BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135319.9 + phase: 0 /pseudo
(734 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19395 63 1e-10
TC17416 similar to UP|KADD_ARATH (Q9FIJ7) Probable adenylate kin... 31 0.81
TC16757 weakly similar to UP|Q9SBQ2 (Q9SBQ2) Anthocyanin 5-O-glu... 31 0.81
TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly pr... 30 1.4
TC12780 30 1.4
TC9815 weakly similar to UP|Q9FEA1 (Q9FEA1) Anthocyanin 1, parti... 29 3.1
BI418736 29 3.1
TC10480 29 3.1
CB828882 28 4.0
TC10836 similar to GB|AAL60196.1|18139887|AF441079 O-linked N-ac... 28 5.2
CB827534 28 5.2
AV766686 28 6.8
TC8153 weakly similar to UP|Q8VAJ3 (Q8VAJ3) Wsv416 (WSSV475), pa... 28 6.8
TC14858 homologue to UP|NUIM_SOLTU (P80269) NADH-ubiquinone oxid... 27 8.9
BP055778 27 8.9
TC15493 similar to UP|Q9I9B8 (Q9I9B8) Non-Canonical ubiquitin co... 27 8.9
TC19852 similar to UP|Q9FR37 (Q9FR37) Amidase (At1g08980/F7G19_1... 27 8.9
TC9502 similar to UP|Q8LG88 (Q8LG88) Sodium-dicarboxylate cotran... 27 8.9
BP083037 27 8.9
>TC19395
Length = 509
Score = 63.2 bits (152), Expect = 1e-10
Identities = 29/36 (80%), Positives = 31/36 (85%)
Frame = +3
Query: 699 AEADLLYSLITKAKFENNRYQKPKKKTKFSTQHVDW 734
AE DLLYSLI KAKFENNRYQ PKK+ + STQHVDW
Sbjct: 15 AEQDLLYSLIIKAKFENNRYQTPKKQPQTSTQHVDW 122
>TC17416 similar to UP|KADD_ARATH (Q9FIJ7) Probable adenylate kinase 2,
chloroplast precursor (ATP-AMP transphosphorylase) ,
partial (36%)
Length = 537
Score = 30.8 bits (68), Expect = 0.81
Identities = 18/48 (37%), Positives = 24/48 (49%), Gaps = 6/48 (12%)
Frame = +1
Query: 334 CSFRSKWIRRRSYPNIY------ENGGAGDGG*RQHGFCCSWGIWCRH 375
C FR ++ R YP + +GG G+GG H F CS G W R+
Sbjct: 148 CCFRCPFVLRCLYPLSFPIYACQSSGGGGEGGSPCHDFRCS-GFW*RN 288
>TC16757 weakly similar to UP|Q9SBQ2 (Q9SBQ2) Anthocyanin
5-O-glucosyltransferase, partial (19%)
Length = 638
Score = 30.8 bits (68), Expect = 0.81
Identities = 17/45 (37%), Positives = 22/45 (48%)
Frame = -1
Query: 28 TFPIPISFNHKISPQPRRFNISSYPLRQRPKPHSRFFHPCPNHQT 72
+F +P H SP P N S +PL +P PHSR+ P T
Sbjct: 215 SFSLPRLSLHSPSPHP---NTS*FPLLPQPHPHSRYDPSSPQSST 90
>TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly protein
1-like protein 3, partial (81%)
Length = 1298
Score = 30.0 bits (66), Expect = 1.4
Identities = 14/40 (35%), Positives = 23/40 (57%)
Frame = -1
Query: 31 IPISFNHKISPQPRRFNISSYPLRQRPKPHSRFFHPCPNH 70
+P++ +++ P R ++ SS PL + P H R HP P H
Sbjct: 938 VPLADLYQVGPCYRSYSCSSLPLPRHP--HHRHRHPLPLH 825
>TC12780
Length = 487
Score = 30.0 bits (66), Expect = 1.4
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = -3
Query: 17 FSKSTLQHKATTFPIPISFNHKISPQPRRFNISSYPLRQRP 57
++KSTL + + +P + H I P P F + S P P
Sbjct: 398 YAKSTLLRQRESISVPFAVEHSICPPPPWFTLRSSPSHPLP 276
>TC9815 weakly similar to UP|Q9FEA1 (Q9FEA1) Anthocyanin 1, partial (11%)
Length = 634
Score = 28.9 bits (63), Expect = 3.1
Identities = 24/65 (36%), Positives = 32/65 (48%), Gaps = 4/65 (6%)
Frame = -3
Query: 16 RFSKSTLQHKATTFP---IPISFNHKISPQPRRFNIS-SYPLRQRPKPHSRFFHPCPNHQ 71
RF S QH TT+P + H + +RF + S + Q + +SR FHPC Q
Sbjct: 416 RFLVSLFQH--TTYPFFWVLTGDFHMLITTSKRF*LDVSVIVCQVVRQYSRLFHPC-TFQ 246
Query: 72 TTTFL 76
T TFL
Sbjct: 245 TKTFL 231
>BI418736
Length = 544
Score = 28.9 bits (63), Expect = 3.1
Identities = 10/15 (66%), Positives = 11/15 (72%)
Frame = +3
Query: 55 QRPKPHSRFFHPCPN 69
QRP PH+ F PCPN
Sbjct: 159 QRPLPHTVFLRPCPN 203
>TC10480
Length = 842
Score = 28.9 bits (63), Expect = 3.1
Identities = 13/36 (36%), Positives = 18/36 (49%)
Frame = -2
Query: 50 SYPLRQRPKPHSRFFHPCPNHQTTTFLRF*WLPTEC 85
S+P +++ KPH H CPN+ W TEC
Sbjct: 433 SFPFKRKLKPHHYPRHRCPNN---------WCSTEC 353
>CB828882
Length = 513
Score = 28.5 bits (62), Expect = 4.0
Identities = 21/65 (32%), Positives = 30/65 (45%), Gaps = 4/65 (6%)
Frame = -2
Query: 10 NLPFMGRFSKSTLQHKATTFPIPISFNHKIS----PQPRRFNISSYPLRQRPKPHSRFFH 65
N+PF+ + L HKA FP + H S P+ R+ + +S P Q + H H
Sbjct: 434 NIPFL*Q----NLHHKAENFPCNLPTLHHQSPPRLPKERQQHHNSDPRHQNRRNH---HH 276
Query: 66 PCPNH 70
C NH
Sbjct: 275 QCCNH 261
>TC10836 similar to GB|AAL60196.1|18139887|AF441079 O-linked N-acetyl
glucosamine transferase {Arabidopsis thaliana;} ,
partial (16%)
Length = 833
Score = 28.1 bits (61), Expect = 5.2
Identities = 26/79 (32%), Positives = 30/79 (37%)
Frame = +3
Query: 17 FSKSTLQHKATTFPIPISFNHKISPQPRRFNISSYPLRQRPKPHSRFFHPCPNHQTTTFL 76
FS LQ PIP +F+ + P P + SS R P S TTT
Sbjct: 66 FSSDLLQPPQPPLPIPFAFHDYLLPPPLNWFRSSL---SRLPP*SPCRETTTATSTTTTC 236
Query: 77 RF*WLPTECPFYLELSAFN 95
RF LPT F A N
Sbjct: 237 RFLVLPTLLGFSSPRIALN 293
>CB827534
Length = 458
Score = 28.1 bits (61), Expect = 5.2
Identities = 15/46 (32%), Positives = 24/46 (51%)
Frame = +2
Query: 12 PFMGRFSKSTLQHKATTFPIPISFNHKISPQPRRFNISSYPLRQRP 57
PF +F S+L +K +FP ++P P +I S P+ +RP
Sbjct: 8 PFFFQFGLSSLSNKQCSFP--------LAPHPNPSSIYSSPVSKRP 121
>AV766686
Length = 536
Score = 27.7 bits (60), Expect = 6.8
Identities = 15/32 (46%), Positives = 17/32 (52%), Gaps = 5/32 (15%)
Frame = +1
Query: 341 IRRRSYPNIYENGGAGDGG*R-----QHGFCC 367
I RRS P GG+G G R +HGFCC
Sbjct: 328 IGRRS*PEKAPEGGSGGGRQRRNDDPEHGFCC 423
>TC8153 weakly similar to UP|Q8VAJ3 (Q8VAJ3) Wsv416 (WSSV475), partial
(18%)
Length = 476
Score = 27.7 bits (60), Expect = 6.8
Identities = 21/70 (30%), Positives = 30/70 (42%)
Frame = +1
Query: 9 TNLPFMGRFSKSTLQHKATTFPIPISFNHKISPQPRRFNISSYPLRQRPKPHSRFFHPCP 68
TN+ ++ + H + PI + SP P ++ P P S FF P
Sbjct: 91 TNITYVSSLCSTHAHHSS---PIITNIFFFFSPPPYFSQVAV------PTPCSCFFFPM- 240
Query: 69 NHQTTTFLRF 78
NH+TTTF F
Sbjct: 241 NHETTTFTSF 270
>TC14858 homologue to UP|NUIM_SOLTU (P80269) NADH-ubiquinone oxidoreductase
23 kDa subunit, mitochondrial precursor (Complex
I-23KD) (CI-23KD) (Complex I-28.5KD) (CI-28.5KD) ,
partial (93%)
Length = 1106
Score = 27.3 bits (59), Expect = 8.9
Identities = 13/28 (46%), Positives = 15/28 (53%)
Frame = -2
Query: 73 TTFLRF*WLPTECPFYLELSAFNVFKMW 100
TTFL W+P+EC F E A F W
Sbjct: 436 TTFLSC-WIPSECMFTAETRAQRAFLKW 356
>BP055778
Length = 574
Score = 27.3 bits (59), Expect = 8.9
Identities = 9/18 (50%), Positives = 10/18 (55%)
Frame = +1
Query: 527 AWWCFSLASIAWCL*HTW 544
AWWC A + W *H W
Sbjct: 196 AWWCEKRAEVPWHG*HDW 249
>TC15493 similar to UP|Q9I9B8 (Q9I9B8) Non-Canonical ubiquitin conjugating
enzyme 1 (NCUBE1) (Ubiquitin-conjugating enzyme E2)
(Ubiquitin-protein ligase) (Ubiquitin carrier protein) ,
partial (35%)
Length = 730
Score = 27.3 bits (59), Expect = 8.9
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = -2
Query: 164 GL*WLEIRNLYQCNTNDPWSGVCWWL*ERDYCRECS 199
G *WL +L C+ +P + WW *++ RE S
Sbjct: 579 GT*WLRRASLLSCSLMNPMHHLGWWA*KQSGQREQS 472
>TC19852 similar to UP|Q9FR37 (Q9FR37) Amidase (At1g08980/F7G19_15), partial
(17%)
Length = 571
Score = 27.3 bits (59), Expect = 8.9
Identities = 10/24 (41%), Positives = 14/24 (57%)
Frame = +2
Query: 366 CCSWGIWCRHLFGSWKADTLFNHK 389
C SWG+WC H S ++ T +K
Sbjct: 143 CSSWGLWCSHDPHSSRSSTKTTNK 214
>TC9502 similar to UP|Q8LG88 (Q8LG88) Sodium-dicarboxylate
cotransporter-like, partial (42%)
Length = 1253
Score = 27.3 bits (59), Expect = 8.9
Identities = 16/50 (32%), Positives = 21/50 (42%), Gaps = 8/50 (16%)
Frame = +1
Query: 363 HGFCCSWGIWCRHLFGSWKADTLFNHKKEFL*KSF--------CW*WTCK 404
H FC G+ C W D + + KK * S+ CW*W C+
Sbjct: 22 HVFC*EDGVVCL-----WVTDRIMDDKKNHR*HSWMGVFLSWPCW*WKCQ 156
>BP083037
Length = 289
Score = 27.3 bits (59), Expect = 8.9
Identities = 8/15 (53%), Positives = 11/15 (73%)
Frame = -3
Query: 361 RQHGFCCSWGIWCRH 375
++HGF C+W IW H
Sbjct: 185 QRHGFSCTWRIWIIH 141
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.365 0.163 0.692
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,578,645
Number of Sequences: 28460
Number of extensions: 302715
Number of successful extensions: 5049
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 2884
Number of HSP's successfully gapped in prelim test: 193
Number of HSP's that attempted gapping in prelim test: 2031
Number of HSP's gapped (non-prelim): 3333
length of query: 734
length of database: 4,897,600
effective HSP length: 97
effective length of query: 637
effective length of database: 2,136,980
effective search space: 1361256260
effective search space used: 1361256260
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 36 (21.6 bits)
S2: 59 (27.3 bits)
Medicago: description of AC135319.9