BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC129091.17 + phase: 0 /pseudo
(136 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19283 similar to UP|Q8H0V8 (Q8H0V8) Spliceosome associated pro... 89 4e-29
BP049935 66 4e-18
AV780938 68 8e-18
AW428654 33 0.017
CB829198 33 0.017
TC14320 similar to UP|Q41111 (Q41111) Dehydrin, partial (56%) 32 0.029
TC10380 similar to UP|Q8S8U2 (Q8S8U2) Ycf1 protein, partial (40%) 30 0.11
TC14083 similar to UP|CRTC_RICCO (P93508) Calreticulin precursor... 30 0.14
TC17544 similar to PIR|T04919|T04919 DNA-binding protein homolog... 30 0.19
TC14547 weakly similar to UP|VILI_CHICK (P02640) Villin, partial... 28 0.42
CN825379 28 0.55
TC9596 similar to UP|AAR24722 (AAR24722) At5g49400, partial (66%) 28 0.72
AW163921 27 0.94
TC14318 similar to UP|O64739 (O64739) Ran-binding protein (AtRan... 27 0.94
TC14766 similar to UP|Q7XXR8 (Q7XXR8) Nascent polypeptide associ... 27 1.2
TC12380 similar to GB|BAC55960.1|28071265|AB087408 actin binding... 27 1.6
TC8713 similar to UP|P93704 (P93704) HMG-1, partial (91%) 27 1.6
TC10598 weakly similar to GB|AAM78036.1|21928015|AY125526 At2g01... 27 1.6
AV774953 26 2.1
AU251825 26 2.1
>TC19283 similar to UP|Q8H0V8 (Q8H0V8) Spliceosome associated protein-like,
partial (21%)
Length = 526
Score = 88.6 bits (218), Expect(2) = 4e-29
Identities = 46/82 (56%), Positives = 59/82 (71%)
Frame = +3
Query: 16 EVAASEETDKKDVAEETAATKKKANSDSDYEDEENDNEQKEKGVSNKKKKLQRRMKIAEL 75
E + D D + T K+ +SDS+ ++EEND+E K+KG SNKKKKLQ+RMKIAEL
Sbjct: 45 ETLPHQNKDNDDAKDNT--DPKQGDSDSE-DEEENDDELKKKGTSNKKKKLQQRMKIAEL 215
Query: 76 KQVSSRPDVVEVWDATAADPKL 97
K + SRP VVE WDATA+DPK+
Sbjct: 216 KHICSRPYVVEAWDATASDPKV 281
Score = 53.9 bits (128), Expect(2) = 4e-29
Identities = 24/27 (88%), Positives = 24/27 (88%)
Frame = +2
Query: 97 LLVFLKSYRNTVPVPRHWSQKRKFLQG 123
LLVFLKSYRNTVPVPRHWSQKRK G
Sbjct: 281 LLVFLKSYRNTVPVPRHWSQKRKVFAG 361
>BP049935
Length = 438
Score = 66.2 bits (160), Expect(2) = 4e-18
Identities = 29/34 (85%), Positives = 31/34 (90%)
Frame = -1
Query: 87 VWDATAADPKLLVFLKSYRNTVPVPRHWSQKRKF 120
VWDATA+DPKLL FLKSY NTVP+PRHWSQKR F
Sbjct: 300 VWDATASDPKLLGFLKSYSNTVPMPRHWSQKRVF 199
Score = 39.3 bits (90), Expect(2) = 4e-18
Identities = 14/20 (70%), Positives = 18/20 (90%)
Frame = -2
Query: 117 KRKFLQGSGCGNHQSWTGRC 136
+R+FLQG GCGN+Q+WT RC
Sbjct: 212 RREFLQGRGCGNYQAWTCRC 153
>AV780938
Length = 536
Score = 68.2 bits (165), Expect(2) = 8e-18
Identities = 43/108 (39%), Positives = 53/108 (48%), Gaps = 35/108 (32%)
Frame = -3
Query: 48 EENDNEQKEKGVSNKKK------------------------KLQRRMKI----------- 72
EENDNE ++KG+S+K K R +
Sbjct: 534 EENDNELEKKGISHKNKL*LYVTCIFYFCCLSIFYCRLPFENCTERFNLVSASFY*FGIM 355
Query: 73 AELKQVSSRPDVVEVWDATAADPKLLVFLKSYRNTVPVPRHWSQKRKF 120
A L + S + +VWDATA+DPKLL FLKSY TVP+PRHWSQKR F
Sbjct: 354 AILDMLGSFFFIFQVWDATASDPKLLGFLKSYSYTVPMPRHWSQKRVF 211
Score = 36.2 bits (82), Expect(2) = 8e-18
Identities = 13/20 (65%), Positives = 17/20 (85%)
Frame = -1
Query: 117 KRKFLQGSGCGNHQSWTGRC 136
+R+FLQG GCG +Q+WT RC
Sbjct: 224 RREFLQGRGCGYYQAWTCRC 165
>AW428654
Length = 373
Score = 33.1 bits (74), Expect = 0.017
Identities = 18/60 (30%), Positives = 29/60 (48%), Gaps = 1/60 (1%)
Frame = +3
Query: 11 KFSFTEVAASEETDKKDVAEETAATKKKANSDSDYEDEENDNE-QKEKGVSNKKKKLQRR 69
K+ F + E T + E + + + S S+YEDEEN N+ Q + + +K RR
Sbjct: 81 KYEFLDATEGEHTPRTPFPEFLSTPQDQEESSSEYEDEENANDPQTVQPTATSTRKRGRR 260
>CB829198
Length = 562
Score = 33.1 bits (74), Expect = 0.017
Identities = 16/61 (26%), Positives = 31/61 (50%)
Frame = +3
Query: 21 EETDKKDVAEETAATKKKANSDSDYEDEENDNEQKEKGVSNKKKKLQRRMKIAELKQVSS 80
EE DV E + K+ + + ED +N+N + ++ + KKKK + R ++ + V
Sbjct: 255 EEPQNDDVEESQHSDSKEETEEEEDEDGDNENNKPKRAKAAKKKKNKERSNKSDNEVVKK 434
Query: 81 R 81
+
Sbjct: 435 K 437
>TC14320 similar to UP|Q41111 (Q41111) Dehydrin, partial (56%)
Length = 1231
Score = 32.3 bits (72), Expect = 0.029
Identities = 31/107 (28%), Positives = 45/107 (41%), Gaps = 1/107 (0%)
Frame = +3
Query: 4 EFRKIFEKFSFTEVAASEETDKKDVAEETAATKKKANSDSDYEDEENDNEQKEKGVSNKK 63
EF K+ K S EE D + + + +K SDS ++ E KE KK
Sbjct: 486 EFEKV--KVSEEPEKKKEEVDHEGEKKHSTLLEKLHRSDSSSSSSSDEEEGKEGEEKKKK 659
Query: 64 KKLQRRMKIAELKQVSSRPDV-VEVWDATAADPKLLVFLKSYRNTVP 109
KK +++ E+K V VEV A A + K FL + +P
Sbjct: 660 KKKEKK----EVKSTDENTSVPVEVDPAHAEEKK--GFLDKIKEKLP 782
>TC10380 similar to UP|Q8S8U2 (Q8S8U2) Ycf1 protein, partial (40%)
Length = 5664
Score = 30.4 bits (67), Expect = 0.11
Identities = 16/41 (39%), Positives = 25/41 (60%), Gaps = 2/41 (4%)
Frame = +2
Query: 36 KKKANSDSDYEDEENDNEQK--EKGVSNKKKKLQRRMKIAE 74
KKK +SDY DE+ QK E+ + +K+ +RR++I E
Sbjct: 2195 KKKELKNSDYTDEKAKESQKKEEEKIKKNEKEEKRRIEIGE 2317
>TC14083 similar to UP|CRTC_RICCO (P93508) Calreticulin precursor, partial
(92%)
Length = 1693
Score = 30.0 bits (66), Expect = 0.14
Identities = 18/38 (47%), Positives = 24/38 (62%)
Frame = +1
Query: 16 EVAASEETDKKDVAEETAATKKKANSDSDYEDEENDNE 53
E AA EE +KK EE+ K+ +DSD EDEE+ +E
Sbjct: 1180 EKAAFEEAEKKREEEES----KEDPADSDAEDEEDTDE 1281
>TC17544 similar to PIR|T04919|T04919 DNA-binding protein homolog T9A21.10 -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(14%)
Length = 578
Score = 29.6 bits (65), Expect = 0.19
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = +1
Query: 15 TEVAASEETDKKDVAEETAATKKKANSDSDYEDEENDNEQKEKGVSNKKK 64
+ ++AS +D AEE + KKK N+ E + N+ K+KG +K+
Sbjct: 367 SSISASSSSDAG--AEEDSGKKKKDNNQVKEEGNNSGNKDKKKGEKKQKE 510
>TC14547 weakly similar to UP|VILI_CHICK (P02640) Villin, partial (5%)
Length = 794
Score = 28.5 bits (62), Expect = 0.42
Identities = 20/68 (29%), Positives = 37/68 (54%), Gaps = 5/68 (7%)
Frame = +2
Query: 15 TEVAASEETDKKDVAE-ETAATKKKANSDSDYEDE----ENDNEQKEKGVSNKKKKLQRR 69
+E A SEE +++ E E A + +N DS+ + E END++ ++ S +K K +
Sbjct: 17 SESAHSEEALEEEXKEREELAPETGSNGDSEPKQENVEDENDSQNSQRFFSYEKLKAKSE 196
Query: 70 MKIAELKQ 77
+ +LK+
Sbjct: 197LSGVDLKR 220
>CN825379
Length = 646
Score = 28.1 bits (61), Expect = 0.55
Identities = 17/62 (27%), Positives = 30/62 (47%)
Frame = +3
Query: 21 EETDKKDVAEETAATKKKANSDSDYEDEENDNEQKEKGVSNKKKKLQRRMKIAELKQVSS 80
+E K++ E K+K +YEDEE + +E+ +KKK QR ++ ++
Sbjct: 162 DEAPKQESLEAKQQRKRKRMKLQEYEDEEAEVVVEEE--DEEKKKNQRNPPTSDAEEEQD 335
Query: 81 RP 82
P
Sbjct: 336 AP 341
>TC9596 similar to UP|AAR24722 (AAR24722) At5g49400, partial (66%)
Length = 1196
Score = 27.7 bits (60), Expect = 0.72
Identities = 13/41 (31%), Positives = 22/41 (52%)
Frame = +2
Query: 39 ANSDSDYEDEENDNEQKEKGVSNKKKKLQRRMKIAELKQVS 79
++SDS D E + ++ K + KKKK +R K + + S
Sbjct: 734 SSSDSSSSDSEEERRRRRKKKTQKKKKGRRHKKYSSSSESS 856
>AW163921
Length = 450
Score = 27.3 bits (59), Expect = 0.94
Identities = 22/62 (35%), Positives = 29/62 (46%), Gaps = 6/62 (9%)
Frame = -3
Query: 2 DEEFRKIFEKFSFTEVAASEETDKKDV------AEETAATKKKANSDSDYEDEENDNEQK 55
D EF + FE FSF + D++D AE AAT K N+ S +EN +Q
Sbjct: 334 DIEFCQNFE-FSFKLLIVDRRKDQEDTTAVINGAEGLAATT*KVNNRSSQSKQENWAQQS 158
Query: 56 EK 57
K
Sbjct: 157 GK 152
>TC14318 similar to UP|O64739 (O64739) Ran-binding protein (AtRanBP1b),
partial (73%)
Length = 1099
Score = 27.3 bits (59), Expect = 0.94
Identities = 15/46 (32%), Positives = 26/46 (55%), Gaps = 4/46 (8%)
Frame = +1
Query: 8 IFEKFSFTEVAASEETDK----KDVAEETAATKKKANSDSDYEDEE 49
+ EK S E A +E+ D+ E+ A+ +K+ +D+D +DEE
Sbjct: 583 LIEKLSVEEKADAEKKDEVKTESKAEEQEPASGEKSKADADKKDEE 720
>TC14766 similar to UP|Q7XXR8 (Q7XXR8) Nascent polypeptide associated
complex alpha chain, partial (58%)
Length = 600
Score = 26.9 bits (58), Expect = 1.2
Identities = 23/89 (25%), Positives = 42/89 (46%), Gaps = 2/89 (2%)
Frame = +2
Query: 17 VAASEETDKKDVAEETAATKKKANSDSDYEDEENDNEQKEKGVSNKKKKLQRRMKIAE-- 74
+AA E K E + + D D +DE++DN + ++G ++ + K R K +
Sbjct: 86 LAAHLEQQKIHDDEPVVEDDDEDDDDEDDDDEDDDNIEGQEGDASGRSKQTRSEKKSRKA 265
Query: 75 LKQVSSRPDVVEVWDATAADPKLLVFLKS 103
+ ++ +P V V T K +VF+ S
Sbjct: 266 MLKLGMKP-VTGVSRVTVKKSKNIVFVIS 349
>TC12380 similar to GB|BAC55960.1|28071265|AB087408 actin binding protein
{Arabidopsis thaliana;} , partial (9%)
Length = 800
Score = 26.6 bits (57), Expect = 1.6
Identities = 13/47 (27%), Positives = 25/47 (52%)
Frame = +1
Query: 2 DEEFRKIFEKFSFTEVAASEETDKKDVAEETAATKKKANSDSDYEDE 48
DEE + + SFT+ E++++++ EE N +D+ED+
Sbjct: 442 DEEGTEEEQFKSFTDAINEEDSEEEEEKEEVKLISSIINRANDFEDD 582
>TC8713 similar to UP|P93704 (P93704) HMG-1, partial (91%)
Length = 800
Score = 26.6 bits (57), Expect = 1.6
Identities = 11/30 (36%), Positives = 18/30 (59%)
Frame = +2
Query: 24 DKKDVAEETAATKKKANSDSDYEDEENDNE 53
+ K AEE + K K+ + D ED++ D+E
Sbjct: 461 ESKGSAEEEGSDKSKSEVNDDDEDDDEDDE 550
>TC10598 weakly similar to GB|AAM78036.1|21928015|AY125526
At2g01100/F23H14.7 {Arabidopsis thaliana;}, partial
(33%)
Length = 902
Score = 26.6 bits (57), Expect = 1.6
Identities = 13/53 (24%), Positives = 28/53 (52%)
Frame = +3
Query: 35 TKKKANSDSDYEDEENDNEQKEKGVSNKKKKLQRRMKIAELKQVSSRPDVVEV 87
TKK ++ SD+ +E+++ +E+ +K+K + R +++ S EV
Sbjct: 540 TKKWSSESSDFSGDESESRSEEERRRKRKQKKKLRDQVSRSDSSDSDSSADEV 698
>AV774953
Length = 579
Score = 26.2 bits (56), Expect = 2.1
Identities = 19/66 (28%), Positives = 29/66 (43%), Gaps = 1/66 (1%)
Frame = +1
Query: 10 EKFSFTEVAASEETDKKDVAEETAATKKKANSDSDYEDEENDNEQKE-KGVSNKKKKLQR 68
EK + +ASEE + AEE A A+ + + EE E V+ KK + +
Sbjct: 343 EKDNTETESASEEGKAESAAEENTADPNDADDKPETDAEETKQSNVEGDNVTAKKPQGEE 522
Query: 69 RMKIAE 74
+AE
Sbjct: 523 EASLAE 540
>AU251825
Length = 237
Score = 26.2 bits (56), Expect = 2.1
Identities = 15/53 (28%), Positives = 24/53 (44%)
Frame = +3
Query: 24 DKKDVAEETAATKKKANSDSDYEDEENDNEQKEKGVSNKKKKLQRRMKIAELK 76
DKK ++ AA ++KA + E KE G + KKK + + + K
Sbjct: 24 DKKPAEKKPAAAEEKAAAAEKKPRAEKKIPAKEGGAAGDKKKKRSKKNVETYK 182
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.309 0.125 0.357
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,065,778
Number of Sequences: 28460
Number of extensions: 23999
Number of successful extensions: 192
Number of sequences better than 10.0: 64
Number of HSP's better than 10.0 without gapping: 185
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 187
length of query: 136
length of database: 4,897,600
effective HSP length: 81
effective length of query: 55
effective length of database: 2,592,340
effective search space: 142578700
effective search space used: 142578700
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 50 (23.9 bits)
Medicago: description of AC129091.17