BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC134822.4 - phase: 0
(290 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14246 similar to UP|Q84LL6 (Q84LL6) Salt tolerance protein 5, ... 378 e-106
TC8259 similar to UP|HMGL_SOYBN (P26585) HMG1/2-like protein (SB... 36 0.009
TC19623 similar to PIR|T52371|T52371 homeobox protein HAT9 [impo... 31 0.28
TC16625 similar to UP|RS12_BUCBP (Q89A65) 30S ribosomal protein ... 30 0.37
TC8366 weakly similar to UP|O24088 (O24088) MtN24 protein, parti... 28 1.4
TC18866 similar to UP|Q94A79 (Q94A79) AT3g49250/F2K15_110, parti... 28 1.8
BP057759 28 1.8
BG662097 28 2.4
TC14264 similar to UP|Q84NF9 (Q84NF9) Histone H1 (Fragment), par... 28 2.4
AV420631 28 2.4
BP067321 27 3.1
CB829423 27 3.1
TC9170 similar to UP|Q9FF36 (Q9FF36) Gb|AAF31027.1, partial (37%) 27 4.1
TC15487 similar to UP|O23144 (O23144) Proton pump interactor (AT... 27 4.1
BP067133 27 4.1
TC18665 similar to UP|O23061 (O23061) BAC IG005I10, partial (65%) 27 4.1
BI419469 27 5.3
TC12903 weakly similar to UP|ALA9_ARATH (Q9SX33) Potential phosp... 27 5.3
TC16736 similar to UP|BAD09309 (BAD09309) Type 1a plasma membran... 27 5.3
AV410933 27 5.3
>TC14246 similar to UP|Q84LL6 (Q84LL6) Salt tolerance protein 5, partial
(57%)
Length = 1280
Score = 378 bits (970), Expect = e-106
Identities = 195/301 (64%), Positives = 239/301 (78%), Gaps = 11/301 (3%)
Frame = +1
Query: 1 MAIISDYQDE---------TQTPSSSSQPKPSKTIPFSSTFDPSKPTAFLEKVFDFIAKE 51
MAIISD+ +E ++T +S SQ KPS + F++TFD S P AFLE+VFDF+++E
Sbjct: 97 MAIISDFHEEEPQQNNAAPSKTDASPSQSKPSPSPTFAATFDRSNPAAFLERVFDFVSRE 276
Query: 52 STDFFDKDSAEKMVLSAVRAAKVKKAKAVAAEKAKIASQEKAKAAAGIKVNDEKSGVGTE 111
S DF ++AEK V S VRAA+ K+ K E+ AS+EKAKA +K ++K E
Sbjct: 277 S-DFLATEAAEKEVASLVRAAREKRKKK--REEELKASEEKAKAEKRLKEAEKKPKA--E 441
Query: 112 KKDGESGLAA--PNQGNGMDLEKYSWTQTLQELNVNVPVPNGTKSGFVICEIKKNHLKVG 169
+ D + G AA PN+GNGMDLEKYSWTQTLQE+ VNVPVP+GTKS FV+CEIKKNHLKVG
Sbjct: 442 ENDKKEGTAAIVPNKGNGMDLEKYSWTQTLQEVTVNVPVPHGTKSRFVVCEIKKNHLKVG 621
Query: 170 LKGQPPIIDGELYKFIKADECYWSIEDQSTVSILLTKHDQMDWWKCLVKGDPLINTQKVE 229
LKGQPPII+GE ++ +K D+CYWSIEDQS +SILLTKHDQMDWWKCLVKGDP I+TQKVE
Sbjct: 622 LKGQPPIIEGEFFRPVKPDDCYWSIEDQSALSILLTKHDQMDWWKCLVKGDPEIDTQKVE 801
Query: 230 PESSKLGELDSETRMTVEKMMFDQRQKSMGLPTSEELEKQEMMKKFMSQHPNMDFSGAKL 289
PE+S+L +LD+ETR TVEKMMFDQRQKSMGLPTS+EL+KQE++KKFMS+HP MDFS AKL
Sbjct: 802 PENSRLSDLDAETRQTVEKMMFDQRQKSMGLPTSDELQKQELLKKFMSEHPEMDFSRAKL 981
Query: 290 S 290
+
Sbjct: 982 A 984
>TC8259 similar to UP|HMGL_SOYBN (P26585) HMG1/2-like protein (SB11
protein), partial (92%)
Length = 942
Score = 35.8 bits (81), Expect = 0.009
Identities = 33/113 (29%), Positives = 54/113 (47%), Gaps = 4/113 (3%)
Frame = +2
Query: 21 KPSKTIPFSSTFDPSKPTAFLEKVFDFIAKESTDFFDKDSAEKMVLSAVRAAKVKKAKAV 80
KP++ + DP+KP F F+ ++ F+K++ E +SAV A K K++
Sbjct: 257 KPARGAKGKAAKDPNKPKRPPSAFFVFM-EDFRKIFNKENPENKAVSAVGKAAGAKWKSM 433
Query: 81 A-AEKAK-IASQEKAKA--AAGIKVNDEKSGVGTEKKDGESGLAAPNQGNGMD 129
+ AEKA +A EK KA +K ++K G D E + ++ N D
Sbjct: 434 SEAEKAPYVAKAEKRKADYEKTMKAYNKKQAEGPAAADEEESEKSVSEVNDQD 592
>TC19623 similar to PIR|T52371|T52371 homeobox protein HAT9 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(21%)
Length = 554
Score = 30.8 bits (68), Expect = 0.28
Identities = 29/105 (27%), Positives = 50/105 (47%), Gaps = 1/105 (0%)
Frame = +1
Query: 10 ETQTPSSSSQPKPSKTIPFSSTFDPSKPTAFLEKVFDFIAKESTDFFDKDSAEKMVLSAV 69
ET T +++S PKP+ P+SS +PS T L ++ + + S V+S+
Sbjct: 82 ETTTTTTTSSPKPTVMKPYSSK-EPSL-TLGLSGNNKVYCEDPLELSRQTSPHSDVVSSF 255
Query: 70 RAAKVKKAKAVAAEKAKIASQEKAKAAAGIKVNDE-KSGVGTEKK 113
A+V K + V +I ++E+ + +V+DE G KK
Sbjct: 256 STARVVKRERVDLSCEEIEAEERLSS----RVSDEDDDGTNARKK 378
>TC16625 similar to UP|RS12_BUCBP (Q89A65) 30S ribosomal protein S12,
partial (13%)
Length = 814
Score = 30.4 bits (67), Expect = 0.37
Identities = 13/32 (40%), Positives = 21/32 (65%)
Frame = -2
Query: 191 YWSIEDQSTVSILLTKHDQMDWWKCLVKGDPL 222
Y+++EDQS + L TK + W+ CL+ DP+
Sbjct: 444 YYTLEDQSNMK-LTTKQRKKKWYICLLNPDPV 352
>TC8366 weakly similar to UP|O24088 (O24088) MtN24 protein, partial (28%)
Length = 612
Score = 28.5 bits (62), Expect = 1.4
Identities = 24/84 (28%), Positives = 33/84 (38%)
Frame = +1
Query: 78 KAVAAEKAKIASQEKAKAAAGIKVNDEKSGVGTEKKDGESGLAAPNQGNGMDLEKYSWTQ 137
K + EKAK A++E K D GTE DG G G M ++ W +
Sbjct: 106 KEESEEKAKDAAEETKVEEGLKKKEDTDPETGTEGGDGGGGDDGSVSGFLMSKKEEKWCR 285
Query: 138 TLQELNVNVPVPNGTKSGFVICEI 161
L + + S FV+C I
Sbjct: 286 ALLGVRI---------SAFVLCLI 330
>TC18866 similar to UP|Q94A79 (Q94A79) AT3g49250/F2K15_110, partial (5%)
Length = 574
Score = 28.1 bits (61), Expect = 1.8
Identities = 14/29 (48%), Positives = 16/29 (54%)
Frame = -2
Query: 11 TQTPSSSSQPKPSKTIPFSSTFDPSKPTA 39
TQ + PKPS+ IPFS PS TA
Sbjct: 207 TQGKHINGSPKPSE*IPFSLIIPPSNETA 121
>BP057759
Length = 563
Score = 28.1 bits (61), Expect = 1.8
Identities = 15/38 (39%), Positives = 20/38 (52%)
Frame = +3
Query: 16 SSSQPKPSKTIPFSSTFDPSKPTAFLEKVFDFIAKEST 53
SSS S + P S + SKPT F K+F +I + T
Sbjct: 222 SSSPNSRSVSRPRSPAINASKPTIFASKMFSWITTKGT 335
>BG662097
Length = 350
Score = 27.7 bits (60), Expect = 2.4
Identities = 20/67 (29%), Positives = 35/67 (51%), Gaps = 8/67 (11%)
Frame = -2
Query: 53 TDFFDKDSAEKMVLSAVRAAKVKK--------AKAVAAEKAKIASQEKAKAAAGIKVNDE 104
T K AE M+ AV K ++ +K ++A++A IASQEK+ A+ +++D
Sbjct: 346 TKKIQKKLAEAMIAKAVADEKERELENQVEGLSKDLSAKEATIASQEKSLASKVKEISDL 167
Query: 105 KSGVGTE 111
+ T+
Sbjct: 166 HERLATK 146
>TC14264 similar to UP|Q84NF9 (Q84NF9) Histone H1 (Fragment), partial (76%)
Length = 1277
Score = 27.7 bits (60), Expect = 2.4
Identities = 32/97 (32%), Positives = 41/97 (41%), Gaps = 8/97 (8%)
Frame = +2
Query: 12 QTPSSSSQPKPSKTIPFSSTFDPSKPTAFLEKVFDFIAKESTDFFDKDSAEKMVLSAVR- 70
Q ++ S+PKP +KP A K A +S K +A+ +A +
Sbjct: 503 QPAAAKSKPKPKAK---------AKPAAAATKAKAKPAPKSKAAATKTTAKAKPAAAAKP 655
Query: 71 ----AAKVK---KAKAVAAEKAKIASQEKAKAAAGIK 100
AAK K KAKAVAA AS K KA A K
Sbjct: 656 KAKPAAKAKPAAKAKAVAAPAKAKASPAKPKAKAKSK 766
Score = 26.9 bits (58), Expect = 4.1
Identities = 24/89 (26%), Positives = 34/89 (37%), Gaps = 12/89 (13%)
Frame = +2
Query: 73 KVKKAKAVAAEKAKIASQEKAKAAAGIKVNDEKSGVGTEKKDGESGLAAP---------- 122
K K A AA KAK K+KAAA K + K + A P
Sbjct: 536 KAKAKPAAAATKAKAKPAPKSKAAATKTTAKAKPAAAAKPKAKPAAKAKPAAKAKAVAAP 715
Query: 123 --NQGNGMDLEKYSWTQTLQELNVNVPVP 149
+ + + + ++T +NVN PVP
Sbjct: 716 AKAKASPAKPKAKAKSKTAPRMNVNPPVP 802
>AV420631
Length = 355
Score = 27.7 bits (60), Expect = 2.4
Identities = 19/62 (30%), Positives = 27/62 (42%)
Frame = +1
Query: 33 DPSKPTAFLEKVFDFIAKESTDFFDKDSAEKMVLSAVRAAKVKKAKAVAAEKAKIASQEK 92
DP P ++++F I D K + AK ++ VAAEKA+ AS K
Sbjct: 184 DPEAPLKRVDELFSTIPV--------DGRRKAYAETLEKAKAEEEAIVAAEKAREASATK 339
Query: 93 AK 94
K
Sbjct: 340 KK 345
>BP067321
Length = 513
Score = 27.3 bits (59), Expect = 3.1
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = -1
Query: 11 TQTPSSSSQPKPSKTIPFSST 31
T+ SSSS P S T+PF ST
Sbjct: 480 TEPSSSSSSPSRSSTVPFRST 418
>CB829423
Length = 383
Score = 27.3 bits (59), Expect = 3.1
Identities = 12/25 (48%), Positives = 13/25 (52%)
Frame = -3
Query: 16 SSSQPKPSKTIPFSSTFDPSKPTAF 40
S QP P +PF S F PS T F
Sbjct: 219 SQHQPHPLFVVPFQSRFQPSNITQF 145
>TC9170 similar to UP|Q9FF36 (Q9FF36) Gb|AAF31027.1, partial (37%)
Length = 457
Score = 26.9 bits (58), Expect = 4.1
Identities = 23/85 (27%), Positives = 35/85 (41%), Gaps = 3/85 (3%)
Frame = +3
Query: 15 SSSSQPKPSKTIPFSSTFDPSKPT---AFLEKVFDFIAKESTDFFDKDSAEKMVLSAVRA 71
S SS P P +P S +F P+ + A K I+ ST SA S+ A
Sbjct: 21 SHSSPPPPPPCLPISPSFSPASSSFLAAPFAKTSSSISSASTTLI-SSSATAPSPSSSPA 197
Query: 72 AKVKKAKAVAAEKAKIASQEKAKAA 96
+ + A+ ++ S KAK +
Sbjct: 198 SPASTCSSAASSQSTAFSSAKAKTS 272
>TC15487 similar to UP|O23144 (O23144) Proton pump interactor
(AT4g27500/F27G19_100), partial (16%)
Length = 1153
Score = 26.9 bits (58), Expect = 4.1
Identities = 25/110 (22%), Positives = 41/110 (36%), Gaps = 10/110 (9%)
Frame = +2
Query: 14 PSSSSQPKPSKTIPFSSTFD---------PSKPTAFLEKVFDF-IAKESTDFFDKDSAEK 63
P +P P +T+P P K ++F + ++ T + +
Sbjct: 257 PKEEPKPSPQETLPTQKESKNKVRDLKAKPDKKDLEEADEYEFEVPRKETPPKEPEIDPA 436
Query: 64 MVLSAVRAAKVKKAKAVAAEKAKIASQEKAKAAAGIKVNDEKSGVGTEKK 113
+ R ++ KAK K K+A + AKAA + EK EKK
Sbjct: 437 KLKEMKREEEIAKAKQAWERKKKLAEKAAAKAALKAQKEAEKKLKDREKK 586
>BP067133
Length = 507
Score = 26.9 bits (58), Expect = 4.1
Identities = 14/27 (51%), Positives = 18/27 (65%), Gaps = 4/27 (14%)
Frame = +1
Query: 13 TPSSSSQPKPSKT----IPFSSTFDPS 35
TP+SS PS+T IPFS+T+ PS
Sbjct: 361 TPASSPPSAPSRTSPPSIPFSNTWTPS 441
>TC18665 similar to UP|O23061 (O23061) BAC IG005I10, partial (65%)
Length = 451
Score = 26.9 bits (58), Expect = 4.1
Identities = 12/28 (42%), Positives = 17/28 (59%)
Frame = +2
Query: 9 DETQTPSSSSQPKPSKTIPFSSTFDPSK 36
+ + SSSS P PS + P SS+ PS+
Sbjct: 95 EASSVASSSSSPSPSSSSPISSSRKPSE 178
>BI419469
Length = 432
Score = 26.6 bits (57), Expect = 5.3
Identities = 14/42 (33%), Positives = 24/42 (56%)
Frame = -3
Query: 227 KVEPESSKLGELDSETRMTVEKMMFDQRQKSMGLPTSEELEK 268
+V+PE + E D+ETR V +++ + R +P EEL +
Sbjct: 160 EVDPEGN---ESDAETRCGVTQLIAEHRMLPPAVPPPEELRR 44
>TC12903 weakly similar to UP|ALA9_ARATH (Q9SX33) Potential
phospholipid-transporting ATPase 9 (Aminophospholipid
flippase 9) , partial (14%)
Length = 746
Score = 26.6 bits (57), Expect = 5.3
Identities = 14/29 (48%), Positives = 16/29 (54%), Gaps = 6/29 (20%)
Frame = -3
Query: 12 QTPSSSSQPKPSK------TIPFSSTFDP 34
+ PS+SSQPKP K T PF F P
Sbjct: 258 RVPSASSQPKPRKCTWLPPTCPFRRFFPP 172
>TC16736 similar to UP|BAD09309 (BAD09309) Type 1a plasma membrane
receptor-like, partial (22%)
Length = 383
Score = 26.6 bits (57), Expect = 5.3
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +2
Query: 129 DLEKYSWTQTLQELNVNVPVPNGT 152
+LE W+ LQ LNVN P P T
Sbjct: 191 ELEMQVWSFLLQPLNVNCPYPFNT 262
>AV410933
Length = 422
Score = 26.6 bits (57), Expect = 5.3
Identities = 11/17 (64%), Positives = 13/17 (75%)
Frame = +3
Query: 220 DPLINTQKVEPESSKLG 236
DPLI QK+ PE+SK G
Sbjct: 297 DPLIYLQKIAPEASKYG 347
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.310 0.127 0.355
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,133,528
Number of Sequences: 28460
Number of extensions: 51953
Number of successful extensions: 351
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 344
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 348
length of query: 290
length of database: 4,897,600
effective HSP length: 89
effective length of query: 201
effective length of database: 2,364,660
effective search space: 475296660
effective search space used: 475296660
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 55 (25.8 bits)
Medicago: description of AC134822.4