BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135461.4 + phase: 0
(631 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV411443 182 2e-46
TC11192 similar to GB|AAO63323.1|28950799|BT005259 At2g03430 {Ar... 33 0.14
TC17487 UP|CAE45597 (CAE45597) SAR DNA-binding protein-like prot... 31 0.53
BI417326 31 0.69
TC16203 UP|Q8GRU6 (Q8GRU6) LRR receptor-like kinase (Hypernodula... 31 0.69
TC11401 30 0.90
CB829198 30 1.2
TC7807 homologue to UP|Q946J9 (Q946J9) Aquaporin protein PIP1,1,... 30 1.5
BP085186 29 2.0
TC8185 similar to UP|Q9FLV3 (Q9FLV3) Similarity to ribosomal pro... 29 2.0
BP054863 29 2.6
TC14871 similar to UP|Q85HQ0 (Q85HQ0) NADH dehydrogenase subunit... 29 2.6
TC8167 similar to PIR|JC7519|JC7519 subtilisin-like serine prote... 28 3.4
TC11310 similar to UP|BAD11331 (BAD11331) BRI1-KD interacting pr... 28 3.4
TC10380 similar to UP|Q8S8U2 (Q8S8U2) Ycf1 protein, partial (40%) 28 4.5
TC16662 weakly similar to UP|O80843 (O80843) At2g45760 protein, ... 28 4.5
AV409241 28 5.8
TC17433 27 7.6
TC12210 similar to UP|Q867T7 (Q867T7) P67-like superoxide-genera... 27 7.6
BP035958 27 9.9
>AV411443
Length = 429
Score = 182 bits (461), Expect = 2e-46
Identities = 96/122 (78%), Positives = 107/122 (87%)
Frame = +1
Query: 392 KNPNQRSSDQQKLQPEFPNDDHGEMKSKKGKDQNLKKKKKKGASNESKSESEFKKGVRPV 451
++ NQRS DQQKL EF +D G+ SKKGKD+N KKKKKGA +E K+ESE+KKG+RPV
Sbjct: 73 ESSNQRSGDQQKLASEFLKEDSGD--SKKGKDKN-SKKKKKGAVSEPKNESEYKKGLRPV 243
Query: 452 LWLTQDFPLKTDELLPLLDILANKVKAIRRLRELLTTKLPPGTFPVKVAIPIVPTIRVIV 511
LWLT DFPL+T+ELLPLLDILANKVKAIRRLRELLTTKLP GTFPVKVAIPIVPTIRVIV
Sbjct: 244 LWLTHDFPLETNELLPLLDILANKVKAIRRLRELLTTKLPYGTFPVKVAIPIVPTIRVIV 423
Query: 512 TF 513
TF
Sbjct: 424 TF 429
>TC11192 similar to GB|AAO63323.1|28950799|BT005259 At2g03430 {Arabidopsis
thaliana;}, partial (70%)
Length = 597
Score = 33.1 bits (74), Expect = 0.14
Identities = 23/77 (29%), Positives = 38/77 (48%)
Frame = +3
Query: 44 SESLSAEIQADQISSVIDRRDVPGRETPLHLTVRLRDPIAAEILMSAGADWSLQNEQGWS 103
S+ + + AD +SV++ D G PLH + + E L+S GAD +L+N G +
Sbjct: 225 SQVVKILLSADASASVVNSADDEGW-APLHSAASIGNLEIVEALLSKGADVNLKNGGGRT 401
Query: 104 SLQEAVCNREESIALVI 120
+L A IA ++
Sbjct: 402 ALHYAASKGRVKIAEIL 452
>TC17487 UP|CAE45597 (CAE45597) SAR DNA-binding protein-like protein, complete
Length = 1794
Score = 31.2 bits (69), Expect = 0.53
Identities = 33/122 (27%), Positives = 52/122 (42%), Gaps = 5/122 (4%)
Frame = +1
Query: 323 ANGEDYDDVLTAEERVQLDSALRMGNSDGVCQDEEPGAGGGGFDGRGNL--YENCE---V 377
A G+ D+ + E R +L+ LR+ N +G +E G G G+ + Y+
Sbjct: 1252 ALGDGVDNTMGLENRAKLE--LRLRNLEG----KELGRFAGSAKGKPKIEAYDKDRKKGA 1413
Query: 378 NGVVKEKKGWFGWNKNPNQRSSDQQKLQPEFPNDDHGEMKSKKGKDQNLKKKKKKGASNE 437
G++ K + NP+ + QK D H KK + + KKKK+K NE
Sbjct: 1414 GGLITPAKTY-----NPSADAVISQKSDSAMDEDTHEPSADKKKEKKEKKKKKEK---NE 1569
Query: 438 SK 439
K
Sbjct: 1570 EK 1575
>BI417326
Length = 478
Score = 30.8 bits (68), Expect = 0.69
Identities = 27/113 (23%), Positives = 50/113 (43%), Gaps = 3/113 (2%)
Frame = +2
Query: 14 VAVLNRDHASLRRLIST---IPTLPKPGEVTTESESLSAEIQADQISSVIDRRDVPGRET 70
VA D AS+ + + L G++ +E L A S ++ RD+ GR T
Sbjct: 122 VAAAAADDASVTEAVDPAVRLMYLANEGDLEGITELLDA-------GSDVNFRDIDGR-T 277
Query: 71 PLHLTVRLRDPIAAEILMSAGADWSLQNEQGWSSLQEAVCNREESIALVIARY 123
LH+ R ++L+ GAD Q+ G + L +A+ + + ++ ++
Sbjct: 278 ALHVAACHRRTDVVDLLLQRGADVDTQDRWGSTPLADALYYKNHDVVKLLEKH 436
>TC16203 UP|Q8GRU6 (Q8GRU6) LRR receptor-like kinase (Hypernodulation aberrant
root formation protein), complete
Length = 3308
Score = 30.8 bits (68), Expect = 0.69
Identities = 16/47 (34%), Positives = 23/47 (48%), Gaps = 2/47 (4%)
Frame = +1
Query: 393 NPNQRSSDQQKLQPEF--PNDDHGEMKSKKGKDQNLKKKKKKGASNE 437
NPN S Q+ QP PNDDH S G+ Q + +++ +E
Sbjct: 1624 NPNAHQSQHQRQQPHRSDPNDDHSPCFSDGGRPQPEQPRRRGSEGDE 1764
>TC11401
Length = 488
Score = 30.4 bits (67), Expect = 0.90
Identities = 14/27 (51%), Positives = 18/27 (65%)
Frame = +1
Query: 583 PSDYKWVDANERKRRMKAKRAKIKKNK 609
PSDY WV A +K++M+ K K KK K
Sbjct: 1 PSDYTWVTAEAKKKKMQEKN-KSKKGK 78
>CB829198
Length = 562
Score = 30.0 bits (66), Expect = 1.2
Identities = 19/58 (32%), Positives = 28/58 (47%), Gaps = 1/58 (1%)
Frame = +3
Query: 391 NKNPNQRSSDQQKLQPEFPNDDHGEMKSKKGKDQNL-KKKKKKGASNESKSESEFKKG 447
N + + K + E D+ G+ ++ K K KKKK K SN+S +E KKG
Sbjct: 267 NDDVEESQHSDSKEETEEEEDEDGDNENNKPKRAKAAKKKKNKERSNKSDNEVVKKKG 440
>TC7807 homologue to UP|Q946J9 (Q946J9) Aquaporin protein PIP1,1, partial
(27%)
Length = 1289
Score = 29.6 bits (65), Expect = 1.5
Identities = 17/52 (32%), Positives = 25/52 (47%)
Frame = +2
Query: 528 PLSSPVSFQDAKYKESEGSTSWVSWMKGSRGMPSSDSDSHIHSHRYKDEVDP 579
PLS PV+F +K+ +W S + M S + HI + KD+ DP
Sbjct: 350 PLSFPVTFSPSKHLRRNHLPAWSSTRSPIKSM--STTVEHIVLFKVKDDTDP 499
>BP085186
Length = 388
Score = 29.3 bits (64), Expect = 2.0
Identities = 13/32 (40%), Positives = 19/32 (58%)
Frame = -2
Query: 415 EMKSKKGKDQNLKKKKKKGASNESKSESEFKK 446
EMK KK K + KKKKKK +++ + K+
Sbjct: 264 EMKKKKKKKKKKKKKKKKKKKKQNQRKKRMKR 169
>TC8185 similar to UP|Q9FLV3 (Q9FLV3) Similarity to ribosomal protein 30S
subunit, partial (55%)
Length = 1268
Score = 29.3 bits (64), Expect = 2.0
Identities = 21/80 (26%), Positives = 35/80 (43%), Gaps = 8/80 (10%)
Frame = +1
Query: 38 GEVTTESESLSAEIQADQISSVIDRR--------DVPGRETPLHLTVRLRDPIAAEILMS 89
G V E +S + D +SS+I R+ GR ++R+P+ E+ +
Sbjct: 508 GVVRAEEDSETTYASIDLVSSIIQRKLRKIKEKVSDHGRHMKGFNRAKVREPVEVEVPLE 687
Query: 90 AGADWSLQNEQGWSSLQEAV 109
D S E+G ++L E V
Sbjct: 688 EDDDISPLEEEGEATLVEVV 747
>BP054863
Length = 498
Score = 28.9 bits (63), Expect = 2.6
Identities = 21/60 (35%), Positives = 29/60 (48%)
Frame = -3
Query: 427 KKKKKKGASNESKSESEFKKGVRPVLWLTQDFPLKTDELLPLLDILANKVKAIRRLRELL 486
K+KKK + K ESE K PV WL D ++ D LL ++ K K + + E L
Sbjct: 475 KRKKKTKLKKKRKQESEVVKSSIPV-WLIGDL-MELDATKDLLPLIKGKRKREKMVVEFL 302
>TC14871 similar to UP|Q85HQ0 (Q85HQ0) NADH dehydrogenase subunit 4, partial
(4%)
Length = 588
Score = 28.9 bits (63), Expect = 2.6
Identities = 25/71 (35%), Positives = 30/71 (42%), Gaps = 1/71 (1%)
Frame = -2
Query: 403 KLQPEFPNDDHGEMKSKKGKDQNLKKKKKKGASNESKSESEFKKGVRPVLWLTQDF-PLK 461
KLQ EFP + K+ +N KKKK+K KG P L +D PL
Sbjct: 524 KLQKEFPQRGIYIKRQKRKNKKNGKKKKRKERKTTGTPTPNDLKG--PTLTRPKDSKPLL 351
Query: 462 TDELLPLLDIL 472
T LPL L
Sbjct: 350 TSISLPLFSPL 318
>TC8167 similar to PIR|JC7519|JC7519 subtilisin-like serine proteinase -
Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (65%)
Length = 2760
Score = 28.5 bits (62), Expect = 3.4
Identities = 17/58 (29%), Positives = 29/58 (49%), Gaps = 2/58 (3%)
Frame = +1
Query: 11 PAHVAVLNRDHASLRRLISTI--PTLPKPGEVTTESESLSAEIQADQISSVIDRRDVP 66
P AV + + RR+ +T+ PTLP P T +S+ A+ + + + RR +P
Sbjct: 673 PVTTAVSEKRESHRRRVTATVTAPTLPAPPRDPTSGTLVSSVTLAEPLEAWLHRRVLP 846
>TC11310 similar to UP|BAD11331 (BAD11331) BRI1-KD interacting protein 102
(Fragment), partial (13%)
Length = 667
Score = 28.5 bits (62), Expect = 3.4
Identities = 25/76 (32%), Positives = 37/76 (47%), Gaps = 4/76 (5%)
Frame = +1
Query: 376 EVNGVVKEKKGWFGWNK-NPNQRSSDQQKLQPEFPNDDHGEMKSKKGK---DQNLKKKKK 431
EV G KEKK ++ N SSD++K + E ++K + G DQ+ KKKKK
Sbjct: 67 EVEGEKKEKKKKKKKDQENGEVASSDEEKAEKE--KKKKHKVKVEDGSPDLDQSEKKKKK 240
Query: 432 KGASNESKSESEFKKG 447
K + + +E G
Sbjct: 241 KKDQDAEDNAAEISNG 288
>TC10380 similar to UP|Q8S8U2 (Q8S8U2) Ycf1 protein, partial (40%)
Length = 5664
Score = 28.1 bits (61), Expect = 4.5
Identities = 14/23 (60%), Positives = 17/23 (73%), Gaps = 1/23 (4%)
Frame = +2
Query: 411 DDHGEMKSKK-GKDQNLKKKKKK 432
+D+GE SKK GKD+N KK K K
Sbjct: 4562 EDYGESDSKKGGKDKNKKKYKNK 4630
>TC16662 weakly similar to UP|O80843 (O80843) At2g45760 protein, partial
(13%)
Length = 816
Score = 28.1 bits (61), Expect = 4.5
Identities = 18/57 (31%), Positives = 27/57 (46%)
Frame = -3
Query: 491 PPGTFPVKVAIPIVPTIRVIVTFTKFEELQTPEEFSTPLSSPVSFQDAKYKESEGST 547
PP T P+++ P PT V T+ L E+ +TPL P F + K + S+
Sbjct: 577 PPVTEPLEI*NPQEPTTISGVAATRSRVLSLTEKLTTPLRFPSQFLNR*LKNCKQSS 407
>AV409241
Length = 282
Score = 27.7 bits (60), Expect = 5.8
Identities = 21/64 (32%), Positives = 25/64 (38%), Gaps = 6/64 (9%)
Frame = -1
Query: 319 EEVFANGEDYDDVLTA----EERVQLDSALRMGNSDGVCQDEEP--GAGGGGFDGRGNLY 372
EEV G D +V+ E + + DGV E G GGGG G G L
Sbjct: 273 EEVVGGGADLGEVVDVGIFREGFAESGEGVAAAGGDGVGGGETVAGGGGGGGVGGGGVLV 94
Query: 373 ENCE 376
CE
Sbjct: 93 GECE 82
>TC17433
Length = 637
Score = 27.3 bits (59), Expect = 7.6
Identities = 26/84 (30%), Positives = 33/84 (38%), Gaps = 15/84 (17%)
Frame = +3
Query: 452 LWLTQDFPLKTDELLPLLDILANKVKA-------IRRLRELLTTK--------LPPGTFP 496
LW +QD PL D P +KV I+ L E LT+ P FP
Sbjct: 288 LWSSQDDPLTVDYCSPDKIDSMHKVTLEVKGEPPIKNLSEKLTSGGIIHQLWIEQPENFP 467
Query: 497 VKVAIPIVPTIRVIVTFTKFEELQ 520
+A PT+ I F + E LQ
Sbjct: 468 TCLATKPYPTVSCISVFQEVETLQ 539
>TC12210 similar to UP|Q867T7 (Q867T7) P67-like superoxide-generating NADPH
oxidase, partial (3%)
Length = 416
Score = 27.3 bits (59), Expect = 7.6
Identities = 17/43 (39%), Positives = 18/43 (41%)
Frame = -3
Query: 347 GNSDGVCQDEEPGAGGGGFDGRGNLYENCEVNGVVKEKKGWFG 389
G D V DEE G G DGR C V+ GWFG
Sbjct: 183 GGDDEVEDDEEDGGGEPAVDGRPECG*GC--YRFVEHGFGWFG 61
>BP035958
Length = 574
Score = 26.9 bits (58), Expect = 9.9
Identities = 20/50 (40%), Positives = 27/50 (54%), Gaps = 3/50 (6%)
Frame = -3
Query: 323 ANGEDYDDVL---TAEERVQLDSALRMGNSDGVCQDEEPGAGGGGFDGRG 369
A GE+ DV+ AE RV LD + + + ++EEP AGG G G G
Sbjct: 335 AEGEEVGDVV*IEDAEGRVLLDLNRDL-DERLLAEEEEP*AGGVGLGGGG 189
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.314 0.132 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,132,800
Number of Sequences: 28460
Number of extensions: 136255
Number of successful extensions: 801
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 727
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 762
length of query: 631
length of database: 4,897,600
effective HSP length: 96
effective length of query: 535
effective length of database: 2,165,440
effective search space: 1158510400
effective search space used: 1158510400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 58 (26.9 bits)
Medicago: description of AC135461.4