BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135230.10 - phase: 0
(548 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC10040 weakly similar to UP|O23042 (O23042) YUP8H12.11 protein,... 86 1e-17
TC15630 78 4e-15
BP069537 46 1e-05
BP034655 35 0.024
BF177554 30 0.77
AV411437 30 0.77
TC18923 UP|CAE45594 (CAE45594) S-receptor kinase-like protein 1,... 30 0.77
TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%) 30 1.0
TC17497 UP|CAE45595 (CAE45595) S-receptor kinase-like protein 2,... 30 1.3
AV770043 29 1.7
TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly pr... 29 1.7
CB826709 29 1.7
TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (... 29 2.2
TC8868 similar to UP|AAR06294 (AAR06294) Adenylosuccinate syntha... 29 2.2
CB827158 28 2.9
TC14792 similar to UP|O73620 (O73620) Nuclear protein SDK2, part... 28 3.8
TC18708 similar to UP|Q93VJ8 (Q93VJ8) AT5g11200/F2I11_90 (AT5g11... 28 5.0
TC14791 similar to UP|Q9LJ04 (Q9LJ04) ESTs AU082210(C53655), par... 28 5.0
TC12439 similar to UP|Q9FMJ2 (Q9FMJ2) Ankyrin-like protein, part... 28 5.0
TC7885 similar to UP|RL4B_ARATH (Q9SF40) 60S ribosomal protein L... 28 5.0
>TC10040 weakly similar to UP|O23042 (O23042) YUP8H12.11 protein, partial
(8%)
Length = 786
Score = 86.3 bits (212), Expect = 1e-17
Identities = 45/104 (43%), Positives = 66/104 (63%), Gaps = 6/104 (5%)
Frame = +3
Query: 132 TELKHIVFGIAASSNLWNTRKEYIKIWWRPKQT-RGVVWL----DQRVSTQRNEGLPDIR 186
T L H+VFGIA+S + W RK+Y+K+WWR +G V+L D+ ++ +P +
Sbjct: 474 TTLDHLVFGIASSKSSWAKRKDYVKLWWRNNNAMKGCVFLDTLPDEDEDNDASDSVPPLC 653
Query: 187 ISDDTSKFRYTNRQGQRSALRISRVVTETLKL-GLKDVRWFVMG 229
+S+DTS+ R T R G SA+R++RVV ET+ L DVRW+V G
Sbjct: 654 VSEDTSR*RXTCRGGLPSAIRVARVVVETIALKNNSDVRWYVFG 785
>TC15630
Length = 758
Score = 77.8 bits (190), Expect = 4e-15
Identities = 32/72 (44%), Positives = 46/72 (63%)
Frame = +3
Query: 439 AFNTRPVAKHPCQKPFVYYMSKTHFDTASRQIVGVYSRDQTKSPFCRWRMESPEKITSIV 498
AFNTRPV+++PCQKPFV+Y SK ++ +Q V Y R + P CRW+M P + +
Sbjct: 15 AFNTRPVSRNPCQKPFVFYFSKAQQNSTLQQTVTEYDRHRVPHPECRWKMADPSSLEKVT 194
Query: 499 VTKRRDPLRWKK 510
V K+ DP W++
Sbjct: 195 VYKKPDPHLWER 230
>BP069537
Length = 496
Score = 46.2 bits (108), Expect = 1e-05
Identities = 20/31 (64%), Positives = 22/31 (70%)
Frame = +1
Query: 331 DVYGDLLGLLGAHPVAPLVSLHHLDVVQPIF 361
D+ GD GLL AHPV PL+SLHH D PIF
Sbjct: 403 DLRGDTFGLLAAHPVTPLLSLHHPDYTDPIF 495
>BP034655
Length = 517
Score = 35.4 bits (80), Expect = 0.024
Identities = 16/50 (32%), Positives = 29/50 (58%)
Frame = +1
Query: 61 HMTSYNISSTSDKNIEHKVLPIENKEQMQDETEKETEQDEDDFREGEGEE 110
H+T+ + + D E + E +E+ ++E E+E E+DE+D E E E+
Sbjct: 106 HLTAADFGLSDDDEEEEEEEEEEEEEEEEEEEEEEEEEDEEDDEEDEDED 255
>BF177554
Length = 246
Score = 30.4 bits (67), Expect = 0.77
Identities = 10/34 (29%), Positives = 24/34 (70%)
Frame = +1
Query: 83 ENKEQMQDETEKETEQDEDDFREGEGEEQENKSP 116
++++ ++E + +E++E++ EGE EE+E+ P
Sbjct: 22 QDEDSCEEEAGESSEEEEEEEEEGEEEEEEDDGP 123
>AV411437
Length = 417
Score = 30.4 bits (67), Expect = 0.77
Identities = 20/73 (27%), Positives = 38/73 (51%)
Frame = +2
Query: 85 KEQMQDETEKETEQDEDDFREGEGEEQENKSPIVVRLTPEQMAQRQDTELKHIVFGIAAS 144
+E + +E E+E E++E+D +GE E+ ++ +++ EL+ G + S
Sbjct: 41 QETIAEEEEEEEEEEEEDGVDGELEQFDDDE---TDSEDDELETEGGGELEDAQVGGSTS 211
Query: 145 SNLWNTRKEYIKI 157
S L RKE +K+
Sbjct: 212 SPLVEKRKERLKL 250
>TC18923 UP|CAE45594 (CAE45594) S-receptor kinase-like protein 1, complete
Length = 2061
Score = 30.4 bits (67), Expect = 0.77
Identities = 26/111 (23%), Positives = 50/111 (44%), Gaps = 7/111 (6%)
Frame = +1
Query: 119 VRLTPEQMAQRQDTELKHIVFGIAASSNLWNTRKEYIKIWWRPKQTRGVVWLDQRVSTQR 178
+ +T Q Q +T + A L N++++Y IW++ R +VW+ R + +
Sbjct: 28 ITVTQNQSIQDDETLVSAEGTFEAGFFGLGNSQRQYFGIWYKSISPRTIVWVANRDAPVQ 207
Query: 179 NEGLPDIRISD-------DTSKFRYTNRQGQRSALRISRVVTETLKLGLKD 222
N I+++D D SK + G R+A + + ++ L +KD
Sbjct: 208 N-STATIKLTDKGNLLILDGSKGIIWSSNGSRAAEKPYMQLLDSGNLVVKD 357
>TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%)
Length = 1008
Score = 30.0 bits (66), Expect = 1.0
Identities = 17/57 (29%), Positives = 26/57 (44%), Gaps = 4/57 (7%)
Frame = -2
Query: 81 PIENKEQMQDE----TEKETEQDEDDFREGEGEEQENKSPIVVRLTPEQMAQRQDTE 133
P EN E +DE E + + DEDD E +G E E + + ++ +D E
Sbjct: 446 PEENGEDEEDEDGEDQEDDDDDDEDDDEEDDGGEDEEEEGVEEEDNEDEEEDEEDEE 276
>TC17497 UP|CAE45595 (CAE45595) S-receptor kinase-like protein 2, complete
Length = 2946
Score = 29.6 bits (65), Expect = 1.3
Identities = 10/40 (25%), Positives = 21/40 (52%)
Frame = +1
Query: 149 NTRKEYIKIWWRPKQTRGVVWLDQRVSTQRNEGLPDIRIS 188
N + Y +W++ R +VW+ R + RN P ++++
Sbjct: 307 NPQHHYFGVWYKSISPRTIVWVANRDAPLRNSTAPTLKVT 426
>AV770043
Length = 532
Score = 29.3 bits (64), Expect = 1.7
Identities = 14/39 (35%), Positives = 20/39 (50%)
Frame = -3
Query: 260 ESHVQNIHFSYAMAYGGGGFAISYPLAVELATMQDRCIQ 298
+SHV+ HF++ AY SYPL L M + +Q
Sbjct: 350 QSHVRITHFAHTAAYAASKPPPSYPLPHHLPQMPQQTVQ 234
>TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly protein
1-like protein 3, partial (81%)
Length = 1298
Score = 29.3 bits (64), Expect = 1.7
Identities = 29/116 (25%), Positives = 49/116 (42%), Gaps = 25/116 (21%)
Frame = +2
Query: 39 FNNEQECPMTTTTLDDTTQDDVHMT---SYNISST-SDKNIEHKVLPI------------ 82
F N + P +DD +++ Y+I ST DK I H V
Sbjct: 593 FFNPPQVPEDDDDIDDDAVEELQNLMEHDYDIGSTIRDKIIPHAVSWFTGEAEQSDFEDI 772
Query: 83 --------ENKEQMQDETEKETEQDEDDFREGEGE-EQENKSPIVVRLTPEQMAQR 129
E++++ D+ E+E + D+DD + EGE + ++KS R +Q +R
Sbjct: 773 EEDDEDGDEDEDEDDDDDEEEEDDDDDDEDDEEGEGKSKSKSGSKARPGKDQPTER 940
>CB826709
Length = 432
Score = 29.3 bits (64), Expect = 1.7
Identities = 17/46 (36%), Positives = 24/46 (51%)
Frame = +3
Query: 86 EQMQDETEKETEQDEDDFREGEGEEQENKSPIVVRLTPEQMAQRQD 131
E+M D+ E EQ +DD +G+G E EN V E++A D
Sbjct: 300 EEMDDDVE---EQVDDDGVDGDGAEDENAEGSVEEHEYEELAAEAD 428
>TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (SPBC3D6.14C
protein) (Fragment), partial (15%)
Length = 543
Score = 28.9 bits (63), Expect = 2.2
Identities = 13/39 (33%), Positives = 25/39 (63%), Gaps = 3/39 (7%)
Frame = +3
Query: 78 KVLPIENKEQMQDETEKETEQ---DEDDFREGEGEEQEN 113
K + +N+E+ D+ E++ E D+DD + +GEE+E+
Sbjct: 231 KPVQTDNEEEEDDDEEEDDEDEDDDDDDDDDDDGEEEED 347
Score = 28.1 bits (61), Expect = 3.8
Identities = 9/31 (29%), Positives = 22/31 (70%)
Frame = +3
Query: 83 ENKEQMQDETEKETEQDEDDFREGEGEEQEN 113
E+ ++ +D+ +++ + D+DD +GE EE ++
Sbjct: 261 EDDDEEEDDEDEDDDDDDDDDDDGEEEEDDD 353
>TC8868 similar to UP|AAR06294 (AAR06294) Adenylosuccinate synthase ,
partial (62%)
Length = 1273
Score = 28.9 bits (63), Expect = 2.2
Identities = 14/37 (37%), Positives = 22/37 (58%)
Frame = -2
Query: 468 RQIVGVYSRDQTKSPFCRWRMESPEKITSIVVTKRRD 504
R++VG+ + Q K+ C+WR ESP+ +T R D
Sbjct: 1017 RKLVGINTSMQ-KNTTCQWRHESPDSFEYESITSRPD 910
>CB827158
Length = 213
Score = 28.5 bits (62), Expect = 2.9
Identities = 15/36 (41%), Positives = 23/36 (63%), Gaps = 4/36 (11%)
Frame = +3
Query: 396 FWSISVSWGFMVQILRGVLSPR----ELEMPSRTFL 427
F ++S SWGFM ++LR +L + +L +P TFL
Sbjct: 42 F*NVSCSWGFMRRVLRDLLPSKKKAYQLLLPVVTFL 149
>TC14792 similar to UP|O73620 (O73620) Nuclear protein SDK2, partial (5%)
Length = 492
Score = 28.1 bits (61), Expect = 3.8
Identities = 18/80 (22%), Positives = 36/80 (44%)
Frame = +2
Query: 46 PMTTTTLDDTTQDDVHMTSYNISSTSDKNIEHKVLPIENKEQMQDETEKETEQDEDDFRE 105
P+ D + + H N T+D + + + ++ + + QD+ ETE D+ +
Sbjct: 125 PVDVEPQDQDSVELDHQQPPNPEPTTDLDQSNNTMAVDEEPEEQDQ---ETE-DQPQIDD 292
Query: 106 GEGEEQENKSPIVVRLTPEQ 125
EEQ+ ++P P+Q
Sbjct: 293 AVEEEQDPEAPATTESEPQQ 352
>TC18708 similar to UP|Q93VJ8 (Q93VJ8) AT5g11200/F2I11_90
(AT5g11170/F2I11_60), partial (16%)
Length = 582
Score = 27.7 bits (60), Expect = 5.0
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = -3
Query: 24 ITIVLYILYSCNILLFNN 41
+++VLY+LYSC LF N
Sbjct: 181 VSVVLYLLYSCICFLFRN 128
>TC14791 similar to UP|Q9LJ04 (Q9LJ04) ESTs AU082210(C53655), partial (37%)
Length = 1779
Score = 27.7 bits (60), Expect = 5.0
Identities = 18/83 (21%), Positives = 37/83 (43%)
Frame = +2
Query: 46 PMTTTTLDDTTQDDVHMTSYNISSTSDKNIEHKVLPIENKEQMQDETEKETEQDEDDFRE 105
P+ D + + H N T+D + + + ++ + + QD+ ETE D+ +
Sbjct: 110 PVDVEPQDQDSVELDHQQPPNPVPTTDLDQSNNTMAVDEEPEEQDQ---ETE-DQPQIDD 277
Query: 106 GEGEEQENKSPIVVRLTPEQMAQ 128
EEQ+ ++P P+Q +
Sbjct: 278 AVEEEQDPEAPATTESEPQQQQE 346
>TC12439 similar to UP|Q9FMJ2 (Q9FMJ2) Ankyrin-like protein, partial (5%)
Length = 534
Score = 27.7 bits (60), Expect = 5.0
Identities = 22/96 (22%), Positives = 40/96 (40%), Gaps = 3/96 (3%)
Frame = +3
Query: 10 IHTGSRSITTWLILITIVLYI-LYSCNILLFNNEQECPMTTTTL--DDTTQDDVHMTSYN 66
I RS +++ +TIV+++ L + + + P+ + D TT D +
Sbjct: 138 IDNNKRSASSYCSTVTIVVFVALCLVGLWMLTSSSVVPVQNVDVSQDSTTNDVKEQSEVK 317
Query: 67 ISSTSDKNIEHKVLPIENKEQMQDETEKETEQDEDD 102
S S+ + KEQ D T+ + + EDD
Sbjct: 318 QQSESESEV---------KEQPTDTTDSKPRRFEDD 398
>TC7885 similar to UP|RL4B_ARATH (Q9SF40) 60S ribosomal protein L4-2 (L1),
complete
Length = 1554
Score = 27.7 bits (60), Expect = 5.0
Identities = 15/35 (42%), Positives = 16/35 (44%)
Frame = -1
Query: 148 WNTRKEYIKIWWRPKQTRGVVWLDQRVSTQRNEGL 182
WN R + WRP RG WL QR R GL
Sbjct: 216 WNGRN*RCQDGWRPS*HRGWEWLRQRRQPCRPPGL 112
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.136 0.416
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,077,535
Number of Sequences: 28460
Number of extensions: 138424
Number of successful extensions: 928
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 898
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 916
length of query: 548
length of database: 4,897,600
effective HSP length: 95
effective length of query: 453
effective length of database: 2,193,900
effective search space: 993836700
effective search space used: 993836700
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC135230.10