Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC135234.5 - phase: 0 /pseudo
         (256 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

CB828260                                                               91  1e-19
BP066148                                                               32  0.082
TC7807 homologue to UP|Q946J9 (Q946J9) Aquaporin protein PIP1,1,...    28  2.0
TC7806 homologue to UP|Q946J9 (Q946J9) Aquaporin protein PIP1,1,...    28  2.0
TC17490 similar to GB|AAO64817.1|29028876|BT005882 At3g04570 {Ar...    27  3.4
TC14545 similar to UP|Q9FT78 (Q9FT78) P23 co-chaperone, partial ...    27  3.4
TC8882 homologue to UP|Q43715 (Q43715) Chloroplastic outer envel...    26  5.9
TC18433                                                                26  5.9
AV418845                                                               26  5.9
TC15820 similar to UP|Q9SWF9 (Q9SWF9) Zinc finger protein, parti...    26  7.7

>CB828260 
          Length = 557

 Score = 91.3 bits (225), Expect = 1e-19
 Identities = 54/131 (41%), Positives = 75/131 (57%), Gaps = 3/131 (2%)
 Frame = +3

Query: 105 KRPYLTTQSERSGIYKPPKTRKKPNL-EGIGSMKCNCPFRLKGFFDKD--TNDWWLAILC 161
           ++ Y+    E+ G Y P    + P+L EG  + K  CPFRLKG   K+    DW L ++ 
Sbjct: 174 RKAYVMLGCEKHGKYVP---YRDPDLVEGTRTQKTECPFRLKGRPMKNGTDRDWRLKVME 344

Query: 162 GMHNHDLEEKLQGHLIAGRLSAEEKKKVIDMTKSLTVPRNILTNLKENNKESVTTIKQVY 221
           G HNH+    L GH   GRL++EEK++V  M+KS  +PR +L  LKENN  ++TTI Q+Y
Sbjct: 345 GAHNHEPARSLLGHNFVGRLNSEEKEQVEKMSKSWVLPRKMLLTLKENNPLNLTTISQIY 524

Query: 222 NVRTRWCKGER 232
               R  K  R
Sbjct: 525 GACKRLRKSLR 557


>BP066148 
          Length = 477

 Score = 32.3 bits (72), Expect = 0.082
 Identities = 13/23 (56%), Positives = 17/23 (73%)
 Frame = +1

Query: 199 PRNILTNLKENNKESVTTIKQVY 221
           PR +L  LKENN  ++TTI Q+Y
Sbjct: 88  PRKMLLTLKENNPSNLTTISQIY 156


>TC7807 homologue to UP|Q946J9 (Q946J9) Aquaporin protein PIP1,1, partial
           (27%)
          Length = 1289

 Score = 27.7 bits (60), Expect = 2.0
 Identities = 10/17 (58%), Positives = 11/17 (63%)
 Frame = -2

Query: 71  TTNARWREREELLTWVR 87
           TTN RWR R  +  WVR
Sbjct: 250 TTNHRWRRRSRMCPWVR 200


>TC7806 homologue to UP|Q946J9 (Q946J9) Aquaporin protein PIP1,1,
          complete
          Length = 1413

 Score = 27.7 bits (60), Expect = 2.0
 Identities = 10/17 (58%), Positives = 11/17 (63%)
 Frame = +1

Query: 71 TTNARWREREELLTWVR 87
          TTN RWR R  +  WVR
Sbjct: 55 TTNHRWRRRSRMCPWVR 105


>TC17490 similar to GB|AAO64817.1|29028876|BT005882 At3g04570 {Arabidopsis
          thaliana;}, partial (9%)
          Length = 604

 Score = 26.9 bits (58), Expect = 3.4
 Identities = 13/23 (56%), Positives = 13/23 (56%)
 Frame = +1

Query: 7  DDDVEPKPAKTEASGEGSGDVKP 29
          DDD  P  A T ASG GSG   P
Sbjct: 67 DDDEGPSGAATAASGGGSGSSPP 135


>TC14545 similar to UP|Q9FT78 (Q9FT78) P23 co-chaperone, partial (46%)
          Length = 1127

 Score = 26.9 bits (58), Expect = 3.4
 Identities = 16/46 (34%), Positives = 22/46 (47%), Gaps = 5/46 (10%)
 Frame = +1

Query: 64  RDTASFFTTNARWRER-----EELLTWVRRQGVKAGFGVCIDKSVL 104
           R + SF T +   R +     E    W RRQ V  G G C++K+ L
Sbjct: 262 RSSLSFLTRSM*RRAKLILGCEAYSLWCRRQRVDGGKGCCVEKASL 399


>TC8882 homologue to UP|Q43715 (Q43715) Chloroplastic outer envelope
           membrane protein (OEP75) precursor (OEP75), partial
           (32%)
          Length = 1246

 Score = 26.2 bits (56), Expect = 5.9
 Identities = 19/68 (27%), Positives = 31/68 (44%), Gaps = 1/68 (1%)
 Frame = -2

Query: 150 KDTNDWWLAILCGMHNHDLEEKLQGHLIAGRLSAEEKKKVIDMTKSLTVPRNI-LTNLKE 208
           +D + WW      +H H LEE  Q  L+       E+ ++   TK+L    +I ++N   
Sbjct: 420 EDKHRWWWFTSPFLHRHQLEEACQSKLV-----TIEEWELAANTKALIYLEHISISNYSS 256

Query: 209 NNKESVTT 216
            +K  V T
Sbjct: 255 IHKTCVIT 232


>TC18433 
          Length = 428

 Score = 26.2 bits (56), Expect = 5.9
 Identities = 17/46 (36%), Positives = 23/46 (49%)
 Frame = -2

Query: 53  KRVPIGVVCEARDTASFFTTNARWREREELLTWVRRQGVKAGFGVC 98
           KRVP GVV  + D       +    E+EE   W++R+G   G  VC
Sbjct: 139 KRVPCGVVVTSGD-------DG*CEEKEEPW*WLQREGEMDGECVC 23


>AV418845 
          Length = 293

 Score = 26.2 bits (56), Expect = 5.9
 Identities = 13/28 (46%), Positives = 15/28 (53%)
 Frame = -2

Query: 2   VDNVADDDVEPKPAKTEASGEGSGDVKP 29
           V   A D VEP  A T + GEG G  +P
Sbjct: 127 VSESAIDVVEPCQASTNSGGEGGGGRRP 44


>TC15820 similar to UP|Q9SWF9 (Q9SWF9) Zinc finger protein, partial (18%)
          Length = 663

 Score = 25.8 bits (55), Expect = 7.7
 Identities = 11/20 (55%), Positives = 13/20 (65%)
 Frame = -1

Query: 8   DDVEPKPAKTEASGEGSGDV 27
           DD EPK  + EAS EG  D+
Sbjct: 237 DDDEPKRRRPEASAEGGADM 178


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.318    0.136    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,506,550
Number of Sequences: 28460
Number of extensions: 59816
Number of successful extensions: 319
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 318
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 318
length of query: 256
length of database: 4,897,600
effective HSP length: 88
effective length of query: 168
effective length of database: 2,393,120
effective search space: 402044160
effective search space used: 402044160
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)


Medicago: description of AC135234.5