BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC129091.12 + phase: 0
(162 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14359 similar to UP|AAQ88099 (AAQ88099) NADPH-dependent cinnam... 88 8e-19
BP032766 86 2e-18
TC16167 similar to UP|Q9FGH3 (Q9FGH3) Dihydroflavonol 4-reductas... 86 2e-18
TC19759 similar to UP|Q8L9G4 (Q8L9G4) Cinnamyl-alcohol dehydroge... 85 5e-18
TC10957 similar to UP|Q8L9G4 (Q8L9G4) Cinnamyl-alcohol dehydroge... 83 3e-17
TC9125 similar to UP|Q40316 (Q40316) Vestitone reductase, complete 82 6e-17
TC8989 weakly similar to UP|Q9FGH3 (Q9FGH3) Dihydroflavonol 4-re... 80 1e-16
TC14360 similar to UP|O04391 (O04391) Cinnamyl alcohol dehydroge... 80 2e-16
TC15336 78 6e-16
TC13317 similar to UP|Q9SPJ5 (Q9SPJ5) Dihydroflavonol-4-reductas... 75 4e-15
TC10850 homologue to UP|Q9S6Y4 (Q9S6Y4) Dihydroflavanol reductas... 49 1e-14
TC16256 similar to UP|Q7Y0H8 (Q7Y0H8) Cinnamoyl CoA reductase, p... 71 8e-14
TC19621 similar to UP|Q8VZH7 (Q8VZH7) Cinnamoyl-CoA reductase ,... 69 3e-13
TC7966 similar to UP|Q84V25 (Q84V25) Quinone oxidoreductase, par... 69 5e-13
AV774784 49 2e-09
TC8549 weakly similar to UP|Q8LEH3 (Q8LEH3) Cinnamoyl-CoA reduct... 48 7e-07
TC13250 weakly similar to UP|Q7NJT5 (Q7NJT5) Glr1747 protein, pa... 45 8e-06
AV428109 42 5e-05
BP035620 41 1e-04
TC17328 weakly similar to UP|Q9FGH3 (Q9FGH3) Dihydroflavonol 4-r... 38 7e-04
>TC14359 similar to UP|AAQ88099 (AAQ88099) NADPH-dependent cinnamyl alcohol
dehydrogenase, partial (98%)
Length = 1403
Score = 87.8 bits (216), Expect = 8e-19
Identities = 56/132 (42%), Positives = 73/132 (54%), Gaps = 1/132 (0%)
Frame = +1
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTG +GYI S +VK LL +GYTV AT+R+ D KV L A RL LF+A++ +
Sbjct: 73 VCVTGASGYIASWIVKFLLHRGYTVKATVRDPNDSKKVDHLLNLDGAKERLHLFKANLLE 252
Query: 67 PDDFGTAIQGCEFVFHVATPYLHQT-DSQVNIQILIISLFFTHGNGAMWGRVLPSPSPSP 125
F + +QGC VFH A+P+ H D QV + + G + VL S SP
Sbjct: 253 EGSFDSVVQGCHGVFHTASPFYHDVKDPQVELLDPAV-------KGTL--NVLKSCVNSP 405
Query: 126 TLIYLPLTLSIA 137
TL + LT SIA
Sbjct: 406 TLKRVVLTSSIA 441
>BP032766
Length = 535
Score = 86.3 bits (212), Expect = 2e-18
Identities = 43/80 (53%), Positives = 56/80 (69%)
Frame = +1
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTG AG+IGS LV +L+E+GYTV AT+R+ + KV L P A T+L L++AD+ +
Sbjct: 76 VCVTGAAGFIGSWLVMRLIERGYTVRATVRDPANMKKVKHLLELPDAKTKLSLWKADLAE 255
Query: 67 PDDFGTAIQGCEFVFHVATP 86
F AI+GC VFHVATP
Sbjct: 256 EGSFDEAIRGCTGVFHVATP 315
>TC16167 similar to UP|Q9FGH3 (Q9FGH3) Dihydroflavonol 4-reductase-like
(Cinnamoyl-CoA reductase-like protein), partial (44%)
Length = 535
Score = 86.3 bits (212), Expect = 2e-18
Identities = 42/80 (52%), Positives = 56/80 (69%)
Frame = +2
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTGG+G IGS LV LL++GYTVHAT++NL DE++ L+ A+TRL LF+ D+
Sbjct: 104 VCVTGGSGCIGSWLVHLLLDRGYTVHATVQNLNDETETKHLQSLEGASTRLRLFQIDLLH 283
Query: 67 PDDFGTAIQGCEFVFHVATP 86
D A+ GC VFH+A+P
Sbjct: 284 YDTVLAAVNGCAGVFHLASP 343
>TC19759 similar to UP|Q8L9G4 (Q8L9G4) Cinnamyl-alcohol dehydrogenase-like
protein, partial (46%)
Length = 468
Score = 85.1 bits (209), Expect = 5e-18
Identities = 44/82 (53%), Positives = 55/82 (66%)
Frame = +3
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTG +GYI S +VK LLE GYTV AT+R+L + +KV L A RL LF+AD+ +
Sbjct: 18 VCVTGASGYIASWIVKFLLEHGYTVRATVRDLSNPNKVEHLVKLDVAKERLQLFKADLLE 197
Query: 67 PDDFGTAIQGCEFVFHVATPYL 88
F + IQGC VFHVA+P L
Sbjct: 198EGSFDSVIQGCHGVFHVASPVL 263
>TC10957 similar to UP|Q8L9G4 (Q8L9G4) Cinnamyl-alcohol dehydrogenase-like
protein, partial (52%)
Length = 765
Score = 82.8 bits (203), Expect = 3e-17
Identities = 42/80 (52%), Positives = 54/80 (67%)
Frame = +1
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTGG+GYI S +VK LLE GYTV AT+R+ + KV L A RL LF+AD+ +
Sbjct: 238 VCVTGGSGYIASWIVKFLLENGYTVRATVRDPGNHYKVEHLVKLDGAKERLQLFKADLLE 417
Query: 67 PDDFGTAIQGCEFVFHVATP 86
F +AI+GC VFH+A+P
Sbjct: 418 EGSFDSAIEGCNGVFHIASP 477
>TC9125 similar to UP|Q40316 (Q40316) Vestitone reductase, complete
Length = 1200
Score = 81.6 bits (200), Expect = 6e-17
Identities = 40/87 (45%), Positives = 59/87 (66%), Gaps = 2/87 (2%)
Frame = +3
Query: 2 ERRCKVCVTGGAGYIGSLLVKKLLEKGYTVHATLR-NLKDESKVSFLRGFPHANT-RLVL 59
E + +VCVTGG G++ S ++K+LLE GY+V+ T+R N + VSFL P A + +L +
Sbjct: 45 EGKGRVCVTGGTGFLASWIIKRLLEDGYSVNTTIRSNSGGKRDVSFLTNLPGATSEKLQI 224
Query: 60 FEADIYKPDDFGTAIQGCEFVFHVATP 86
F AD+ P+ FG A++GC +FH ATP
Sbjct: 225 FNADLCIPESFGPAVEGCVGIFHTATP 305
>TC8989 weakly similar to UP|Q9FGH3 (Q9FGH3) Dihydroflavonol
4-reductase-like (Cinnamoyl-CoA reductase-like protein),
partial (77%)
Length = 880
Score = 80.5 bits (197), Expect = 1e-16
Identities = 38/80 (47%), Positives = 52/80 (64%)
Frame = +2
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
+CVTG +G IGS +VK LL++GYTVHAT+++LKDE + L A RL LFE D+
Sbjct: 101 LCVTGASGAIGSWVVKLLLDRGYTVHATVQDLKDEKETKHLEAMEGAKGRLRLFEMDLLD 280
Query: 67 PDDFGTAIQGCEFVFHVATP 86
+ T ++GC V H+A P
Sbjct: 281 TNSIATTVKGCNGVIHLACP 340
>TC14360 similar to UP|O04391 (O04391) Cinnamyl alcohol dehydrogenase ,
partial (68%)
Length = 821
Score = 80.1 bits (196), Expect = 2e-16
Identities = 51/131 (38%), Positives = 71/131 (53%)
Frame = +3
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTG +GYI S +VK LL++G TV AT+R+ D KV L A RL LF+A++ +
Sbjct: 78 VCVTGASGYIASWIVKFLLQRGNTVKATVRDPNDPKKVGHLLRLDGARERLHLFKANLLE 257
Query: 67 PDDFGTAIQGCEFVFHVATPYLHQTDSQVNIQILIISLFFTHGNGAMWGRVLPSPSPSPT 126
F + +QGC VFH A+P+ + + L G + VL S + SP+
Sbjct: 258 EGSFNSVVQGCHGVFHTASPFYYDVEDPQ------AELLDPAVKGTL--NVLKSCANSPS 413
Query: 127 LIYLPLTLSIA 137
L + LT SIA
Sbjct: 414 LKRVVLTSSIA 446
>TC15336
Length = 1145
Score = 78.2 bits (191), Expect = 6e-16
Identities = 43/95 (45%), Positives = 55/95 (57%)
Frame = +2
Query: 8 CVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYKP 67
CVTGG G+I + LV+ LLEKG+TV T+RN D KV FL A RL + +AD+
Sbjct: 68 CVTGGTGFIAAYLVQALLEKGHTVRTTVRNPGDVEKVGFLMELSGAKERLKILKADLLNE 247
Query: 68 DDFGTAIQGCEFVFHVATPYLHQTDSQVNIQILII 102
F A+ G + VFH A+P L D NIQ +I
Sbjct: 248 GSFDEAVSGVDGVFHTASPVLVPYDD--NIQATLI 346
>TC13317 similar to UP|Q9SPJ5 (Q9SPJ5) Dihydroflavonol-4-reductase DFR1,
partial (36%)
Length = 426
Score = 75.5 bits (184), Expect = 4e-15
Identities = 39/81 (48%), Positives = 51/81 (62%), Gaps = 1/81 (1%)
Frame = +2
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDE-SKVSFLRGFPHANTRLVLFEADIY 65
VCVTG G+IGS LV +L+E+GY V AT++ D KV L P A T L ++ AD+
Sbjct: 68 VCVTGSTGFIGSWLVMRLMERGYMVRATVQRDPDNMKKVKHLLELPGAKTNLTIWNADLT 247
Query: 66 KPDDFGTAIQGCEFVFHVATP 86
+ F AI+GC VFHVA+P
Sbjct: 248 EEGSFDEAIKGCSGVFHVASP 310
>TC10850 homologue to UP|Q9S6Y4 (Q9S6Y4) Dihydroflavanol reductase 3,
partial (51%)
Length = 594
Score = 48.5 bits (114), Expect(2) = 1e-14
Identities = 26/53 (49%), Positives = 33/53 (62%)
Frame = +2
Query: 34 TLRNLKDESKVSFLRGFPHANTRLVLFEADIYKPDDFGTAIQGCEFVFHVATP 86
TL N+K KV L P A T+L L++AD+ + F AI+GC VFHVATP
Sbjct: 194 TLANMK---KVKHLLELPEAKTKLTLWKADLAEEGSFDEAIKGCTGVFHVATP 343
Score = 45.8 bits (107), Expect(2) = 1e-14
Identities = 20/31 (64%), Positives = 26/31 (83%)
Frame = +1
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRN 37
VCVTG AG+IGS LV +L+E+GY V AT+R+
Sbjct: 103 VCVTGAAGFIGSWLVMRLMERGYMVRATVRD 195
>TC16256 similar to UP|Q7Y0H8 (Q7Y0H8) Cinnamoyl CoA reductase, partial
(32%)
Length = 475
Score = 71.2 bits (173), Expect = 8e-14
Identities = 37/80 (46%), Positives = 47/80 (58%)
Frame = +3
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTG G+I S LVK LLE+GY V T+RN D+ K L+ A RL L +AD++
Sbjct: 144 VCVTGAGGFIASWLVKLLLERGYIVRGTVRN-PDDPKNGHLKELEGAKERLTLHKADLFD 320
Query: 67 PDDFGTAIQGCEFVFHVATP 86
D GC+ VFH A+P
Sbjct: 321 LDSLKAVFHGCDGVFHTASP 380
>TC19621 similar to UP|Q8VZH7 (Q8VZH7) Cinnamoyl-CoA reductase , partial
(20%)
Length = 485
Score = 69.3 bits (168), Expect = 3e-13
Identities = 37/95 (38%), Positives = 50/95 (51%)
Frame = +3
Query: 6 KVCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIY 65
KVCVTG G++ S LVK LL KGY L + K L A+ L LF+AD+
Sbjct: 30 KVCVTGAGGFVASWLVKLLLSKGYHCSWXLSDXPGSPKYEHLLKLEKASENLTLFKADLL 209
Query: 66 KPDDFGTAIQGCEFVFHVATPYLHQTDSQVNIQIL 100
+ +AI GC VFHVA+P T S ++++
Sbjct: 210 NYESVHSAILGCTAVFHVASPVPSTTSSNPEVEVI 314
>TC7966 similar to UP|Q84V25 (Q84V25) Quinone oxidoreductase, partial (98%)
Length = 1908
Score = 68.6 bits (166), Expect = 5e-13
Identities = 36/89 (40%), Positives = 50/89 (55%)
Frame = -1
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
+CVTG G+I S +VK LL+KGYTV T+RN D+ K LR A+ RL L + D+ +
Sbjct: 588 ICVTGAGGFIASWMVKLLLQKGYTVRGTVRN-PDDPKNGHLRELEGASDRLTLIKVDLLE 412
Query: 67 PDDFGTAIQGCEFVFHVATPYLHQTDSQV 95
+ A+ G VFH A+P + V
Sbjct: 411 LNSVRAAVHGSHGVFHTASPVTDNPEEMV 325
>AV774784
Length = 464
Score = 49.3 bits (116), Expect(2) = 2e-09
Identities = 21/42 (50%), Positives = 31/42 (73%)
Frame = +1
Query: 6 KVCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFL 47
+ CV GG G++ SLL+K+LLEKGY V+ T+R+ + K+S L
Sbjct: 64 EACVIGGTGFVASLLIKQLLEKGYAVNTTVRDPDNHKKISQL 189
Score = 27.3 bits (59), Expect(2) = 2e-09
Identities = 13/41 (31%), Positives = 21/41 (50%)
Frame = +2
Query: 59 LFEADIYKPDDFGTAIQGCEFVFHVATPYLHQTDSQVNIQI 99
+F ++ +DF T I G + VF +ATP ++ N I
Sbjct: 221 IFGGELTVENDFDTPIAGSDLVFQLATPVNFASEDPENDMI 343
>TC8549 weakly similar to UP|Q8LEH3 (Q8LEH3) Cinnamoyl-CoA reductase-like
protein, partial (17%)
Length = 758
Score = 48.1 bits (113), Expect = 7e-07
Identities = 26/86 (30%), Positives = 46/86 (53%)
Frame = +3
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCV +G +G LV+KLL++GYTVHA+++ + ++ + +L +F +D +
Sbjct: 156 VCVMDASGQLGFRLVQKLLQRGYTVHASIQKYGVDKEM--FNEISADSDKLKVFRSDPFD 329
Query: 67 PDDFGTAIQGCEFVFHVATPYLHQTD 92
A++GC +F+ P Q D
Sbjct: 330 YHSLIDALKGCSGLFYTFEPPFDQPD 407
>TC13250 weakly similar to UP|Q7NJT5 (Q7NJT5) Glr1747 protein, partial (13%)
Length = 575
Score = 44.7 bits (104), Expect = 8e-06
Identities = 29/83 (34%), Positives = 37/83 (43%)
Frame = +3
Query: 2 ERRCKVCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFE 61
E+ KV VTG GY+G L LL +GY+V R D L P + +F
Sbjct: 180 EKMNKVSVTGATGYLGGRLCHALLRQGYSVRILARRTSD------LSALPPPSE---IFY 332
Query: 62 ADIYKPDDFGTAIQGCEFVFHVA 84
DI A+ GC VFH+A
Sbjct: 333 GDITDFPSLLAAVSGCSVVFHLA 401
>AV428109
Length = 168
Score = 42.0 bits (97), Expect = 5e-05
Identities = 17/31 (54%), Positives = 24/31 (76%)
Frame = +3
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRN 37
VCVTG GY+ S L+ +LL+ GY+V AT+R+
Sbjct: 27 VCVTGATGYVASWLIMRLLQHGYSVRATVRS 119
>BP035620
Length = 523
Score = 40.8 bits (94), Expect = 1e-04
Identities = 23/51 (45%), Positives = 29/51 (56%)
Frame = +2
Query: 8 CVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLV 58
CVTG +GY+ S L+K+LL GY V T+R+L S L H N LV
Sbjct: 29 CVTGASGYLASWLIKRLLLSGYHVVGTVRDLGSFPSPSLL----HQNLELV 169
>TC17328 weakly similar to UP|Q9FGH3 (Q9FGH3) Dihydroflavonol
4-reductase-like (Cinnamoyl-CoA reductase-like
protein), partial (21%)
Length = 479
Score = 38.1 bits (87), Expect = 7e-04
Identities = 21/57 (36%), Positives = 30/57 (51%)
Frame = +3
Query: 30 TVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYKPDDFGTAIQGCEFVFHVATP 86
T+HAT D + + + P A++R+ +F ADI AI C VFHVA+P
Sbjct: 3 TIHATTFPSSDPTHLFTVH--PEAHSRITIFPADILDSAAVSRAIHNCSGVFHVASP 167
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.142 0.436
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,357,137
Number of Sequences: 28460
Number of extensions: 54487
Number of successful extensions: 437
Number of sequences better than 10.0: 85
Number of HSP's better than 10.0 without gapping: 405
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 429
length of query: 162
length of database: 4,897,600
effective HSP length: 83
effective length of query: 79
effective length of database: 2,535,420
effective search space: 200298180
effective search space used: 200298180
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 52 (24.6 bits)
Medicago: description of AC129091.12