BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC133864.7 - phase: 0
(563 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV420310 252 1e-67
AV767505 128 2e-30
TC17988 similar to UP|Q9FKP3 (Q9FKP3) Similarity to auxin-indepe... 94 4e-24
CB826637 85 3e-17
TC16052 homologue to UP|Q9LIN9 (Q9LIN9) Similarity to the auxin-... 73 1e-13
BP069184 72 3e-13
BP072051 53 1e-07
TC15667 48 4e-06
AV420093 46 1e-05
AV409670 44 7e-05
BP042688 39 0.003
TC17443 37 0.007
BP053751 33 0.16
BP053522 31 0.47
TC9178 homologue to GB|AAB17725.1|1654142|BCU38470 small GTP-bin... 28 4.0
AV768748 27 6.7
TC16470 weakly similar to UP|AAQ89634 (AAQ89634) At1g14020, part... 27 6.7
TC14698 27 8.8
>AV420310
Length = 408
Score = 252 bits (643), Expect = 1e-67
Identities = 117/136 (86%), Positives = 132/136 (97%)
Frame = +1
Query: 341 RMEKDVWVRTGCLPGLTPEYDEVINNERIQRPELLTARSNMTYHQRKMAGLCPLNVMEVM 400
RMEKDVWVRTGCLPGL+PE+DE++NNERIQ+P+LLTARSNMTYH+RKMAGLCPL +EV
Sbjct: 1 RMEKDVWVRTGCLPGLSPEFDEIVNNERIQKPKLLTARSNMTYHERKMAGLCPLTAIEVT 180
Query: 401 RLLKALGAPKDARIYWAGGKPLGGKEALLPLIQEFPNFYNKEDLALPGELQPFVRKASLM 460
RLLKALGAP+++RIYWAGGKPLGGKEALLPLIQEFP+FYNKEDLALPGEL+PF +KASLM
Sbjct: 181 RLLKALGAPQNSRIYWAGGKPLGGKEALLPLIQEFPHFYNKEDLALPGELEPFAKKASLM 360
Query: 461 AAIDYIVSEKSDVFMP 476
AAID+IVSEKSDVFMP
Sbjct: 361 AAIDFIVSEKSDVFMP 408
>AV767505
Length = 536
Score = 128 bits (322), Expect = 2e-30
Identities = 58/65 (89%), Positives = 63/65 (96%)
Frame = -3
Query: 494 GHKKYITPNKRHMLPYFLNSSLSEEEFNRIIKELHQDSLGQPELRTNKNGRDVTKYPVPE 553
GHKKYITPNKRHMLPYFLNSSLSE++FNRI+KELHQDSLGQPE RT+K GRDVTKYPVPE
Sbjct: 531 GHKKYITPNKRHMLPYFLNSSLSEKDFNRIMKELHQDSLGQPEHRTSKAGRDVTKYPVPE 352
Query: 554 CMCND 558
CMCN+
Sbjct: 351 CMCNN 337
>TC17988 similar to UP|Q9FKP3 (Q9FKP3) Similarity to auxin-independent
growth promoter, partial (40%)
Length = 857
Score = 94.4 bits (233), Expect(2) = 4e-24
Identities = 67/193 (34%), Positives = 94/193 (47%), Gaps = 7/193 (3%)
Frame = +3
Query: 330 KSKGPYLALHLRMEKDVWVRTGCLPG-------LTPEYDEVINNERIQRPELLTARSNMT 382
K+ G ++ LHLR +KD+ + C G +Y +VI R+ S T
Sbjct: 63 KNAGKFVVLHLRFDKDMAAHSACDFGGGKAEKFALAKYRQVIWQGRL-------LNSQFT 221
Query: 383 YHQRKMAGLCPLNVMEVMRLLKALGAPKDARIYWAGGKPLGGKEALLPLIQEFPNFYNKE 442
+ + G CP+ EV LL ALG R+Y A K GG+ + L Q FP +K+
Sbjct: 222 DEELRSQGRCPMTPEEVGLLLAALGFDNSTRLYLASHKVYGGEARISTLRQLFPLMEDKK 401
Query: 443 DLALPGELQPFVRKASLMAAIDYIVSEKSDVFMPSHGGNMGRAIQGHRAFTGHKKYITPN 502
LA E KASL+AA+DY V SD+F+ + GNM A+ GHR + + K I PN
Sbjct: 402 SLASSYERFQIKGKASLLAALDYYVGLHSDIFISASPGNMHNAVVGHRTYL-NLKTIRPN 578
Query: 503 KRHMLPYFLNSSL 515
M FLN ++
Sbjct: 579 LVLMGQLFLNKTM 617
Score = 34.3 bits (77), Expect(2) = 4e-24
Identities = 16/38 (42%), Positives = 21/38 (55%)
Frame = +1
Query: 519 EFNRIIKELHQDSLGQPELRTNKNGRDVTKYPVPECMC 556
EF + E HQ+ GQ LR K + + YP P+CMC
Sbjct: 628 EFEHAVVEGHQNRQGQ--LRLRKPKQSIYTYPAPDCMC 735
>CB826637
Length = 571
Score = 85.1 bits (209), Expect = 3e-17
Identities = 44/135 (32%), Positives = 75/135 (54%), Gaps = 6/135 (4%)
Frame = +3
Query: 223 FGDIFDLEHFKRVLANDVRVVSALPSTHI-----MTRPVEGRPPLHATPSWIRARYLRRL 277
F I+D+EHF R L DV +V ++P + + + RPP A SW L+++
Sbjct: 6 FHGIYDVEHFIRTLRYDVNIVESIPESEKNGKKKKIKAFQIRPPRDAPISWYTTDALKKM 185
Query: 278 NREGVLLLRGLDSRLSKDLP-SDLQKLRCKVAFNALRFAEPIEELGNKIADRMKSKGPYL 336
G + L RL++++ + Q LRC+V ++ALRF I +L I D+++++GP++
Sbjct: 186 KEHGAIYLTPFSHRLAEEIDIPEFQTLRCRVNYHALRFKPHIMKLSQSIVDKLRAQGPFM 365
Query: 337 ALHLRMEKDVWVRTG 351
++HLR E D+ G
Sbjct: 366 SIHLRFEMDMLAFAG 410
>TC16052 homologue to UP|Q9LIN9 (Q9LIN9) Similarity to the auxin-independent
growth promoter, partial (24%)
Length = 809
Score = 72.8 bits (177), Expect = 1e-13
Identities = 47/130 (36%), Positives = 68/130 (52%), Gaps = 1/130 (0%)
Frame = +3
Query: 428 LLPLIQEFPNFYNKEDLALPGELQPFVRKASLMAAIDYIVSEKSDVFMPSHGGNMGRAIQ 487
+ PL FPN KE+LA EL F + + +AA+D++V KSDVF+ + GGN + I
Sbjct: 6 MAPLRNMFPNLVTKEELATKDELDGFRKHVTSLAALDFLVCLKSDVFVMTPGGNFAKLII 185
Query: 488 GHRAFTGHK-KYITPNKRHMLPYFLNSSLSEEEFNRIIKELHQDSLGQPELRTNKNGRDV 546
G R + GH+ K I P++ M F + + F + HQ G PE T N D+
Sbjct: 186 GARRYMGHRLKSIKPDQGLMSKSFGDPYMGWATFVEDVVVTHQTRTGLPE-ETFPN-YDL 359
Query: 547 TKYPVPECMC 556
+ P+ CMC
Sbjct: 360 WENPLTPCMC 389
>BP069184
Length = 505
Score = 71.6 bits (174), Expect = 3e-13
Identities = 45/116 (38%), Positives = 66/116 (56%), Gaps = 16/116 (13%)
Frame = -3
Query: 457 ASLMAAIDYIVSEKSDVFMPSHGGNMGRAIQGHRAFTGHKKYITPNKRHMLPYF---LNS 513
AS M A+DYIVS +SDVF+PS+ GNM RA++GHR F G + I+P+++ + F
Sbjct: 500 ASQMPALDYIVSIESDVFIPSYSGNMDRAVEGHRRFLGRGRTISPDRQALARLFDKLDQG 321
Query: 514 SLSE--EEFNRIIKELHQDSLGQPELRTN-----------KNGRDVTKYPVPECMC 556
+L+E E NRII +LH+ LG P R ++ P+P+C+C
Sbjct: 320 TLTEGKELSNRII-DLHRRRLGSPRKRKGPISGTKRMDRFRSEEPFYVNPLPDCLC 156
>BP072051
Length = 441
Score = 53.1 bits (126), Expect = 1e-07
Identities = 27/69 (39%), Positives = 37/69 (53%)
Frame = -3
Query: 390 GLCPLNVMEVMRLLKALGAPKDARIYWAGGKPLGGKEALLPLIQEFPNFYNKEDLALPGE 449
GLCPL E + +L ALG + IY AG GG+ L+ L +P +E+L P E
Sbjct: 433 GLCPLTPEEAILMLAALGFNRKTHIYVAGSNLYGGRSRLVALTSLYPKLVTQENLLSPDE 254
Query: 450 LQPFVRKAS 458
L+PF +S
Sbjct: 253 LKPFANYSS 227
>TC15667
Length = 750
Score = 48.1 bits (113), Expect = 4e-06
Identities = 26/81 (32%), Positives = 50/81 (61%)
Frame = +1
Query: 176 YLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPVLQVNVIWGDESEFGDIFDLEHFKRV 235
+++ ++ G +QI DAV++AR LGA LV+P ++ + G++ F DI+D++ F +
Sbjct: 385 FVIFSLTNGPEYHISQIADAVIVARSLGATLVIPDIRGSQP-GEKRNFEDIYDVDVFMKS 561
Query: 236 LANDVRVVSALPSTHIMTRPV 256
+ VRV LP+ H+ ++ +
Sbjct: 562 MDGVVRVAKELPA-HVSSQKI 621
>AV420093
Length = 215
Score = 46.2 bits (108), Expect = 1e-05
Identities = 24/68 (35%), Positives = 36/68 (52%), Gaps = 10/68 (14%)
Frame = +1
Query: 211 LQVNVIWGDESEFGDIFDLEHFKRVLANDVRVVSALPSTH---------IMTRPVE-GRP 260
L++N +W D S F DIFD++HF VL +D+ +V LP + I R
Sbjct: 1 LELNPVWRDSSSFMDIFDVDHFIDVLKDDISIVKELPEEYSWSTREYYAIAVRDTRIKNS 180
Query: 261 PLHATPSW 268
P+HA+ +W
Sbjct: 181 PVHASANW 204
>AV409670
Length = 283
Score = 43.9 bits (102), Expect = 7e-05
Identities = 26/88 (29%), Positives = 51/88 (57%), Gaps = 7/88 (7%)
Frame = +2
Query: 259 RPPLHATPSWIRARYLRRLNRE---GVLLLRGLDSRLSKDLP---SDLQKLRCKVAFNAL 312
+P A+P++ L +L + G+++ G L LP +++Q+LRC+VAF++L
Sbjct: 17 KPTSSASPNFYVKEVLPKLKKSKVVGIIIASG--GALQSALPPTMAEIQRLRCRVAFHSL 190
Query: 313 RFAEPIEELGNKIADRMKSKG-PYLALH 339
+F I+ LG+++ +++ G P+L H
Sbjct: 191 QFRPEIQMLGHRMIHKLRGLGQPFLVYH 274
>BP042688
Length = 480
Score = 38.5 bits (88), Expect = 0.003
Identities = 19/47 (40%), Positives = 28/47 (59%), Gaps = 2/47 (4%)
Frame = -1
Query: 471 SDVFMPSHGGNMGRAIQGHRAF--TGHKKYITPNKRHMLPYFLNSSL 515
S+VF+ + GGN + GHR F GH K I P+KR ++ F + S+
Sbjct: 480 SEVFVTTQGGNFPHFLMGHRRFLYDGHAKTIIPDKRKLVVLFEDMSI 340
>TC17443
Length = 531
Score = 37.4 bits (85), Expect = 0.007
Identities = 24/91 (26%), Positives = 44/91 (47%), Gaps = 6/91 (6%)
Frame = +2
Query: 435 FPNFYNKEDLALPGELQPFV--RKASLMAAIDYIVSEKSDVFMPSHGGNMGRAIQGHRAF 492
FP Y KE + + F+ + + L ID+ +S +SDVF+P+ G + + G R
Sbjct: 20 FPKTYTKEAIVPADKKSKFLDSQDSELEKVIDFYISSESDVFVPAISGLLYANVAGKRIG 199
Query: 493 TGHKKYIT----PNKRHMLPYFLNSSLSEEE 519
+G + + P+ FL+S + ++E
Sbjct: 200 SGKTQILVPADIPDSSASASSFLSSYIXQKE 292
>BP053751
Length = 507
Score = 32.7 bits (73), Expect = 0.16
Identities = 19/69 (27%), Positives = 34/69 (48%), Gaps = 2/69 (2%)
Frame = -2
Query: 435 FPNFYNKEDLALPGELQPFV--RKASLMAAIDYIVSEKSDVFMPSHGGNMGRAIQGHRAF 492
FP Y KE + + F+ + + + ID+ +S +SDVF+P+ G + G R
Sbjct: 500 FPKTYTKEAIMPADKKTKFLDSKDSEIEKVIDFYLSFQSDVFVPAISGLFYANVVGKRIG 321
Query: 493 TGHKKYITP 501
+G + + P
Sbjct: 320 SGKTQILVP 294
>BP053522
Length = 466
Score = 31.2 bits (69), Expect = 0.47
Identities = 16/56 (28%), Positives = 30/56 (53%)
Frame = -1
Query: 501 PNKRHMLPYFLNSSLSEEEFNRIIKELHQDSLGQPELRTNKNGRDVTKYPVPECMC 556
P+KR + F N +L + F + ++++ + S Q K G + +P+P+CMC
Sbjct: 466 PDKRKLAQLFDNPNLRWDLFKQQMRDMLRRS-DQKGTELKKAGDSLYTFPMPDCMC 302
>TC9178 homologue to GB|AAB17725.1|1654142|BCU38470 small GTP-binding
protein ARF {Brassica rapa subsp. pekinensis;} ,
complete
Length = 1033
Score = 28.1 bits (61), Expect = 4.0
Identities = 17/44 (38%), Positives = 22/44 (49%)
Frame = -2
Query: 250 HIMTRPVEGRPPLHATPSWIRARYLRRLNREGVLLLRGLDSRLS 293
H++ P P LH TP + Y LNR+ LLL L+S S
Sbjct: 615 HLLVLPP---PELHGTPP*LSQAYQCHLNRQHRLLLEYLESNTS 493
>AV768748
Length = 524
Score = 27.3 bits (59), Expect = 6.7
Identities = 18/47 (38%), Positives = 24/47 (50%)
Frame = +2
Query: 10 KLSYISVPSQIINSISSSSLQSLIDSPKKSSKTTIFTRKSTLWFLAL 56
KL+ SVP+ I+S ++ S QS K K TIF K FL +
Sbjct: 62 KLARASVPTSKIHSSNTYSQQSE*PHLKTKGKVTIFKSKMKSQFLCI 202
>TC16470 weakly similar to UP|AAQ89634 (AAQ89634) At1g14020, partial (10%)
Length = 765
Score = 27.3 bits (59), Expect = 6.7
Identities = 11/27 (40%), Positives = 16/27 (58%)
Frame = +3
Query: 512 NSSLSEEEFNRIIKELHQDSLGQPELR 538
N L+ +EF+ +KE H D +G P R
Sbjct: 15 NGVLNWDEFSSAVKESHADRMGSPSKR 95
>TC14698
Length = 674
Score = 26.9 bits (58), Expect = 8.8
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = -1
Query: 495 HKKYITPNKRHMLPYFLNSSL 515
H +ITPN R + P F NSSL
Sbjct: 371 HNLHITPNHRLLPPIFSNSSL 309
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.136 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,508,916
Number of Sequences: 28460
Number of extensions: 128699
Number of successful extensions: 705
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 695
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 700
length of query: 563
length of database: 4,897,600
effective HSP length: 95
effective length of query: 468
effective length of database: 2,193,900
effective search space: 1026745200
effective search space used: 1026745200
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC133864.7