BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135320.16 + phase: 0
(153 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17737 similar to UP|FER3_MAIZE (P27788) Ferredoxin III, chloro... 86 2e-18
TC8076 similar to UP|FER2_ARATH (O04090) Ferredoxin 2, chloropla... 79 5e-16
TC8077 similar to UP|FER1_PEA (P09911) Ferredoxin I, chloroplast... 77 1e-15
AV776282 30 0.14
TC14242 homologue to UP|PSAF_SPIOL (P12355) Photosystem I reacti... 29 0.41
AV419985 28 0.70
TC7804 similar to UP|Q9M600 (Q9M600) Chlorophyll a/b binding pro... 27 1.2
AW719654 27 2.0
BF177505 27 2.0
BP055490 26 2.7
TC9100 similar to UP|Q8W544 (Q8W544) NADH-ubiquinone oxidoreduct... 26 2.7
TC9676 similar to PIR|S71223|S71223 xyloglucan endo-1,4-beta-D-g... 26 2.7
TC9418 similar to UP|Q94JQ3 (Q94JQ3) AT4g32520/F8B4_220 (Serine... 26 2.7
AV768401 25 4.5
BP048304 25 4.5
TC12300 weakly similar to UP|Q8BKL0 (Q8BKL0) Splicing factor, pa... 25 5.9
TC12700 similar to UP|P93390 (P93390) Phosphate/phosphoenolpyruv... 25 7.7
TC16150 similar to GB|AAO63328.1|28950809|BT005264 At4g25840 {Ar... 25 7.7
TC12781 weakly similar to UP|Q940Z5 (Q940Z5) AT5g39050/MXF12_60,... 25 7.7
>TC17737 similar to UP|FER3_MAIZE (P27788) Ferredoxin III, chloroplast
precursor (Fd III), partial (72%)
Length = 736
Score = 86.3 bits (212), Expect = 2e-18
Identities = 45/116 (38%), Positives = 70/116 (59%), Gaps = 2/116 (1%)
Frame = +2
Query: 36 GSHPSSPSLTFTVRSSYKV-VIEHEGKTTQLEVEPDETILSKALDSGLDVPHDCKLGVCM 94
G S+PS T ++YK+ ++ +GK + E E D IL A ++G+++P+ C+ G C
Sbjct: 191 GLKSSTPSRV-TAMAAYKIKLVGPDGKENEFEAEDDVYILDAAENAGVELPYSCRAGACS 367
Query: 95 TCPARLISGTVDQSDG-MLSDDVVERGYALLCASYPRSDCHIRVIPEDELLSMQLA 149
TC ++++G+VDQSDG L D+ ++ G+ L C SYP +DC I E EL LA
Sbjct: 368 TCAGKIVTGSVDQSDGSFLDDNQLKDGFVLTCVSYPTADCVIETHKEGELY*EMLA 535
>TC8076 similar to UP|FER2_ARATH (O04090) Ferredoxin 2, chloroplast
precursor, partial (78%)
Length = 717
Score = 78.6 bits (192), Expect = 5e-16
Identities = 46/138 (33%), Positives = 68/138 (48%), Gaps = 1/138 (0%)
Frame = +2
Query: 7 TPLTLFRQKHPTTKLPYPSQLNTRPRLGSGSHPSSPSLTFTVRSSYKVVIEHEGKTTQLE 66
T T F ++ P P Q+ + GS ++YKV + + E
Sbjct: 62 TVSTSFLRRQPVAAFPNVGQVMFGVKGGSRGGR-------VTMAAYKVKLITPEGPQEFE 220
Query: 67 VEPDETILSKALDSGLDVPHDCKLGVCMTCPARLISGTVDQSDG-MLSDDVVERGYALLC 125
D IL +A + G+D+P+ C+ G C +C +++ G VDQSDG L DD VE G+ L C
Sbjct: 221 CPDDVYILDQAEEVGIDIPYSCRAGSCSSCAGKVVEGAVDQSDGSFLDDDQVEGGFVLTC 400
Query: 126 ASYPRSDCHIRVIPEDEL 143
+YP+SD I E+EL
Sbjct: 401 VAYPKSDVVIETHKEEEL 454
>TC8077 similar to UP|FER1_PEA (P09911) Ferredoxin I, chloroplast
precursor, partial (81%)
Length = 703
Score = 77.0 bits (188), Expect = 1e-15
Identities = 43/135 (31%), Positives = 65/135 (47%), Gaps = 1/135 (0%)
Frame = +1
Query: 10 TLFRQKHPTTKLPYPSQLNTRPRLGSGSHPSSPSLTFTVRSSYKVVIEHEGKTTQLEVEP 69
T F ++ P P Q + G G ++YKV + + E
Sbjct: 115 TSFLRRQPLKAFPNVGQALFGLKSGCGGR--------VTMAAYKVKLITPEGPFEFECPD 270
Query: 70 DETILSKALDSGLDVPHDCKLGVCMTCPARLISGTVDQSDG-MLSDDVVERGYALLCASY 128
D IL A + G+D+P+ C+ G C +C +++ G VDQSDG L DD ++ G+ L C +Y
Sbjct: 271 DVYILDHAEEQGIDIPYSCRAGSCSSCAGKVVGGNVDQSDGSFLDDDQIDAGFVLTCVAY 450
Query: 129 PRSDCHIRVIPEDEL 143
P+SD I E+EL
Sbjct: 451 PQSDVVIETHKEEEL 495
>AV776282
Length = 564
Score = 30.4 bits (67), Expect = 0.14
Identities = 18/61 (29%), Positives = 31/61 (50%), Gaps = 3/61 (4%)
Frame = -2
Query: 43 SLTFTVRSSYKVVIEHEGKTTQLEVEPDETILSKA---LDSGLDVPHDCKLGVCMTCPAR 99
S+ + +Y+ + G+ + E E T+LS ++ LD+ H +LGVC T P R
Sbjct: 341 SIKNNLEPNYQYFVVEMGREMK-ETERISTVLSHLA*KIELSLDINHVLELGVCFTLPCR 165
Query: 100 L 100
+
Sbjct: 164 I 162
>TC14242 homologue to UP|PSAF_SPIOL (P12355) Photosystem I reaction centre
subunit III, chloroplast precursor (Light-harvesting
complex I 17 kDa protein) (PSI-F), partial (28%)
Length = 558
Score = 28.9 bits (63), Expect = 0.41
Identities = 15/45 (33%), Positives = 23/45 (50%)
Frame = -1
Query: 7 TPLTLFRQKHPTTKLPYPSQLNTRPRLGSGSHPSSPSLTFTVRSS 51
T L L + P++ LP P + T +HPSSP L T+ ++
Sbjct: 417 TSLVLQESRAPSSSLPLPPSIFT-------THPSSPCLIITINNN 304
>AV419985
Length = 408
Score = 28.1 bits (61), Expect = 0.70
Identities = 17/38 (44%), Positives = 19/38 (49%), Gaps = 6/38 (15%)
Frame = +1
Query: 8 PLTLFRQKHP----TTKLPYPSQLNTRPRLG--SGSHP 39
P L R +HP T +P P L RPR G GSHP
Sbjct: 178 PRHLARPRHPQWAPTPPMPLPPHLAGRPRSGLLGGSHP 291
>TC7804 similar to UP|Q9M600 (Q9M600) Chlorophyll a/b binding protein
precursor, partial (17%)
Length = 411
Score = 27.3 bits (59), Expect = 1.2
Identities = 13/37 (35%), Positives = 20/37 (53%)
Frame = -2
Query: 16 HPTTKLPYPSQLNTRPRLGSGSHPSSPSLTFTVRSSY 52
H K+ +Q TRP+ + +H S PSL+F S +
Sbjct: 350 HTAGKVHGNNQYLTRPKFKTQTHTSHPSLSFLCNSHF 240
>AW719654
Length = 491
Score = 26.6 bits (57), Expect = 2.0
Identities = 15/46 (32%), Positives = 23/46 (49%)
Frame = +1
Query: 35 SGSHPSSPSLTFTVRSSYKVVIEHEGKTTQLEVEPDETILSKALDS 80
+G HP SPSLT S + ++I T ++ P T+ S A +
Sbjct: 85 NGPHPKSPSLT----SCHSIIINLPTLTLLHQIAPPSTMASIAFST 210
>BF177505
Length = 478
Score = 26.6 bits (57), Expect = 2.0
Identities = 18/38 (47%), Positives = 22/38 (57%), Gaps = 3/38 (7%)
Frame = -2
Query: 20 KLPYPS---QLNTRPRLGSGSHPSSPSLTFTVRSSYKV 54
+ P+PS QLN RLGS SS SL T +SSY +
Sbjct: 252 RTPFPSKSMQLNQNSRLGSDDLSSSDSLK*T-KSSYPI 142
>BP055490
Length = 547
Score = 26.2 bits (56), Expect = 2.7
Identities = 16/33 (48%), Positives = 20/33 (60%), Gaps = 2/33 (6%)
Frame = -1
Query: 17 PTTKLPYPS--QLNTRPRLGSGSHPSSPSLTFT 47
P+T PS L T P + +GS SSPSLTF+
Sbjct: 358 PSTYSSNPSIAHLFTAPSVQNGSLQSSPSLTFS 260
>TC9100 similar to UP|Q8W544 (Q8W544) NADH-ubiquinone oxidoreductase
(Fragment), partial (97%)
Length = 712
Score = 26.2 bits (56), Expect = 2.7
Identities = 13/45 (28%), Positives = 24/45 (52%)
Frame = +1
Query: 98 ARLISGTVDQSDGMLSDDVVERGYALLCASYPRSDCHIRVIPEDE 142
AR + + ++ G++ DVV +L Y ++ I+ +PEDE
Sbjct: 49 ARPLMAKMKETTGIVGLDVVPNAREVLIGLYNKTLTEIQKVPEDE 183
>TC9676 similar to PIR|S71223|S71223 xyloglucan endo-1,4-beta-D-glucanase
XTR-4 - Arabidopsis thaliana (fragment)
{Arabidopsis thaliana;} , partial (51%)
Length = 716
Score = 26.2 bits (56), Expect = 2.7
Identities = 13/35 (37%), Positives = 17/35 (48%)
Frame = +2
Query: 17 PTTKLPYPSQLNTRPRLGSGSHPSSPSLTFTVRSS 51
P+T PYPS + P LG + + P T SS
Sbjct: 158 PSTSPPYPSTMPIHPSLGMATLSAPPMATVFNSSS 262
>TC9418 similar to UP|Q94JQ3 (Q94JQ3) AT4g32520/F8B4_220 (Serine
hydroxymethyltransferase) (Serine methylase) (Glycine
hydroxymethyltransferase) (SHMT) , partial (60%)
Length = 1231
Score = 26.2 bits (56), Expect = 2.7
Identities = 19/68 (27%), Positives = 29/68 (41%), Gaps = 18/68 (26%)
Frame = +3
Query: 81 GLDVPHDCKLGVCMTCPARLISGT----------VDQSDGMLSDDVVERGYALL------ 124
GLD+PH L P R +S T +D+S G + D++E+ L
Sbjct: 630 GLDLPHGGHLSHGFMTPKRRVSATSIYFESMPYRLDESTGFIDYDMLEKTAILFRPKLII 809
Query: 125 --CASYPR 130
++YPR
Sbjct: 810 AGASAYPR 833
>AV768401
Length = 363
Score = 25.4 bits (54), Expect = 4.5
Identities = 21/68 (30%), Positives = 30/68 (43%), Gaps = 3/68 (4%)
Frame = +1
Query: 80 SGLDVPHDCKLGVCMTCPARLISGTVDQSDG---MLSDDVVERGYALLCASYPRSDCHIR 136
S ++V D C T A L+ +D+SD ++ D + Y S PRS HI
Sbjct: 118 SSIEVDQDHPSASCYTLHANLLG--LDESDPKTWLILD--FHKPYIASRCSRPRSHIHIS 285
Query: 137 VIPEDELL 144
+P D L
Sbjct: 286 NLPRDNKL 309
>BP048304
Length = 398
Score = 25.4 bits (54), Expect = 4.5
Identities = 12/31 (38%), Positives = 18/31 (57%)
Frame = +1
Query: 16 HPTTKLPYPSQLNTRPRLGSGSHPSSPSLTF 46
H K+ +Q TRP+ + +H S PSL+F
Sbjct: 43 HTQGKVHGNNQYLTRPKFKTQTHTSHPSLSF 135
>TC12300 weakly similar to UP|Q8BKL0 (Q8BKL0) Splicing factor, partial
(18%)
Length = 563
Score = 25.0 bits (53), Expect = 5.9
Identities = 12/24 (50%), Positives = 13/24 (54%)
Frame = +1
Query: 1 MATLQFTPLTLFRQKHPTTKLPYP 24
MA L PLTL T +LPYP
Sbjct: 58 MALLALDPLTLHHPSVMTKELPYP 129
>TC12700 similar to UP|P93390 (P93390) Phosphate/phosphoenolpyruvate
translocator, partial (9%)
Length = 442
Score = 24.6 bits (52), Expect = 7.7
Identities = 16/48 (33%), Positives = 21/48 (43%), Gaps = 4/48 (8%)
Frame = +3
Query: 2 ATLQFTPLTL----FRQKHPTTKLPYPSQLNTRPRLGSGSHPSSPSLT 45
A +Q PL L F T ++P PS + P + P PSLT
Sbjct: 213 AQIQMAPLLLPHPPFLADPGTLRIPLPSSSDLSPLIHRRLKPLPPSLT 356
>TC16150 similar to GB|AAO63328.1|28950809|BT005264 At4g25840 {Arabidopsis
thaliana;}, partial (90%)
Length = 1065
Score = 24.6 bits (52), Expect = 7.7
Identities = 18/47 (38%), Positives = 23/47 (48%), Gaps = 8/47 (17%)
Frame = +3
Query: 13 RQKHPTTKLPYPSQLNTR------PRLGSGSHPSSPS--LTFTVRSS 51
RQKH T +P+P L + P S P SP+ LT+TV S
Sbjct: 81 RQKHCTLSVPFPRFLQNQTKWRIPPAPPSLPEPQSPTSFLTWTVSFS 221
>TC12781 weakly similar to UP|Q940Z5 (Q940Z5) AT5g39050/MXF12_60, partial
(12%)
Length = 463
Score = 24.6 bits (52), Expect = 7.7
Identities = 9/24 (37%), Positives = 15/24 (62%)
Frame = -3
Query: 8 PLTLFRQKHPTTKLPYPSQLNTRP 31
P+T + +PT+ P+PS L+ P
Sbjct: 194 PITCLSRTNPTSTPPFPSLLSAMP 123
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.133 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,624,454
Number of Sequences: 28460
Number of extensions: 32536
Number of successful extensions: 260
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 244
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 259
length of query: 153
length of database: 4,897,600
effective HSP length: 82
effective length of query: 71
effective length of database: 2,563,880
effective search space: 182035480
effective search space used: 182035480
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)
Medicago: description of AC135320.16