BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC133572.2 - phase: 0
(365 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8728 similar to UP|ZIP4_ARATH (O04089) Zinc transporter 4, chl... 248 9e-67
TC11999 similar to UP|ZIP4_ARATH (O04089) Zinc transporter 4, ch... 231 1e-61
BP068821 119 7e-33
TC10899 similar to UP|CAE30488 (CAE30488) Zinc transporter ZIP6,... 130 3e-31
AV427146 114 3e-26
TC13070 similar to UP|AAR08416 (AAR08416) Metal transport protei... 101 2e-22
TC15814 similar to UP|AAR08414 (AAR08414) Metal transport protei... 53 7e-08
AV423942 52 2e-07
BF177474 47 4e-06
TC10944 35 0.015
TC10188 similar to UP|Q941N0 (Q941N0) Drought-induced protein, p... 31 0.29
BP084096 30 0.49
TC12836 GB|CAA71302.1|2292921|LJPANC pantoate--beta-alanine liga... 29 1.1
TC15910 similar to UP|Q9AR64 (Q9AR64) Urease accessory protein G... 28 2.4
AV773558 28 2.4
AV766647 28 2.4
TC14611 similar to UP|Q8LPB2 (Q8LPB2) Glycine-rich RNA binding p... 28 3.2
AV419295 27 4.1
TC13458 weakly similar to UP|Z282_HUMAN (Q9UDV7) Zinc finger pro... 27 4.1
TC11037 weakly similar to GB|AAP12885.1|30017303|BT006236 At3g12... 27 4.1
>TC8728 similar to UP|ZIP4_ARATH (O04089) Zinc transporter 4, chloroplast
precursor (ZRT/IRT-like protein 4), partial (35%)
Length = 521
Score = 248 bits (634), Expect = 9e-67
Identities = 122/131 (93%), Positives = 127/131 (96%)
Frame = +3
Query: 235 QSPCAIRPLIAALSFHQFFEGFALGGCISQAQFKASSTTIMACFFALTTPIGVGIGTGIA 294
QSPC IRPLIAALSFHQFFEGFALGGCISQAQFK SS TIMACFFALTTP+G+GIGTGIA
Sbjct: 3 QSPCTIRPLIAALSFHQFFEGFALGGCISQAQFKTSSATIMACFFALTTPVGIGIGTGIA 182
Query: 295 SVYNPYSPGALIAEGILDALSAGILVYMALVDLIAADFLSKRMSCNFRLQLVSYCMLFLG 354
SVYNPYS GAL+AEGILD+LSAGILVYMALVDLIAADFLSKRMSCNFRLQ+VSYCMLFLG
Sbjct: 183 SVYNPYSAGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCMLFLG 362
Query: 355 AGLMSSLAIWA 365
AGLMSSLAIWA
Sbjct: 363 AGLMSSLAIWA 395
>TC11999 similar to UP|ZIP4_ARATH (O04089) Zinc transporter 4, chloroplast
precursor (ZRT/IRT-like protein 4), partial (31%)
Length = 516
Score = 231 bits (590), Expect = 1e-61
Identities = 115/130 (88%), Positives = 122/130 (93%), Gaps = 2/130 (1%)
Frame = +2
Query: 1 MTNSTCGG--AESDLCRDEPAAFVLKFIAMASILLAGMAGIAIPLIGKHRRYLRTDGNLF 58
M ++ CGG A LCRD+ AAFVLKF+AMASILLAGMAGIAIPLIGKHRR+LRTDGNLF
Sbjct: 125 MNSTACGGGGAGEQLCRDDSAAFVLKFVAMASILLAGMAGIAIPLIGKHRRFLRTDGNLF 304
Query: 59 VAAKAFAAGVILATGFVHMLSDATEALNSPCLPEFPWSKFPFTGFFAMMAALFTLLLDFV 118
VAAKAFAAGVILATGFVHMLSDAT+ALNSPCLPEFPWSKFPFTGFFAM+AALFTLLLDFV
Sbjct: 305 VAAKAFAAGVILATGFVHMLSDATKALNSPCLPEFPWSKFPFTGFFAMVAALFTLLLDFV 484
Query: 119 GTQYYERKQG 128
GTQYYERKQG
Sbjct: 485 GTQYYERKQG 514
>BP068821
Length = 535
Score = 119 bits (298), Expect(3) = 7e-33
Identities = 56/92 (60%), Positives = 73/92 (78%)
Frame = -1
Query: 211 VVSQVLELGIVSHSVIIGLSLGVSQSPCAIRPLIAALSFHQFFEGFALGGCISQAQFKAS 270
++S+VLELGIV HS+IIG+SLG +QS I+PL+ ALSFH FFEG LGGCISQ++F++
Sbjct: 529 IISRVLELGIVVHSLIIGVSLGTAQSLDTIKPLLVALSFHPFFEGMGLGGCISQSKFESR 350
Query: 271 STTIMACFFALTTPIGVGIGTGIASVYNPYSP 302
+T IMA FF+ TTP G+ IG G++SVY SP
Sbjct: 349 ATAIMATFFSFTTPTGIAIGMGVSSVYEENSP 254
Score = 32.3 bits (72), Expect(3) = 7e-33
Identities = 14/21 (66%), Positives = 19/21 (89%)
Frame = -2
Query: 304 ALIAEGILDALSAGILVYMAL 324
ALI EG++++ SAGIL+YMAL
Sbjct: 249 ALIVEGVVNSSSAGILIYMAL 187
Score = 25.8 bits (55), Expect(3) = 7e-33
Identities = 12/42 (28%), Positives = 22/42 (51%)
Frame = -3
Query: 324 LVDLIAADFLSKRMSCNFRLQLVSYCMLFLGAGLMSSLAIWA 365
LVDL+AADF++ ++ + +L + + L + WA
Sbjct: 188 LVDLLAADFMNPKLQNHLKLHVGANISLLSRCSFYVCIGQWA 63
>TC10899 similar to UP|CAE30488 (CAE30488) Zinc transporter ZIP6, partial
(38%)
Length = 610
Score = 130 bits (327), Expect = 3e-31
Identities = 69/136 (50%), Positives = 96/136 (69%), Gaps = 3/136 (2%)
Frame = +3
Query: 233 VSQSPCAIRPLIAALSFHQFFEGFALGGCISQAQFKASSTTIMACFFALTTPIGVGIGTG 292
+SQ+ C IRPL+AAL+FHQ FEG LGGC +QA F ++ M F++TTP+G+ +G
Sbjct: 3 MSQNVCTIRPLVAALAFHQIFEGMGLGGCFAQAGFSFATMAYMCFMFSVTTPMGIMLGMA 182
Query: 293 IASV--YNPYSPGALIAEGILDALSAGILVYMALVDLIAADFL-SKRMSCNFRLQLVSYC 349
+ S+ + SP ALI EG+L ++S+GIL+YMALVDLIA DF +K M+ + RL+ VS+
Sbjct: 183 LFSLTGLDDSSPNALIMEGLLGSVSSGILIYMALVDLIAIDFFHNKLMNSDPRLKKVSFV 362
Query: 350 MLFLGAGLMSSLAIWA 365
L LG+ MS LA+WA
Sbjct: 363 ALTLGSASMSILALWA 410
>AV427146
Length = 428
Score = 114 bits (285), Expect = 3e-26
Identities = 59/122 (48%), Positives = 77/122 (62%)
Frame = +3
Query: 5 TCGGAESDLCRDEPAAFVLKFIAMASILLAGMAGIAIPLIGKHRRYLRTDGNLFVAAKAF 64
TC + D RD+ A K A+ SIL A G+ IPL+GK L + ++F KAF
Sbjct: 63 TCDQEDED--RDKTKALRYKIAALVSILFASAIGVCIPLLGKIIPALSPEKDIFFIIKAF 236
Query: 65 AAGVILATGFVHMLSDATEALNSPCLPEFPWSKFPFTGFFAMMAALFTLLLDFVGTQYYE 124
AAGVIL+TGF+H+L DA E L SPCL E PW FPFTGF AM A+ TL+L+ T Y++
Sbjct: 237 AAGVILSTGFIHVLPDAFENLTSPCLNEHPWGDFPFTGFVAMCTAMGTLMLETYATAYFQ 416
Query: 125 RK 126
++
Sbjct: 417 KQ 422
>TC13070 similar to UP|AAR08416 (AAR08416) Metal transport protein, partial
(25%)
Length = 460
Score = 101 bits (252), Expect = 2e-22
Identities = 50/88 (56%), Positives = 66/88 (74%)
Frame = +3
Query: 278 FFALTTPIGVGIGTGIASVYNPYSPGALIAEGILDALSAGILVYMALVDLIAADFLSKRM 337
FF++TTP G+ +G +++ Y SP ALI G+L+A SAG+LVYMALVDL+AADF+S R+
Sbjct: 6 FFSVTTPFGIALGMAMSTSYKENSPSALIIVGLLNASSAGLLVYMALVDLLAADFMSPRL 185
Query: 338 SCNFRLQLVSYCMLFLGAGLMSSLAIWA 365
+ +LQL SY +FLGAG MS LA WA
Sbjct: 186 QGSIKLQLKSYIAVFLGAGAMSLLAKWA 269
>TC15814 similar to UP|AAR08414 (AAR08414) Metal transport protein, partial
(14%)
Length = 655
Score = 53.1 bits (126), Expect = 7e-08
Identities = 30/51 (58%), Positives = 35/51 (67%)
Frame = +3
Query: 312 DALSAGILVYMALVDLIAADFLSKRMSCNFRLQLVSYCMLFLGAGLMSSLA 362
+A SAGI +YMAL DL+AADF+ RM RL+L S L LGAG MS LA
Sbjct: 6 NAASAGIGIYMALADLLAADFMIPRMQNRARLRLGSNLSLLLGAGCMSLLA 158
>AV423942
Length = 413
Score = 52.0 bits (123), Expect = 2e-07
Identities = 36/111 (32%), Positives = 54/111 (48%), Gaps = 4/111 (3%)
Frame = +2
Query: 1 MTNSTCGG--AESDLCRDEPAAFVLKFIAMASILLAGMAGIAIPL-IGKHRRYLRTDGNL 57
M +S C + + CRD AA LK +++ I + G++ P+ + + +
Sbjct: 77 MASSACVTDLSRASSCRDGAAAAHLKILSIFIIFITSAVGMSSPVFLARIFQGKPLYDRA 256
Query: 58 FVAAKAFAAGVILATGFVHMLSDATEAL-NSPCLPEFPWSKFPFTGFFAMM 107
V K FAAGVIL+T VH+L DA AL + PW FPF G ++
Sbjct: 257 VVLIKCFAAGVILSTSLVHVLPDAYAALADCHVASRHPWRDFPFAGLVTLV 409
>BF177474
Length = 327
Score = 47.4 bits (111), Expect = 4e-06
Identities = 23/65 (35%), Positives = 38/65 (58%)
Frame = +3
Query: 6 CGGAESDLCRDEPAAFVLKFIAMASILLAGMAGIAIPLIGKHRRYLRTDGNLFVAAKAFA 65
C ++D C ++ A LK IA+ +IL++ + G+ PL+ + + NLF+ K FA
Sbjct: 129 CATEKADSCVNKEKAKPLKIIAIITILVSSIIGVCSPLLTRSIPAFSPESNLFIIVKCFA 308
Query: 66 AGVIL 70
AG+IL
Sbjct: 309 AGIIL 323
>TC10944
Length = 512
Score = 35.4 bits (80), Expect = 0.015
Identities = 19/52 (36%), Positives = 30/52 (57%), Gaps = 1/52 (1%)
Frame = +1
Query: 146 NVGKVFGEEESGGMHIVGMHAHAAHHRHNHPHGDACD-GGGIVKEHGHDHSH 196
++ ++F E +S G +H H+ H ++H HGDA D G G + +G HSH
Sbjct: 37 HIQEMFDEAKSNGHDHDHLHHHSHGHGYDH-HGDAHDHGAGYMDAYGLGHSH 189
Score = 30.4 bits (67), Expect = 0.49
Identities = 14/38 (36%), Positives = 15/38 (38%)
Frame = -2
Query: 159 MHIVGMHAHAAHHRHNHPHGDACDGGGIVKEHGHDHSH 196
MH HH +H H CDG E HDH H
Sbjct: 169 MHPYNQRHGREHHHGDHTHDHDCDG-----EDDHDHDH 71
>TC10188 similar to UP|Q941N0 (Q941N0) Drought-induced protein, partial
(84%)
Length = 747
Score = 31.2 bits (69), Expect = 0.29
Identities = 26/106 (24%), Positives = 37/106 (34%), Gaps = 25/106 (23%)
Frame = +3
Query: 116 DFVGTQYYERKQGMNRAVDEQARVGTSEEGNVGKVFGEEESGGMHIVGMHAHAAHHRHNH 175
D+ G Y +G + + + G G G E+ G H +G H H+ H
Sbjct: 174 DYKGEHYGGEHKGEHYGGEHKGE----SHGIGGFGHGGEQKGESHGIGGFGHGGEHKPEH 341
Query: 176 P----------------HGDACDGGGIVK---------EHGHDHSH 196
HGD +G G+ K EHGH+H H
Sbjct: 342 HGQENKEGFLDKVKDKIHGD--EGEGVEKKKKKDRKKHEHGHEHGH 473
>BP084096
Length = 122
Score = 30.4 bits (67), Expect = 0.49
Identities = 13/16 (81%), Positives = 15/16 (93%)
Frame = -3
Query: 350 MLFLGAGLMSSLAIWA 365
+LFLGAG MSSLA+WA
Sbjct: 120 LLFLGAGSMSSLAMWA 73
>TC12836 GB|CAA71302.1|2292921|LJPANC pantoate--beta-alanine ligase {Lotus
corniculatus var. japonicus;} , complete
Length = 1310
Score = 29.3 bits (64), Expect = 1.1
Identities = 16/47 (34%), Positives = 20/47 (42%), Gaps = 3/47 (6%)
Frame = -1
Query: 149 KVFGEEESGGMHIVGMHAHAA---HHRHNHPHGDACDGGGIVKEHGH 192
K + S H H H HHRH+H + C GG K+H H
Sbjct: 419 KFHAQNHSSYQHKTPPHHHHTPPQHHRHHHRNHTNCGGG---KQHQH 288
>TC15910 similar to UP|Q9AR64 (Q9AR64) Urease accessory protein G, partial
(88%)
Length = 1061
Score = 28.1 bits (61), Expect = 2.4
Identities = 15/44 (34%), Positives = 20/44 (45%)
Frame = +2
Query: 163 GMHAHAAHHRHNHPHGDACDGGGIVKEHGHDHSHALIAANEETD 206
G H H HH H+H D G H + HSH+ A+ +D
Sbjct: 11 GDHNHEHHHHHHHS-----DHGHDHDHHDNSHSHSDGVASAGSD 127
>AV773558
Length = 469
Score = 28.1 bits (61), Expect = 2.4
Identities = 10/28 (35%), Positives = 12/28 (42%)
Frame = +3
Query: 165 HAHAAHHRHNHPHGDACDGGGIVKEHGH 192
H H HH H+HPH + H H
Sbjct: 330 HHHHHHHHHHHPHYTPTQNHHPIPHHPH 413
>AV766647
Length = 349
Score = 28.1 bits (61), Expect = 2.4
Identities = 9/18 (50%), Positives = 10/18 (55%)
Frame = -3
Query: 167 HAAHHRHNHPHGDACDGG 184
H HH H+H HG C G
Sbjct: 320 HHHHHHHHHEHGGGCCSG 267
Score = 26.2 bits (56), Expect = 9.2
Identities = 10/23 (43%), Positives = 11/23 (47%), Gaps = 4/23 (17%)
Frame = -3
Query: 165 HAHAAHHRHNHPH----GDACDG 183
H H HH H+H H G C G
Sbjct: 335 HPHPHHHHHHHHHHEHGGGCCSG 267
>TC14611 similar to UP|Q8LPB2 (Q8LPB2) Glycine-rich RNA binding protein,
partial (20%)
Length = 760
Score = 27.7 bits (60), Expect = 3.2
Identities = 17/47 (36%), Positives = 20/47 (42%)
Frame = -2
Query: 171 HRHNHPHGDACDGGGIVKEHGHDHSHALIAANEETDVRHVVVSQVLE 217
H H+ GDAC G H H SH L + E + H Q LE
Sbjct: 384 HHHSSCDGDAC--GDASSSHHHHQSHHLHISYEHSPHYHHHHHQALE 250
>AV419295
Length = 383
Score = 27.3 bits (59), Expect = 4.1
Identities = 16/63 (25%), Positives = 23/63 (36%), Gaps = 7/63 (11%)
Frame = +2
Query: 167 HAAHHRHNHPHGDACDGGGIVKEHGHDHSH-------ALIAANEETDVRHVVVSQVLELG 219
H+ H+ H H +GG H H H H L+ E+ + S L
Sbjct: 95 HSLTHKQTHTHTCDMEGGDDFHHHFHHHHHHRQNFPLQLLEKKEDQEAASCSPSSPSSLA 274
Query: 220 IVS 222
I+S
Sbjct: 275 IIS 283
>TC13458 weakly similar to UP|Z282_HUMAN (Q9UDV7) Zinc finger protein 282
(HTLV-I U5RE binding protein 1) (HUB-1), partial (3%)
Length = 810
Score = 27.3 bits (59), Expect = 4.1
Identities = 12/30 (40%), Positives = 12/30 (40%)
Frame = +3
Query: 167 HAAHHRHNHPHGDACDGGGIVKEHGHDHSH 196
H HHR NH H C V H H H
Sbjct: 315 HHRHHRRNHHHHQQC----TVPHHRRHHHH 392
>TC11037 weakly similar to GB|AAP12885.1|30017303|BT006236 At3g12920
{Arabidopsis thaliana;}, partial (27%)
Length = 730
Score = 27.3 bits (59), Expect = 4.1
Identities = 13/44 (29%), Positives = 18/44 (40%)
Frame = +3
Query: 138 RVGTSEEGNVGKVFGEEESGGMHIVGMHAHAAHHRHNHPHGDAC 181
RVG S G+ F H + H+H +H+H H C
Sbjct: 180 RVGNSLPGSAAVRFLHRRFARRHEI*QHSHLQPPQHHHRHQQRC 311
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.325 0.139 0.418
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,381,020
Number of Sequences: 28460
Number of extensions: 93684
Number of successful extensions: 1197
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 992
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1108
length of query: 365
length of database: 4,897,600
effective HSP length: 91
effective length of query: 274
effective length of database: 2,307,740
effective search space: 632320760
effective search space used: 632320760
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC133572.2