BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC128638.4 - phase: 0
(561 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CB829559 340 3e-94
AV407645 47 8e-06
TC12572 similar to PIR|T46032|T46032 WD-40 repeat regulatory pro... 45 2e-05
TC13023 similar to UP|Q8L830 (Q8L830) WD40-repeat protein (Fragm... 45 3e-05
TC19491 similar to GB|AAP37692.1|30725340|BT008333 At4g29830 {Ar... 42 2e-04
TC16076 weakly similar to GB|AAG32022.1|11141605|AF277458 LEUNIG... 40 8e-04
TC14937 similar to UP|Q9FN19 (Q9FN19) Genomic DNA, chromosome 5,... 40 0.001
BP038344 39 0.002
TC9407 weakly similar to UP|TAF5_YEAST (P38129) Transcription in... 38 0.005
TC7876 homologue to UP|GBLP_SOYBN (Q39836) Guanine nucleotide-bi... 37 0.006
AU089179 36 0.014
AV409882 35 0.025
AV767790 35 0.032
TC19190 weakly similar to PIR|T46032|T46032 WD-40 repeat regulat... 34 0.072
TC11888 similar to UP|O22636 (O22636) Poly(A) polymerase (Pepti... 33 0.12
TC9693 similar to UP|COPP_MOUSE (O55029) Coatomer beta' subunit ... 33 0.16
TC15844 homologue to UP|Q9FV61 (Q9FV61) Heterotrimeric GTP-bindi... 32 0.21
BP058074 32 0.27
TC10816 homologue to UP|CAE45585 (CAE45585) Coatomer alpha subun... 31 0.46
TC12798 similar to UP|O39876 (O39876) HBc antigen, partial (8%) 31 0.46
>CB829559
Length = 552
Score = 340 bits (873), Expect = 3e-94
Identities = 166/186 (89%), Positives = 170/186 (91%)
Frame = +1
Query: 375 VWSMEDRKVVAWGEGHNSWVSGVAFDSYWSSPNSNDNGETITYRFGSVGQDTQLLLWELE 434
VWSMEDRKVVAWGEGHNSWVSGVAFDSYWSSPNSNDNGETI YRFGSVGQDTQLLLW+LE
Sbjct: 1 VWSMEDRKVVAWGEGHNSWVSGVAFDSYWSSPNSNDNGETIMYRFGSVGQDTQLLLWDLE 180
Query: 435 MDEIVVPLRRGPPGGSPTFGSPTFSAGSQSSHWDNAVPLGTLQPAPSMRDVPKISPLVAH 494
MDEIVVPLRR PPGGSPT+ S GSQSSHWDN VPLGTLQPAPSMRDVPKISPLVAH
Sbjct: 181 MDEIVVPLRRCPPGGSPTY-----STGSQSSHWDNVVPLGTLQPAPSMRDVPKISPLVAH 345
Query: 495 RVHTEPLSSLIFTQESVLTACREGHIKIWTRPGVAESQPSNSETLLATSLKEKPSLSSKI 554
RVHTEPLS LIFTQESVLTACREGHIK+W RPG ESQPSNSET LATSLKEKP LSSK+
Sbjct: 346 RVHTEPLSGLIFTQESVLTACREGHIKVWMRPGAPESQPSNSETSLATSLKEKPLLSSKL 525
Query: 555 SNSIYK 560
SNS YK
Sbjct: 526 SNSSYK 543
>AV407645
Length = 413
Score = 47.0 bits (110), Expect = 8e-06
Identities = 24/80 (30%), Positives = 43/80 (53%)
Frame = +2
Query: 307 QGSINSISFSADGAYLATVGRDGYLRVFDYTKEHLVCGGKSYYGGLLCCAWSMDGKYILT 366
Q + +SIS+S + L T G + +R +D + + + GL+ C W GKYIL+
Sbjct: 140 QKAFSSISWSPNDQELLTCGVEEAIRRWDVSTGKCLQIYEKAGAGLISCTWFPSGKYILS 319
Query: 367 GGEDDLVQVWSMEDRKVVAW 386
G D + +W ++ ++V +W
Sbjct: 320 GLSDKSICMWELDGKEVESW 379
>TC12572 similar to PIR|T46032|T46032 WD-40 repeat regulatory protein tup1
homolog - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (51%)
Length = 770
Score = 45.4 bits (106), Expect = 2e-05
Identities = 29/130 (22%), Positives = 58/130 (44%), Gaps = 3/130 (2%)
Frame = +3
Query: 310 INSISFSADGAYLATVGRDGYLRVFDYTKEHLVCGGKSYYGGLLCCAWSM---DGKYILT 366
++ + FS + ++ D LR+++Y+ + + C + + +GKY++
Sbjct: 177 VSFVKFSPNAKFILVGTLDNNLRLWNYSTGRFLKTYTGHVNSKYCISSTFSTTNGKYVVG 356
Query: 367 GGEDDLVQVWSMEDRKVVAWGEGHNSWVSGVAFDSYWSSPNSNDNGETITYRFGSVGQDT 426
G ED + +W ++ RK+V EGH+ V V+ P N G++G D
Sbjct: 357 GSEDHGIYLWELQTRKIVQKLEGHSDTVVSVS-----CHPTEN------MIASGALGNDK 503
Query: 427 QLLLWELEMD 436
+ +W + D
Sbjct: 504 TVKIWTQQKD 533
>TC13023 similar to UP|Q8L830 (Q8L830) WD40-repeat protein (Fragment),
partial (18%)
Length = 591
Score = 45.1 bits (105), Expect = 3e-05
Identities = 26/91 (28%), Positives = 43/91 (46%)
Frame = +1
Query: 309 SINSISFSADGAYLATVGRDGYLRVFDYTKEHLVCGGKSYYGGLLCCAWSMDGKYILTGG 368
S+ +++ S D L + G +RV+D + V K + G ++C + G + TGG
Sbjct: 289 SVTALALSPDDNLLFSSGHSRQIRVWDLSTLKCVRSWKGHEGPVMCMSCHPSGGLLATGG 468
Query: 369 EDDLVQVWSMEDRKVVAWGEGHNSWVSGVAF 399
D V VW ++ + +GH VS V F
Sbjct: 469 ADRKVLVWDVDGGYCTHFFKGHGGVVSCVMF 561
>TC19491 similar to GB|AAP37692.1|30725340|BT008333 At4g29830 {Arabidopsis
thaliana;}, partial (39%)
Length = 527
Score = 42.4 bits (98), Expect = 2e-04
Identities = 29/91 (31%), Positives = 41/91 (44%), Gaps = 1/91 (1%)
Frame = +1
Query: 310 INSISFSA-DGAYLATVGRDGYLRVFDYTKEHLVCGGKSYYGGLLCCAWSMDGKYILTGG 368
+ S+ +S D L T DG + ++D + LV + +LC S DG I TG
Sbjct: 25 VRSLVYSPYDPRLLFTASDDGNVHMYDAEGKALVGTMSGHASWVLCVDVSPDGGAIATGS 204
Query: 369 EDDLVQVWSMEDRKVVAWGEGHNSWVSGVAF 399
D V++W + R V H V GVAF
Sbjct: 205 SDRTVRLWDLAMRASVQTMSNHTDQVWGVAF 297
>TC16076 weakly similar to GB|AAG32022.1|11141605|AF277458 LEUNIG
{Arabidopsis thaliana;} , partial (24%)
Length = 1048
Score = 40.4 bits (93), Expect = 8e-04
Identities = 23/79 (29%), Positives = 38/79 (47%)
Frame = +3
Query: 354 CCAWSMDGKYILTGGEDDLVQVWSMEDRKVVAWGEGHNSWVSGVAFDSYWSSPNSNDNGE 413
CC +S DGK + + G+D V +W+M+ + + E H S +S V F PNS+
Sbjct: 9 CCHFSSDGKMLASAGDDKKVVLWNMDTLQTESTPEQHKSVISDVRF-----RPNSS---- 161
Query: 414 TITYRFGSVGQDTQLLLWE 432
+ + D + LW+
Sbjct: 162 ----QLATASIDKSVRLWD 206
>TC14937 similar to UP|Q9FN19 (Q9FN19) Genomic DNA, chromosome 5, TAC
clone:K8K14 (AT5g67320/K8K14_4), partial (19%)
Length = 1089
Score = 40.0 bits (92), Expect = 0.001
Identities = 25/100 (25%), Positives = 47/100 (47%)
Frame = +1
Query: 322 LATVGRDGYLRVFDYTKEHLVCGGKSYYGGLLCCAWSMDGKYILTGGEDDLVQVWSMEDR 381
LA+ D ++++D L+ + G+ A+S +G+Y+ +G D + +WS+++
Sbjct: 55 LASASFDSTVKLWDVEVGKLIYSLNGHRDGVYSVAFSPNGEYLASGSPDKSIHIWSLKEG 234
Query: 382 KVVAWGEGHNSWVSGVAFDSYWSSPNSNDNGETITYRFGS 421
K+V G SG F+ W N G+ I F +
Sbjct: 235 KIVKTYTG-----SGGIFEVCW-----NKEGDKIAACFAN 324
>BP038344
Length = 525
Score = 38.9 bits (89), Expect = 0.002
Identities = 27/101 (26%), Positives = 42/101 (40%)
Frame = +2
Query: 309 SINSISFSADGAYLATVGRDGYLRVFDYTKEHLVCGGKSYYGGLLCCAWSMDGKYILTGG 368
++ + FS DG LA+ D L ++ L+ + G+ AWS D YI +
Sbjct: 104 AVACVKFSNDGTLLASASLDKTLIIWSSATLSLLHRLTGHSEGISDLAWSSDSHYICSAS 283
Query: 369 EDDLVQVWSMEDRKVVAWGEGHNSWVSGVAFDSYWSSPNSN 409
+D +++W V GH V V F +P SN
Sbjct: 284 DDRTLRIWDATGGDCVKTLRGHTHAVFCVNF-----NPQSN 391
>TC9407 weakly similar to UP|TAF5_YEAST (P38129) Transcription initiation
factor TFIID subunit 5 (TBP-associated factor 5)
(TBP-associated factor 90 kDa) (TAFII-90), partial (4%)
Length = 530
Score = 37.7 bits (86), Expect = 0.005
Identities = 21/83 (25%), Positives = 41/83 (49%), Gaps = 3/83 (3%)
Frame = +2
Query: 312 SISFSADGAYLATVGRDGYLRVFD---YTKEHLVCGGKSYYGGLLCCAWSMDGKYILTGG 368
S F YLAT D +++++ +T + + G + + C +S+DG Y++T
Sbjct: 29 SPEFCEPHRYLATASADHTVKIWNVDGFTLDKTLIGHQRWVWD---CVFSVDGAYLITAS 199
Query: 369 EDDLVQVWSMEDRKVVAWGEGHN 391
D ++WSM + + +GH+
Sbjct: 200 SDTTARLWSMSTGEDIKVYQGHH 268
Score = 26.9 bits (58), Expect = 8.8
Identities = 13/42 (30%), Positives = 20/42 (46%)
Frame = +2
Query: 315 FSADGAYLATVGRDGYLRVFDYTKEHLVCGGKSYYGGLLCCA 356
FS DGAYL T D R++ + + + ++ CCA
Sbjct: 164 FSVDGAYLITASSDTTARLWSMSTGEDIKVYQGHHKATTCCA 289
>TC7876 homologue to UP|GBLP_SOYBN (Q39836) Guanine nucleotide-binding
protein beta subunit-like protein, complete
Length = 1379
Score = 37.4 bits (85), Expect = 0.006
Identities = 19/81 (23%), Positives = 40/81 (48%), Gaps = 4/81 (4%)
Frame = +3
Query: 308 GSINSISFSADGAYLATVGRDGYLRVFDYTKEHLVCGGKSYY----GGLLCCAWSMDGKY 363
G +N+++ S DG+ A+ G+DG + ++D + GK Y G ++ +Y
Sbjct: 651 GYVNTVAVSPDGSLCASGGKDGVILLWDLAE------GKRLYSLDAGSIIHALCFSPNRY 812
Query: 364 ILTGGEDDLVQVWSMEDRKVV 384
L + +++W +E + +V
Sbjct: 813 WLCAATETSIKIWDLESKSIV 875
Score = 32.0 bits (71), Expect = 0.27
Identities = 29/126 (23%), Positives = 59/126 (46%), Gaps = 4/126 (3%)
Frame = +3
Query: 312 SISFSADGAYLATVGRDGYLRVFDYTKE--HLVCGGKSYYGGLLCCAWSMDGKY--ILTG 367
S++FS D + + RD +++++ E + + ++ + C +S I++
Sbjct: 399 SVAFSIDNRQIVSASRDRTIKLWNTLGECKYTIQDNDAHSDWVSCVRFSPSTLQPTIVSA 578
Query: 368 GEDDLVQVWSMEDRKVVAWGEGHNSWVSGVAFDSYWSSPNSNDNGETITYRFGSVGQDTQ 427
D V+VW++ + K+ GH+ +V+ VA SP+ + S G+D
Sbjct: 579 SWDRTVKVWNLTNCKLRNTLAGHSGYVNTVAV-----SPDGS--------LCASGGKDGV 719
Query: 428 LLLWEL 433
+LLW+L
Sbjct: 720 ILLWDL 737
Score = 28.5 bits (62), Expect = 3.0
Identities = 29/117 (24%), Positives = 47/117 (39%), Gaps = 7/117 (5%)
Frame = +3
Query: 322 LATVGRDGYLRVFDYTKEHLVCG-------GKSYYGGLLCCAWSMDGKYILTGGEDDLVQ 374
+ T RD + ++ TKE G G S++ + S DG++ L+G D ++
Sbjct: 162 IVTASRDKSIILWHLTKEDKTYGVPRRRLTGHSHF--VQDVVLSSDGQFALSGSWDGELR 335
Query: 375 VWSMEDRKVVAWGEGHNSWVSGVAFDSYWSSPNSNDNGETITYRFGSVGQDTQLLLW 431
+W + GH V VAF S DN + + S +D + LW
Sbjct: 336 LWDLAAGTSARRFVGHTKDVLSVAF--------SIDNRQIV-----SASRDRTIKLW 467
Score = 27.7 bits (60), Expect = 5.1
Identities = 14/51 (27%), Positives = 26/51 (50%), Gaps = 3/51 (5%)
Frame = +3
Query: 331 LRVFDYTKEHLVCGGKSYYGGLLCCA---WSMDGKYILTGGEDDLVQVWSM 378
L+V T+ GG + ++ C WS DG + +G D +++VW++
Sbjct: 882 LKVDLKTEADATTGGNTNKKKVIYCTSLNWSADGSTLFSGYTDGVIRVWAI 1034
>AU089179
Length = 340
Score = 36.2 bits (82), Expect = 0.014
Identities = 21/73 (28%), Positives = 39/73 (52%), Gaps = 1/73 (1%)
Frame = +3
Query: 307 QGSINSISFSADGAYLATVGRDGYLRVFDYTKEHLVC-GGKSYYGGLLCCAWSMDGKYIL 365
+G +++F+ DG LAT G DG LRVF + ++ ++ + +S +GK+I+
Sbjct: 6 EGQQLALAFNNDGTALATGGEDGSLRVFKWPXMKVILEHSTAHSSSVKDLHFSSNGKWIV 185
Query: 366 TGGEDDLVQVWSM 378
+ G +VW +
Sbjct: 186 SLGSGGPCRVWDL 224
>AV409882
Length = 422
Score = 35.4 bits (80), Expect = 0.025
Identities = 23/108 (21%), Positives = 47/108 (43%), Gaps = 1/108 (0%)
Frame = +3
Query: 294 YSKSNPIARWHICQGSINSISFSADGAYLATVGRDGYLRVFDYTKEHLVCGGKSYYGGLL 353
Y + + + + S F A ++ D ++RV++Y V +++ +
Sbjct: 57 YQSQTMAKSFEVTELPVRSAKFVARKQWVVAGADDMFIRVYNYNTMDKVKVFEAHTDYIR 236
Query: 354 CCAWSMDGKYILTGGEDDLVQVWSMEDRKVVAW-GEGHNSWVSGVAFD 400
C A Y+L+ +D L+++W E + EGH+ +V V F+
Sbjct: 237 CVAVHPTLPYVLSSSDDMLIKLWDWEKGWICTQIFEGHSHYVMQVTFN 380
>AV767790
Length = 617
Score = 35.0 bits (79), Expect = 0.032
Identities = 13/40 (32%), Positives = 24/40 (59%)
Frame = +3
Query: 352 LLCCAWSMDGKYILTGGEDDLVQVWSMEDRKVVAWGEGHN 391
++CC +S DGK + + G D V +W+ME + + + H+
Sbjct: 483 VVCCHFSSDGKLLASAGHDQKVVLWNMETLQTESTPDAHS 602
>TC19190 weakly similar to PIR|T46032|T46032 WD-40 repeat regulatory protein
tup1 homolog - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (18%)
Length = 511
Score = 33.9 bits (76), Expect = 0.072
Identities = 13/38 (34%), Positives = 23/38 (60%)
Frame = +3
Query: 360 DGKYILTGGEDDLVQVWSMEDRKVVAWGEGHNSWVSGV 397
+G+YI++G ED V +W ++ + ++ EGH V V
Sbjct: 12 NGRYIVSGSEDRCVYLWDLQQKNMIQKLEGHTDTVISV 125
>TC11888 similar to UP|O22636 (O22636) Poly(A) polymerase (Peptidyl-prolyl
cis-trans isomerase) (PPIase) (Rotamase) , partial (57%)
Length = 931
Score = 33.1 bits (74), Expect = 0.12
Identities = 28/97 (28%), Positives = 43/97 (43%), Gaps = 6/97 (6%)
Frame = -3
Query: 69 LAHLKEKPAPLTPTASSSSFSASSGVRSAAARLL--GGSNGNRAL-SFVGG---NGSSKS 122
L L + + LTP S +SSG ++ LL G + NRA S +GG N
Sbjct: 497 LVFLSTESSALTPAILRLSVISSSGFCTSLTALLSIGRAYLNRAPGSLIGGPDINTDKSD 318
Query: 123 NGGASRIGSIGSSSLSSSVANPNFDGKGSYLVFNAGD 159
N G + + S+G ++ + V NP + + F D
Sbjct: 317 NTGLAALASVGHPNVGTPVTNPTANAIDTAQCFKEND 207
>TC9693 similar to UP|COPP_MOUSE (O55029) Coatomer beta' subunit
(Beta'-coat protein) (Beta'-COP) (p102), partial (20%)
Length = 743
Score = 32.7 bits (73), Expect = 0.16
Identities = 22/108 (20%), Positives = 46/108 (42%), Gaps = 1/108 (0%)
Frame = +1
Query: 294 YSKSNPIARWHICQGSINSISFSADGAYLATVGRDGYLRVFDYTKEHLVCGGKSYYGGLL 353
Y + + + + S F A ++ D ++RV++Y V +++ +
Sbjct: 337 YQTQTMAKSFEVTELPVRSAKFIARKQWVVAGADDMFIRVYNYNTMDKVKVFEAHTDYIR 516
Query: 354 CCAWSMDGKYILTGGEDDLVQVWSMEDRKVVAW-GEGHNSWVSGVAFD 400
C A Y+L+ +D L+++W E + E H+ +V V F+
Sbjct: 517 CVAVHPTLPYVLSSSDDMLIKLWDWEKGWICTQICEAHSHYVMQVTFN 660
>TC15844 homologue to UP|Q9FV61 (Q9FV61) Heterotrimeric GTP-binding protein
subunit beta 1, partial (28%)
Length = 668
Score = 32.3 bits (72), Expect = 0.21
Identities = 21/72 (29%), Positives = 33/72 (45%), Gaps = 4/72 (5%)
Frame = +3
Query: 310 INSISFSADGAYLATVGRDGYLRVFDYTKEHLVCG----GKSYYGGLLCCAWSMDGKYIL 365
+ SI+FS G L +G V+D +V S+ G + C S DG +
Sbjct: 93 VTSIAFSISGRLLFAGYANGDCYVWDTLLAKVVLNIGSLQDSHEGRISCLGLSADGSALC 272
Query: 366 TGGEDDLVQVWS 377
TGG D +++W+
Sbjct: 273 TGGWDTNLKIWA 308
>BP058074
Length = 575
Score = 32.0 bits (71), Expect = 0.27
Identities = 24/78 (30%), Positives = 35/78 (44%), Gaps = 13/78 (16%)
Frame = -1
Query: 61 GKTVSMVTLAHLKEKPAPLTPTASSS------------SFSASSGVRSAAA-RLLGGSNG 107
GKT + L + PAP P A++ S SS RSAAA R+ S+
Sbjct: 311 GKTGCPIKLLRTADLPAPTPPRATTLRKVEGRLEDEELESSTSSSARSAAAWRIESASSD 132
Query: 108 NRALSFVGGNGSSKSNGG 125
+++ +G SK+ GG
Sbjct: 131 KSSIAAIGQPNGSKNGGG 78
>TC10816 homologue to UP|CAE45585 (CAE45585) Coatomer alpha subunit-like
protein, partial (20%)
Length = 979
Score = 31.2 bits (69), Expect = 0.46
Identities = 15/92 (16%), Positives = 39/92 (42%)
Frame = +1
Query: 308 GSINSISFSADGAYLATVGRDGYLRVFDYTKEHLVCGGKSYYGGLLCCAWSMDGKYILTG 367
G + + F + G D ++V++Y + + + + + +I++
Sbjct: 394 GPVRGVHFHHSQPLFVSGGDDYKIKVWNYKLHRCLFTLLGHLDYIRTVQFHHESPWIVSA 573
Query: 368 GEDDLVQVWSMEDRKVVAWGEGHNSWVSGVAF 399
+D +++W+ + R ++ GHN +V F
Sbjct: 574 SDDQTIRIWNWQSRTCISVLTGHNHYVMCALF 669
>TC12798 similar to UP|O39876 (O39876) HBc antigen, partial (8%)
Length = 735
Score = 31.2 bits (69), Expect = 0.46
Identities = 26/68 (38%), Positives = 33/68 (48%), Gaps = 1/68 (1%)
Frame = +2
Query: 78 PLTPTASSSSFSASSGVRSAAARLLGGSNGN-RALSFVGGNGSSKSNGGASRIGSIGSSS 136
P AS ++ +SSG AAAR+ S G+ A V S S G R GSI
Sbjct: 41 PREDAASVRTWKSSSGSVKAAARIAATSGGSVSARKKVEPRNVSGSRAGTKRSGSI--DG 214
Query: 137 LSSSVANP 144
L+SSV+ P
Sbjct: 215 LASSVSVP 238
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.313 0.131 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,765,470
Number of Sequences: 28460
Number of extensions: 173279
Number of successful extensions: 1058
Number of sequences better than 10.0: 88
Number of HSP's better than 10.0 without gapping: 956
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1026
length of query: 561
length of database: 4,897,600
effective HSP length: 95
effective length of query: 466
effective length of database: 2,193,900
effective search space: 1022357400
effective search space used: 1022357400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC128638.4