BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC133139.2 + phase: 0 /pseudo
(155 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV766619 50 2e-07
BP030148 47 1e-06
TC12653 similar to UP|BIOB_ARATH (P54967) Biotin synthase (Biot... 30 0.18
TC11794 similar to AAP86662 (AAP86662) 26S proteasome subunit RP... 29 0.32
TC16381 27 1.6
TC14755 similar to UP|P93774 (P93774) Shaggy-like kinase (Fragme... 27 2.0
TC9042 weakly similar to PIR|G86367|G86367 protein F28C11.3 [imp... 26 2.7
AV771553 25 4.6
TC19323 homologue to UP|Q9XH50 (Q9XH50) 1-D-deoxyxylulose 5-phos... 25 4.6
AV409798 25 4.6
TC13923 homologue to UP|Q38950 (Q38950) Protein phosphatase 2A 6... 25 5.9
TC9581 UP|Q9SCA1 (Q9SCA1) Calcium-binding protein, complete 25 5.9
TC12045 similar to UP|Q9FT86 (Q9FT86) Peroxin Pex14, partial (5%) 25 5.9
BP075735 25 5.9
TC8081 similar to UP|AAQ84334 (AAQ84334) Zinc-finger protein, pa... 25 7.8
TC11366 25 7.8
TC8504 weakly similar to UP|Q8LG02 (Q8LG02) Nitrate transporter,... 25 7.8
>AV766619
Length = 412
Score = 50.1 bits (118), Expect = 2e-07
Identities = 24/123 (19%), Positives = 57/123 (45%)
Frame = +2
Query: 12 TPQPDLGLISDRHESIKSVVRRLDSNWHHVFCIRHITQLFEDFQEHRDEEKSKKYGLNNI 71
T +P + +++ +K + + HH +C+R++ + + + +++++ +N+
Sbjct: 8 TSEP-ITFVANCQNGLKKSLSEIFEKCHHSYCLRYLAEKLNKDLKGQFSHEARRFMIND- 181
Query: 72 NFLPSGYSMTQPSITHYRNQIRDWSDDVVKWVDDNPKKQWLQAHDQGRRWGHITTNISEC 131
F + Y+ Q + ++ S + WV N + W A G R+ H+T+N +
Sbjct: 182 -FYAAAYAPKQETFERSVENMKGISSEAFNWVMQNEPEHWANAFFNGARYNHLTSNFGQQ 358
Query: 132 FNN 134
F N
Sbjct: 359 FYN 367
>BP030148
Length = 398
Score = 47.4 bits (111), Expect = 1e-06
Identities = 33/117 (28%), Positives = 53/117 (45%), Gaps = 1/117 (0%)
Frame = +3
Query: 15 PDLGLISDRHESIKSVVRRLDSNWHHVFCIRHITQLFEDFQEHRDEEKSKKYGLNNINFL 74
P L ++SDR + I V H FC+RH++ F R E + +N L
Sbjct: 15 PRLTILSDRQKGIVDGVEANFPTAFHGFCMRHLSDSF------RKEFNNTML----VNLL 164
Query: 75 -PSGYSMTQPSITHYRNQIRDWSDDVVKWVDDNPKKQWLQAHDQGRRWGHITTNISE 130
+ + +T +I + S D W+ P + W A+ +G+R+GH+T NI E
Sbjct: 165 WEAAHVLTVIEFESKILEIEEISQDAAYWIRRIPPRLWATAYFEGQRFGHLTANIVE 335
>TC12653 similar to UP|BIOB_ARATH (P54967) Biotin synthase (Biotin
synthetase) , partial (29%)
Length = 535
Score = 30.0 bits (66), Expect = 0.18
Identities = 21/77 (27%), Positives = 32/77 (41%), Gaps = 5/77 (6%)
Frame = +1
Query: 78 YSMTQPSITHYRNQIR-----DWSDDVVKWVDDNPKKQWLQAHDQGRRWGHITTNISECF 132
YS + P+ I+ DW+ D VK + D+P L Q R H + +C
Sbjct: 202 YSSSSPAAIQAERTIKQGPRNDWTRDEVKSIYDSPVLDLLFHGAQVHRHAHNFREVQQCT 381
Query: 133 NNVLKGGPQSSNHTNCP 149
+K G S + + CP
Sbjct: 382 LLSVKTGGCSEDCSYCP 432
>TC11794 similar to AAP86662 (AAP86662) 26S proteasome subunit RPN6a,
partial (19%)
Length = 435
Score = 29.3 bits (64), Expect = 0.32
Identities = 11/24 (45%), Positives = 15/24 (61%)
Frame = -2
Query: 99 VVKWVDDNPKKQWLQAHDQGRRWG 122
++ W +D K WLQ +QGRR G
Sbjct: 167 ILWWQEDKMMKTWLQTPEQGRRGG 96
>TC16381
Length = 655
Score = 26.9 bits (58), Expect = 1.6
Identities = 12/26 (46%), Positives = 18/26 (69%), Gaps = 3/26 (11%)
Frame = -1
Query: 96 SDDVVKWVDDNPKKQWLQA---HDQG 118
S+D +K + D PK +WLQ+ H+QG
Sbjct: 271 SEDKLKQLLDQPKNKWLQSLTLHEQG 194
>TC14755 similar to UP|P93774 (P93774) Shaggy-like kinase (Fragment),
partial (12%)
Length = 653
Score = 26.6 bits (57), Expect = 2.0
Identities = 11/32 (34%), Positives = 17/32 (52%), Gaps = 1/32 (3%)
Frame = +2
Query: 16 DLGLISDRHESIKSVV-RRLDSNWHHVFCIRH 46
D L +H + K + R+L WHH FC+ +
Sbjct: 86 DYNLFMCQHLNDKQMA*RKLKQKWHHYFCLSY 181
>TC9042 weakly similar to PIR|G86367|G86367 protein F28C11.3 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(12%)
Length = 768
Score = 26.2 bits (56), Expect = 2.7
Identities = 10/22 (45%), Positives = 13/22 (58%)
Frame = +2
Query: 106 NPKKQWLQAHDQGRRWGHITTN 127
+P K WL H Q R H+TT+
Sbjct: 212 SPVKPWLTVHTQSPRAPHVTTS 277
>AV771553
Length = 454
Score = 25.4 bits (54), Expect = 4.6
Identities = 12/35 (34%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +1
Query: 11 VTPQPDLGLISDRHESIKSVVRRLDSNW-HHVFCI 44
VT G + + K+ +RRL +NW H+ CI
Sbjct: 343 VTHLKSSGSTASHSSNFKTKIRRLCNNWQQHIICI 447
>TC19323 homologue to UP|Q9XH50 (Q9XH50) 1-D-deoxyxylulose 5-phosphate
synthase, partial (15%)
Length = 530
Score = 25.4 bits (54), Expect = 4.6
Identities = 18/67 (26%), Positives = 30/67 (43%), Gaps = 2/67 (2%)
Frame = +3
Query: 36 SNWHHVFCIRHITQLFEDFQEHRDEEKSK--KYGLNNINFLPSGYSMTQPSITHYRNQIR 93
S+W + I H + FE ++ + K ++F+P Y+ S T Y +IR
Sbjct: 237 SSWSYT--ISHSSNSFEHAWTNKRGTRGHVMKENATGMSFIPPSYTE*HDSFTLYNTEIR 410
Query: 94 DWSDDVV 100
D S +V
Sbjct: 411 DVSIKLV 431
>AV409798
Length = 411
Score = 25.4 bits (54), Expect = 4.6
Identities = 12/62 (19%), Positives = 20/62 (31%)
Frame = +2
Query: 30 VVRRLDSNWHHVFCIRHITQLFEDFQEHRDEEKSKKYGLNNINFLPSGYSMTQPSITHYR 89
V + + W FC R D Q +NF G + + P + +
Sbjct: 26 VSENMGTGWRRAFCTRDPESTLSDKQHSSHSPSPSPRSCARLNFFSGGSNPSTPRLQQQQ 205
Query: 90 NQ 91
+Q
Sbjct: 206SQ 211
>TC13923 homologue to UP|Q38950 (Q38950) Protein phosphatase 2A 65 kDa
regulatory subunit, partial (20%)
Length = 515
Score = 25.0 bits (53), Expect = 5.9
Identities = 10/33 (30%), Positives = 17/33 (51%), Gaps = 2/33 (6%)
Frame = -2
Query: 78 YSMTQPSITHYRNQIRDWSD--DVVKWVDDNPK 108
+ + + ++ R W D+VKWV +NPK
Sbjct: 112 FXFGETKLQRRESRYRKWERVVDLVKWVSENPK 14
>TC9581 UP|Q9SCA1 (Q9SCA1) Calcium-binding protein, complete
Length = 1049
Score = 25.0 bits (53), Expect = 5.9
Identities = 10/45 (22%), Positives = 21/45 (46%)
Frame = +3
Query: 25 ESIKSVVRRLDSNWHHVFCIRHITQLFEDFQEHRDEEKSKKYGLN 69
+ + ++ R+D N I +L++ + RDEE+ + N
Sbjct: 453 KELTQMIERIDVNGDGCVDIDEFGELYQSIMDERDEEEDMREAFN 587
>TC12045 similar to UP|Q9FT86 (Q9FT86) Peroxin Pex14, partial (5%)
Length = 609
Score = 25.0 bits (53), Expect = 5.9
Identities = 12/27 (44%), Positives = 16/27 (58%)
Frame = +1
Query: 2 SQAPDLRLWVTPQPDLGLISDRHESIK 28
SQ P R+WV PQP +S E+I+
Sbjct: 196 SQQPVQRVWVPPQPPPVAMSAAAEAIR 276
>BP075735
Length = 482
Score = 25.0 bits (53), Expect = 5.9
Identities = 13/41 (31%), Positives = 18/41 (43%), Gaps = 11/41 (26%)
Frame = -2
Query: 23 RHESIKSVVRRLDS-----------NWHHVFCIRHITQLFE 52
RH + ++ R+D W HVFC+ HI FE
Sbjct: 124 RHRRSRILIFRIDGLGVMLNLYAF*RWVHVFCVFHIDGGFE 2
>TC8081 similar to UP|AAQ84334 (AAQ84334) Zinc-finger protein, partial
(65%)
Length = 1222
Score = 24.6 bits (52), Expect = 7.8
Identities = 8/18 (44%), Positives = 12/18 (66%)
Frame = +2
Query: 40 HVFCIRHITQLFEDFQEH 57
H+FC R + ++ FQEH
Sbjct: 974 HLFCFRMVFAIYR*FQEH 1027
>TC11366
Length = 586
Score = 24.6 bits (52), Expect = 7.8
Identities = 14/33 (42%), Positives = 16/33 (48%)
Frame = -1
Query: 57 HRDEEKSKKYGLNNINFLPSGYSMTQPSITHYR 89
H + K K L N+ LPS PSITH R
Sbjct: 430 HFNINK*NKTFLKNLLALPSSPQQCVPSITHVR 332
>TC8504 weakly similar to UP|Q8LG02 (Q8LG02) Nitrate transporter, partial
(9%)
Length = 493
Score = 24.6 bits (52), Expect = 7.8
Identities = 9/30 (30%), Positives = 18/30 (60%)
Frame = +1
Query: 16 DLGLISDRHESIKSVVRRLDSNWHHVFCIR 45
+L L+ + S+ S +L WHH++C++
Sbjct: 223 NLELMERKKHSLYSFHPKLILLWHHIWCVK 312
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.135 0.441
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,725,033
Number of Sequences: 28460
Number of extensions: 56986
Number of successful extensions: 271
Number of sequences better than 10.0: 35
Number of HSP's better than 10.0 without gapping: 268
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 271
length of query: 155
length of database: 4,897,600
effective HSP length: 83
effective length of query: 72
effective length of database: 2,535,420
effective search space: 182550240
effective search space used: 182550240
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)
Medicago: description of AC133139.2