BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC134322.1 + phase: 0 /pseudo
(413 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CN825238 69 1e-12
AV411435 60 5e-10
BE122611 48 2e-09
AV428268 51 3e-07
AV418280 47 8e-06
CB826728 40 8e-06
BP076564 38 0.003
TC11350 similar to UP|Q9LJP7 (Q9LJP7) Genomic DNA, chromosome 3,... 38 0.003
AV412895 38 0.003
BI418523 37 0.008
AV780577 36 0.013
TC19933 35 0.023
BP080173 34 0.039
TC9793 34 0.051
AV426428 33 0.086
CB828953 32 0.15
TC11323 32 0.15
AV780445 32 0.15
AV414192 31 0.43
BP056414 30 0.56
>CN825238
Length = 692
Score = 68.9 bits (167), Expect = 1e-12
Identities = 39/132 (29%), Positives = 64/132 (47%), Gaps = 3/132 (2%)
Frame = +3
Query: 56 LLSQHGKSWNEPLVQQMFSNDIAEAILHTPLYDQVTNDCLVWKAERNGCYSVRSAYR--L 113
LL + + WN L+ Q+F + A I PL + T + +W+ RNG +SVRSAY
Sbjct: 297 LLIPNARRWNVQLIDQLFPKETAAVIKSIPLGWRTTRNSCLWRWSRNGAFSVRSAYYNIQ 476
Query: 114 YVEELIDVSHLRRPGNWQHIWYLKVSPKVKNLLWCMCRGCLPT*VRLQDKGVSCPTNCES 173
+E+ S W+ +W V K+K+ + + R +P LQ +G++ C +
Sbjct: 477 VAKEMQTSSSAASSFPWKKLWRTPVPNKMKHFAYRVARNIIPCRENLQRRGIAVQNECPT 656
Query: 174 CGAD-HEDLNHL 184
C E ++HL
Sbjct: 657 CNHQIPESMSHL 692
>AV411435
Length = 426
Score = 60.5 bits (145), Expect = 5e-10
Identities = 35/121 (28%), Positives = 53/121 (42%), Gaps = 6/121 (4%)
Frame = +3
Query: 81 ILHTPLYDQVTNDCLVWKAERNGCYSVRSAYRLYVEELIDVSHLRRPGN------WQHIW 134
I+ TP + D L W +G YSV+S YR ++S+L + W IW
Sbjct: 3 IMQTPFSITGSEDRLFWPFIPSGEYSVKSGYRAVKATQFNLSNLASSSSHPSSFLWHTIW 182
Query: 135 YLKVSPKVKNLLWCMCRGCLPT*VRLQDKGVSCPTNCESCGADHEDLNHLLFECPFSIHV 194
+V KV++ LW +P L+ + + C C ED+NH L C ++ V
Sbjct: 183 GAQVPKKVRSFLWRAANNAIPVKRNLKRRNMGRDDFCPICNKGPEDINHALLACEWTRAV 362
Query: 195 W 195
W
Sbjct: 363 W 365
>BE122611
Length = 357
Score = 48.1 bits (113), Expect(2) = 2e-09
Identities = 24/74 (32%), Positives = 36/74 (48%)
Frame = -3
Query: 86 LYDQVTNDCLVWKAERNGCYSVRSAYRLYVEELIDVSHLRRPGNWQHIWYLKVSPKVKNL 145
L+ + D ++W E NGCYS +S Y L +E+ + S W +W KV+
Sbjct: 352 LHSRRGTDRILWTEESNGCYSAKSTYNLLIEDGGNESRF-----WSLVWKANAPEKVRFF 188
Query: 146 LWCMCRGCLPT*VR 159
LW + R LP+ R
Sbjct: 187 LWLVGRNALPSNAR 146
Score = 30.4 bits (67), Expect(2) = 2e-09
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = -2
Query: 171 CESCGADHEDLNHLLFECPFSIHVW 195
C CGA ED NH+ CP S +W
Sbjct: 113 CIRCGAPSEDANHVFRWCPGSAWMW 39
>AV428268
Length = 429
Score = 51.2 bits (121), Expect = 3e-07
Identities = 42/150 (28%), Positives = 58/150 (38%), Gaps = 4/150 (2%)
Frame = +3
Query: 105 YSVRSAYRLYVEELIDVSHLRRPGNWQHI----WYLKVSPKVKNLLWCMCRGCLPT*VRL 160
YSV+SAY D H W +I W +KV L W + + T V L
Sbjct: 6 YSVKSAY--------DFLHGCANAQWDNIFKLLWSVKVPSNAIALSWKVLINRVQTKVNL 161
Query: 161 QDKGVSCPTNCESCGADHEDLNHLLFECPFSIHVWNSAGIWHDVQHAAIHSDSAANTIFS 220
+GV C C D E +HLLF CP +W+ V + +DS +
Sbjct: 162 NRRGVVISNVCPLCSLDEESTDHLLFSCPIV*RIWSKISE*FGV-FSVFPNDSHGH---- 326
Query: 221 ILQNLSRNIKQRFAATCWSLWKHRNLKIWE 250
LQ+L F W +W + IW+
Sbjct: 327 FLQHLGSCGSLNFRKRGWFVWIAAVVCIWQ 416
>AV418280
Length = 398
Score = 46.6 bits (109), Expect = 8e-06
Identities = 35/109 (32%), Positives = 47/109 (43%), Gaps = 6/109 (5%)
Frame = -2
Query: 171 CESCGADHEDLNHLLFECPFSIHVWNSAGIWHDVQHAAIHSDSAANTIFSILQNLSRNIK 230
C C E HL F C FS+ VW + W + A S A FSI +RN
Sbjct: 388 CPFCTTVEECSGHLFFTCVFSMGVWQALHRWLGISVALPASTLANFAQFSI---TARNKN 218
Query: 231 QR------FAATCWSLWKHRNLKIWENVDENSVQVVDRARNLIADWEEA 273
QR + AT WSLW RN I+ N + ++D ++ W +A
Sbjct: 217 QRLGELAIWIATVWSLWIQRNSIIFRNNALDHSYLLDLIQSRSWHWLKA 71
>CB826728
Length = 509
Score = 40.4 bits (93), Expect(2) = 8e-06
Identities = 24/98 (24%), Positives = 38/98 (38%), Gaps = 6/98 (6%)
Frame = +1
Query: 97 WKAERNGCYSVRSAYRLYVEELIDVSHLRRPGN------WQHIWYLKVSPKVKNLLWCMC 150
W +G YS +S Y E + P W+ +W P+ K ++W C
Sbjct: 214 WPGSSDGWYSSKSGYEFIRLENKSLLPSTSPAAAVPSLIWKTVWSASSLPRCKEVMWRAC 393
Query: 151 RGCLPT*VRLQDKGVSCPTNCESCGADHEDLNHLLFEC 188
G LP L+ +G+ + G + E H+L C
Sbjct: 394 AGYLPVRSALKRRGLDVDPSSPWSGLEDEAEVHVLLTC 507
Score = 25.4 bits (54), Expect(2) = 8e-06
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = +3
Query: 57 LSQHGKSWNEPLVQQMFSNDIAEAILHTPLYDQVTNDCL 95
L ++G+ WNE LV +FS L L Q DCL
Sbjct: 96 LIENGR-WNENLVSMVFSPSTTAVFLAVSLPLQSYEDCL 209
>BP076564
Length = 371
Score = 38.1 bits (87), Expect = 0.003
Identities = 28/110 (25%), Positives = 44/110 (39%)
Frame = +2
Query: 136 LKVSPKVKNLLWCMCRGCLPT*VRLQDKGVSCPTNCESCGADHEDLNHLLFECPFSIHVW 195
LKV K K ++ LP R ++ C C A +D+ H L +CP S VW
Sbjct: 56 LKVVEKCKFMVGLGLHQALPINARRHACSMAATGGCGRCSAAVKDILHCLQDCPHSREVW 235
Query: 196 NSAGIWHDVQHAAIHSDSAANTIFSILQNLSRNIKQRFAATCWSLWKHRN 245
N G S + + SI++ + ++ W +W+ RN
Sbjct: 236 NRLGC---SSFGGFLSSDGWSWLLSIMKQPN---VEKLIYILWWIWR*RN 367
>TC11350 similar to UP|Q9LJP7 (Q9LJP7) Genomic DNA, chromosome 3, P1 clone:
MIG10, partial (18%)
Length = 1118
Score = 38.1 bits (87), Expect = 0.003
Identities = 37/169 (21%), Positives = 61/169 (35%)
Frame = -3
Query: 236 TCWSLWKHRNLKIWENVDENSVQVVDRARNLIADWEEAITLQPATFLQEDVQQQ*PAVPL 295
T W++WK+RN I++ + N V + + R++ D T Q
Sbjct: 981 TLWAIWKNRNRVIFQLEEPNPVNTIFQIRHMQQDANLMSTSQSNK--------------- 847
Query: 296 QLHAQHQQSAVPLQAHAHHHNRPAGHGNLKWTPPSSGRFKCNVDAAFSAQFQRTGIGVCI 355
Q + + W PP G +K N DA++ A +GV I
Sbjct: 846 -------------QTTRERNINVTSARDRSWRPPPLGVYKLNSDASWKAPSPSCTVGVVI 706
Query: 356 RDDS*TFVLAKVLQFDQLFPVAIGEALGLYHAMQWMQDMYFDNIDFELD 404
RD+ + + P+ + EAL L + + + D I E D
Sbjct: 705 RDNLGLLISGTASRPLAPSPI-VSEALALREGLILARSLGLDRILSESD 562
>AV412895
Length = 405
Score = 37.7 bits (86), Expect = 0.003
Identities = 28/112 (25%), Positives = 45/112 (40%), Gaps = 3/112 (2%)
Frame = +1
Query: 133 IWYLKVSPKVKNLLWCMCRGCLPT*VRLQDKGV---SCPTNCESCGADHEDLNHLLFECP 189
IW + +K +W + G + T L + V + C CG E +HLLF C
Sbjct: 82 IWMVPAPLNIKAFVWRVLLGRIQTRDNLLKRQVIHNALDAICPLCGLAEESGSHLLFSCA 261
Query: 190 FSIHVWNSAGIWHDVQHAAIHSDSAANTIFSILQNLSRNIKQRFAATCWSLW 241
S+ +W W V A + FS ++ R+ Q+ T ++W
Sbjct: 262 ESMLIWYECFAWLGVSTAQVSDPKVHLLQFS---SIGRSKAQKLGET--TIW 402
>BI418523
Length = 496
Score = 36.6 bits (83), Expect = 0.008
Identities = 25/86 (29%), Positives = 39/86 (45%), Gaps = 4/86 (4%)
Frame = +1
Query: 326 WTPPSSGRFKCNVDAAFSAQFQRTGIGVCIRDDS*TFVLAKVLQFDQLFP----VAIGEA 381
W+PPS+G KCNVD A +G+G RD + K+L + L +
Sbjct: 187 WSPPSAGFIKCNVDGASQGNPGPSGVGGVFRDAN-----RKILGYFSLNSGNGWAYEAKV 351
Query: 382 LGLYHAMQWMQDMYFDNIDFELDSKI 407
+ +A+ ++Q NI E DS +
Sbjct: 352 RSILNALVFIQKFLLKNILIESDSTV 429
>AV780577
Length = 486
Score = 35.8 bits (81), Expect = 0.013
Identities = 22/73 (30%), Positives = 33/73 (45%)
Frame = +2
Query: 322 GNLKWTPPSSGRFKCNVDAAFSAQFQRTGIGVCIRDDS*TFVLAKVLQFDQLFPVAIGEA 381
G W PP G+ K N+ Q G G +RD + +++ + + VA+GEA
Sbjct: 224 GPSTWWPPPHGKLKINIVDVVVPQVDMMGFGFVMRDANGLVLVSGASKHRRSLSVAMGEA 403
Query: 382 LGLYHAMQWMQDM 394
L AMQ D+
Sbjct: 404 LA*RWAMQVSFDL 442
>TC19933
Length = 472
Score = 35.0 bits (79), Expect = 0.023
Identities = 16/45 (35%), Positives = 22/45 (48%)
Frame = -3
Query: 130 WQHIWYLKVSPKVKNLLWCMCRGCLPT*VRLQDKGVSCPTNCESC 174
W I KVS KVK+ LW +C +P L+ +G + C C
Sbjct: 143 WSSI*KAKVSNKVKHFLWKLCHNAIPLKGNLRRRGCATDGKCPIC 9
>BP080173
Length = 382
Score = 34.3 bits (77), Expect = 0.039
Identities = 23/89 (25%), Positives = 37/89 (40%)
Frame = -2
Query: 317 RPAGHGNLKWTPPSSGRFKCNVDAAFSAQFQRTGIGVCIRDDS*TFVLAKVLQFDQLFPV 376
R +G ++W P G K N+D +F+ G + D TF+ ++ F +
Sbjct: 372 RASGTTFVRWPVPMEGWIKINIDGSFAPSSNCAACGGVMWDHEGTFITGASVRLGCYF-I 196
Query: 377 AIGEALGLYHAMQWMQDMYFDNIDFELDS 405
E G++H + Q F I E DS
Sbjct: 195 NHAELWGIFHGAKIAQARGFKKILVESDS 109
>TC9793
Length = 568
Score = 33.9 bits (76), Expect = 0.051
Identities = 24/89 (26%), Positives = 41/89 (45%), Gaps = 1/89 (1%)
Frame = +2
Query: 326 WTPPSSGRFKCNVDAAFSAQFQRTGIGVCIRDDS*TFVLAKV-LQFDQLFPVAIGEALGL 384
W PP G K N+DA +S TG + R + ++A L+ Q+ P + EAL L
Sbjct: 62 WEPPPEGIIKVNIDAGWSGSCS-TGFSLVARSHNAVMLVAATHLEETQIEP-TLAEALAL 235
Query: 385 YHAMQWMQDMYFDNIDFELDSKITRDAFY 413
+ +++M + I E S + + +
Sbjct: 236 RWCLSMIKEMEMERIIVESYSLVVLNTLH 322
>AV426428
Length = 413
Score = 33.1 bits (74), Expect = 0.086
Identities = 16/68 (23%), Positives = 29/68 (42%)
Frame = +3
Query: 133 IWYLKVSPKVKNLLWCMCRGCLPT*VRLQDKGVSCPTNCESCGADHEDLNHLLFECPFSI 192
+W + P+ W C G LP L +G++ C C A + +H + CP
Sbjct: 54 LWKAEALPRCCETGWRACLGVLPVKASLHARGLAEDPICPRCLAAPKTADHAVLFCP*VH 233
Query: 193 HVWNSAGI 200
+W ++ +
Sbjct: 234 PIWFASSL 257
>CB828953
Length = 554
Score = 32.3 bits (72), Expect = 0.15
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -3
Query: 326 WTPPSSGRFKCNVDAAFSAQFQRTGIGVCIRDDS 359
W PP K NVDA++ A IG+ +RD++
Sbjct: 540 WRPPLPRMLKLNVDASWKASSPLCSIGIAVRDEA 439
>TC11323
Length = 657
Score = 32.3 bits (72), Expect = 0.15
Identities = 27/88 (30%), Positives = 36/88 (40%), Gaps = 5/88 (5%)
Frame = +2
Query: 325 KWTPPSSGRFKCNVDAAFSAQFQRTGIGVCIRDDS*TFVLA-----KVLQFDQLFPVAIG 379
+W P GRFK N D + G G +RD S F+ V Q +L AI
Sbjct: 2 RWLKPVHGRFKLNFDGSSLGNRGNAGGGGLLRDGSSNFIFGFSIFLAVAQIMKLSLCAIL 181
Query: 380 EALGLYHAMQWMQDMYFDNIDFELDSKI 407
E L + + + +D I E DS I
Sbjct: 182 EGLLV------CKPLGYDGIGIECDSNI 247
>AV780445
Length = 504
Score = 32.3 bits (72), Expect = 0.15
Identities = 15/34 (44%), Positives = 18/34 (52%)
Frame = +1
Query: 325 KWTPPSSGRFKCNVDAAFSAQFQRTGIGVCIRDD 358
+W PP G K NVD + Q + G G IRDD
Sbjct: 388 RWQPPRQGTVKLNVDGSGREQDRCMGAGGLIRDD 489
Score = 31.6 bits (70), Expect = 0.25
Identities = 12/28 (42%), Positives = 15/28 (52%)
Frame = +2
Query: 174 CGADHEDLNHLLFECPFSIHVWNSAGIW 201
C A ED+ H L +CP S +W G W
Sbjct: 68 CPAPEEDI*HCLRDCPHSQEIWLRLGAW 151
>AV414192
Length = 160
Score = 30.8 bits (68), Expect = 0.43
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +3
Query: 88 DQVTNDCLVWKAERNGCYSVRSAYR 112
D DC VW+ + +G YS RS Y+
Sbjct: 21 DDSMTDCQVWRGDMSGLYSTRSGYQ 95
>BP056414
Length = 606
Score = 30.4 bits (67), Expect = 0.56
Identities = 16/32 (50%), Positives = 18/32 (56%)
Frame = -3
Query: 326 WTPPSSGRFKCNVDAAFSAQFQRTGIGVCIRD 357
WT P G FK NVD A S Q + GV +RD
Sbjct: 562 WTRPPEGWFKVNVDGALSGDLQASCGGV-VRD 470
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.326 0.138 0.461
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,057,308
Number of Sequences: 28460
Number of extensions: 150387
Number of successful extensions: 1355
Number of sequences better than 10.0: 67
Number of HSP's better than 10.0 without gapping: 1303
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1341
length of query: 413
length of database: 4,897,600
effective HSP length: 93
effective length of query: 320
effective length of database: 2,250,820
effective search space: 720262400
effective search space used: 720262400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC134322.1