BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC130200.8 + phase: 0
(136 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17955 PIR|S04520|S04520 histone H3 (clone pH3c-1) - alfalfa {M... 263 6e-72
TC14950 PIR|S04520|S04520 histone H3 (clone pH3c-1) - alfalfa {M... 263 6e-72
TC19421 PIR|S04520|S04520 histone H3 (clone pH3c-1) - alfalfa {M... 261 3e-71
TC9673 UP|BAA31218 (BAA31218) Histone H3, complete 256 7e-70
TC8062 UP|BAA31218 (BAA31218) Histone H3, complete 256 7e-70
TC8063 UP|BAA31218 (BAA31218) Histone H3, complete 256 7e-70
TC16931 PIR|S04520|S04520 histone H3 (clone pH3c-1) - alfalfa {M... 176 8e-64
TC8064 homologue to GB|AAB36496.1|488573|MSU09463 histone H3.2 p... 104 6e-24
AV413508 91 9e-20
BP037032 30 0.11
TC13548 similar to GB|AAO63329.1|28950811|BT005265 At5g21920 {Ar... 25 6.1
BP071195 24 7.9
>TC17955 PIR|S04520|S04520 histone H3 (clone pH3c-1) - alfalfa {Medicago
sativa;}, complete
Length = 639
Score = 263 bits (673), Expect = 6e-72
Identities = 136/136 (100%), Positives = 136/136 (100%)
Frame = +1
Query: 1 MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTE 60
MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTE
Sbjct: 58 MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTE 237
Query: 61 LLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTI 120
LLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTI
Sbjct: 238 LLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTI 417
Query: 121 MPKDIQLARRIRGERA 136
MPKDIQLARRIRGERA
Sbjct: 418 MPKDIQLARRIRGERA 465
>TC14950 PIR|S04520|S04520 histone H3 (clone pH3c-1) - alfalfa {Medicago
sativa;}, complete
Length = 641
Score = 263 bits (673), Expect = 6e-72
Identities = 136/136 (100%), Positives = 136/136 (100%)
Frame = +2
Query: 1 MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTE 60
MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTE
Sbjct: 65 MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTE 244
Query: 61 LLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTI 120
LLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTI
Sbjct: 245 LLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTI 424
Query: 121 MPKDIQLARRIRGERA 136
MPKDIQLARRIRGERA
Sbjct: 425 MPKDIQLARRIRGERA 472
>TC19421 PIR|S04520|S04520 histone H3 (clone pH3c-1) - alfalfa {Medicago
sativa;}, complete
Length = 672
Score = 261 bits (667), Expect = 3e-71
Identities = 135/136 (99%), Positives = 135/136 (99%)
Frame = +3
Query: 1 MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTE 60
MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTE
Sbjct: 93 MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTE 272
Query: 61 LLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTI 120
LLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTI
Sbjct: 273 LLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTI 452
Query: 121 MPKDIQLARRIRGERA 136
MPKDIQLAR IRGERA
Sbjct: 453 MPKDIQLARXIRGERA 500
>TC9673 UP|BAA31218 (BAA31218) Histone H3, complete
Length = 690
Score = 256 bits (655), Expect = 7e-70
Identities = 132/136 (97%), Positives = 133/136 (97%)
Frame = +1
Query: 1 MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTE 60
MARTKQTARKSTGGKAPRKQLATKAARKSAP TGGVKKPHR+RPGTVALREIRKYQKSTE
Sbjct: 76 MARTKQTARKSTGGKAPRKQLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTE 255
Query: 61 LLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTI 120
LLIRKLPFQRLVREIAQDFKTDLRFQS AV ALQEAAEAYLVGLFEDTNLCAIHAKRVTI
Sbjct: 256 LLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTI 435
Query: 121 MPKDIQLARRIRGERA 136
MPKDIQLARRIRGERA
Sbjct: 436 MPKDIQLARRIRGERA 483
>TC8062 UP|BAA31218 (BAA31218) Histone H3, complete
Length = 768
Score = 256 bits (655), Expect = 7e-70
Identities = 132/136 (97%), Positives = 133/136 (97%)
Frame = +3
Query: 1 MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTE 60
MARTKQTARKSTGGKAPRKQLATKAARKSAP TGGVKKPHR+RPGTVALREIRKYQKSTE
Sbjct: 51 MARTKQTARKSTGGKAPRKQLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTE 230
Query: 61 LLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTI 120
LLIRKLPFQRLVREIAQDFKTDLRFQS AV ALQEAAEAYLVGLFEDTNLCAIHAKRVTI
Sbjct: 231 LLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTI 410
Query: 121 MPKDIQLARRIRGERA 136
MPKDIQLARRIRGERA
Sbjct: 411 MPKDIQLARRIRGERA 458
>TC8063 UP|BAA31218 (BAA31218) Histone H3, complete
Length = 723
Score = 256 bits (655), Expect = 7e-70
Identities = 132/136 (97%), Positives = 133/136 (97%)
Frame = +2
Query: 1 MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTE 60
MARTKQTARKSTGGKAPRKQLATKAARKSAP TGGVKKPHR+RPGTVALREIRKYQKSTE
Sbjct: 71 MARTKQTARKSTGGKAPRKQLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTE 250
Query: 61 LLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTI 120
LLIRKLPFQRLVREIAQDFKTDLRFQS AV ALQEAAEAYLVGLFEDTNLCAIHAKRVTI
Sbjct: 251 LLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTI 430
Query: 121 MPKDIQLARRIRGERA 136
MPKDIQLARRIRGERA
Sbjct: 431 MPKDIQLARRIRGERA 478
>TC16931 PIR|S04520|S04520 histone H3 (clone pH3c-1) - alfalfa {Medicago
sativa;}, partial (96%)
Length = 440
Score = 176 bits (446), Expect(2) = 8e-64
Identities = 90/90 (100%), Positives = 90/90 (100%)
Frame = +1
Query: 1 MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTE 60
MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTE
Sbjct: 46 MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTE 225
Query: 61 LLIRKLPFQRLVREIAQDFKTDLRFQSSAV 90
LLIRKLPFQRLVREIAQDFKTDLRFQSSAV
Sbjct: 226 LLIRKLPFQRLVREIAQDFKTDLRFQSSAV 315
Score = 82.4 bits (202), Expect(2) = 8e-64
Identities = 41/41 (100%), Positives = 41/41 (100%)
Frame = +2
Query: 91 SALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRI 131
SALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRI
Sbjct: 317 SALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRI 439
>TC8064 homologue to GB|AAB36496.1|488573|MSU09463 histone H3.2 precursor
{Medicago sativa;} , partial (45%)
Length = 486
Score = 104 bits (259), Expect = 6e-24
Identities = 53/57 (92%), Positives = 55/57 (95%)
Frame = +1
Query: 80 KTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA 136
+TDLRFQS AV ALQEAAEAYLVGLFEDT+LCAIHAKRVTIMPKDIQLARRIRGERA
Sbjct: 79 QTDLRFQSHAVLALQEAAEAYLVGLFEDTHLCAIHAKRVTIMPKDIQLARRIRGERA 249
>AV413508
Length = 265
Score = 90.5 bits (223), Expect = 9e-20
Identities = 52/87 (59%), Positives = 55/87 (62%), Gaps = 28/87 (32%)
Frame = +3
Query: 29 SAPATGGVKKPHRFRPGTVALR----------------------------EIRKYQKSTE 60
SAP TGGVKKPHR+RPGTVALR EIRKYQKSTE
Sbjct: 3 SAPTTGGVKKPHRYRPGTVALR*FTVFPQILSRFLSVAED*IFLFVYNFSEIRKYQKSTE 182
Query: 61 LLIRKLPFQRLVREIAQDFKTDLRFQS 87
LLIRKLPFQRLVREIAQDFK L F++
Sbjct: 183LLIRKLPFQRLVREIAQDFKVILVFKT 263
>BP037032
Length = 577
Score = 30.4 bits (67), Expect = 0.11
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
Frame = +1
Query: 23 TKAARKSAPATGGVKK-PHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVRE 74
T+ R++ P G + PHR RP V + + + LIR LP R++R+
Sbjct: 142 TQRPRRTNPPPGAERHHPHRLRPRNVRVHTVSDQKPLGSELIRILPHVRILRQ 300
>TC13548 similar to GB|AAO63329.1|28950811|BT005265 At5g21920 {Arabidopsis
thaliana;}, partial (22%)
Length = 515
Score = 24.6 bits (52), Expect = 6.1
Identities = 12/32 (37%), Positives = 17/32 (52%)
Frame = -3
Query: 15 KAPRKQLATKAARKSAPATGGVKKPHRFRPGT 46
KA R + A R S P GG+ ++FR G+
Sbjct: 129 KAFRTRNARMGERSSVPPRGGINPRNKFRYGS 34
>BP071195
Length = 424
Score = 24.3 bits (51), Expect = 7.9
Identities = 11/29 (37%), Positives = 17/29 (57%)
Frame = -3
Query: 4 TKQTARKSTGGKAPRKQLATKAARKSAPA 32
+K+ R S+ + PR+ LAT A +A A
Sbjct: 302 SKRVTRSSSSHRRPRETLATAATASTATA 216
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.132 0.359
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,438,344
Number of Sequences: 28460
Number of extensions: 12357
Number of successful extensions: 72
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 71
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 72
length of query: 136
length of database: 4,897,600
effective HSP length: 81
effective length of query: 55
effective length of database: 2,592,340
effective search space: 142578700
effective search space used: 142578700
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)
Medicago: description of AC130200.8