Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC134049.2 - phase: 0 
         (170 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC10599                                                                29  0.37
CN825072                                                               29  0.37
TC9078 similar to AAQ89615 (AAQ89615) At3g17205, partial (12%)         27  1.4
BP079392                                                               27  1.8
TC17469 similar to UP|Q8S8Z0 (Q8S8Z0) Protein phosphatase 2C, pa...    27  2.4
TC19832 similar to GB|AAO39903.1|28372842|BT003675 At5g67590 {Ar...    26  3.2
TC20229                                                                26  4.1
TC18381 similar to UP|Q9ASR8 (Q9ASR8) AT5g59960/mmn10_180, parti...    26  4.1
TC14349 UP|Q9M4R0 (Q9M4R0) Ubiquitin conjugating protein  (Ubiqu...    25  5.4
TC16129 similar to UP|AAR14275 (AAR14275) Predicted protein, par...    25  5.4
TC7911 similar to UP|Q39800 (Q39800) Delta-tonoplast intrinsic p...    25  5.4
BP085437                                                               25  5.4
TC11532 homologue to UP|Q8L5K5 (Q8L5K5) Ovule/fiber cell elongat...    25  5.4
TC15679                                                                25  7.0
AV781184                                                               25  7.0
BP045881                                                               25  9.2
BP032935                                                               25  9.2
TC16620 weakly similar to UP|Q9FN67 (Q9FN67) Gb|AAD56319.1, part...    25  9.2

>TC10599 
          Length = 600

 Score = 29.3 bits (64), Expect = 0.37
 Identities = 10/21 (47%), Positives = 18/21 (85%)
 Frame = +2

Query: 96  LCYLEGNAPMQRVLEVYDNYI 116
           LCY++GNAPM+ ++ +YD ++
Sbjct: 206 LCYIKGNAPMRSLVCLYDIFM 268


>CN825072 
          Length = 712

 Score = 29.3 bits (64), Expect = 0.37
 Identities = 17/44 (38%), Positives = 20/44 (44%), Gaps = 3/44 (6%)
 Frame = +2

Query: 51  SQHATCHVLQYECRFREAVEFMEE---CSPSWNSFLSFMLTHNW 91
           S HA      +  R R A    E    CSP+WNS  S + TH W
Sbjct: 338 SAHARAQRRSFVVRHRVATWQWERRSICSPAWNSNRSAIRTHPW 469


>TC9078 similar to AAQ89615 (AAQ89615) At3g17205, partial (12%)
          Length = 666

 Score = 27.3 bits (59), Expect = 1.4
 Identities = 11/22 (50%), Positives = 14/22 (63%)
 Frame = -3

Query: 38  AKRGFEINNQDGWSQHATCHVL 59
           AK+ F+I+    WS  ATCH L
Sbjct: 214 AKQRFKISKSKQWSARATCHKL 149


>BP079392 
          Length = 485

 Score = 26.9 bits (58), Expect = 1.8
 Identities = 11/17 (64%), Positives = 13/17 (75%)
 Frame = -3

Query: 129 EVYLNAVALLLRLCVRD 145
           E+YL+  AL L LCVRD
Sbjct: 465 EIYLSVCALFLSLCVRD 415


>TC17469 similar to UP|Q8S8Z0 (Q8S8Z0) Protein phosphatase 2C, partial
          (13%)
          Length = 568

 Score = 26.6 bits (57), Expect = 2.4
 Identities = 13/31 (41%), Positives = 21/31 (66%)
 Frame = +3

Query: 31 MKEAEEAAKRGFEINNQDGWSQHATCHVLQY 61
          +++AEEAAKR  +   Q G S + TC V+++
Sbjct: 15 IEDAEEAAKRLMQEAYQRGSSDNLTCVVVRF 107


>TC19832 similar to GB|AAO39903.1|28372842|BT003675 At5g67590 {Arabidopsis
           thaliana;}, partial (75%)
          Length = 530

 Score = 26.2 bits (56), Expect = 3.2
 Identities = 13/41 (31%), Positives = 20/41 (48%)
 Frame = +1

Query: 84  SFMLTHNWWHVALCYLEGNAPMQRVLEVYDNYIWKELDKTD 124
           +F   H W +V     + + P+ +V    DN+ WK L K D
Sbjct: 415 AFAEKHGWEYVVK---KPHTPLLKVKSYADNFKWKGLPKVD 528


>TC20229 
          Length = 666

 Score = 25.8 bits (55), Expect = 4.1
 Identities = 9/17 (52%), Positives = 14/17 (81%)
 Frame = -3

Query: 7   VLPQNEGENFIYGMLAF 23
           +LPQ++GE+ +Y  LAF
Sbjct: 433 LLPQDQGESLVYACLAF 383


>TC18381 similar to UP|Q9ASR8 (Q9ASR8) AT5g59960/mmn10_180, partial (53%)
          Length = 758

 Score = 25.8 bits (55), Expect = 4.1
 Identities = 18/59 (30%), Positives = 28/59 (46%)
 Frame = -3

Query: 107 RVLEVYDNYIWKELDKTDATVPEVYLNAVALLLRLCVRDELEFFGDRLKMLADRLADQV 165
           R+  +  NYI+  +    + V E  L  +A L R+C           L++L DRLA+ V
Sbjct: 513 RIFFLVHNYIFGSVINFQSYVGEELLQQIAPLRRICECP--------LELLVDRLAESV 361


>TC14349 UP|Q9M4R0 (Q9M4R0) Ubiquitin conjugating protein
           (Ubiquitin-conjugating enzyme E2) (Ubiquitin-protein
           ligase) (Ubiquitin carrier protein) , complete
          Length = 897

 Score = 25.4 bits (54), Expect = 5.4
 Identities = 12/37 (32%), Positives = 16/37 (42%)
 Frame = +3

Query: 60  QYECRFREAVEFMEECSPSWNSFLSFMLTHNWWHVAL 96
           Q EC  R +V  +EEC   W+        H +  V L
Sbjct: 534 QLECSVRTSVSTIEECGKLWSRVGQLTKAHLYMLVGL 644


>TC16129 similar to UP|AAR14275 (AAR14275) Predicted protein, partial (19%)
          Length = 599

 Score = 25.4 bits (54), Expect = 5.4
 Identities = 10/40 (25%), Positives = 19/40 (47%)
 Frame = +2

Query: 79  WNSFLSFMLTHNWWHVALCYLEGNAPMQRVLEVYDNYIWK 118
           WN    F+L   WW +   +++G   M+  + + +   WK
Sbjct: 359 WNVHHEFLL---WWSIK*LWMQGT*RMRMTINILECLSWK 469


>TC7911 similar to UP|Q39800 (Q39800) Delta-tonoplast intrinsic protein,
           complete
          Length = 1018

 Score = 25.4 bits (54), Expect = 5.4
 Identities = 15/53 (28%), Positives = 21/53 (39%), Gaps = 3/53 (5%)
 Frame = +3

Query: 50  WSQHATCHVLQYECRFREAVEFMEECSPSWNSFLSFML---THNWWHVALCYL 99
           W QH +C  + +     E    +  CS  W       L   T +WW +  CYL
Sbjct: 594 WCQHLSCRAILWWVN--EPSTLLWPCSCQW*LP*QLDLLGWTSHWWWLGWCYL 746


>BP085437 
          Length = 456

 Score = 25.4 bits (54), Expect = 5.4
 Identities = 10/40 (25%), Positives = 19/40 (47%)
 Frame = -1

Query: 79  WNSFLSFMLTHNWWHVALCYLEGNAPMQRVLEVYDNYIWK 118
           WN    F+L   WW +   +++G   M+  + + +   WK
Sbjct: 231 WNVHHEFLL---WWSIK*LWMQGT*RMRMTINILECLSWK 121


>TC11532 homologue to UP|Q8L5K5 (Q8L5K5) Ovule/fiber cell elongation protein
           Ghfe1, partial (24%)
          Length = 415

 Score = 25.4 bits (54), Expect = 5.4
 Identities = 13/39 (33%), Positives = 22/39 (56%), Gaps = 3/39 (7%)
 Frame = +3

Query: 15  NFIYGMLAFP---LLELGQMKEAEEAAKRGFEINNQDGW 50
           +F+ GM+  P   ++  G+    EEA K+G +IN+   W
Sbjct: 66  DFVEGMVYTPTEAVMMTGRYASKEEAKKKGNKINSVGWW 182


>TC15679 
          Length = 609

 Score = 25.0 bits (53), Expect = 7.0
 Identities = 10/27 (37%), Positives = 13/27 (48%)
 Frame = +3

Query: 91  WWHVALCYLEGNAPMQRVLEVYDNYIW 117
           WW V L  + GNA     + VY +  W
Sbjct: 252 WWEVMLDSVHGNAKTTGFVSVYYSLSW 332


>AV781184 
          Length = 462

 Score = 25.0 bits (53), Expect = 7.0
 Identities = 10/38 (26%), Positives = 14/38 (36%)
 Frame = +1

Query: 49  GWSQHATCHVLQYECRFREAVEFMEECSPSWNSFLSFM 86
           GW +     V    C     V  +EEC   W   + F+
Sbjct: 175 GWGRKPISEVYSLACNISHIVRELEECLDRWQFRVPFI 288


>BP045881 
          Length = 420

 Score = 24.6 bits (52), Expect = 9.2
 Identities = 14/39 (35%), Positives = 19/39 (47%), Gaps = 3/39 (7%)
 Frame = -1

Query: 24  PLLELGQMKEAEEAAKRGFEINNQDGWSQH---ATCHVL 59
           PL  LG+ KE  E  K+G+ +    GW  H     CH +
Sbjct: 225 PLRHLGEGKEVPEPWKKGWLLR---GWLLHTMPGDCHTM 118


>BP032935 
          Length = 534

 Score = 24.6 bits (52), Expect = 9.2
 Identities = 7/14 (50%), Positives = 10/14 (71%)
 Frame = +2

Query: 89  HNWWHVALCYLEGN 102
           H W ++  CYL+GN
Sbjct: 14  HGWCNMXYCYLQGN 55


>TC16620 weakly similar to UP|Q9FN67 (Q9FN67) Gb|AAD56319.1, partial (17%)
          Length = 518

 Score = 24.6 bits (52), Expect = 9.2
 Identities = 8/29 (27%), Positives = 17/29 (58%)
 Frame = -2

Query: 82  FLSFMLTHNWWHVALCYLEGNAPMQRVLE 110
           +L ++    WWHV+ C  E +  M+ +++
Sbjct: 109 WLEWVRACKWWHVSCCSCEESEMMKMLMK 23


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.323    0.137    0.436 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,271,400
Number of Sequences: 28460
Number of extensions: 45894
Number of successful extensions: 255
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 255
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 255
length of query: 170
length of database: 4,897,600
effective HSP length: 84
effective length of query: 86
effective length of database: 2,506,960
effective search space: 215598560
effective search space used: 215598560
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 52 (24.6 bits)


Medicago: description of AC134049.2