BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC134822.6 + phase: 0
(289 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14246 similar to UP|Q84LL6 (Q84LL6) Salt tolerance protein 5, ... 374 e-104
TC8259 similar to UP|HMGL_SOYBN (P26585) HMG1/2-like protein (SB... 36 0.009
TC11317 similar to UP|Q40363 (Q40363) NuM1 protein, partial (12%) 34 0.025
TC14264 similar to UP|Q84NF9 (Q84NF9) Histone H1 (Fragment), par... 33 0.057
TC14602 UP|Q8GS56 (Q8GS56) Phosphoenolpyruvate carboxylase kinas... 31 0.28
TC15487 similar to UP|O23144 (O23144) Proton pump interactor (AT... 30 0.37
CB829423 30 0.48
BP028571 28 1.4
TC7823 similar to UP|O24470 (O24470) Lipoxygenase , partial (96%) 28 1.8
BP067321 28 1.8
TC12189 similar to UP|Q9FKV6 (Q9FKV6) Eukaryotic translation ini... 28 1.8
AU251825 28 1.8
BG662181 28 2.4
TC17553 weakly similar to UP|AAP20176 (AAP20176) Kinase substrat... 28 2.4
TC9170 similar to UP|Q9FF36 (Q9FF36) Gb|AAF31027.1, partial (37%) 27 3.1
AV778023 27 3.1
TC14311 homologue to UP|Q9FFC0 (Q9FFC0) Histone H2B like protein... 27 3.1
TC19623 similar to PIR|T52371|T52371 homeobox protein HAT9 [impo... 27 3.1
TC18866 similar to UP|Q94A79 (Q94A79) AT3g49250/F2K15_110, parti... 27 3.1
BP072343 27 4.1
>TC14246 similar to UP|Q84LL6 (Q84LL6) Salt tolerance protein 5, partial
(57%)
Length = 1280
Score = 374 bits (960), Expect = e-104
Identities = 190/299 (63%), Positives = 236/299 (78%), Gaps = 11/299 (3%)
Frame = +1
Query: 1 MAIISDYQDDNQTKTSSSSS------SQPKPSKPIPFSSTFDPSNPTTFLEKVFDFIAKE 54
MAIISD+ ++ + +++ S SQ KPS F++TFD SNP FLE+VFDF+++E
Sbjct: 97 MAIISDFHEEEPQQNNAAPSKTDASPSQSKPSPSPTFAATFDRSNPAAFLERVFDFVSRE 276
Query: 55 STNFFDKDSAEKVVLSAVRAARVKKAKSVAAEKAKIAAKEKAKAAAEKKLNDEKSEAVTE 114
S +F ++AEK V S VRAAR K+ K E+ A++EKAKA EK+L + + + E
Sbjct: 277 S-DFLATEAAEKEVASLVRAAREKRKKK--REEELKASEEKAKA--EKRLKEAEKKPKAE 441
Query: 115 KIDEKAA-----PNQGNGMDLEKYSWTQTLQELNVNVPVPNGTKSGFVICEIKKNHLKVG 169
+ D+K PN+GNGMDLEKYSWTQTLQE+ VNVPVP+GTKS FV+CEIKKNHLKVG
Sbjct: 442 ENDKKEGTAAIVPNKGNGMDLEKYSWTQTLQEVTVNVPVPHGTKSRFVVCEIKKNHLKVG 621
Query: 170 LKGQPPIIDRELYKSIKPDECYWSIEDQNTVSILLTKHDQMDWWKCLVKGDPEINTQKVE 229
LKGQPPII+ E ++ +KPD+CYWSIEDQ+ +SILLTKHDQMDWWKCLVKGDPEI+TQKVE
Sbjct: 622 LKGQPPIIEGEFFRPVKPDDCYWSIEDQSALSILLTKHDQMDWWKCLVKGDPEIDTQKVE 801
Query: 230 PASSKLGDLDSETRMTVEKMMFDQRQKSMGLPTSEELQKEEMMKKFMSQHPNMDFSGAK 288
P +S+L DLD+ETR TVEKMMFDQRQKSMGLPTS+ELQK+E++KKFMS+HP MDFS AK
Sbjct: 802 PENSRLSDLDAETRQTVEKMMFDQRQKSMGLPTSDELQKQELLKKFMSEHPEMDFSRAK 978
>TC8259 similar to UP|HMGL_SOYBN (P26585) HMG1/2-like protein (SB11
protein), partial (92%)
Length = 942
Score = 35.8 bits (81), Expect = 0.009
Identities = 35/105 (33%), Positives = 54/105 (51%), Gaps = 8/105 (7%)
Frame = +2
Query: 24 KPSKPIPFSSTFDPSNPTTFLEKVFDFIAKESTNFFDKDSAEKVVLSAVRAARVKKAKSV 83
KP++ + DP+ P F F+ ++ F+K++ E +SAV A K KS+
Sbjct: 257 KPARGAKGKAAKDPNKPKRPPSAFFVFM-EDFRKIFNKENPENKAVSAVGKAAGAKWKSM 433
Query: 84 A-AEKAKIAAK-EKAKAAAEKKL---NDEKSE---AVTEKIDEKA 120
+ AEKA AK EK KA EK + N +++E A E+ EK+
Sbjct: 434 SEAEKAPYVAKAEKRKADYEKTMKAYNKKQAEGPAAADEEESEKS 568
>TC11317 similar to UP|Q40363 (Q40363) NuM1 protein, partial (12%)
Length = 547
Score = 34.3 bits (77), Expect = 0.025
Identities = 23/63 (36%), Positives = 33/63 (51%), Gaps = 3/63 (4%)
Frame = +1
Query: 63 SAEKVVLSAVRAARVKKAKSVAAEKAKIA---AKEKAKAAAEKKLNDEKSEAVTEKIDEK 119
+A K A RAA + K V+A+K KIA AK+K + +K + S+ + DEK
Sbjct: 139 AAPKSAKKAKRAAEDEVEKQVSAKKQKIAEVAAKQKKETKLQKVKKESSSDDDSSSEDEK 318
Query: 120 AAP 122
AP
Sbjct: 319 PAP 327
>TC14264 similar to UP|Q84NF9 (Q84NF9) Histone H1 (Fragment), partial (76%)
Length = 1277
Score = 33.1 bits (74), Expect = 0.057
Identities = 26/91 (28%), Positives = 36/91 (38%), Gaps = 2/91 (2%)
Frame = +2
Query: 61 KDSAEKVVLSAVRAARVKKAKSVAAEKAKIAAKEKAKAAAEKKLNDEKSEAVTEKIDEKA 120
K A+ +A A+ K A A K AK K AAA+ K K A
Sbjct: 530 KPKAKAKPAAAATKAKAKPAPKSKAAATKTTAKAKPAAAAKPKAKPAAKAKPAAKAKAVA 709
Query: 121 APNQGNGMDL--EKYSWTQTLQELNVNVPVP 149
AP + + + ++T +NVN PVP
Sbjct: 710 APAKAKASPAKPKAKAKSKTAPRMNVNPPVP 802
>TC14602 UP|Q8GS56 (Q8GS56) Phosphoenolpyruvate carboxylase kinase, complete
Length = 1256
Score = 30.8 bits (68), Expect = 0.28
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = +3
Query: 8 QDDNQTKTSSSSSSQPKPSKPIPFSSTFDPSNPT 41
+ D SS +S P P P P +S+ +PS+PT
Sbjct: 84 KSDAAVSAPSSDASTPSPPTPSPSNSSINPSSPT 185
>TC15487 similar to UP|O23144 (O23144) Proton pump interactor
(AT4g27500/F27G19_100), partial (16%)
Length = 1153
Score = 30.4 bits (67), Expect = 0.37
Identities = 19/45 (42%), Positives = 23/45 (50%), Gaps = 6/45 (13%)
Frame = +2
Query: 73 RAARVKKAKSVAAEKAKIAAKEKAKAA------AEKKLNDEKSEA 111
R + KAK K K+A K AKAA AEKKL D + +A
Sbjct: 455 REEEIAKAKQAWERKKKLAEKAAAKAALKAQKEAEKKLKDREKKA 589
>CB829423
Length = 383
Score = 30.0 bits (66), Expect = 0.48
Identities = 14/27 (51%), Positives = 15/27 (54%)
Frame = -3
Query: 17 SSSSSQPKPSKPIPFSSTFDPSNPTTF 43
S S QP P +PF S F PSN T F
Sbjct: 225 SPSQHQPHPLFVVPFQSRFQPSNITQF 145
>BP028571
Length = 487
Score = 28.5 bits (62), Expect = 1.4
Identities = 32/152 (21%), Positives = 65/152 (42%), Gaps = 6/152 (3%)
Frame = +2
Query: 79 KAKSVAAEKAKIAAKEKAKAAAEKKLNDEKSEAVTEKIDEKAAPNQGNGMDLEKYSWTQ- 137
K + A KAK++ +E+++ EKK +K + +K KA + ++ EK W +
Sbjct: 32 KGTTKATGKAKMSKEERSRLMEEKK--XQKEQEKLQKAALKAEAAELKKIEKEKQKWEKG 205
Query: 138 --TLQELNVNVP---VPNGTKSGFVICEIKKNHLKVGLKGQPPIIDRELYKSIKPDECYW 192
++ + + V +G+ G ++ + LK + P + SI W
Sbjct: 206 KFAIKSIVAKIDSKVVESGSIGGHLLTRFAEKGLKYQITSNP------ITGSI-----LW 352
Query: 193 SIEDQNTVSILLTKHDQMDWWKCLVKGDPEIN 224
S+E +S L T+ ++ + + + D N
Sbjct: 353 SMEVPEHISQLSTERVEIPYVLLVYEADKFCN 448
>TC7823 similar to UP|O24470 (O24470) Lipoxygenase , partial (96%)
Length = 2909
Score = 28.1 bits (61), Expect = 1.8
Identities = 16/37 (43%), Positives = 22/37 (59%), Gaps = 2/37 (5%)
Frame = +1
Query: 4 ISDYQDDNQTKTS--SSSSSQPKPSKPIPFSSTFDPS 38
IS+ Q+ + +KTS +SSSS PSK P + F S
Sbjct: 403 ISESQEHSTSKTSPRTSSSSSVSPSKTFPITEPFTSS 513
>BP067321
Length = 513
Score = 28.1 bits (61), Expect = 1.8
Identities = 13/24 (54%), Positives = 15/24 (62%)
Frame = -1
Query: 11 NQTKTSSSSSSQPKPSKPIPFSST 34
N+T SSSSS P S +PF ST
Sbjct: 489 NRTTEPSSSSSSPSRSSTVPFRST 418
>TC12189 similar to UP|Q9FKV6 (Q9FKV6) Eukaryotic translation initiation
factor 3 subunit 7, partial (30%)
Length = 658
Score = 28.1 bits (61), Expect = 1.8
Identities = 13/31 (41%), Positives = 15/31 (47%)
Frame = +1
Query: 12 QTKTSSSSSSQPKPSKPIPFSSTFDPSNPTT 42
Q T +S S+ P P P P SS P P T
Sbjct: 94 QPPTPTSLSTSPSPPSPAPTSSAGSPIGPAT 186
>AU251825
Length = 237
Score = 28.1 bits (61), Expect = 1.8
Identities = 19/55 (34%), Positives = 29/55 (52%), Gaps = 5/55 (9%)
Frame = +3
Query: 74 AARVKKAKSVAAEKAKIAAKEKAKAA-----AEKKLNDEKSEAVTEKIDEKAAPN 123
A ++ K AEK AA+EKA AA AEKK+ ++ A +K +++ N
Sbjct: 3 ALKMAKGDKKPAEKKPAAAEEKAAAAEKKPRAEKKIPAKEGGAAGDKKKKRSKKN 167
>BG662181
Length = 421
Score = 27.7 bits (60), Expect = 2.4
Identities = 17/61 (27%), Positives = 28/61 (45%)
Frame = +3
Query: 45 EKVFDFIAKESTNFFDKDSAEKVVLSAVRAARVKKAKSVAAEKAKIAAKEKAKAAAEKKL 104
EK D +AK + +K A + + AKS+AAE + + + + +A KK
Sbjct: 102 EKYNDLVAKHNAVMHGIAEVKKAAAKAGKRGHARFAKSLAAELSALRVEREREAKFLKKE 281
Query: 105 N 105
N
Sbjct: 282 N 284
>TC17553 weakly similar to UP|AAP20176 (AAP20176) Kinase substrate HASPP28
(Fragment), partial (61%)
Length = 754
Score = 27.7 bits (60), Expect = 2.4
Identities = 26/139 (18%), Positives = 56/139 (39%), Gaps = 7/139 (5%)
Frame = +3
Query: 4 ISDYQDDNQTKTSSSSSSQPKPSKPIPFSSTFDPSNPTTFLEKVFDFIAKESTNFFDKDS 63
+ D ++ ++ + +S S+ + +K + NP + + +S D D
Sbjct: 195 VEDEKEVSEEEATSGDESEEETTKTKGTQGVIEIENP--------NLVKPKSLKARDVDL 350
Query: 64 AEKVVLSAVRAARVKKAKSVAAEKAKIAAKEKAKAAAEKK-------LNDEKSEAVTEKI 116
+ LS ++K + A + + +E+ K KK + +++EA ++
Sbjct: 351 EKTTELSRREREEIEKQR---AHERYMRLQEQGKTEQSKKDLERLALIRQQRAEAAKKRD 521
Query: 117 DEKAAPNQGNGMDLEKYSW 135
+EKAA Q + SW
Sbjct: 522 EEKAAKEQKKSEARK*ISW 578
>TC9170 similar to UP|Q9FF36 (Q9FF36) Gb|AAF31027.1, partial (37%)
Length = 457
Score = 27.3 bits (59), Expect = 3.1
Identities = 25/90 (27%), Positives = 40/90 (43%), Gaps = 4/90 (4%)
Frame = +3
Query: 14 KTSSSSSSQPKPSKPIPFSSTFDPSNPTTFL----EKVFDFIAKESTNFFDKDSAEKVVL 69
+T S SS P P +P S +F P++ ++FL K I+ ST SA
Sbjct: 9 QTHYSHSSPPPPPPCLPISPSFSPAS-SSFLAAPFAKTSSSISSASTTLI-SSSATAPSP 182
Query: 70 SAVRAARVKKAKSVAAEKAKIAAKEKAKAA 99
S+ A+ S A+ ++ + KAK +
Sbjct: 183SSSPASPASTCSSAASSQSTAFSSAKAKTS 272
>AV778023
Length = 583
Score = 27.3 bits (59), Expect = 3.1
Identities = 14/30 (46%), Positives = 17/30 (56%)
Frame = -1
Query: 13 TKTSSSSSSQPKPSKPIPFSSTFDPSNPTT 42
+KTS +PK + I FSS PSNP T
Sbjct: 247 SKTSHGLLHRPKRRRRINFSSQTHPSNPRT 158
>TC14311 homologue to UP|Q9FFC0 (Q9FFC0) Histone H2B like protein, partial
(97%)
Length = 626
Score = 27.3 bits (59), Expect = 3.1
Identities = 18/49 (36%), Positives = 27/49 (54%), Gaps = 5/49 (10%)
Frame = +1
Query: 72 VRAARVKKAKSVAAEKAKIAAKEKAKAA-----AEKKLNDEKSEAVTEK 115
+ +++ +A AEK AA+EKA AA AEKK+ E + A +K
Sbjct: 28 INLSKMARADKKPAEKKPAAAEEKAAAAEKKPRAEKKIPKEGAAAGDKK 174
>TC19623 similar to PIR|T52371|T52371 homeobox protein HAT9 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(21%)
Length = 554
Score = 27.3 bits (59), Expect = 3.1
Identities = 24/92 (26%), Positives = 44/92 (47%)
Frame = +1
Query: 15 TSSSSSSQPKPSKPIPFSSTFDPSNPTTFLEKVFDFIAKESTNFFDKDSAEKVVLSAVRA 74
T+++++S PKP+ P+SS +PS T L ++ + S V+S+
Sbjct: 88 TTTTTTSSPKPTVMKPYSSK-EPS-LTLGLSGNNKVYCEDPLELSRQTSPHSDVVSSFST 261
Query: 75 ARVKKAKSVAAEKAKIAAKEKAKAAAEKKLND 106
ARV K + V +I A+E+ + + +D
Sbjct: 262 ARVVKRERVDLSCEEIEAEERLSSRVSDEDDD 357
>TC18866 similar to UP|Q94A79 (Q94A79) AT3g49250/F2K15_110, partial (5%)
Length = 574
Score = 27.3 bits (59), Expect = 3.1
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = -2
Query: 20 SSQPKPSKPIPFSSTFDPSNPT 41
+ PKPS+ IPFS PSN T
Sbjct: 189 NGSPKPSE*IPFSLIIPPSNET 124
>BP072343
Length = 517
Score = 26.9 bits (58), Expect = 4.1
Identities = 18/36 (50%), Positives = 20/36 (55%), Gaps = 2/36 (5%)
Frame = +2
Query: 85 AEKAKIAAKEKAKAAAEKKLNDEKSEA--VTEKIDE 118
AEKA A KA+ AEKKL K +A V E DE
Sbjct: 125 AEKAAAKAALKAQKEAEKKLKKAKKKAGIVEEPADE 232
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.309 0.126 0.350
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,274,855
Number of Sequences: 28460
Number of extensions: 58347
Number of successful extensions: 594
Number of sequences better than 10.0: 69
Number of HSP's better than 10.0 without gapping: 536
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 574
length of query: 289
length of database: 4,897,600
effective HSP length: 89
effective length of query: 200
effective length of database: 2,364,660
effective search space: 472932000
effective search space used: 472932000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 55 (25.8 bits)
Medicago: description of AC134822.6