BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135319.4 - phase: 0
(581 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP069214 89 2e-18
TC19596 81 5e-16
BP085058 74 5e-14
AV767121 74 7e-14
BP076564 69 3e-12
BP053073 68 5e-12
AV780705 64 9e-11
BP060613 63 2e-10
BE122611 53 1e-09
AV411435 59 2e-09
BP068806 57 6e-09
AV780445 39 9e-09
BP085925 56 1e-08
TC11323 54 7e-08
AV780221 51 5e-07
BP056414 50 8e-07
BP046627 50 8e-07
CB826728 47 9e-06
AV426428 44 9e-05
AV780250 42 4e-04
>BP069214
Length = 545
Score = 89.0 bits (219), Expect = 2e-18
Identities = 52/117 (44%), Positives = 69/117 (58%), Gaps = 1/117 (0%)
Frame = -2
Query: 465 DILLAELTAIHQGLKLVTDLNVNEIMCYSDSLLAVNLIANDTPRYHIYAALIQNIKDLLN 524
+I EL A+ GL LV D +++C SDS A+ L+ P H YAALI +IKDLL+
Sbjct: 409 EITNMELLAMWHGLDLVWDRGFRQVICQSDSTEALALVLGVPPPRHRYAALIWSIKDLLS 230
Query: 525 -DRNISLHHTLREGNQCADFFAKFGANSDAALVVHQSPPADLLPLLRADALGIYFVR 580
+ +S+ H LREGN CADF AK+GA D LV+ Q P + LL D++ F R
Sbjct: 229 REWEVSMTHMLREGNSCADFLAKYGAMQDEPLVLPQEPLPGMTQLLFWDSVEFLFER 59
>TC19596
Length = 499
Score = 80.9 bits (198), Expect = 5e-16
Identities = 53/156 (33%), Positives = 85/156 (53%), Gaps = 2/156 (1%)
Frame = +1
Query: 426 LNVDGSCIGTPPRTGYGGVLRNSAGLFISGF-SGLIPNSADILLAELTAIHQGLKLVTDL 484
++ DG+ R G GG++R++ G +ISGF +G + D L AE+ A+ GL L+ +
Sbjct: 7 MDSDGAFKHDDDRMGMGGIVRDAHGAWISGFYAGSL--GGDALRAEIAALKHGLTLLWNA 180
Query: 485 NVNEIMCYSDSLLAVNLIANDTPRYHIYAALIQNIKDLLN-DRNISLHHTLREGNQCADF 543
+V C D L V + ND ++H A+ + +I+ LL+ D ++L + RE N AD
Sbjct: 181 HVRRATCEVDCLDIVEALENDRYQFHALASELLDIRLLLDRDWTVTLAYVPREANAAADC 360
Query: 544 FAKFGANSDAALVVHQSPPADLLPLLRADALGIYFV 579
A GA+ L +SPP +L P+L D L + F+
Sbjct: 361 LAGLGASLLCPLTCLESPPQELQPILARDLLAL*FL 468
>BP085058
Length = 452
Score = 74.3 bits (181), Expect = 5e-14
Identities = 40/79 (50%), Positives = 49/79 (61%)
Frame = +2
Query: 3 TRLASWKNRLLNKTGRLTLANSVLTSIPSYFMQTNWLPQNICDSIDQTTRNFIWRGTTNK 62
+RLA K LLNK RLTLA VL++I Y MQ NWLPQ+ICD I R+F+W
Sbjct: 209 SRLAC*KAYLLNKPARLTLAKPVLSAISMYVMQLNWLPQSICDQICTVVRHFVWENV--N 382
Query: 63 GIHLVNWKKISLPKNIGGL 81
GI LV W+ I K++ GL
Sbjct: 383 GIPLVAWETIQSRKSMVGL 439
>AV767121
Length = 525
Score = 73.9 bits (180), Expect = 7e-14
Identities = 50/172 (29%), Positives = 78/172 (45%), Gaps = 10/172 (5%)
Frame = +2
Query: 5 LASWKNRLLNKTGRLTLANSVLTSIPSYFMQTNWLPQNICDSIDQTTRNFIWRGTTNKG- 63
L+ WK + ++ GR+ L SVLT++P +F+ LP + S + RNF+W G+ N+
Sbjct: 2 LSKWKQKTVSFGGRIFLIQSVLTALPLFFLSFFKLPIGVGKSCVRLMRNFLWGGSENENK 181
Query: 64 IHLVNWKKISLPKNIGGLSIRAAREANTSLLGKLVWDMVQSSNKLW---VSLLSNKYTGG 120
I V W + PK +GGL I+ N +LLGK W + + LW + + Y+ G
Sbjct: 182 IA*VKWTDVCKPKELGGLGIKDLFTFNKALLGK*RWRYLTEPDSLWRRVIEAQPDHYSCG 361
Query: 121 HNTLYASANNRSSPTWSSIIR-----AKNILKDGYTWRIGSG-SSSFWFSNW 166
S W+ I+ A G +G G + FW +W
Sbjct: 362 ------------SSWWNDILSLCPEDADGWFSSGLKKLVGEGDQTKFWSEDW 481
>BP076564
Length = 371
Score = 68.6 bits (166), Expect = 3e-12
Identities = 32/104 (30%), Positives = 55/104 (52%)
Frame = +2
Query: 269 LKVPEKYKFLIWLACQNAVPTLSLLYHRSMAPSPICTRCGEEDETFMHCVRDCCFSKDIW 328
LKV EK KF++ L A+P + + SMA + C RC + +HC++DC S+++W
Sbjct: 56 LKVVEKCKFMVGLGLHQALPINARRHACSMAATGGCGRCSAAVKDILHCLQDCPHSREVW 235
Query: 329 QKIGFTSNDFVTTATTYDWIKLGISGSRSSIFLAGLWWVWRHRN 372
++G +S ++ + W+ + + LWW+WR RN
Sbjct: 236 NRLGCSSFGGFLSSDGWSWLLSIMKQPNVEKLIYILWWIWR*RN 367
>BP053073
Length = 456
Score = 67.8 bits (164), Expect = 5e-12
Identities = 42/118 (35%), Positives = 61/118 (51%), Gaps = 1/118 (0%)
Frame = -2
Query: 464 ADILLAELTAIHQGLKLVTDLNVNEIMCYSDSLLAVNLIANDTPRYHIYAALIQNIKDLL 523
++IL EL + GL+L V ++ ++DS LA++L+ R+H YAA++ I DLL
Sbjct: 434 SEILKXELLXMLYGLQL*WSKGVRRMLVFTDSQLALSLVQLGCDRFHQYAAIVGMIHDLL 255
Query: 524 N-DRNISLHHTLREGNQCADFFAKFGANSDAALVVHQSPPADLLPLLRADALGIYFVR 580
D ++ LREGN D AK G L + + P LPLL D G F+R
Sbjct: 254 RFDWQVNFRLILREGNAVVDLLAKDGTRDSCRLKLLEDPLQAALPLLADDYCGTLFMR 81
>AV780705
Length = 524
Score = 63.5 bits (153), Expect = 9e-11
Identities = 32/119 (26%), Positives = 56/119 (46%), Gaps = 2/119 (1%)
Frame = -2
Query: 1 MQTRLASWKNRLLNKTGRLTLANSVLTSIPSYFMQTNWLPQNICDSIDQTTRNFIWRGTT 60
++ +L WK LLN+ G+ L +++ +IPSY M P+ C+S+ +F W+
Sbjct: 418 VKEKLEGWKETLLNQAGKEVLIKAIIQAIPSYAMTIVHFPKTFCNSLYALVADFWWKSQG 239
Query: 61 NKGIHLVN--WKKISLPKNIGGLSIRAAREANTSLLGKLVWDMVQSSNKLWVSLLSNKY 117
+ K L K GG+ R R N + L + W ++ + LWV ++ + Y
Sbjct: 238 EGAVEYTGAVGTK*PLSKEKGGVGFRDLRTQNLAFLARQAWRVLTNPEALWVRVMKSLY 62
>BP060613
Length = 378
Score = 62.8 bits (151), Expect = 2e-10
Identities = 29/104 (27%), Positives = 51/104 (48%)
Frame = +1
Query: 56 WRGTTNKGIHLVNWKKISLPKNIGGLSIRAAREANTSLLGKLVWDMVQSSNKLWVSLLSN 115
W +G+H W ++ K+ GGL + N +LL K W ++ + + LWV +L
Sbjct: 49 WSSKGTRGVHWRKWDLLTELKDEGGLGFKDFEIQNQALLAKQAWRILHNPDALWVQILKA 228
Query: 116 KYTGGHNTLYASANNRSSPTWSSIIRAKNILKDGYTWRIGSGSS 159
Y H+ L + +S WSS++ + +L W++GSG +
Sbjct: 229 LYFPHHDFLQTTKRTGASWVWSSLLHGRELLLKQGQWQLGSGET 360
>BE122611
Length = 357
Score = 53.1 bits (126), Expect(2) = 1e-09
Identities = 23/69 (33%), Positives = 38/69 (54%)
Frame = -3
Query: 227 DALIWSQNKNGTYTTKSGFNWLITSRVPAIEPILHAWSWIWRLKVPEKYKFLIWLACQNA 286
D ++W++ NG Y+ KS +N LI WS +W+ PEK +F +WL +NA
Sbjct: 331 DRILWTEESNGCYSAKSTYNLLIEDGGNESR----FWSLVWKANAPEKVRFFLWLVGRNA 164
Query: 287 VPTLSLLYH 295
+P+ + +H
Sbjct: 163 LPSNARRHH 137
Score = 26.2 bits (56), Expect(2) = 1e-09
Identities = 14/35 (40%), Positives = 16/35 (45%)
Frame = -2
Query: 294 YHRSMAPSPICTRCGEEDETFMHCVRDCCFSKDIW 328
YH +A S C RCG E H R C S +W
Sbjct: 137 YH--LASSAACIRCGAPSEDANHVFRWCPGSAWMW 39
>AV411435
Length = 426
Score = 59.3 bits (142), Expect = 2e-09
Identities = 30/110 (27%), Positives = 49/110 (44%), Gaps = 7/110 (6%)
Frame = +3
Query: 226 EDALIWSQNKNGTYTTKSGFNWLITSRV-------PAIEPILHAWSWIWRLKVPEKYKFL 278
ED L W +G Y+ KSG+ + ++ + P W IW +VP+K +
Sbjct: 36 EDRLFWPFIPSGEYSVKSGYRAVKATQFNLSNLASSSSHPSSFLWHTIWGAQVPKKVRSF 215
Query: 279 IWLACQNAVPTLSLLYHRSMAPSPICTRCGEEDETFMHCVRDCCFSKDIW 328
+W A NA+P L R+M C C + E H + C +++ +W
Sbjct: 216 LWRAANNAIPVKRNLKRRNMGRDDFCPICNKGPEDINHALLACEWTRAVW 365
>BP068806
Length = 418
Score = 57.4 bits (137), Expect = 6e-09
Identities = 25/90 (27%), Positives = 42/90 (45%)
Frame = +3
Query: 270 KVPEKYKFLIWLACQNAVPTLSLLYHRSMAPSPICTRCGEEDETFMHCVRDCCFSKDIWQ 329
+VPEK + +W Q A+ S + +A SP RC + E HC+RDC ++D+W
Sbjct: 147 EVPEKIRVWVWFIMQGAIQVNSFRFRCKLATSPAGPRCQHQFEDIWHCLRDCRIARDVWV 326
Query: 330 KIGFTSNDFVTTATTYDWIKLGISGSRSSI 359
++ + W+K G +S+
Sbjct: 327 RLNALGWPGFFVSNAQAWVKSQAQGPHASL 416
>AV780445
Length = 504
Score = 39.3 bits (90), Expect(2) = 9e-09
Identities = 26/93 (27%), Positives = 40/93 (42%)
Frame = +1
Query: 361 LAGLWWVWRHRNLMCLNNETLSLFRLCNNIISAADAANSAFNGEENPPHSDRLVKWNNRN 420
LAG+W VW+ RN M + S+ + D + +G+ P S +W
Sbjct: 226 LAGVW*VWKWRNNMVFEDSPWSVDEAWRKLGHEHDEFITFSDGDAGDPGSWLSSRWQPPR 405
Query: 421 HQDFILNVDGSCIGTPPRTGYGGVLRNSAGLFI 453
LNVDGS G GG++R+ G ++
Sbjct: 406 QGTVKLNVDGSGREQDRCMGAGGLIRDDQGKWL 504
Score = 37.4 bits (85), Expect(2) = 9e-09
Identities = 18/54 (33%), Positives = 26/54 (47%)
Frame = +2
Query: 306 RCGEEDETFMHCVRDCCFSKDIWQKIGFTSNDFVTTATTYDWIKLGISGSRSSI 359
RC +E HC+RDC S++IW ++G + W LG+ RS I
Sbjct: 65 RCPAPEEDI*HCLRDCPHSQEIWLRLG-----------AWAWTNLGVGDHRSWI 193
>BP085925
Length = 334
Score = 56.2 bits (134), Expect = 1e-08
Identities = 27/53 (50%), Positives = 37/53 (68%), Gaps = 1/53 (1%)
Frame = -2
Query: 510 HIYAALIQNIKDLLNDR-NISLHHTLREGNQCADFFAKFGANSDAALVVHQSP 561
H YA+L+ +I+DL++ ++ L+HTLREGN CADF K GA D A VV +P
Sbjct: 309 HAYASLVWDIQDLMSRSWHVVLNHTLREGNACADFLTKHGAGQDEAFVVIDNP 151
>TC11323
Length = 657
Score = 53.9 bits (128), Expect = 7e-08
Identities = 39/121 (32%), Positives = 59/121 (48%)
Frame = +2
Query: 415 KWNNRNHQDFILNVDGSCIGTPPRTGYGGVLRNSAGLFISGFSGLIPNSADILLAELTAI 474
+W H F LN DGS +G G GG+LR+ + FI GFS + A I+ L AI
Sbjct: 2 RWLKPVHGRFKLNFDGSSLGNRGNAGGGGLLRDGSSNFIFGFS-IFLAVAQIMKLSLCAI 178
Query: 475 HQGLKLVTDLNVNEIMCYSDSLLAVNLIANDTPRYHIYAALIQNIKDLLNDRNISLHHTL 534
+GL + L + I DS + V+ I + + + +K LL+ ++ S+HH
Sbjct: 179 LEGLLVCKPLGYDGIGIECDSNIVVSWIRSRSCQLWYLWDF*GPLKGLLDVKDYSIHHIF 358
Query: 535 R 535
R
Sbjct: 359 R 361
>AV780221
Length = 461
Score = 51.2 bits (121), Expect = 5e-07
Identities = 45/161 (27%), Positives = 69/161 (41%), Gaps = 10/161 (6%)
Frame = +3
Query: 19 LTLANSVLTSIPSYFMQTNWLPQN-ICDSIDQTTRNFIWRGTTN-KGIHLVNWKKISLPK 76
L L SVL ++P + + LP +++ F R K I V W + PK
Sbjct: 3 LCLIRSVLIALPLFHLPFFKLPTGGFYTNVNN**DPFFGREVEGGKKIAWVKWSTVCRPK 182
Query: 77 NIGGLSIRAAREANTSLLGKLVWDMVQSSNKLWVSLLSNKYTGGHNTLYASANNRSSPTW 136
+ GGL ++ N +LLGK W M++ + LW +L K Y +A +S+ +W
Sbjct: 183 DEGGLGVQNLGLFNKALLGKWRWRMLKERDGLWYKVLLIK--------YKNAIPQSASSW 338
Query: 137 SSIIRAKNILKDGYTW-------RIGSGSS-SFWFSNWNSL 169
+ + + G W RIG G+ FW NW L
Sbjct: 339 WNDLHSTCFEDGGGGWMQRGLCRRIGEGTEVKFWNENWLGL 461
>BP056414
Length = 606
Score = 50.4 bits (119), Expect = 8e-07
Identities = 44/170 (25%), Positives = 77/170 (44%), Gaps = 8/170 (4%)
Frame = -3
Query: 416 WNNRNHQDFILNVDGSCIGTPPRTGYGGVLRNSAGLFISGFSGLIPNSADIL------LA 469
W F +NVDG+ G + GGV+R++AG + GF+ S +L
Sbjct: 562 WTRPPEGWFKVNVDGALSGDL-QASCGGVVRDAAGSWSKGFA----RSLGVLRWHXAFFV 398
Query: 470 ELTAIHQGLKLVTDLNVNEIMCYSDSLLAVNLIAN-DTPRYHIYAALIQNIKDLLN-DRN 527
EL A+ + + ++ +++ SDS V L+ N + H Y + +I+ ++ +
Sbjct: 397 ELMAVQTAVDFIMSWDIPQVIIESDSQQVVELLQNFNASSPHQYGEIAWDIQQKISVHGS 218
Query: 528 ISLHHTLREGNQCADFFAKFGANSDAALVVHQSPPADLLPLLRADALGIY 577
I + T RE N AD+ AK G + +P + LL+ D + I+
Sbjct: 217 IVITSTPREANFLADYLAKVGLSLPFGTHSFDAPFGECHSLLQKDLMSIF 68
>BP046627
Length = 392
Score = 50.4 bits (119), Expect = 8e-07
Identities = 24/51 (47%), Positives = 39/51 (76%), Gaps = 1/51 (1%)
Frame = +1
Query: 489 IMCYSDSLLAVNLIANDTPRYHIYAALIQNIKDLLN-DRNISLHHTLREGN 538
++C SDSL A+NL+++ +P + + +LI +IKDLLN ++L+HTLR+GN
Sbjct: 238 LICQSDSLDAINLVSSASPLRYAFTSLIWDIKDLLNRPWQVALYHTLRKGN 390
>CB826728
Length = 509
Score = 47.0 bits (110), Expect = 9e-06
Identities = 27/98 (27%), Positives = 42/98 (42%), Gaps = 7/98 (7%)
Frame = +1
Query: 231 WSQNKNGTYTTKSGFNW-------LITSRVPAIEPILHAWSWIWRLKVPEKYKFLIWLAC 283
W + +G Y++KSG+ + L+ S PA W +W + K ++W AC
Sbjct: 214 WPGSSDGWYSSKSGYEFIRLENKSLLPSTSPAAAVPSLIWKTVWSASSLPRCKEVMWRAC 393
Query: 284 QNAVPTLSLLYHRSMAPSPICTRCGEEDETFMHCVRDC 321
+P S L R + P G EDE +H + C
Sbjct: 394 AGYLPVRSALKRRGLDVDPSSPWSGLEDEAEVHVLLTC 507
>AV426428
Length = 413
Score = 43.5 bits (101), Expect = 9e-05
Identities = 32/112 (28%), Positives = 49/112 (43%), Gaps = 8/112 (7%)
Frame = +3
Query: 280 WLACQNAVPTLSLLYHRSMAPSPICTRCGEEDETFMHCVRDCCFSKDIW--QKIGFTSN- 336
W AC +P + L+ R +A PIC RC +T H V C IW +GF N
Sbjct: 96 WRACLGVLPVKASLHARGLAEDPICPRCLAAPKTADHAVLFCP*VHPIWFASSLGFRLNQ 275
Query: 337 -----DFVTTATTYDWIKLGISGSRSSIFLAGLWWVWRHRNLMCLNNETLSL 383
+FV D++++ FL L+ +W +N +C T++L
Sbjct: 276 ECKMHEFVA-----DFMQVA-D*DTCGEFLTILYAIWTAQNELCFRYTTVTL 413
>AV780250
Length = 494
Score = 41.6 bits (96), Expect = 4e-04
Identities = 25/90 (27%), Positives = 38/90 (41%), Gaps = 3/90 (3%)
Frame = +3
Query: 227 DALIWSQNKNGTYTTKSGFNWL---ITSRVPAIEPILHAWSWIWRLKVPEKYKFLIWLAC 283
D L W NG YT+KSG+ + + +P+ L +W+ + + LI A
Sbjct: 174 DVLAWPHTSNGEYTSKSGYAVVRNKVVHALPSSSTALQVSPVVWKAPTLPRCRELIGRAF 353
Query: 284 QNAVPTLSLLYHRSMAPSPICTRCGEEDET 313
+PT S L R M +C C E+
Sbjct: 354 LKILPTRSALRARGMDVDELCPSCETHRES 443
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.135 0.432
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,269,106
Number of Sequences: 28460
Number of extensions: 227635
Number of successful extensions: 1280
Number of sequences better than 10.0: 87
Number of HSP's better than 10.0 without gapping: 1257
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1269
length of query: 581
length of database: 4,897,600
effective HSP length: 95
effective length of query: 486
effective length of database: 2,193,900
effective search space: 1066235400
effective search space used: 1066235400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC135319.4