRSKSRSKGFDKSKYKCFLCHKQG 240
R +E + ++ +G N R H KG G R R +G + KC+ C + G
Sbjct: 16 RERERERERERDAIHALDGKNGWRVELSHNSKGGGGGRGGGRGRGGED--LKCYECGEPG 189
Query: 241 HFKKDCPDKGG 251
HF ++C +GG
Sbjct: 190 HFARECRSRGG 222
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 39.3 bits (90), Expect = 9e-04
Identities = 17/49 (34%), Positives = 27/49 (54%), Gaps = 4/49 (8%)
Frame = +2
Query: 206 RNEHRGKGKGKSRSKSRSKGFDKSKY----KCFLCHKQGHFKKDCPDKG 250
R RG+ + + RS+G+ S+ +CF C K GHF ++CP +G
Sbjct: 326 RYRERGRDRDDRGDRDRSRGYGGSRGSNGGECFKCGKPGHFARECPSEG 472
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 37.7 bits (86), Expect = 0.003
Identities = 19/51 (37%), Positives = 26/51 (50%)
Frame = +1
Query: 203 TRGRNEHRGKGKGKSRSKSRSKGFDKSKYKCFLCHKQGHFKKDCPDKGGDG 253
+RG G G+G RS G D KC+ C + GHF ++C +GG G
Sbjct: 298 SRGGGGGGGGGRGGGRSGGGGGGSDM---KCYECGEPGHFARECRMRGGSG 441
>AV420911
Length = 418
Score = 35.4 bits (80), Expect = 0.013
Identities = 17/49 (34%), Positives = 24/49 (48%)
Frame = +2
Query: 198 EGLNVTRGRNEHRGKGKGKSRSKSRSKGFDKSKYKCFLCHKQGHFKKDC 246
+G N R H KG G R R +G + KC+ C + GHF ++C
Sbjct: 275 DGKNGWRVELSHNSKGGGGGRGGGRGRGGED--LKCYECGEPGHFAREC 415
>BP065763
Length = 537
Score = 34.3 bits (77), Expect = 0.028
Identities = 20/69 (28%), Positives = 31/69 (43%), Gaps = 4/69 (5%)
Frame = -1
Query: 185 LTKFKDMKVD----EGSEGLNVTRGRNEHRGKGKGKSRSKSRSKGFDKSKYKCFLCHKQG 240
L K KD + +G+ GR E + K ++ +G ++ C+ C K G
Sbjct: 522 LKKEKDHSIHLTTHDGASXSKAKPGRKEQK-----KDQALKVKEGRIHKEHVCYFCKKAG 358
Query: 241 HFKKDCPDK 249
HFKK CP +
Sbjct: 357 HFKKYCPKR 331
>AV773507
Length = 496
Score = 33.9 bits (76), Expect = 0.037
Identities = 19/64 (29%), Positives = 25/64 (38%)
Frame = +2
Query: 209 HRGKGKGKSRSKSRSKGFDKSKYKCFLCHKQGHFKKDCPDKGGDGSPSVQVAEASNEEGY 268
+RG G S G + CF C + GH +DCP GG G S+ Y
Sbjct: 278 YRGGGYSSGGRGSYGAGDRVGQDDCFECGRPGHRARDCPLAGGGGGRGRGGGSLSSRPRY 457
Query: 269 ESAG 272
+G
Sbjct: 458 GGSG 469
>TC18927 similar to PIR|AI2934|AI2934 chromate transport protein chrA
[imported] - Agrobacterium tumefaciens
(strain C58, Dupont) {Agrobacterium tumefaciens;},
partial (6%)
Length = 561
Score = 33.5 bits (75), Expect = 0.049
Identities = 18/47 (38%), Positives = 22/47 (46%), Gaps = 4/47 (8%)
Frame = -2
Query: 206 RNEHRGKGKGKSRSKS----RSKGFDKSKYKCFLCHKQGHFKKDCPD 248
R ++RG G S S R D S+ C C K+GHF CPD
Sbjct: 422 RPQNRGTSSGYSHSFGNFVPRPTQSDTSEIVCHRCSKKGHFANRCPD 282
>TC9435 similar to UP|Q9AXJ7 (Q9AXJ7) Phosphatase-like protein Mtc923,
partial (17%)
Length = 486
Score = 27.3 bits (59), Expect(2) = 0.20
Identities = 14/36 (38%), Positives = 18/36 (49%), Gaps = 2/36 (5%)
Frame = -2
Query: 199 GLNVTRGRNEHRGKGKGKSR--SKSRSKGFDKSKYK 232
G+ RGR GKGK R K RS+G + + K
Sbjct: 341 GIGARRGRERGSGKGKASVRETGKGRSQGIEARRVK 234
Score = 22.7 bits (47), Expect(2) = 0.20
Identities = 8/20 (40%), Positives = 12/20 (60%)
Frame = -3
Query: 248 DKGGDGSPSVQVAEASNEEG 267
++GGDG V++ EEG
Sbjct: 145 EEGGDGGVEVEIVAFEEEEG 86
>TC10378 similar to UP|Q84TG2 (Q84TG2) At3g16350, partial (26%)
Length = 442
Score = 31.2 bits (69), Expect = 0.24
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +2
Query: 219 SKSRSKGFDKSKYKCFLCHKQGHFKKDCPDKGGDGS 254
++SR G + +C C K+ H + CP +GG GS
Sbjct: 56 TESRGGGIGMGR-RCSHCSKENHNSRTCPSRGGGGS 160
>AV421607
Length = 245
Score = 30.8 bits (68), Expect = 0.31
Identities = 12/46 (26%), Positives = 20/46 (43%)
Frame = +3
Query: 211 GKGKGKSRSKSRSKGFDKSKYKCFLCHKQGHFKKDCPDKGGDGSPS 256
G G G + G C+ C + GH+ +DCP + +P+
Sbjct: 9 GSGPGSGSGSGTATG-------CYKCGRPGHWSRDCPSSAPNSNPN 125
>TC12416 similar to GB|AAH56066.1|33417132|BC056066 sop-prov protein
{Xenopus laevis;} , partial (32%)
Length = 416
Score = 30.4 bits (67), Expect = 0.41
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = +3
Query: 204 RGRNEHRGKGKGKSRSKSRSKG 225
RGR E RG+G+G+ R + R +G
Sbjct: 144 RGRGEGRGRGEGRGRGRGRGRG 209
Score = 27.7 bits (60), Expect = 2.7
Identities = 12/32 (37%), Positives = 17/32 (52%)
Frame = +3
Query: 194 DEGSEGLNVTRGRNEHRGKGKGKSRSKSRSKG 225
D G RGR E RG+G+G+ R + +G
Sbjct: 132 DGFGRGRGEGRGRGEGRGRGRGRGRGRRGRRG 227
>TC12851 UP|Q84TG9 (Q84TG9) Beta-tubulin, partial (34%)
Length = 591
Score = 29.3 bits (64), Expect = 0.92
Identities = 18/68 (26%), Positives = 30/68 (43%)
Frame = +2
Query: 198 EGLNVTRGRNEHRGKGKGKSRSKSRSKGFDKSKYKCFLCHKQGHFKKDCPDKGGDGSPSV 257
+G+ + RG N G G R G+ +++++ FLCH Q +GG+G V
Sbjct: 146 QGIGLRRGSNG*DNVG-GIYRQLDSHSGYLQARWQPFLCHVQAQGFPSLVHRGGNGRDGV 322
Query: 258 QVAEASNE 265
+E
Sbjct: 323 HRGGVQHE 346
>BP049555
Length = 555
Score = 28.9 bits (63), Expect = 1.2
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = +1
Query: 203 TRGRNEHRGKGKGKSRSKSRSK 224
T G ++ RGK +GKSR KSR K
Sbjct: 250 TSG*SQARGKDRGKSRGKSRGK 315
>BI418821
Length = 614
Score = 28.9 bits (63), Expect = 1.2
Identities = 21/81 (25%), Positives = 30/81 (36%)
Frame = +2
Query: 173 LEEVQAALRTKELTKFKDMKVDEGSEGLNVTRGRNEHRGKGKGKSRSKSRSKGFDKSKYK 232
L E A + TK D+ G+ L TR + RG G + + G
Sbjct: 215 LVEFSIATGDNDKTKAVDVTGPNGA-ALQPTRKDSAPRGFGGWRGGERRNGGGGGGGGGG 391
Query: 233 CFLCHKQGHFKKDCPDKGGDG 253
C+ C GH +DC +G
Sbjct: 392 CYNCGDTGHLARDCHRSNNNG 454
>TC12773
Length = 420
Score = 28.1 bits (61), Expect = 2.0
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = +2
Query: 217 SRSKSRSKGFDKSKYKCFLCHKQGHFKKDC 246
+R + +K S Y+C+ C + GH +DC
Sbjct: 299 NRHRWSNKRSSLSTYECWKCQRPGHMAEDC 388
>TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (62%)
Length = 684
Score = 27.7 bits (60), Expect = 2.7
Identities = 14/53 (26%), Positives = 23/53 (42%), Gaps = 10/53 (18%)
Frame = +2
Query: 204 RGRNEHRGKGKGKSRSKSRSKGFDKS----------KYKCFLCHKQGHFKKDC 246
RG E+ G+G + + G D K KC+ C +GH +++C
Sbjct: 320 RGNREYLGRGPPPGSGRCFNCGLDGHWARDCKAGDWKNKCYRCGDRGHVERNC 478
Score = 26.9 bits (58), Expect = 4.5
Identities = 13/41 (31%), Positives = 19/41 (45%)
Frame = +2
Query: 212 KGKGKSRSKSRSKGFDKSKYKCFLCHKQGHFKKDCPDKGGD 252
KG + + +G +CF C GH+ +DC K GD
Sbjct: 308 KGGPRGNREYLGRGPPPGSGRCFNCGLDGHWARDC--KAGD 424
>TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (56%)
Length = 598
Score = 27.7 bits (60), Expect = 2.7
Identities = 18/52 (34%), Positives = 24/52 (45%), Gaps = 1/52 (1%)
Frame = +1
Query: 202 VTRGRNEHRGKGKGKSRSKS-RSKGFDKSKYKCFLCHKQGHFKKDCPDKGGD 252
V RG E G G+ R + +G +CF C GH+ +DC K GD
Sbjct: 304 VPRGSREGGG---GRDRDREYMGRGPPPGSGRCFNCGIDGHWARDC--KAGD 444
Score = 26.6 bits (57), Expect = 5.9
Identities = 8/17 (47%), Positives = 13/17 (76%)
Frame = +1
Query: 230 KYKCFLCHKQGHFKKDC 246
K KC+ C ++GH +K+C
Sbjct: 448 KNKCYRCGERGHIEKNC 498
>BU494286
Length = 198
Score = 26.9 bits (58), Expect = 4.5
Identities = 13/39 (33%), Positives = 23/39 (58%)
Frame = +3
Query: 206 RNEHRGKGKGKSRSKSRSKGFDKSKYKCFLCHKQGHFKK 244
R+ R +G+ +SRS+SRS+ D+ + H G ++K
Sbjct: 60 RDRDRDRGRRRSRSRSRSRSRDRKE------HDGGDYRK 158
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.315 0.132 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,008,301
Number of Sequences: 28460
Number of extensions: 59781
Number of successful extensions: 445
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 403
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 430
length of query: 317
length of database: 4,897,600
effective HSP length: 90
effective length of query: 227
effective length of database: 2,336,200
effective search space: 530317400
effective search space used: 530317400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 55 (25.8 bits)
Medicago: description of AC135464.1