BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC129091.14 + phase: 0
(439 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CB827391 39 3e-06
TC18123 39 0.002
TC14645 similar to UP|Q41122 (Q41122) Proline-rich protein precu... 35 0.032
AU251535 32 0.16
BP043430 32 0.21
BP039004 32 0.27
BP041257 31 0.46
TC14287 similar to UP|Q9LEU7 (Q9LEU7) Serine/threonine protein k... 31 0.46
AW164001 31 0.46
TC13861 weakly similar to UP|Q9S4J5 (Q9S4J5) Serum opacity facto... 30 0.60
BP049555 30 0.60
TC17759 similar to UP|Q944Q8 (Q944Q8) AT5g46340/MPL12_14, partia... 30 0.79
BP039352 30 1.0
TC16259 weakly similar to UP|O23300 (O23300) Carnitine racemase ... 30 1.0
AV412404 29 1.3
BP056337 29 1.3
AV414962 29 1.3
BP038135 29 1.3
TC17867 similar to GB|AAM26654.1|20856224|AY101533 At1g14810/F10... 29 1.3
BP055520 29 1.3
>CB827391
Length = 519
Score = 38.9 bits (89), Expect(4) = 3e-06
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +1
Query: 335 GNKWGHNGPYLISRVVSRVSGREGYNFSVVP 365
G + G+NGPYL SR V ++ R +NF ++P
Sbjct: 31 GKQMGYNGPYLFSRAVGKLGNRPDFNFKILP 123
Score = 22.7 bits (47), Expect(4) = 3e-06
Identities = 7/14 (50%), Positives = 10/14 (71%)
Frame = +3
Query: 366 PSAFYPVDWRGIKS 379
P +FYP DW+ + S
Sbjct: 123 PISFYPEDWKNLGS 164
Score = 21.9 bits (45), Expect(4) = 3e-06
Identities = 8/27 (29%), Positives = 16/27 (58%)
Frame = +2
Query: 412 RQSGKLEVVKGSIIDSIISSCCIFCNT 438
RQS + +GS++ ++ C+ CN+
Sbjct: 257 RQSSG*MMGEGSVLARLVLDHCVICNS 337
Score = 21.2 bits (43), Expect(4) = 3e-06
Identities = 6/9 (66%), Positives = 8/9 (88%)
Frame = +2
Query: 331 LTFDGNKWG 339
+ F+GNKWG
Sbjct: 20 IEFEGNKWG 46
>TC18123
Length = 656
Score = 38.5 bits (88), Expect = 0.002
Identities = 24/101 (23%), Positives = 42/101 (40%), Gaps = 5/101 (4%)
Frame = +2
Query: 341 NGPYLISRVVSRVSGREG-----YNFSVVPPSAFYPVDWRGIKSLFRGPGDEIHSKWLVK 395
+G L++RV + G + + V P F+P+ + I F P E
Sbjct: 14 DGADLLTRVARKFMGDDNKSIKQLDLKVEPSHIFFPITSQNIARYFIAPATETEKAQEDV 193
Query: 396 KMVQIRKESYAVHLWNRQSGKLEVVKGSIIDSIISSCCIFC 436
+ +I +ES H WN + L S++ +++ CI C
Sbjct: 194 LLKKIMQESLTFHFWNSLTSALIPEPDSLVTRLMNYACIRC 316
>TC14645 similar to UP|Q41122 (Q41122) Proline-rich protein precursor,
partial (52%)
Length = 1060
Score = 34.7 bits (78), Expect = 0.032
Identities = 23/79 (29%), Positives = 33/79 (41%), Gaps = 14/79 (17%)
Frame = +2
Query: 49 LVLFLLAYNAFTVFC--------------IHIPHHYSPHKPLLLPPPSHTKTKLSSSVFF 94
L LFLL +FTVF + +PH+ H P+ PPP++ ++
Sbjct: 68 LALFLLLATSFTVFAQDQEVLNPSTIPSLVALPHNVPLHPPVAAPPPNYHNHPVAP---- 235
Query: 95 AHKEDNSPIINKTHFPLLH 113
H +SPI H P H
Sbjct: 236 VHPPTHSPIHPPAHAPHHH 292
>AU251535
Length = 359
Score = 32.3 bits (72), Expect = 0.16
Identities = 18/44 (40%), Positives = 22/44 (49%)
Frame = +3
Query: 39 FKLPTSFFALLVLFLLAYNAFTVFCIHIPHHYSPHKPLLLPPPS 82
F LPT ++ L+ F N FT I+ H SP KP PP S
Sbjct: 78 FLLPTHKWSFLLPFYFPINHFTHHPIYTTHFESPQKPDTPPPKS 209
>BP043430
Length = 493
Score = 32.0 bits (71), Expect = 0.21
Identities = 17/31 (54%), Positives = 22/31 (70%), Gaps = 2/31 (6%)
Frame = +1
Query: 1 MEDHTKHHHNPFLYFPLL-LLHKLH-HFLHN 29
+ H+ HHH+ Y PLL LLH+LH HFLH+
Sbjct: 262 LHSHSHHHHH--YYPPLLHLLHQLHYHFLHH 348
>BP039004
Length = 408
Score = 31.6 bits (70), Expect = 0.27
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = +1
Query: 65 HIPHHYSPHKPLLLPPPSHTK 85
H HHYS H P PPP H++
Sbjct: 187 HHHHHYSQHLPASTPPPEHSR 249
>BP041257
Length = 478
Score = 30.8 bits (68), Expect = 0.46
Identities = 23/78 (29%), Positives = 31/78 (39%), Gaps = 3/78 (3%)
Frame = -3
Query: 7 HHHNPFLYFPLLLLHKLHHFLHNFKKKILSILFKLPTSFFALLVLFLLAYNAFTVFCIHI 66
H H F+Y +LL H H L +F LV +L+ N T+F +H
Sbjct: 251 HLHCNFIYKTVLLSHLRLHSL----------------TFLLTLVFSILSKNHTTLFSLHP 120
Query: 67 PHHYSPHKPLLL---PPP 81
P H H + PPP
Sbjct: 119 PPHSLTHSDTTIFTHPPP 66
>TC14287 similar to UP|Q9LEU7 (Q9LEU7) Serine/threonine protein kinase-like
protein (CBL-interacting protein kinase 5), partial
(75%)
Length = 1999
Score = 30.8 bits (68), Expect = 0.46
Identities = 11/15 (73%), Positives = 12/15 (79%)
Frame = +1
Query: 67 PHHYSPHKPLLLPPP 81
PHH+ H PLLLPPP
Sbjct: 343 PHHHHHHLPLLLPPP 387
>AW164001
Length = 447
Score = 30.8 bits (68), Expect = 0.46
Identities = 13/23 (56%), Positives = 14/23 (60%)
Frame = -3
Query: 4 HTKHHHNPFLYFPLLLLHKLHHF 26
H HH NP L+ PL LH HHF
Sbjct: 106 HHHHHQNPHLHLPLCKLH--HHF 44
>TC13861 weakly similar to UP|Q9S4J5 (Q9S4J5) Serum opacity factor precursor
(Fragment), partial (9%)
Length = 632
Score = 30.4 bits (67), Expect = 0.60
Identities = 14/30 (46%), Positives = 17/30 (56%)
Frame = -2
Query: 4 HTKHHHNPFLYFPLLLLHKLHHFLHNFKKK 33
HTK +H L LLL H LHH H+ K+
Sbjct: 262 HTKRYHPKPLLTQLLLQHMLHHHHHHNNKR 173
>BP049555
Length = 555
Score = 30.4 bits (67), Expect = 0.60
Identities = 23/75 (30%), Positives = 36/75 (47%), Gaps = 16/75 (21%)
Frame = -2
Query: 53 LLAYNAF-TVFCIHIPHHYSPHK--------------PLL-LPPPSHTKTKLSSSVFFAH 96
LL+++++ + F +HIPH+ SPHK PLL PPPS + + +F
Sbjct: 470 LLSFSSYISQFSLHIPHNASPHKEHMELVV*ALLPSSPLLAFPPPSFPQLQAFPLLF--- 300
Query: 97 KEDNSPIINKTHFPL 111
P++ FPL
Sbjct: 299 -----PLLFPLSFPL 270
>TC17759 similar to UP|Q944Q8 (Q944Q8) AT5g46340/MPL12_14, partial (37%)
Length = 703
Score = 30.0 bits (66), Expect = 0.79
Identities = 22/69 (31%), Positives = 28/69 (39%)
Frame = +3
Query: 50 VLFLLAYNAFTVFCIHIPHHYSPHKPLLLPPPSHTKTKLSSSVFFAHKEDNSPIINKTHF 109
V FLL VF I Y +K PP H+ T L A KED+ I+ +T
Sbjct: 141 VSFLLG--VIPVFITWIYSEYLEYKKTAFPPKVHSDTSLDELGKDAIKEDDRAILLETGL 314
Query: 110 PLLHKISIH 118
P +H
Sbjct: 315 PRSASAKLH 341
>BP039352
Length = 528
Score = 29.6 bits (65), Expect = 1.0
Identities = 25/78 (32%), Positives = 36/78 (46%), Gaps = 13/78 (16%)
Frame = +3
Query: 17 LLLLHKLHHFLH------------NFKKKILSILFKLPTSFFALLVLFLLAY-NAFTVFC 63
L LLH LHHF +F +L +LF PT+ + +LFL+ + F +FC
Sbjct: 288 LTLLH-LHHFCFFCSSFPFSCFGLSFPFFLLFVLFCTPTTSSIITLLFLITFLLLFGLFC 464
Query: 64 IHIPHHYSPHKPLLLPPP 81
+ + H LLP P
Sbjct: 465 FLLLLWLTLHYHWLLPSP 518
>TC16259 weakly similar to UP|O23300 (O23300) Carnitine racemase like
protein (At4g14440), partial (75%)
Length = 910
Score = 29.6 bits (65), Expect = 1.0
Identities = 21/66 (31%), Positives = 32/66 (47%)
Frame = -3
Query: 63 CIHIPHHYSPHKPLLLPPPSHTKTKLSSSVFFAHKEDNSPIINKTHFPLLHKISIHKFKR 122
CIH PH P P LLP P +L ++ + N+P +++ P LH HK
Sbjct: 641 CIHFPHPLPP--PKLLPQPHRRLHRLLRAIV---RRVNNPPLHRLPRPDLHPAQ-HKVS- 483
Query: 123 VKKHKR 128
++H+R
Sbjct: 482 -QRHRR 468
>AV412404
Length = 386
Score = 29.3 bits (64), Expect = 1.3
Identities = 16/40 (40%), Positives = 19/40 (47%)
Frame = +1
Query: 42 PTSFFALLVLFLLAYNAFTVFCIHIPHHYSPHKPLLLPPP 81
P SFF L LFL ++ HH+ P PLL P P
Sbjct: 55 PLSFFILFFLFLSFHSQI--------HHHHPFPPLLPPQP 150
>BP056337
Length = 550
Score = 29.3 bits (64), Expect = 1.3
Identities = 19/55 (34%), Positives = 25/55 (44%), Gaps = 5/55 (9%)
Frame = +2
Query: 6 KHHHNPFLYFPLLLLHKL-----HHFLHNFKKKILSILFKLPTSFFALLVLFLLA 55
+HHH L LLLH L HH L + +L L L + L +FLL+
Sbjct: 383 QHHHLQSLIL*TLLLHSLLLLLPHHLLLHTLHLLLLTLHPLAQTLIIFLTIFLLS 547
>AV414962
Length = 380
Score = 29.3 bits (64), Expect = 1.3
Identities = 14/35 (40%), Positives = 20/35 (57%), Gaps = 5/35 (14%)
Frame = -2
Query: 65 HIPHHYSPHKPLLLPPPSHTK-----TKLSSSVFF 94
H+PH P P+ PPPS ++ + SSS+FF
Sbjct: 355 HLPHPNQPPPPVSPPPPSSSRFLISLSHFSSSLFF 251
>BP038135
Length = 561
Score = 29.3 bits (64), Expect = 1.3
Identities = 24/61 (39%), Positives = 32/61 (52%), Gaps = 7/61 (11%)
Frame = +1
Query: 96 HKEDNSPIINKTHFPLLHKISIHKFK-RVKKHKRGLK------TLHSDTKFPLFQKRLGA 148
H+E+++ I NK+ F KIS K K RV HK L+ T+HS FPL L +
Sbjct: 58 HEENSTQIKNKSKFHFPEKISYXKEKSRVPNHKFSLQFSFPPNTIHS-FPFPLLLLFLHS 234
Query: 149 F 149
F
Sbjct: 235 F 237
>TC17867 similar to GB|AAM26654.1|20856224|AY101533 At1g14810/F10B6_6
{Arabidopsis thaliana;}, partial (13%)
Length = 331
Score = 29.3 bits (64), Expect = 1.3
Identities = 22/77 (28%), Positives = 35/77 (44%), Gaps = 3/77 (3%)
Frame = +3
Query: 70 YSPHKPLLLPPPSHTKTKLSSSVFFAHKEDNSPIINKTHFPLLHKISIHKFKRVKKHKR- 128
++P PLLL PP+ T+T++ H + N P K +H+ +R + +R
Sbjct: 75 FAPPSPLLLRPPNPTQTQIRGG---PH*DPNVP---------SRKRPLHRHRRRHRRRRP 218
Query: 129 --GLKTLHSDTKFPLFQ 143
L+TL PL Q
Sbjct: 219 RVPLRTLRPRLPLPLHQ 269
>BP055520
Length = 397
Score = 29.3 bits (64), Expect = 1.3
Identities = 14/31 (45%), Positives = 18/31 (57%), Gaps = 3/31 (9%)
Frame = +1
Query: 3 DHTKHHHNPFLYFPLLLLHK---LHHFLHNF 30
DH HHH+ FL+FP L + HH L+ F
Sbjct: 133 DH--HHHHSFLHFPFFFLQRF*IFHHPLYYF 219
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.326 0.141 0.442
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,227,066
Number of Sequences: 28460
Number of extensions: 182386
Number of successful extensions: 1989
Number of sequences better than 10.0: 82
Number of HSP's better than 10.0 without gapping: 1902
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1969
length of query: 439
length of database: 4,897,600
effective HSP length: 93
effective length of query: 346
effective length of database: 2,250,820
effective search space: 778783720
effective search space used: 778783720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 57 (26.6 bits)
Medicago: description of AC129091.14