BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC134822.5 - phase: 0
(195 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8302 47 2e-06
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 37 0.002
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 36 0.004
AV420911 35 0.009
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 35 0.009
TC9247 similar to UP|Q8L6A5 (Q8L6A5) Replication factor C large ... 30 0.21
AV775197 30 0.28
TC10196 homologue to UP|Q39804 (Q39804) BiP isoform B, partial (... 29 0.47
TC12773 28 0.80
BP070833 28 1.0
AV421607 27 1.8
TC9034 27 2.3
TC13616 homologue to UP|Q93YZ7 (Q93YZ7) At2g31810/F20M17.15, par... 27 2.3
BP063554 27 3.1
AV780471 23 3.5
BP068210 26 4.0
TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, par... 26 5.2
BI420016 26 5.2
BP039163 26 5.2
>TC8302
Length = 494
Score = 47.0 bits (110), Expect = 2e-06
Identities = 35/138 (25%), Positives = 65/138 (46%), Gaps = 4/138 (2%)
Frame = +3
Query: 20 QQLYSFKMLESKSISEQLAEFNKILDDLANIEVNMEDEDKALLLLCSLPKSFE----HFK 75
++LY+ +M ES S+S+ + N + L+ + + ++A LLL SLP S++ + K
Sbjct: 3 KRLYTLRMSESTSMSDHIDHMNTLFAQLSASNFTIGENERAELLLESLPDSYDQLVINIK 182
Query: 76 DTILYGKEGTTTLEEIQSALRTKKLTKSKDLRANENSEGLCVSRGNGGGRGNRGSSKSGN 135
+ + L E+ +++ K +++ E L ++RG SKS
Sbjct: 183 NNNIVNHLPLMMLSEVLLEEDSQR*NKEDR*DSSKPMEALTMTRGR---------SKSRK 335
Query: 136 KERYKCFKCHKFGHFKRD 153
K KC+ C + H K+D
Sbjct: 336 KINLKCYHCGQR*HLKKD 389
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 37.0 bits (84), Expect = 0.002
Identities = 22/67 (32%), Positives = 31/67 (45%), Gaps = 3/67 (4%)
Frame = +1
Query: 90 EIQSALRTKKLTKSKDLRANENSEGLCVSRGNGGGRGNRGSSKSGNK---ERYKCFKCHK 146
+ Q A+R + + NS G G GGG G RG +SG KC++C +
Sbjct: 232 DAQDAIRELDGKNGWRVELSHNSRG-----GGGGGGGGRGGGRSGGGGGGSDMKCYECGE 396
Query: 147 FGHFKRD 153
GHF R+
Sbjct: 397 PGHFARE 417
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 36.2 bits (82), Expect = 0.004
Identities = 18/42 (42%), Positives = 22/42 (51%)
Frame = +2
Query: 118 SRGNGGGRGNRGSSKSGNKERYKCFKCHKFGHFKRDFSEDNE 159
SRG GG RG+ G +CFKC K GHF R+ + E
Sbjct: 377 SRGYGGSRGSNGG---------ECFKCGKPGHFARECPSEGE 475
>AV420911
Length = 418
Score = 35.0 bits (79), Expect = 0.009
Identities = 17/36 (47%), Positives = 23/36 (63%)
Frame = +2
Query: 118 SRGNGGGRGNRGSSKSGNKERYKCFKCHKFGHFKRD 153
S+G GGGRG G + G E KC++C + GHF R+
Sbjct: 314 SKGGGGGRGG-GRGRGG--EDLKCYECGEPGHFARE 412
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 35.0 bits (79), Expect = 0.009
Identities = 17/36 (47%), Positives = 23/36 (63%)
Frame = +1
Query: 118 SRGNGGGRGNRGSSKSGNKERYKCFKCHKFGHFKRD 153
S+G GGGRG G + G E KC++C + GHF R+
Sbjct: 106 SKGGGGGRGG-GRGRGG--EDLKCYECGEPGHFARE 204
>TC9247 similar to UP|Q8L6A5 (Q8L6A5) Replication factor C large subunit
(Fragment), partial (10%)
Length = 884
Score = 30.4 bits (67), Expect = 0.21
Identities = 19/52 (36%), Positives = 27/52 (51%), Gaps = 3/52 (5%)
Frame = +1
Query: 84 GTTTLEEIQS---ALRTKKLTKSKDLRANENSEGLCVSRGNGGGRGNRGSSK 132
GTTT E++QS +L +K +L+ E S S G G GRG S++
Sbjct: 229 GTTTGEKLQSELQSLNSKATQVQLELKGTEKSSSNKTSGGRGRGRGASASAQ 384
>AV775197
Length = 439
Score = 30.0 bits (66), Expect = 0.28
Identities = 17/49 (34%), Positives = 23/49 (46%)
Frame = +3
Query: 91 IQSALRTKKLTKSKDLRANENSEGLCVSRGNGGGRGNRGSSKSGNKERY 139
IQ+ TKK K + +R E + G GGG SS+SG + Y
Sbjct: 183 IQNKTATKKKDKERKIRKQERNLITYTQSGKGGGE*VSESSESGTRPSY 329
>TC10196 homologue to UP|Q39804 (Q39804) BiP isoform B, partial (17%)
Length = 562
Score = 29.3 bits (64), Expect = 0.47
Identities = 28/109 (25%), Positives = 50/109 (45%)
Frame = +2
Query: 29 ESKSISEQLAEFNKILDDLANIEVNMEDEDKALLLLCSLPKSFEHFKDTILYGKEGTTTL 88
E K + E++ + + + N++ + ++DK L S K E + + KE L
Sbjct: 17 EDKKVKERIDARHGLETYIYNMKNQVSEKDKLADKLESDEK--EKIETAV---KEALEWL 181
Query: 89 EEIQSALRTKKLTKSKDLRANENSEGLCVSRGNGGGRGNRGSSKSGNKE 137
++ QSA + + K K++ A N V + +GG G G+S G E
Sbjct: 182 DDNQSAEKEEYEEKLKEVEAVCNPIITAVYQRSGGAPGGEGASGEGEDE 328
>TC12773
Length = 420
Score = 28.5 bits (62), Expect = 0.80
Identities = 17/58 (29%), Positives = 26/58 (44%), Gaps = 7/58 (12%)
Frame = +2
Query: 103 SKDLRANENSEGLC-------VSRGNGGGRGNRGSSKSGNKERYKCFKCHKFGHFKRD 153
S+D N +S G+ S G +R S+K + Y+C+KC + GH D
Sbjct: 212 SQDQVKNFSSSGISDALPLPVFSDGTWDHNRHRWSNKRSSLSTYECWKCQRPGHMAED 385
>BP070833
Length = 433
Score = 28.1 bits (61), Expect = 1.0
Identities = 17/69 (24%), Positives = 29/69 (41%), Gaps = 2/69 (2%)
Frame = +2
Query: 111 NSEGLCVSRGNGGGRGNRGSSKSGNKERYKCFKCHKFGHFKRDFSEDNENF--AQVVSEE 168
N + LC G R SS SG + Y C + F K + E+F A+++ +
Sbjct: 188 NMDSLCCFNSANQAGGGRSSSSSGKGKSYLCPVKYGFSLVKGRANHPMEDFHVAKIIQNK 367
Query: 169 YEDAGALVV 177
++ G +
Sbjct: 368 GQELGLFAI 394
>AV421607
Length = 245
Score = 27.3 bits (59), Expect = 1.8
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +3
Query: 120 GNGGGRGNRGSSKSGNKERYKCFKCHKFGHFKRD 153
G+G G G+ + +G C+KC + GH+ RD
Sbjct: 9 GSGPGSGSGSGTATG------CYKCGRPGHWSRD 92
>TC9034
Length = 583
Score = 26.9 bits (58), Expect = 2.3
Identities = 13/50 (26%), Positives = 21/50 (42%)
Frame = +1
Query: 117 VSRGNGGGRGNRGSSKSGNKERYKCFKCHKFGHFKRDFSEDNENFAQVVS 166
++R G G S ++ R KC C +FGH + D + V+
Sbjct: 76 INRKRGVTEGPTVSHEARRSHRVKCANCEEFGHDSWECQRDKSKRQEKVT 225
>TC13616 homologue to UP|Q93YZ7 (Q93YZ7) At2g31810/F20M17.15, partial (9%)
Length = 440
Score = 26.9 bits (58), Expect = 2.3
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = -3
Query: 116 CVSRGNGGGRGNRGSSKSGNKERY 139
CVS G GGG G ++SG + RY
Sbjct: 270 CVSGGCGGGEGEFVVAESGGRVRY 199
>BP063554
Length = 526
Score = 26.6 bits (57), Expect = 3.1
Identities = 16/44 (36%), Positives = 20/44 (45%), Gaps = 5/44 (11%)
Frame = +1
Query: 155 SEDNENFAQVVSEE-----YEDAGALVVSCWEDDEGEVSHLDID 193
S DN NF SE +D L+ W+ E SH+DID
Sbjct: 187 SLDNWNFLHFFSENGFLIIEDDGSMLIEKFWQGCECPSSHVDID 318
>AV780471
Length = 527
Score = 23.1 bits (48), Expect(2) = 3.5
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = -2
Query: 110 ENSEGLCVSRGNGGGRGNRGSSKSGNKERYKCFK 143
+N+ +C G GGG G G KSG ++ +K
Sbjct: 247 QNN*SVC---GGGGGGGGGGRRKSGGGGFFRWWK 155
Score = 21.6 bits (44), Expect(2) = 3.5
Identities = 7/17 (41%), Positives = 11/17 (64%)
Frame = -3
Query: 177 VSCWEDDEGEVSHLDID 193
+ WE DEG V L+++
Sbjct: 99 IRVWEGDEGRVHDLNLE 49
>BP068210
Length = 412
Score = 26.2 bits (56), Expect = 4.0
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +1
Query: 46 DLANIEVNMEDEDKALLLLCSLPKS 70
+ +N E+N E +D A L L SLP S
Sbjct: 337 ECSNSEINAEAQDSAQLTLSSLPTS 411
>TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (62%)
Length = 684
Score = 25.8 bits (55), Expect = 5.2
Identities = 15/40 (37%), Positives = 18/40 (44%), Gaps = 1/40 (2%)
Frame = +2
Query: 115 LCVSRGNGGGRGNRGSSKSGNKERY-KCFKCHKFGHFKRD 153
+ V GG RGNR G +CF C GH+ RD
Sbjct: 290 IVVEFAKGGPRGNREYLGRGPPPGSGRCFNCGLDGHWARD 409
>BI420016
Length = 559
Score = 25.8 bits (55), Expect = 5.2
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = -1
Query: 107 RANENSEGLCVSRGNGGGRGNRGSSKSGNK 136
RA + G C G GGGRG RG + G +
Sbjct: 532 RAKADHGGCCRRGGGGGGRG-RGRREEGGR 446
>BP039163
Length = 544
Score = 25.8 bits (55), Expect = 5.2
Identities = 10/23 (43%), Positives = 13/23 (56%)
Frame = +2
Query: 120 GNGGGRGNRGSSKSGNKERYKCF 142
G GGG G R S + R++CF
Sbjct: 410 GEGGGGGGRRSHGGRSSNRWRCF 478
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.313 0.132 0.368
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,872,821
Number of Sequences: 28460
Number of extensions: 32647
Number of successful extensions: 206
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 200
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 202
length of query: 195
length of database: 4,897,600
effective HSP length: 85
effective length of query: 110
effective length of database: 2,478,500
effective search space: 272635000
effective search space used: 272635000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 53 (25.0 bits)
Medicago: description of AC134822.5