BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC127169.10 + phase: 1 /pseudo/partial
(367 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC16286 similar to UP|Q8GTP6 (Q8GTP6) Endo-1,4-beta-D-glucanase ... 55 2e-08
AV778020 53 9e-08
TC8688 homologue to UP|AAS45400 (AAS45400) Endo-1,4-beta-glucana... 47 7e-06
TC17987 similar to UP|O04890 (O04890) Endo-1,4-beta-glucanase ,... 36 7e-04
TC15878 homologue to UP|Q8L6S6 (Q8L6S6) Endo-beta-1,4-glucanase ... 35 0.015
AV780838 33 0.098
TC16159 homologue to UP|Q94A23 (Q94A23) AT5g64310/MSJ1_15, parti... 30 0.49
AU088935 29 1.1
TC16264 similar to GB|AAP21178.1|30102520|BT006370 At3g08040/T8G... 28 2.4
TC8646 similar to UP|Q941A9 (Q941A9) At1g26300/F28B23_4, partial... 28 2.4
TC16038 similar to UP|Q9LXT8 (Q9LXT8) Pre-mRNA splicing factor A... 28 3.1
TC18552 similar to UP|Q9FIM2 (Q9FIM2) Cell division protein FtsH... 28 3.1
TC9870 weakly similar to UP|NO16_MEDTR (P93328) Early nodulin 16... 27 7.0
TC17342 similar to PIR|JC7226|JC7226 endo-1,3(4)-beta-glucanase ... 27 7.0
CB826805 26 9.2
TC17787 26 9.2
>TC16286 similar to UP|Q8GTP6 (Q8GTP6) Endo-1,4-beta-D-glucanase , partial
(47%)
Length = 1076
Score = 54.7 bits (130), Expect = 2e-08
Identities = 28/63 (44%), Positives = 38/63 (59%), Gaps = 11/63 (17%)
Frame = +2
Query: 52 DHARELIKWGTDYLLLTFDSSASEINQIHAKVG-GSLKGSE----------KPEDMEYPR 100
+H +++IKWGTDY L TF++SA I+ I A+VG G G +PEDM+Y R
Sbjct: 782 NHVKDIIKWGTDYFLKTFNNSADTISTIAAQVGSGDTSGGSTTPNDHYCWMRPEDMDYDR 961
Query: 101 PTT 103
P T
Sbjct: 962 PVT 970
>AV778020
Length = 465
Score = 52.8 bits (125), Expect = 9e-08
Identities = 30/56 (53%), Positives = 38/56 (67%)
Frame = -3
Query: 312 DLRNNNSYTEPTLAGNAGLVAALISLTSTSTIGSGFDTDHTIFNQTRQYRPQNLPP 367
D R+N +YTEPTLAGNAG+VAAL+SLT+ + G D +TIF+ P N PP
Sbjct: 289 DSRSNYNYTEPTLAGNAGIVAALVSLTNIPS--HGIDR-NTIFSAIPPLGPPNPPP 131
>TC8688 homologue to UP|AAS45400 (AAS45400) Endo-1,4-beta-glucanase ,
partial (18%)
Length = 750
Score = 46.6 bits (109), Expect = 7e-06
Identities = 25/57 (43%), Positives = 36/57 (62%)
Frame = +3
Query: 311 YDLRNNNSYTEPTLAGNAGLVAALISLTSTSTIGSGFDTDHTIFNQTRQYRPQNLPP 367
+D+R N +YTEPT+AGNAGLVAAL++L+ +I +T+F+ P PP
Sbjct: 177 HDVRMNYNYTEPTIAGNAGLVAALVALSGDKSIPI---DKNTLFSAVPPMFPTPPPP 338
>TC17987 similar to UP|O04890 (O04890) Endo-1,4-beta-glucanase , partial
(25%)
Length = 501
Score = 36.2 bits (82), Expect(2) = 7e-04
Identities = 28/81 (34%), Positives = 39/81 (47%)
Frame = +1
Query: 219 WLPLRGNFEYVP*IY*PYHVLLTKIVVAIIKICRGIYTIEPWIPKFSPICC*CFIYGFSF 278
W+ + GNF + HVLL + R + +I+ W SPICC C + G+
Sbjct: 253 WVSI*GNFIGLSQSDQHNHVLLPTSFHQL**NQRRLDSIKSW*ASASPICCQCCLSGYPI 432
Query: 279 C*LYESPRCSWMVL*S*LLFN 299
*L +WMVL +* LFN
Sbjct: 433 Q*LS*CF*YTWMVLWT*FLFN 495
Score = 22.7 bits (47), Expect(2) = 7e-04
Identities = 8/13 (61%), Positives = 13/13 (99%)
Frame = +2
Query: 205 LLLTRVRMFLNPG 217
+LL+R+R+FL+PG
Sbjct: 218 VLLSRLRLFLSPG 256
>TC15878 homologue to UP|Q8L6S6 (Q8L6S6) Endo-beta-1,4-glucanase , partial
(24%)
Length = 786
Score = 35.4 bits (80), Expect = 0.015
Identities = 25/67 (37%), Positives = 32/67 (47%), Gaps = 5/67 (7%)
Frame = +3
Query: 52 DHARELIKWGTDYLLLTFDSSASEINQIHAKVGGSLKGS---EKPEDMEYPRPTTTI*SE 108
DHA E +KWGTDY F + E N ++ +VG ++PEDM R I
Sbjct: 402 DHAMEAVKWGTDY----FIKAHPEPNVLYGEVGDGNTDHYCWQRPEDMTTDRRAYRIDPS 569
Query: 109 GP--DLA 113
P DLA
Sbjct: 570 NPGSDLA 590
>AV780838
Length = 578
Score = 32.7 bits (73), Expect = 0.098
Identities = 19/46 (41%), Positives = 26/46 (56%), Gaps = 3/46 (6%)
Frame = +2
Query: 58 IKWGTDYLLLTFDSSASEINQIHAKVG---GSLKGSEKPEDMEYPR 100
++W TDYLL T S+ N+I +VG G E+PEDM+ R
Sbjct: 395 VRWATDYLLKT----VSQPNRILVQVGDPSSDHDGRERPEDMDTAR 520
>TC16159 homologue to UP|Q94A23 (Q94A23) AT5g64310/MSJ1_15, partial (19%)
Length = 605
Score = 30.4 bits (67), Expect = 0.49
Identities = 12/26 (46%), Positives = 19/26 (72%)
Frame = +3
Query: 181 QWQPILLCLRMPKHLIRLPILISYLL 206
+W+ +LL LR P L+RLP+++ LL
Sbjct: 240 RWRAVLLLLRPPSTLLRLPLMLLLLL 317
>AU088935
Length = 683
Score = 29.3 bits (64), Expect = 1.1
Identities = 23/67 (34%), Positives = 29/67 (42%), Gaps = 5/67 (7%)
Frame = +2
Query: 52 DHARELIKWGTDYLLLTFDSSASEINQIHAKVGGSLKGS---EKPEDMEYPRPTTTI*SE 108
+H E IKWGTDY + S N + A+VG ++ EDM R I
Sbjct: 215 EHTMEAIKWGTDYFIKAHTSP----NVLWAEVGDGDTDHYCWQRAEDMTTSRQAFKIDES 382
Query: 109 GP--DLA 113
P DLA
Sbjct: 383 NPGSDLA 403
>TC16264 similar to GB|AAP21178.1|30102520|BT006370 At3g08040/T8G24.8
{Arabidopsis thaliana;}, partial (8%)
Length = 519
Score = 28.1 bits (61), Expect = 2.4
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = +1
Query: 185 ILLCLRMPKHLIRLPILISYLLLTRVRMFLNPG 217
ILL L P L P+L+ YL T V++ L G
Sbjct: 10 ILLILHTPWSLCH*PVLLPYLFFTGVKVLLGSG 108
>TC8646 similar to UP|Q941A9 (Q941A9) At1g26300/F28B23_4, partial (14%)
Length = 608
Score = 28.1 bits (61), Expect = 2.4
Identities = 16/48 (33%), Positives = 25/48 (51%)
Frame = -2
Query: 315 NNNSYTEPTLAGNAGLVAALISLTSTSTIGSGFDTDHTIFNQTRQYRP 362
N + +T+P+ +GN G V L TS + I + TD+T N+ P
Sbjct: 598 NTSIHTKPSSSGNKGRVFMLALFTS*NFICKNWVTDNTSTNKVNCTSP 455
>TC16038 similar to UP|Q9LXT8 (Q9LXT8) Pre-mRNA splicing factor
ATP-dependent RNA helicase-like protein, partial (6%)
Length = 666
Score = 27.7 bits (60), Expect = 3.1
Identities = 24/78 (30%), Positives = 36/78 (45%), Gaps = 3/78 (3%)
Frame = -1
Query: 255 YTIEPWIPKFSPICC*C-FIYGFSFC*LY-ESPRCSWMVL*S*LLFNI-CSKGICNFSVY 311
Y EP+ ICC*C I+ FSFC L S C++ F+I CS + + +
Sbjct: 297 YVEEPFRKPGMEICC*C*AIFSFSFCFLSGSSSACTF------FKFSISCSMAVFSRFCF 136
Query: 312 DLRNNNSYTEPTLAGNAG 329
L ++N ++ N G
Sbjct: 135 FLCSSNDVSDSLTEKNMG 82
>TC18552 similar to UP|Q9FIM2 (Q9FIM2) Cell division protein FtsH, partial
(10%)
Length = 643
Score = 27.7 bits (60), Expect = 3.1
Identities = 20/66 (30%), Positives = 33/66 (49%), Gaps = 2/66 (3%)
Frame = -2
Query: 180 WQWQPILLCLRMPKHLIRLPILISYLLLTRVRMFLNPG*WLPLRGNFEYVP--*IY*PYH 237
WQWQPI+ + L++L I+I + + R PG +PL G+ P + PY
Sbjct: 258 WQWQPIIQAQEIGALLLQLGIVIFVMRVIR------PG--IPLPGSEPLAPTTVVSVPYS 103
Query: 238 VLLTKI 243
L+++
Sbjct: 102 EFLSRV 85
>TC9870 weakly similar to UP|NO16_MEDTR (P93328) Early nodulin 16 precursor
(N-16), partial (31%)
Length = 581
Score = 26.6 bits (57), Expect = 7.0
Identities = 12/31 (38%), Positives = 15/31 (47%)
Frame = -2
Query: 171 PLVTTPTFPWQWQPILLCLRMPKHLIRLPIL 201
P T P+ P P+ L P H +R PIL
Sbjct: 118 PCYTLPSIPHGLPPLPCTLNSPPHSLRAPIL 26
>TC17342 similar to PIR|JC7226|JC7226 endo-1,3(4)-beta-glucanase - garden
pea {Pisum sativum;} , partial (24%)
Length = 499
Score = 26.6 bits (57), Expect = 7.0
Identities = 10/14 (71%), Positives = 12/14 (85%)
Frame = +2
Query: 53 HARELIKWGTDYLL 66
+ARE I+W TDYLL
Sbjct: 410 NAREAIRWATDYLL 451
>CB826805
Length = 635
Score = 26.2 bits (56), Expect = 9.2
Identities = 10/19 (52%), Positives = 13/19 (67%)
Frame = -2
Query: 243 IVVAIIKICRGIYTIEPWI 261
I+ II+IC I +EPWI
Sbjct: 178 IIFLIIRICWSIVLLEPWI 122
>TC17787
Length = 387
Score = 26.2 bits (56), Expect = 9.2
Identities = 12/20 (60%), Positives = 15/20 (75%)
Frame = +1
Query: 273 IYGFSFC*LYESPRCSWMVL 292
I+GF FC L + RCSWM+L
Sbjct: 103 IWGF-FCFLSQLNRCSWMLL 159
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.343 0.153 0.541
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,493,693
Number of Sequences: 28460
Number of extensions: 128795
Number of successful extensions: 1225
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 1216
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1222
length of query: 367
length of database: 4,897,600
effective HSP length: 92
effective length of query: 275
effective length of database: 2,279,280
effective search space: 626802000
effective search space used: 626802000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.5 bits)
S2: 56 (26.2 bits)
Medicago: description of AC127169.10