BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC130807.4 - phase: 0
(329 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AU089582 103 2e-39
AV427422 133 4e-32
TC19412 similar to UP|Q84KB0 (Q84KB0) Pol protein, partial (7%) 96 4e-27
BP055130 105 7e-25
BP054462 92 1e-19
BF177840 89 1e-18
BP046863 60 7e-10
TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase regi... 55 2e-08
BP085845 43 8e-05
TC11563 29 1.2
TC16411 similar to GB|AAP31965.1|30387599|BT006621 At4g21540 {Ar... 27 4.7
BP072713 27 6.1
TC12763 similar to UP|CADH_MEDSA (P31656) Cinnamyl-alcohol dehyd... 26 8.0
BP033788 26 8.0
>AU089582
Length = 383
Score = 103 bits (258), Expect(2) = 2e-39
Identities = 49/83 (59%), Positives = 64/83 (77%)
Frame = +2
Query: 40 AQLAEIYIHNIVKLHGVPSSIVSDRDPRFTSRFWKSLQDALGSKLRLSSAYHPQTDGQSK 99
+Q A+IY+ IV LHGVP SI+SDR +FTS FW+S Q ALG++L++S+A+HPQTDGQS+
Sbjct: 14 SQYAKIYLDEIVSLHGVPVSIISDRGAQFTSHFWRSFQTALGTRLKMSTAFHPQTDGQSE 193
Query: 100 RTIQSLEYLLRVCVLEEGGAWDS 122
RTIQ LE +LR CV + G S
Sbjct: 194 RTIQILEDMLRACVXDLRGXLGS 262
Score = 75.5 bits (184), Expect(2) = 2e-39
Identities = 32/45 (71%), Positives = 38/45 (84%)
Frame = +3
Query: 118 GAWDSHLPLIEFTYNNSYHSSIGMAPFKALYGRRCRTPLCWFESG 162
G+WD +L L+EF YNNSY SSI MAPF+ALYGRRCR+P+ WFE G
Sbjct: 249 GSWDQYLSLMEFAYNNSYRSSI*MAPFEALYGRRCRSPIGWFEVG 383
>AV427422
Length = 417
Score = 133 bits (335), Expect = 4e-32
Identities = 60/133 (45%), Positives = 90/133 (67%)
Frame = +1
Query: 11 TRGHDSIWVVVDRLRKSAHFIPINIKYPVAQLAEIYIHNIVKLHGVPSSIVSDRDPRFTS 70
++G++++ VVVDRL K +HF+P+ Y +A+I++ +V+LHGVP SIVSDRDP F S
Sbjct: 19 SKGYEAVLVVVDRLSKFSHFVPLKHPYTAKVIADIFVREVVRLHGVPLSIVSDRDPLFMS 198
Query: 71 RFWKSLQDALGSKLRLSSAYHPQTDGQSKRTIQSLEYLLRVCVLEEGGAWDSHLPLIEFT 130
FWK L G+KL++S+AYHP++DGQ++ + LE LR + ++ +W +P E+
Sbjct: 199 NFWKELFKMQGTKLKMSTAYHPESDGQTEVVNRCLETYLRCFIADQPKSWAHWVPWAEYW 378
Query: 131 YNNSYHSSIGMAP 143
YN SYH S G P
Sbjct: 379 YNTSYHVSTGQTP 417
>TC19412 similar to UP|Q84KB0 (Q84KB0) Pol protein, partial (7%)
Length = 519
Score = 96.3 bits (238), Expect(2) = 4e-27
Identities = 46/79 (58%), Positives = 61/79 (76%)
Frame = +3
Query: 215 RVTPMTGVGHALKSRKLTPKFIGPYQISERVGTVAYRVGLPPHLSNLHDVFHVSQLRKYV 274
RV+PM GV K KL+P+FIGP+++ ERVG+V+YR+ LPP LS +H VFHVS LRKY+
Sbjct: 3 RVSPMKGVLRFGKKGKLSPRFIGPFEVLERVGSVSYRLALPPDLSAVHPVFHVSMLRKYL 182
Query: 275 ADPSHVIPRDDVQVRDNLT 293
DPSHVI +DVQ+ +L+
Sbjct: 183 YDPSHVIRHEDVQLDVHLS 239
Score = 41.6 bits (96), Expect(2) = 4e-27
Identities = 17/29 (58%), Positives = 20/29 (68%)
Frame = +2
Query: 300 VKVVWDGATGESLTWELESKMRDSYPKLF 328
VKV+W G +GE TWE E MR+ YP LF
Sbjct: 311 VKVLWRGPSGEEATWEAEDIMREKYPHLF 397
>BP055130
Length = 567
Score = 105 bits (263), Expect(2) = 7e-25
Identities = 56/112 (50%), Positives = 69/112 (61%)
Frame = +2
Query: 13 GHDSIWVVVDRLRKSAHFIPINIKYPVAQLAEIYIHNIVKLHGVPSSIVSDRDPRFTSRF 72
G I VVVDRL K HF P Y +Q+AE ++ IVKLHG+P +IVSDRD FTS F
Sbjct: 179 GFTVIIVVVDRLSKYGHFAPHRANYTSSQVAETFVSTIVKLHGMPRAIVSDRDKAFTSAF 358
Query: 73 WKSLQDALGSKLRLSSAYHPQTDGQSKRTIQSLEYLLRVCVLEEGGAWDSHL 124
WK G+ L +SS+YHPQTDGQ++ + LE LR V E W S+L
Sbjct: 359 WKHFFKLHGTTLNMSSSYHPQTDGQTEALNKCLELYLRCFVHETPRLWVSYL 514
Score = 24.3 bits (51), Expect(2) = 7e-25
Identities = 9/17 (52%), Positives = 13/17 (75%)
Frame = +3
Query: 124 LPLIEFTYNNSYHSSIG 140
LP E+ YN+S+ +SIG
Sbjct: 516 LPWAEYCYNSSFQTSIG 566
>BP054462
Length = 422
Score = 92.0 bits (227), Expect = 1e-19
Identities = 53/141 (37%), Positives = 81/141 (56%), Gaps = 1/141 (0%)
Frame = +1
Query: 132 NNSYHSSIGMAPFKALYGRRCRTPLCWFESGESVVLGPDLVHETTEKVKMIRKKMKASQS 191
N++Y+ S M+PF+ALYGR L + DL E + +R + SQ
Sbjct: 1 NSNYNRSAKMSPFQALYGREPPVLLQGTTIPSKIAAVNDLQVGRDELLSDLRANLLKSQD 180
Query: 192 RQKSYHDKRRKALEFQEGDHVFLRVTPMTGVGHALK-SRKLTPKFIGPYQISERVGTVAY 250
++Y +K+R+ +++Q GD VFL++ P A K + KL+P++ GPY I ++G VAY
Sbjct: 181 MMRTYANKKRRDVDYQIGDEVFLKLQPYRRRSLAKKMNEKLSPRYYGPYPIVAKIGAVAY 360
Query: 251 RVGLPPHLSNLHDVFHVSQLR 271
R+ LP H S +H VFHVS L+
Sbjct: 361 RLELPAH-SRVHPVFHVSLLK 420
>BF177840
Length = 410
Score = 89.0 bits (219), Expect = 1e-18
Identities = 50/130 (38%), Positives = 73/130 (55%)
Frame = +2
Query: 58 SSIVSDRDPRFTSRFWKSLQDALGSKLRLSSAYHPQTDGQSKRTIQSLEYLLRVCVLEEG 117
+SIVSDRD +F S FW++L +G+KL S+ HPQTDGQ++ ++L LLR +
Sbjct: 11 TSIVSDRDTKFISHFWRTLWGKVGTKLLYSTTCHPQTDGQTEVVNKTLSTLLRSVLERNL 190
Query: 118 GAWDSHLPLIEFTYNNSYHSSIGMAPFKALYGRRCRTPLCWFESGESVVLGPDLVHETTE 177
W++ LP IEF YN HS+ +PF+ +YG TPL + +L E
Sbjct: 191 KMWETWLPHIEFAYNRVVHSTTKHSPFEIVYGYNPLTPLDLLPMPNTYLLKHKDGKAKAE 370
Query: 178 KVKMIRKKMK 187
VK + +K+K
Sbjct: 371 FVKRLHEKIK 400
>BP046863
Length = 580
Score = 59.7 bits (143), Expect = 7e-10
Identities = 35/103 (33%), Positives = 61/103 (58%), Gaps = 1/103 (0%)
Frame = +1
Query: 171 LVHETTEKVKMIRKKMKASQSRQKSYHDKRRKALEFQEGDHVFLRVTPMTGVGHAL-KSR 229
L+ E + +R + +Q + ++ +K R+ +++Q G+ VFL++ P A K++
Sbjct: 274 LIEERDALLLELRGNLLKAQDQMRAQANKHRRYVDYQVGNWVFLKLQPYKLQNLAQRKNQ 453
Query: 230 KLTPKFIGPYQISERVGTVAYRVGLPPHLSNLHDVFHVSQLRK 272
KL+P+F GP+++ ERV VAY + L S +H VFH+S L K
Sbjct: 454 KLSPRFYGPFKVLERVVQVAY*LDLXSE-SRVHPVFHLSLLEK 579
>TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase region
(Fragment), partial (10%)
Length = 572
Score = 55.1 bits (131), Expect = 2e-08
Identities = 29/90 (32%), Positives = 48/90 (53%)
Frame = +2
Query: 67 RFTSRFWKSLQDALGSKLRLSSAYHPQTDGQSKRTIQSLEYLLRVCVLEEGGAWDSHLPL 126
+FTS+ + D +G ++R SS HPQT+GQ++ + + ++ + E G W LP+
Sbjct: 20 QFTSKQTQDFCDGMGIQMRFSSVKHPQTNGQTEAANKVILKGIKRRLYEAEGRWIDELPI 199
Query: 127 IEFTYNNSYHSSIGMAPFKALYGRRCRTPL 156
+ ++YN SSI PF YG P+
Sbjct: 200 VLWSYNTMPQSSIKETPF*LTYGADTMLPV 289
>BP085845
Length = 464
Score = 42.7 bits (99), Expect = 8e-05
Identities = 31/106 (29%), Positives = 48/106 (45%), Gaps = 17/106 (16%)
Frame = -2
Query: 227 KSRKLTPKFIGPYQISERVGTVAYRVGLPPHLSNLHDVFHVSQLRKYVADPSHVIPRDDV 286
K RK K IGP++I E V +AY + +L H V HV+ L + + D S I + V
Sbjct: 421 KKRKTESKIIGPFKILEMVCLIAY*LTHSLYLLAAHIVLHVTLLWRNLYDQSQNICHEGV 242
Query: 287 QVRDNLTVETLPL-----------------VKVVWDGATGESLTWE 315
+ ++ + P+ VKV+W G +G +WE
Sbjct: 241 XLGEHWSHMEHPIVMVDMKVRCMRPKNIDDVKVIWRGLSG*EKSWE 104
>TC11563
Length = 470
Score = 28.9 bits (63), Expect = 1.2
Identities = 12/30 (40%), Positives = 21/30 (70%)
Frame = +2
Query: 297 LPLVKVVWDGATGESLTWELESKMRDSYPK 326
+P + + W+GA + TWEL S ++DS+P+
Sbjct: 8 VPQLLIQWEGAA--NCTWELLSYIQDSFPQ 91
>TC16411 similar to GB|AAP31965.1|30387599|BT006621 At4g21540 {Arabidopsis
thaliana;}, partial (24%)
Length = 555
Score = 26.9 bits (58), Expect = 4.7
Identities = 11/30 (36%), Positives = 14/30 (46%)
Frame = +2
Query: 149 GRRCRTPLCWFESGESVVLGPDLVHETTEK 178
GR C+ P+C GE LG H+ K
Sbjct: 119 GRTCKIPICHIPKGEGFQLGTWSTHQGQRK 208
>BP072713
Length = 403
Score = 26.6 bits (57), Expect = 6.1
Identities = 14/27 (51%), Positives = 15/27 (54%)
Frame = +3
Query: 222 VGHALKSRKLTPKFIGPYQISERVGTV 248
V LKS K P+ G Y SERVG V
Sbjct: 162 VVQTLKSLKWPPRIPGYYDYSERVGVV 242
>TC12763 similar to UP|CADH_MEDSA (P31656) Cinnamyl-alcohol dehydrogenase
(CAD) , partial (62%)
Length = 809
Score = 26.2 bits (56), Expect = 8.0
Identities = 9/46 (19%), Positives = 24/46 (51%)
Frame = -2
Query: 153 RTPLCWFESGESVVLGPDLVHETTEKVKMIRKKMKASQSRQKSYHD 198
+ LCWF ++++ PD + +++ + A+ +++ S H+
Sbjct: 268 KATLCWFSIYINIIVIPDFLFAVLLNIRLACRAFFAASNKEASSHN 131
>BP033788
Length = 518
Score = 26.2 bits (56), Expect = 8.0
Identities = 11/30 (36%), Positives = 19/30 (62%)
Frame = +3
Query: 74 KSLQDALGSKLRLSSAYHPQTDGQSKRTIQ 103
KSLQ G++++L + P+ D +RT+Q
Sbjct: 75 KSLQTKSGARIQLIPQHLPEGDDSKERTVQ 164
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.136 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,894,986
Number of Sequences: 28460
Number of extensions: 78520
Number of successful extensions: 337
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 334
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 334
length of query: 329
length of database: 4,897,600
effective HSP length: 91
effective length of query: 238
effective length of database: 2,307,740
effective search space: 549242120
effective search space used: 549242120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)
Medicago: description of AC130807.4