BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC127428.5 + phase: 0
(851 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP042557 314 7e-90
AV410062 290 5e-79
TC19318 similar to UP|Q94JM3 (Q94JM3) AT5g62000/mtg10_20, partia... 198 3e-51
AV409033 189 2e-48
AV779571 167 8e-42
BP049491 145 2e-35
BP043161 114 6e-26
TC10194 similar to UP|Q8S979 (Q8S979) Auxin response factor 7a (... 100 7e-22
TC10030 similar to UP|Q94JM3 (Q94JM3) AT5g62000/mtg10_20, partia... 100 7e-22
TC13094 similar to UP|Q94JM3 (Q94JM3) AT5g62000/mtg10_20, partia... 100 1e-21
AV779806 97 8e-21
TC14787 similar to UP|Q9ZTX8 (Q9ZTX8) ARF6 (Auxin response facto... 84 7e-17
TC12865 similar to UP|Q9ZTX8 (Q9ZTX8) ARF6 (Auxin response facto... 71 8e-13
AV781081 64 1e-10
AV408921 59 2e-09
AV779960 54 8e-08
AI967501 52 3e-07
BP055405 49 4e-06
AW720417 47 1e-05
TC16093 weakly similar to UP|PF21_ARATH (Q04088) Possible transc... 47 2e-05
>BP042557
Length = 545
Score = 314 bits (805), Expect(2) = 7e-90
Identities = 156/168 (92%), Positives = 163/168 (96%), Gaps = 1/168 (0%)
Frame = +2
Query: 110 SKQPTN-YFCKTLTASDTSTHGGFSVPRRAAEKVFPPLDFSQQPPAQELIARDLHGNEWK 168
SKQ TN YFCKTLTASDTSTHGGFSVPRRAAEKVFPPLD+SQQPPAQELIARDL+ NEWK
Sbjct: 41 SKQTTNNYFCKTLTASDTSTHGGFSVPRRAAEKVFPPLDYSQQPPAQELIARDLNDNEWK 220
Query: 169 FRHIFRGQPKRHLLTTGWSVFVSAKRLVAGDSVLFIWNEKNQLLLGIRRASRPQTVMPSS 228
FRHIFRGQPKRHLLTTGWSVFVSAKRLVAGDSVLFIWNEKNQLLLGIRRA+R QT+MPSS
Sbjct: 221 FRHIFRGQPKRHLLTTGWSVFVSAKRLVAGDSVLFIWNEKNQLLLGIRRANRSQTIMPSS 400
Query: 229 VLSSDSMHLGLLAAAAHAAATNSRFTIFYNPRACPSEFVIPLAKYVKA 276
VLSSDSMH+GLLAAAAHAA+TNSRFTIFYNPRA PSEFVIPLA+YVKA
Sbjct: 401 VLSSDSMHIGLLAAAAHAASTNSRFTIFYNPRASPSEFVIPLAQYVKA 544
Score = 34.7 bits (78), Expect(2) = 7e-90
Identities = 17/19 (89%), Positives = 17/19 (89%), Gaps = 1/19 (5%)
Frame = +3
Query: 97 QKEAYL-PAELGTPSKQPT 114
QKE YL PAELGTPSKQPT
Sbjct: 3 QKEVYLLPAELGTPSKQPT 59
>AV410062
Length = 435
Score = 290 bits (743), Expect = 5e-79
Identities = 140/144 (97%), Positives = 142/144 (98%)
Frame = +3
Query: 22 LVSLPAVGSRVVYFPQGHSEQVAVSTNKEVDAHIPNYPSLPPQLICQLHNLTMHADVETD 81
LVSLPA+GSRVVYFPQGHSEQVAVSTNKEVDAHIPNYPSLPPQLICQLHN+TMHAD ETD
Sbjct: 3 LVSLPALGSRVVYFPQGHSEQVAVSTNKEVDAHIPNYPSLPPQLICQLHNVTMHADAETD 182
Query: 82 EVYAQMTLQPLNAQEQKEAYLPAELGTPSKQPTNYFCKTLTASDTSTHGGFSVPRRAAEK 141
EVYAQMTLQPLN QEQKEAYLPAELGTPSKQPTNYFCKTLTASDTSTHGGFSVPRRAAEK
Sbjct: 183 EVYAQMTLQPLNPQEQKEAYLPAELGTPSKQPTNYFCKTLTASDTSTHGGFSVPRRAAEK 362
Query: 142 VFPPLDFSQQPPAQELIARDLHGN 165
VFPPLDFSQQPPAQELIARDLHGN
Sbjct: 363 VFPPLDFSQQPPAQELIARDLHGN 434
>TC19318 similar to UP|Q94JM3 (Q94JM3) AT5g62000/mtg10_20, partial (23%)
Length = 585
Score = 198 bits (504), Expect = 3e-51
Identities = 98/172 (56%), Positives = 127/172 (72%)
Frame = +3
Query: 118 CKTLTASDTSTHGGFSVPRRAAEKVFPPLDFSQQPPAQELIARDLHGNEWKFRHIFRGQP 177
CKTLTASDTST GGFSV RR A++ PPLD S+QPP Q L+A+DLHG+EW+FRHIF GQP
Sbjct: 36 CKTLTASDTSTDGGFSVLRRHADECLPPLDMSKQPPTQALVAKDLHGSEWRFRHIFLGQP 215
Query: 178 KRHLLTTGWSVFVSAKRLVAGDSVLFIWNEKNQLLLGIRRASRPQTVMPSSVLSSDSMHL 237
+RHLL +GWSVFVS+KRLVAGD+ +F+ E +L +G+RRA R Q + SSV+SS S+HL
Sbjct: 216 RRHLLLSGWSVFVSSKRLVAGDAFIFLRGENGELRVGVRRAMRQQGNVSSSVISSPSLHL 395
Query: 238 GLLAAAAHAAATNSRFTIFYNPRACPSEFVIPLAKYVKAVYHTRVSVGMRFR 289
G+LA+A HA T S FT++Y PR PS ++ L + ++ MRF+
Sbjct: 396 GVLASAWHANLTGSMFTVYY*PRTSPSCNLLFLMINTWSPLPNNYTIAMRFK 551
>AV409033
Length = 418
Score = 189 bits (479), Expect = 2e-48
Identities = 92/132 (69%), Positives = 110/132 (82%), Gaps = 1/132 (0%)
Frame = +3
Query: 5 GEKRVLDSELWHACAGPLVSLPAVGSRVVYFPQGHSEQVAVSTNKEVDAHIPNYPSLPPQ 64
GE++ ++SELWHACAGPLVSLP VGS VVYFPQGHSEQVA S KE D IP+YP+LP +
Sbjct: 27 GERKTINSELWHACAGPLVSLPPVGSLVVYFPQGHSEQVAASMQKETD-FIPSYPNLPSK 203
Query: 65 LICQLHNLTMHADVETDEVYAQMTLQPLNAQEQKEAYLPAELG-TPSKQPTNYFCKTLTA 123
LIC LHN+ +HAD ETDEVYAQMTLQP+N + K+A L +++G ++QPT +FCKTLTA
Sbjct: 204 LICMLHNVALHADPETDEVYAQMTLQPVNKYD-KDAILASDMGLKQNRQPTEFFCKTLTA 380
Query: 124 SDTSTHGGFSVP 135
SDTSTHGGFSVP
Sbjct: 381 SDTSTHGGFSVP 416
>AV779571
Length = 538
Score = 167 bits (422), Expect = 8e-42
Identities = 109/174 (62%), Positives = 122/174 (69%), Gaps = 10/174 (5%)
Frame = +1
Query: 449 LPGSLL--QFQQPPNFPNRTAAL-MQAQMLQQSQPQQAFQNNNQENQNLSQSQPQAQTNP 505
LP S+L F + +RT L +QAQM QQSQPQQ FQ NNQENQ L QS Q++
Sbjct: 40 LPNSILVPYFSFSKHRTSRTELL*VQAQM*QQSQPQQVFQ-NNQENQQLLQS----QSHL 204
Query: 506 QQHPQHQHSFNNQLHHHS-------QQQQQTQQQVVDNNQQISGSVSTMSQFVSATQPQS 558
QQH QHQHSFN Q +H+ QQQQQTQQQVVD NQQI + STMSQFVSA QPQS
Sbjct: 205 QQHLQHQHSFNGQTQNHNHNQQQQQQQQQQTQQQVVD-NQQIGNAGSTMSQFVSAPQPQS 381
Query: 559 PPPMQALSSLCHQQSFSDSNVNSSTTIVSPLHSIMGSSFPHDESSLLMSLPRTS 612
P QA SSL QQSFSDSNV TTIVSPLHS+MG SFP DE+S L++L RTS
Sbjct: 382 -SPTQAQSSLSQQQSFSDSNVKPVTTIVSPLHSLMG-SFPQDETSQLLNLSRTS 537
Score = 49.3 bits (116), Expect = 3e-06
Identities = 27/36 (75%), Positives = 28/36 (77%)
Frame = +3
Query: 432 AAAALQDMRTVVDPSKQLPGSLLQFQQPPNFPNRTA 467
AAAA MR+V DPSKQ P SLLQFQQ NFPNRTA
Sbjct: 3 AAAAHSGMRSV-DPSKQHPSSLLQFQQTQNFPNRTA 107
>BP049491
Length = 516
Score = 145 bits (367), Expect = 2e-35
Identities = 68/127 (53%), Positives = 92/127 (71%)
Frame = -3
Query: 134 VPRRAAEKVFPPLDFSQQPPAQELIARDLHGNEWKFRHIFRGQPKRHLLTTGWSVFVSAK 193
VPRRAAE PPLD+ QQ P+QEL+A+DLHG EWKF HI+RG P+ HLLTTGWS+FVS
Sbjct: 514 VPRRAAEDCLPPLDYKQQRPSQELVAKDLHGVEWKFCHIYRG*PRWHLLTTGWSIFVSQN 335
Query: 194 RLVAGDSVLFIWNEKNQLLLGIRRASRPQTVMPSSVLSSDSMHLGLLAAAAHAAATNSRF 253
LV+GD+V F+ E +L LGIRR+ P+ +P S + + + + +L+ A+A +T S F
Sbjct: 334 NLVSGDAVSFLRGENGELRLGIRRSVGPRNGLPESTVGNQNSYPHILSVVANAISTRSMF 155
Query: 254 TIFYNPR 260
+FY+PR
Sbjct: 154 YVFYSPR 134
>BP043161
Length = 509
Score = 114 bits (285), Expect = 6e-26
Identities = 50/62 (80%), Positives = 57/62 (91%)
Frame = +2
Query: 5 GEKRVLDSELWHACAGPLVSLPAVGSRVVYFPQGHSEQVAVSTNKEVDAHIPNYPSLPPQ 64
GEK+ L+SELWHACAGPLVSLP G+RVVYFPQGHSEQV+ +TN+EVD H+PNYPSLPPQ
Sbjct: 323 GEKKCLNSELWHACAGPLVSLPTAGTRVVYFPQGHSEQVSATTNREVDGHMPNYPSLPPQ 502
Query: 65 LI 66
LI
Sbjct: 503 LI 508
>TC10194 similar to UP|Q8S979 (Q8S979) Auxin response factor 7a (Fragment),
partial (13%)
Length = 1293
Score = 100 bits (250), Expect = 7e-22
Identities = 52/117 (44%), Positives = 70/117 (59%), Gaps = 7/117 (5%)
Frame = +1
Query: 723 SSSYMNTAGADSSLNHGVTPSIGESGFLHTQENGEQGNNP-------LNKTFVKVYKSGS 775
SS T G + + +I +S FL+ G Q P +T+ KVYK G+
Sbjct: 10 SSMVSQTFGVPDMTFNSIDSTIDDSSFLNGDPWGPQPAPPPPPAQFQRMRTYTKVYKRGA 189
Query: 776 FGRSLDITKFSSYNELRSELARMFGLEGELEDPVRSGWQLVFVDRENDVLLLGDGPW 832
GRS+DI ++S Y EL+ +LAR FG+EG+LED R GW+LV+VD E DVLL+GD PW
Sbjct: 190 VGRSIDIARYSGYEELKQDLARRFGIEGQLEDRQRIGWKLVYVDHEGDVLLVGDDPW 360
>TC10030 similar to UP|Q94JM3 (Q94JM3) AT5g62000/mtg10_20, partial (13%)
Length = 588
Score = 100 bits (250), Expect = 7e-22
Identities = 45/79 (56%), Positives = 61/79 (76%)
Frame = +3
Query: 13 ELWHACAGPLVSLPAVGSRVVYFPQGHSEQVAVSTNKEVDAHIPNYPSLPPQLICQLHNL 72
ELWHACAGPLV++P G RV YFPQGH EQV STN+ + H+P Y LPP+++C++ N+
Sbjct: 354 ELWHACAGPLVTVPREGERVFYFPQGHIEQVEASTNQVAELHMPVY-DLPPKILCRVINV 530
Query: 73 TMHADVETDEVYAQMTLQP 91
+ A+ +TDEV+AQ+TL P
Sbjct: 531 MLKAEPDTDEVFAQVTLLP 587
>TC13094 similar to UP|Q94JM3 (Q94JM3) AT5g62000/mtg10_20, partial (18%)
Length = 685
Score = 100 bits (248), Expect = 1e-21
Identities = 46/85 (54%), Positives = 63/85 (74%)
Frame = +2
Query: 13 ELWHACAGPLVSLPAVGSRVVYFPQGHSEQVAVSTNKEVDAHIPNYPSLPPQLICQLHNL 72
ELWHACAGPLV++P G RV YFPQGH EQV STN+ D H+P Y LP +++C++ N+
Sbjct: 374 ELWHACAGPLVTVPREGERVFYFPQGHIEQVEASTNQVADEHMPVY-DLPSKILCRVINV 550
Query: 73 TMHADVETDEVYAQMTLQPLNAQEQ 97
+ A+ +TDEV+AQ+TL P Q++
Sbjct: 551 QLKAEPDTDEVFAQVTLLPEPNQDE 625
>AV779806
Length = 546
Score = 97.4 bits (241), Expect = 8e-21
Identities = 45/53 (84%), Positives = 49/53 (91%)
Frame = +2
Query: 5 GEKRVLDSELWHACAGPLVSLPAVGSRVVYFPQGHSEQVAVSTNKEVDAHIPN 57
GEK+ L+SEL HACAGPLVSLP +GSRVVYFPQGHSEQVA STN+EVDAHIPN
Sbjct: 386 GEKKCLNSELGHACAGPLVSLPPIGSRVVYFPQGHSEQVAASTNREVDAHIPN 544
>TC14787 similar to UP|Q9ZTX8 (Q9ZTX8) ARF6 (Auxin response factor 6),
partial (8%)
Length = 614
Score = 84.3 bits (207), Expect = 7e-17
Identities = 38/39 (97%), Positives = 39/39 (99%)
Frame = +2
Query: 795 LARMFGLEGELEDPVRSGWQLVFVDRENDVLLLGDGPWP 833
LARMFGLEGELEDPVRSGWQLVFVDRE+DVLLLGDGPWP
Sbjct: 2 LARMFGLEGELEDPVRSGWQLVFVDRESDVLLLGDGPWP 118
>TC12865 similar to UP|Q9ZTX8 (Q9ZTX8) ARF6 (Auxin response factor 6),
partial (6%)
Length = 659
Score = 70.9 bits (172), Expect = 8e-13
Identities = 33/41 (80%), Positives = 37/41 (89%), Gaps = 2/41 (4%)
Frame = +2
Query: 794 ELARMFGLEGELEDPV--RSGWQLVFVDRENDVLLLGDGPW 832
ELAR+FG EG+LEDP+ RSGWQLVFVDREND+LLLGD PW
Sbjct: 2 ELARIFGPEGQLEDPMTLRSGWQLVFVDRENDILLLGDDPW 124
>AV781081
Length = 528
Score = 63.9 bits (154), Expect = 1e-10
Identities = 28/38 (73%), Positives = 31/38 (80%)
Frame = +2
Query: 10 LDSELWHACAGPLVSLPAVGSRVVYFPQGHSEQVAVST 47
++ ELWHACAGPLVSLP +GS YFPQGH EQVA ST
Sbjct: 407 INPELWHACAGPLVSLPQLGSLAYYFPQGHIEQVAAST 520
>AV408921
Length = 429
Score = 59.3 bits (142), Expect = 2e-09
Identities = 26/37 (70%), Positives = 28/37 (75%)
Frame = +2
Query: 10 LDSELWHACAGPLVSLPAVGSRVVYFPQGHSEQVAVS 46
L ELWHACAGPLV+LP G RV YFPQGH EQ+ S
Sbjct: 317 LYKELWHACAGPLVTLPREGERVYYFPQGHMEQLEAS 427
>AV779960
Length = 497
Score = 54.3 bits (129), Expect = 8e-08
Identities = 22/24 (91%), Positives = 24/24 (99%)
Frame = -3
Query: 810 RSGWQLVFVDRENDVLLLGDGPWP 833
RSGWQLVFVD+ENDVL+LGDGPWP
Sbjct: 495 RSGWQLVFVDQENDVLVLGDGPWP 424
>AI967501
Length = 393
Score = 52.4 bits (124), Expect = 3e-07
Identities = 26/75 (34%), Positives = 45/75 (59%), Gaps = 1/75 (1%)
Frame = +1
Query: 758 QGNNPLNKTFVKVYKSG-SFGRSLDITKFSSYNELRSELARMFGLEGELEDPVRSGWQLV 816
Q ++ KV G + GR++D+ Y++L EL ++F L+G+L+ R+ W++V
Sbjct: 169 QSKQICGRSRTKVQMQGVAVGRAVDLNTLDGYDQLIDELEQLFDLKGQLQH--RNKWEIV 342
Query: 817 FVDRENDVLLLGDGP 831
F D E D++L+GD P
Sbjct: 343 FTDDEGDMMLVGDDP 387
>BP055405
Length = 512
Score = 48.5 bits (114), Expect = 4e-06
Identities = 28/58 (48%), Positives = 35/58 (60%), Gaps = 1/58 (1%)
Frame = -3
Query: 330 DESTAGERQPRVSLWEIEPLTT-FPMYPSPFPLRLKRPWPPGLPSFHGMKDDDFGMSS 386
DEST GE RVS+W IEP+ T F YP P LR K PW PG+P DD++ + +
Sbjct: 405 DESTTGEHXSRVSIW*IEPVVTPFYGYP-PLFLRPKFPWXPGMP------DDEYDLEN 253
>AW720417
Length = 554
Score = 47.0 bits (110), Expect = 1e-05
Identities = 28/86 (32%), Positives = 35/86 (40%)
Frame = +3
Query: 476 QQSQPQQAFQNNNQENQNLSQSQPQAQTNPQQHPQHQHSFNNQLHHHSQQQQQTQQQVVD 535
QQ Q Q Q + NQ Q Q PQ H QHQH+ QQQQQ QQQ +
Sbjct: 36 QQQQQHQYVQRHMMPNQQQYHQQQHQQNQPQYHQQHQHN--------QQQQQQQQQQWLR 191
Query: 536 NNQQISGSVSTMSQFVSATQPQSPPP 561
Q + + + Q +S P
Sbjct: 192 RTQLGGNDTNVVEEVEKTVQSESADP 269
>TC16093 weakly similar to UP|PF21_ARATH (Q04088) Possible transcription
factor PosF21 (AtbZIP59), partial (17%)
Length = 570
Score = 46.6 bits (109), Expect = 2e-05
Identities = 30/83 (36%), Positives = 40/83 (48%), Gaps = 4/83 (4%)
Frame = +1
Query: 481 QQAFQNNNQENQNLSQSQ-PQAQTNPQQHPQHQHSFNNQLHHHSQQQQ---QTQQQVVDN 536
QQ + +N+ + L+ Q Q Q +PQ+H QHQH F + QQQQ Q QQ +
Sbjct: 37 QQFYPHNHSMHTLLAAQQFQQLQIHPQKHQQHQHQFQQPIQQQQQQQQQPIQQPQQQMQQ 216
Query: 537 NQQISGSVSTMSQFVSATQPQSP 559
QQ S + SAT SP
Sbjct: 217 QQQEQHQQSGDLKMRSATSSPSP 285
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.130 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,032,229
Number of Sequences: 28460
Number of extensions: 249355
Number of successful extensions: 2422
Number of sequences better than 10.0: 145
Number of HSP's better than 10.0 without gapping: 2131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2283
length of query: 851
length of database: 4,897,600
effective HSP length: 98
effective length of query: 753
effective length of database: 2,108,520
effective search space: 1587715560
effective search space used: 1587715560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 59 (27.3 bits)
Medicago: description of AC127428.5