BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135461.9 + phase: 0
(375 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14274 weakly similar to GB|AAF79559.1|8778551|AC022464 F22G5.3... 64 3e-11
TC9920 similar to UP|Q9FF85 (Q9FF85) Emb|CAB80929.1, partial (34%) 58 2e-09
TC12360 similar to UP|O22196 (O22196) At2g40820 protein, partial... 52 1e-07
TC14454 similar to UP|O04133 (O04133) SRC2, partial (88%) 50 8e-07
TC20154 34 0.045
TC16662 weakly similar to UP|O80843 (O80843) At2g45760 protein, ... 33 0.10
TC15953 similar to UP|B2_DAUCA (P37707) B2 protein, partial (71%) 33 0.10
TC15052 32 0.22
BI418773 31 0.29
TC12971 similar to GB|AAN73294.1|25141199|BT002297 At5g12010/F14... 30 0.65
BG662475 25 1.4
BP047231 28 2.5
TC15735 similar to UP|Q9SI52 (Q9SI52) Expressed protein (At2g038... 28 3.2
CB827472 27 4.2
AV772298 27 4.2
AV780095 27 5.5
TC13011 similar to UP|Q9JM93 (Q9JM93) SRp25 nuclear protein, par... 27 5.5
TC18897 weakly similar to UP|Q9XEV3 (Q9XEV3) TIA-1 related prote... 27 5.5
AV419586 27 7.2
BI419494 26 9.4
>TC14274 weakly similar to GB|AAF79559.1|8778551|AC022464 F22G5.35
{Arabidopsis thaliana;} , partial (16%)
Length = 1208
Score = 64.3 bits (155), Expect = 3e-11
Identities = 49/170 (28%), Positives = 85/170 (49%), Gaps = 5/170 (2%)
Frame = +2
Query: 11 LEINVISAQDLAPVS---KSIKAYAVAWLNPERKLTTQADPHGHNNPTWNEKFVFRVDDD 67
L++ ++SA+ L V+ +K Y V W++P+R+L T++D G+ +P WNE+F +
Sbjct: 77 LDLTIVSAKHLKNVNWKTGDLKPYVVFWVDPDRRLATKSDDSGNTSPVWNERFTLPLSHP 256
Query: 68 FLLSEESVIMIEIYAS--AWLRDVLIGTVAVHLNNLLPRNRKSKIRFVALQVRRPSGRPQ 125
+S + +EI+ S + L+ TV + L +L + S IR L RPSGRP
Sbjct: 257 I---TDSFLTLEIFHSKPSDTPKPLVATVRLLLADLHDLHDSSSIRKFTL--LRPSGRPH 421
Query: 126 GILNIGVNVVDATMRSMPMYSELSSSAVEYYDITKPNKQNQNYDNNSNCD 175
G +++ + ++ R P+ D P+ N N + N NC+
Sbjct: 422 GKIHLKLGLLG---RPQPL------------DYPNPS-SNPNPNPNPNCN 523
>TC9920 similar to UP|Q9FF85 (Q9FF85) Emb|CAB80929.1, partial (34%)
Length = 825
Score = 58.2 bits (139), Expect = 2e-09
Identities = 40/138 (28%), Positives = 70/138 (49%), Gaps = 5/138 (3%)
Frame = +3
Query: 11 LEINVISAQDLAPVS---KSIKAYAVAWLNPERKLTTQADPHGHNNPTWNEKFVFRVDDD 67
LE + SA+ L V+ K YAV W++P+ K +T+AD +G W++ + +
Sbjct: 261 LETKLSSARGLKNVNWRHGPNKPYAVVWVDPKNKFSTRADENGDTEANWDQTLTIPLPPE 440
Query: 68 FLLSEESVIMIEI--YASAWLRDVLIGTVAVHLNNLLPRNRKSKIRFVALQVRRPSGRPQ 125
L E+ + I++ + S LIG+ + L +L AL ++RPSGRPQ
Sbjct: 441 PL--EDLTLFIDVVHFGSEEDTKPLIGSAKLKLREILDEAGIGDRASRALTLKRPSGRPQ 614
Query: 126 GILNIGVNVVDATMRSMP 143
G +++ V + ++ R+ P
Sbjct: 615 GKVDVKVAIKESGYRAPP 668
>TC12360 similar to UP|O22196 (O22196) At2g40820 protein, partial (11%)
Length = 1030
Score = 52.4 bits (124), Expect = 1e-07
Identities = 35/137 (25%), Positives = 67/137 (48%), Gaps = 11/137 (8%)
Frame = +3
Query: 11 LEINVISAQDLA--PVSKSIKAYAVAWLNPERKLTTQADPHGHNNPTWNEKFVFRVDDDF 68
+EI +ISA+ + P + YAV W++P K T+ D G+ NP W KF +VD +
Sbjct: 69 VEICLISARGVKGPPSLWKRQWYAVGWVDPSTKYCTKFDASGNANPVWRTKFAVQVDSEV 248
Query: 69 LLSEESVIMIEIYA--SAWLRDVLIGTVAVHLNNLLPRN-------RKSKIRFVALQVRR 119
++ + +E+Y+ + + L G+ V L L + R ++ L+ ++
Sbjct: 249 PNFKDLSLNVEVYSRDPVFFTEKLHGSATVLLKEFLAKQLQSSGIPRNEEVGSYQLR-KK 425
Query: 120 PSGRPQGILNIGVNVVD 136
S +P G +++ + V +
Sbjct: 426 KSNKPSGFVDVSIRVFE 476
>TC14454 similar to UP|O04133 (O04133) SRC2, partial (88%)
Length = 1190
Score = 49.7 bits (117), Expect = 8e-07
Identities = 38/133 (28%), Positives = 63/133 (46%), Gaps = 11/133 (8%)
Frame = +1
Query: 8 FQLLEINVISAQDLAPVS--KSIKAYAVAWLNPERKLTTQADPHGH----NNPTWNEKFV 61
++ LE+N+ISA+DL V+ + YAV ++ + A H H +PTWN
Sbjct: 187 YRKLELNIISAKDLKDVNLFSKMDVYAVVSVSGDPLHPQTATTHVHKDSGTSPTWNFPVK 366
Query: 62 FRVDDDFLLSEESVIMIEIYASAWLR-DVLIGTVAVHLNNLLPR----NRKSKIRFVALQ 116
F V++ + +++ + L D +IGTV + + LL R V+ Q
Sbjct: 367 FVVNETLAQQNRLSLEVKLLSDRTLAGDTVIGTVHIPIKELLDNPGGGGGGDSFRQVSYQ 546
Query: 117 VRRPSGRPQGILN 129
V+R SG+ +G N
Sbjct: 547 VKRSSGKAKGTFN 585
>TC20154
Length = 607
Score = 33.9 bits (76), Expect = 0.045
Identities = 18/68 (26%), Positives = 32/68 (46%)
Frame = +3
Query: 11 LEINVISAQDLAPVSKSIKAYAVAWLNPERKLTTQADPHGHNNPTWNEKFVFRVDDDFLL 70
L + V+ A DL P A ++ + + H NP WNEKFVF +++ L
Sbjct: 186 LVVEVLDASDLMPKDGEGSASPFVEVDFDEQQQRTQTKHKDLNPYWNEKFVFNINNPRDL 365
Query: 71 SEESVIMI 78
+++ ++
Sbjct: 366 PHQTIEVV 389
>TC16662 weakly similar to UP|O80843 (O80843) At2g45760 protein, partial
(13%)
Length = 816
Score = 32.7 bits (73), Expect = 0.10
Identities = 32/139 (23%), Positives = 62/139 (44%), Gaps = 15/139 (10%)
Frame = +3
Query: 11 LEINVISAQDL-------------APVSKSIKAYAVAWLNPERKLTTQADPHGHNNPTWN 57
LE+ V+SA+DL ++SI +YA T ++ + HNN +WN
Sbjct: 111 LELTVLSAEDLRVDRNLNTENIYVVVRAESIHSYATG--------TAGSNSNSHNNFSWN 266
Query: 58 EKFVFRVDDDFLLSEESVIM-IEIYASAWLRDVLIGTVAVHLNNLLPRNRKSK-IRFVAL 115
EK + D + S+ + ++ S IG V +++ L + ++F++
Sbjct: 267 EKLLV----DMPVHARSITLEVKCKTSRGSASKDIGVARVAVSDFLGGSVPDDCLQFLSY 434
Query: 116 QVRRPSGRPQGILNIGVNV 134
++R G+ G++N V +
Sbjct: 435 RLRNWEGKRNGVVNFSVRL 491
>TC15953 similar to UP|B2_DAUCA (P37707) B2 protein, partial (71%)
Length = 919
Score = 32.7 bits (73), Expect = 0.10
Identities = 15/38 (39%), Positives = 20/38 (52%)
Frame = +1
Query: 162 NKQNQNYDNNSNCDAKHMMTLQRSQSEKNDSTINDYVY 199
N N N NN + DAK TL S+S + TI Y++
Sbjct: 109 NNNNNNDSNNKSKDAKKFKTLPASESLPRNETIGGYIF 222
>TC15052
Length = 1846
Score = 31.6 bits (70), Expect = 0.22
Identities = 32/130 (24%), Positives = 54/130 (40%), Gaps = 8/130 (6%)
Frame = +3
Query: 33 VAWL-NPERKLTTQADPHGHNNPTWNEKFVFRVDDDFLLSEESVIMIEIY----ASAWLR 87
+ W NPE ++T+ G NP +NE V + ES + EI+ +L
Sbjct: 540 IGWTSNPENTVSTKTINGGGRNPVFNENLRLSVK-----TVESSLKCEIWMLSRVKNYLE 704
Query: 88 DVLIGTVAVHLNNLLPRNRKSKIRFVALQVRRPSGRPQGILNIGVNVVDAT---MRSMPM 144
D L+G V L+ +L + + + +L P G + + + A+ M M
Sbjct: 705 DQLLGFALVPLSEVLLKKDEKLEKEFSLSSTDLFHTPAGFVQLSLAYAGASPDVMAISAM 884
Query: 145 YSELSSSAVE 154
SEL ++ E
Sbjct: 885 PSELDTNCTE 914
>BI418773
Length = 561
Score = 31.2 bits (69), Expect = 0.29
Identities = 22/63 (34%), Positives = 32/63 (49%), Gaps = 3/63 (4%)
Frame = +3
Query: 7 PFQLLEINVISAQDL--APVSKSIKAYAV-AWLNPERKLTTQADPHGHNNPTWNEKFVFR 63
P LE+ ++SA+ L + +I Y + A+ E K T Q +NP WNE F+F
Sbjct: 189 PRGTLEVVLVSAKGLDDSEFLSNIDPYVILAYKAQEHKSTVQEG--AGSNPQWNETFLFT 362
Query: 64 VDD 66
V D
Sbjct: 363 VSD 371
>TC12971 similar to GB|AAN73294.1|25141199|BT002297 At5g12010/F14F18_180
{Arabidopsis thaliana;}, partial (7%)
Length = 721
Score = 30.0 bits (66), Expect = 0.65
Identities = 20/71 (28%), Positives = 32/71 (44%)
Frame = +3
Query: 144 MYSELSSSAVEYYDITKPNKQNQNYDNNSNCDAKHMMTLQRSQSEKNDSTINDYVYNPNG 203
+Y+ L SS + D PN N N NN+N A + T +R + K D + N
Sbjct: 117 VYTLLQSSFDQMND---PNNNNNNNYNNNNSSATSVNTRKRRRKVKGDDDVGGDEIPSNN 287
Query: 204 KNGYGGECESE 214
+ G+ +S+
Sbjct: 288 REEEEGDNKSK 320
>BG662475
Length = 490
Score = 25.4 bits (54), Expect(2) = 1.4
Identities = 24/97 (24%), Positives = 39/97 (39%)
Frame = +3
Query: 165 NQNYDNNSNCDAKHMMTLQRSQSEKNDSTINDYVYNPNGKNGYGGECESEISVPTGKKGV 224
+ N S+ +H + Q E DY + KNG S++S+ G+
Sbjct: 153 HSNSPKISHLPPQHHTKVNTQQGEGEIPIKFDYSTPESKKNG-----NSKVSMTQGQASK 317
Query: 225 IVNANGSLCSDVGPSPSVVAAAIAKGLYPLPLHVPRK 261
+ + VG S + +A + PLP HVPR+
Sbjct: 318 VASPQ------VGTSET--RGKVAAKVEPLPQHVPRQ 404
Score = 21.9 bits (45), Expect(2) = 1.4
Identities = 9/36 (25%), Positives = 19/36 (52%)
Frame = +1
Query: 138 TMRSMPMYSELSSSAVEYYDITKPNKQNQNYDNNSN 173
T + +Y+E S+ ++ ++KP+ + N N N
Sbjct: 25 TTPQVSLYAEKSAKHLQAIQVSKPSIASNNTTENLN 132
>BP047231
Length = 476
Score = 28.1 bits (61), Expect = 2.5
Identities = 10/22 (45%), Positives = 13/22 (58%)
Frame = +2
Query: 41 KLTTQADPHGHNNPTWNEKFVF 62
KL HN PTWN+K++F
Sbjct: 92 KLYQDEKKQEHNQPTWNQKWIF 157
>TC15735 similar to UP|Q9SI52 (Q9SI52) Expressed protein (At2g03890/T18C20.9),
partial (22%)
Length = 1187
Score = 27.7 bits (60), Expect = 3.2
Identities = 17/39 (43%), Positives = 20/39 (50%)
Frame = -2
Query: 133 NVVDATMRSMPMYSELSSSAVEYYDITKPNKQNQNYDNN 171
N + A +R +YSE SS T PN NQNY NN
Sbjct: 1036 NRLTA*LRPSNIYSESFSSYHRSQLSTHPNLINQNYPNN 920
>CB827472
Length = 544
Score = 27.3 bits (59), Expect = 4.2
Identities = 25/102 (24%), Positives = 47/102 (45%)
Frame = +1
Query: 1 MSMLAPPFQLLEINVISAQDLAPVSKSIKAYAVAWLNPERKLTTQADPHGHNNPTWNEKF 60
MS+ P ++ ++ +D +S+ V + N + + T D G NP + EKF
Sbjct: 22 MSIQGQPLEVTVVSCSKLKDTEWISRQDPYVCVEYGNTKFRTRTCTD--GGRNPVFQEKF 195
Query: 61 VFRVDDDFLLSEESVIMIEIYASAWLRDVLIGTVAVHLNNLL 102
F + + L + +VI+ ++ D IGT + L+ +L
Sbjct: 196 TFPLIEG--LRDINVIVWNSNTLSF--DDFIGTGKIQLHKVL 309
>AV772298
Length = 528
Score = 27.3 bits (59), Expect = 4.2
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +2
Query: 324 NRRQGSGLFSCFGTALGCEI 343
NR G G FSC G AL C+I
Sbjct: 425 NRVSGKGRFSCGGGALQCDI 484
>AV780095
Length = 511
Score = 26.9 bits (58), Expect = 5.5
Identities = 15/44 (34%), Positives = 21/44 (47%)
Frame = -1
Query: 38 PERKLTTQADPHGHNNPTWNEKFVFRVDDDFLLSEESVIMIEIY 81
P +K T Q + NP WNEKF V D V+ +++Y
Sbjct: 331 PAKKTTVQRK---NLNPVWNEKFKLIVKD----PHSQVLQLQVY 221
>TC13011 similar to UP|Q9JM93 (Q9JM93) SRp25 nuclear protein, partial (10%)
Length = 593
Score = 26.9 bits (58), Expect = 5.5
Identities = 18/65 (27%), Positives = 26/65 (39%), Gaps = 3/65 (4%)
Frame = -3
Query: 299 DHLGNNNNGSV---KKTGKQTKGKGKGKNRRQGSGLFSCFGTALGCEISITCGGGNHKKS 355
DH + NG + G +T GKG+GK ++ G G + G+ K
Sbjct: 471 DHSEDEVNGEAEVKRSRGGKTSGKGRGKAPKKSGAEVKGSGRGRGGAAAAANKSGSSGKR 292
Query: 356 GSTNK 360
GS K
Sbjct: 291 GSPRK 277
>TC18897 weakly similar to UP|Q9XEV3 (Q9XEV3) TIA-1 related protein, partial
(5%)
Length = 481
Score = 26.9 bits (58), Expect = 5.5
Identities = 14/36 (38%), Positives = 18/36 (49%), Gaps = 4/36 (11%)
Frame = -1
Query: 295 PQVYDHLG----NNNNGSVKKTGKQTKGKGKGKNRR 326
P ++LG G+V K T GKGKGK+ R
Sbjct: 184 PSTAENLG*IL*KKEGGNVTSAHKNTNGKGKGKHDR 77
>AV419586
Length = 329
Score = 26.6 bits (57), Expect = 7.2
Identities = 11/33 (33%), Positives = 19/33 (57%)
Frame = +1
Query: 25 SKSIKAYAVAWLNPERKLTTQADPHGHNNPTWN 57
S + + +V+ LNP++K T + P +P WN
Sbjct: 187 STHVLSASVSALNPQKKETAHSVPVFQTSPNWN 285
>BI419494
Length = 478
Score = 26.2 bits (56), Expect = 9.4
Identities = 18/63 (28%), Positives = 28/63 (43%)
Frame = -1
Query: 232 LCSDVGPSPSVVAAAIAKGLYPLPLHVPRKTVNNSMFEKWPPEKDNGGEMLNTKMDRWRQ 291
LCS + PS + PLPL + +S+ + PP +LN K R+++
Sbjct: 448 LCSSILPSK----------IKPLPLFFITCPLQHSLHSRVPPHLSFLNPLLNRKTHRFQR 299
Query: 292 IDI 294
I I
Sbjct: 298 ITI 290
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.313 0.132 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,700,166
Number of Sequences: 28460
Number of extensions: 97347
Number of successful extensions: 417
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 412
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 414
length of query: 375
length of database: 4,897,600
effective HSP length: 92
effective length of query: 283
effective length of database: 2,279,280
effective search space: 645036240
effective search space used: 645036240
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 56 (26.2 bits)
Medicago: description of AC135461.9