BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC134049.5 + phase: 0
(162 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 48 7e-07
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 42 5e-05
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 40 3e-04
AV420911 40 3e-04
TC8302 37 0.001
BI418821 36 0.003
AV773507 36 0.003
AV421607 35 0.006
TC12773 32 0.041
TC17929 32 0.053
BP065763 31 0.091
TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, part... 29 0.35
TC18767 29 0.35
TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, par... 29 0.45
TC11158 similar to UP|Q39682 (Q39682) Glycine-rich protein (Frag... 29 0.45
AI967518 28 0.59
TC9034 27 1.3
BG662001 27 1.3
TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 27 1.3
TC11361 27 2.2
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 48.1 bits (113), Expect = 7e-07
Identities = 25/65 (38%), Positives = 34/65 (51%)
Frame = +2
Query: 63 RTKELTKSKDLRADEDNEGLSVSKGNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDCP 122
R +E + +D R D D S+G GG RG+ G +CFKC K GHF R+CP
Sbjct: 326 RYRERGRDRDDRGDRDR-----SRGYGGSRGSNGG---------ECFKCGKPGHFARECP 463
Query: 123 EDNES 127
+ E+
Sbjct: 464 SEGET 478
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 42.0 bits (97), Expect = 5e-05
Identities = 22/65 (33%), Positives = 30/65 (45%)
Frame = +1
Query: 57 EVQAALRTKELTKSKDLRADEDNEGLSVSKGNGGGRGNRGSSKSGNKDKYKCFKCHKLGH 116
+ Q A+R EL R + + G GGGRG S G KC++C + GH
Sbjct: 232 DAQDAIR--ELDGKNGWRVELSHNSRGGGGGGGGGRGGGRSGGGGGGSDMKCYECGEPGH 405
Query: 117 FKRDC 121
F R+C
Sbjct: 406 FAREC 420
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 39.7 bits (91), Expect = 3e-04
Identities = 19/37 (51%), Positives = 24/37 (64%)
Frame = +1
Query: 85 SKGNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDC 121
SKG GGGRG G G +D KC++C + GHF R+C
Sbjct: 106 SKGGGGGRG--GGRGRGGED-LKCYECGEPGHFAREC 207
>AV420911
Length = 418
Score = 39.7 bits (91), Expect = 3e-04
Identities = 19/37 (51%), Positives = 24/37 (64%)
Frame = +2
Query: 85 SKGNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDC 121
SKG GGGRG G G +D KC++C + GHF R+C
Sbjct: 314 SKGGGGGRG--GGRGRGGED-LKCYECGEPGHFAREC 415
>TC8302
Length = 494
Score = 37.4 bits (85), Expect = 0.001
Identities = 31/131 (23%), Positives = 57/131 (42%), Gaps = 4/131 (3%)
Frame = +3
Query: 2 EQLAEFNKILDDLENIEVQLEDEDKAILLLCALPRSFE----SFKDTMLYGKEGTVTLEE 57
+ + N + L + + ++A LLL +LP S++ + K+ + + L E
Sbjct: 48 DHIDHMNTLFAQLSASNFTIGENERAELLLESLPDSYDQLVINIKNNNIVNHLPLMMLSE 227
Query: 58 VQAALRTKELTKSKDLRADEDNEGLSVSKGNGGGRGNRGSSKSGNKDKYKCFKCHKLGHF 117
V ++ K + + E L+++ RG SKS K KC+ C + H
Sbjct: 228 VLLEEDSQR*NKEDR*DSSKPMEALTMT---------RGRSKSRKKINLKCYHCGQR*HL 380
Query: 118 KRDCPEDNESS 128
K+DC + +SS
Sbjct: 381 KKDCWFNKKSS 413
>BI418821
Length = 614
Score = 36.2 bits (82), Expect = 0.003
Identities = 23/76 (30%), Positives = 28/76 (36%), Gaps = 4/76 (5%)
Frame = +2
Query: 83 SVSKGNGGGRGN-RGSSKSGNKDKYKCFKCHKLGHFKRDCPEDNES---SAQVVSEEYGD 138
S +G GG RG R + G C+ C GH RDC N + GD
Sbjct: 314 SAPRGFGGWRGGERRNGGGGGGGGGGCYNCGDTGHLARDCHRSNNNGGGGGGAACYNCGD 493
Query: 139 AGALVVSCWEEDEGEG 154
AG L C + G
Sbjct: 494 AGHLARDCNRSNNNSG 541
Score = 33.5 bits (75), Expect = 0.018
Identities = 22/76 (28%), Positives = 28/76 (35%), Gaps = 16/76 (21%)
Frame = +2
Query: 87 GNGGGRG--------------NRGSSKSGNKDKYKCFKCHKLGHFKRDCPEDNESSAQVV 132
G GGG G +R ++ G C+ C GH RDC N +S
Sbjct: 371 GGGGGGGCYNCGDTGHLARDCHRSNNNGGGGGGAACYNCGDAGHLARDCNRSNNNSGGGG 550
Query: 133 SEEY--GDAGALVVSC 146
+ Y GD G L C
Sbjct: 551 AGCYNCGDTGHLARDC 598
Score = 28.9 bits (63), Expect = 0.45
Identities = 18/58 (31%), Positives = 21/58 (36%), Gaps = 17/58 (29%)
Frame = +2
Query: 85 SKGNGGGRG-----------------NRGSSKSGNKDKYKCFKCHKLGHFKRDCPEDN 125
S NGGG G NR ++ SG C+ C GH RDC N
Sbjct: 440 SNNNGGGGGGAACYNCGDAGHLARDCNRSNNNSGGGGA-GCYNCGDTGHLARDCNRSN 610
>AV773507
Length = 496
Score = 36.2 bits (82), Expect = 0.003
Identities = 17/47 (36%), Positives = 26/47 (55%)
Frame = +2
Query: 76 DEDNEGLSVSKGNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDCP 122
D+ ++G + GGRG+ G+ +D CF+C + GH RDCP
Sbjct: 260 DDADQGYRGGGYSSGGRGSYGAGDRVGQDD--CFECGRPGHRARDCP 394
>AV421607
Length = 245
Score = 35.0 bits (79), Expect = 0.006
Identities = 15/43 (34%), Positives = 24/43 (54%)
Frame = +3
Query: 86 KGNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDCPEDNESS 128
+G+G G G+ + +G C+KC + GH+ RDCP +S
Sbjct: 6 EGSGPGSGSGSGTATG------CYKCGRPGHWSRDCPSSAPNS 116
>TC12773
Length = 420
Score = 32.3 bits (72), Expect = 0.041
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = +2
Query: 85 SKGNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDC 121
S G +R S+K + Y+C+KC + GH DC
Sbjct: 278 SDGTWDHNRHRWSNKRSSLSTYECWKCQRPGHMAEDC 388
>TC17929
Length = 791
Score = 32.0 bits (71), Expect = 0.053
Identities = 15/55 (27%), Positives = 28/55 (50%), Gaps = 2/55 (3%)
Frame = +2
Query: 97 SSKSGNKDKYK--CFKCHKLGHFKRDCPEDNESSAQVVSEEYGDAGALVVSCWEE 149
+S N K++ C++C + GH R+CP+++ S ++ +V CW E
Sbjct: 23 TSNRPNDSKFRQTCYRCGESGHKMRNCPKEHSSGEKI----------*LVGCWAE 157
>BP065763
Length = 537
Score = 31.2 bits (69), Expect = 0.091
Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 5/50 (10%)
Frame = -1
Query: 79 NEGLSVSKGNGGGRGNRGSS----KSGNKDK-YKCFKCHKLGHFKRDCPE 123
++G S SK G + + K G K + C+ C K GHFK+ CP+
Sbjct: 483 HDGASXSKAKPGRKEQKKDQALKVKEGRIHKEHVCYFCKKAGHFKKYCPK 334
>TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (56%)
Length = 598
Score = 29.3 bits (64), Expect = 0.35
Identities = 23/91 (25%), Positives = 35/91 (38%), Gaps = 16/91 (17%)
Frame = +1
Query: 47 YGKEGTVTLEEVQAALRTKELTKSKDLR-----ADEDNEGLSVSKGNGGGRGNRGSSKSG 101
YG+ V ++ A + + + D R D D L V G RG+R
Sbjct: 160 YGRVRDVDMKRDYAFVEFSDPRDADDARYNLDGRDVDGSRLIVEFAKGVPRGSREGGGGR 339
Query: 102 NKDKY-----------KCFKCHKLGHFKRDC 121
++D+ +CF C GH+ RDC
Sbjct: 340 DRDREYMGRGPPPGSGRCFNCGIDGHWARDC 432
Score = 26.6 bits (57), Expect = 2.2
Identities = 14/53 (26%), Positives = 22/53 (41%), Gaps = 7/53 (13%)
Frame = +1
Query: 76 DEDNEGLSVSKGNGGGR-------GNRGSSKSGNKDKYKCFKCHKLGHFKRDC 121
D D E + G GR G+ K KC++C + GH +++C
Sbjct: 340 DRDREYMGRGPPPGSGRCFNCGIDGHWARDCKAGDWKNKCYRCGERGHIEKNC 498
>TC18767
Length = 1004
Score = 29.3 bits (64), Expect = 0.35
Identities = 18/66 (27%), Positives = 27/66 (40%), Gaps = 7/66 (10%)
Frame = +2
Query: 67 LTKSKDLRADEDN-----EGLSVSKGNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDC 121
+ K+KD D D+ G ++ + G N +D +CF C H R+C
Sbjct: 29 IDKNKDFILDGDSVPLYDRGYALGLTSADGSSNLDGGLEIIEDASRCFNCGSYNHSLREC 208
Query: 122 --PEDN 125
P DN
Sbjct: 209 SRPRDN 226
>TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (62%)
Length = 684
Score = 28.9 bits (63), Expect = 0.45
Identities = 29/111 (26%), Positives = 45/111 (40%), Gaps = 31/111 (27%)
Frame = +2
Query: 47 YGKEGTVTLEEVQAALRTKELTKSKDLRADEDNEGLSVSK-----GNGGGRGNR------ 95
YG+ V ++ A + + + D R + D + S+ GG RGNR
Sbjct: 170 YGRVRGVDMKHNFAFVEFSDPRDADDARYNLDGRDVEGSRIVVEFAKGGPRGNREYLGRG 349
Query: 96 ---GSSKSGN--------KD------KYKCFKCHKLGHFKRDC---PEDNE 126
GS + N +D K KC++C GH +R+C P+ NE
Sbjct: 350 PPPGSGRCFNCGLDGHWARDCKAGDWKNKCYRCGDRGHVERNCKNSPKKNE 502
>TC11158 similar to UP|Q39682 (Q39682) Glycine-rich protein (Fragment),
partial (22%)
Length = 357
Score = 28.9 bits (63), Expect = 0.45
Identities = 13/34 (38%), Positives = 21/34 (61%), Gaps = 2/34 (5%)
Frame = -1
Query: 74 RADEDNEGLSVSKGNGGG--RGNRGSSKSGNKDK 105
++DED G+ +S G GGG G G+ K G +++
Sbjct: 342 KSDEDGVGVDLSGGGGGG*RMGECGNEKEGEEEE 241
>AI967518
Length = 451
Score = 28.5 bits (62), Expect = 0.59
Identities = 12/32 (37%), Positives = 17/32 (52%)
Frame = +2
Query: 92 RGNRGSSKSGNKDKYKCFKCHKLGHFKRDCPE 123
RG R S K ++ + + C K GH R CP+
Sbjct: 11 RGGRPSKKRYSRTRRRRTNCRKTGHTLRTCPK 106
>TC9034
Length = 583
Score = 27.3 bits (59), Expect = 1.3
Identities = 12/54 (22%), Positives = 22/54 (40%)
Frame = +1
Query: 84 VSKGNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDCPEDNESSAQVVSEEYG 137
+++ G G S ++ + KC C + GH +C D + V+ G
Sbjct: 76 INRKRGVTEGPTVSHEARRSHRVKCANCEEFGHDSWECQRDKSKRQEKVTVRSG 237
>BG662001
Length = 213
Score = 27.3 bits (59), Expect = 1.3
Identities = 14/40 (35%), Positives = 19/40 (47%)
Frame = +1
Query: 77 EDNEGLSVSKGNGGGRGNRGSSKSGNKDKYKCFKCHKLGH 116
E + S SKG+ GGR + K N K++ KL H
Sbjct: 43 ESSSTTSESKGSHGGRVSAKGQKRKNHQKHRALPTVKLNH 162
>TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (26%)
Length = 555
Score = 27.3 bits (59), Expect = 1.3
Identities = 11/28 (39%), Positives = 14/28 (49%)
Frame = +2
Query: 95 RGSSKSGNKDKYKCFKCHKLGHFKRDCP 122
R S+ G C C + GHF R+CP
Sbjct: 305 RRDSRRGFSRDNLCKNCKRPGHFARECP 388
>TC11361
Length = 549
Score = 26.6 bits (57), Expect = 2.2
Identities = 12/24 (50%), Positives = 13/24 (54%)
Frame = +3
Query: 98 SKSGNKDKYKCFKCHKLGHFKRDC 121
SKS KD+ C C LGH K C
Sbjct: 12 SKSHKKDRPTCTHCGILGHTKDKC 83
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.310 0.132 0.373
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,320,246
Number of Sequences: 28460
Number of extensions: 26260
Number of successful extensions: 197
Number of sequences better than 10.0: 56
Number of HSP's better than 10.0 without gapping: 188
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 193
length of query: 162
length of database: 4,897,600
effective HSP length: 83
effective length of query: 79
effective length of database: 2,535,420
effective search space: 200298180
effective search space used: 200298180
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 52 (24.6 bits)
Medicago: description of AC134049.5