BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC127021.6 + phase: 0 /pseudo
(408 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV767121 73 1e-13
AV428268 65 2e-11
AV411435 50 7e-07
BP041113 45 2e-05
AV780705 44 6e-05
AV780221 43 1e-04
AV780250 43 1e-04
AU089037 42 2e-04
CN825238 40 5e-04
CB826728 32 0.013
AV426428 35 0.030
TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%) 34 0.051
TC19933 33 0.066
AV412895 33 0.066
AV426437 31 0.33
BP060613 30 0.95
TC10037 29 1.2
AV775981 29 1.2
AV418280 29 1.6
TC9099 similar to GB|AAO42817.1|28416573|BT004571 At1g27330 {Ara... 29 1.6
>AV767121
Length = 525
Score = 72.8 bits (177), Expect = 1e-13
Identities = 44/139 (31%), Positives = 71/139 (50%)
Frame = +2
Query: 7 LSTWHGRLLSIMGRAQLVNYVISGMLVYTFHIHRWPKIILNEITKIIRNFVWSGDCNQQK 66
LS W + +S GR L+ V++ + ++ + P + +++RNF+W G N+ K
Sbjct: 2 LSKWKQKTVSFGGRIFLIQSVLTALPLFFLSFFKLPIGVGKSCVRLMRNFLWGGSENENK 181
Query: 67 ICTVAWNTMCRPKDEGGLAIRDPFMVNNASLLHLTRKFLTSQDQWAQLCRHILLSNAKPK 126
I V W +C+PK+ GGL I+D F N A L ++LT D L R ++ A+P
Sbjct: 182 IA*VKWTDVCKPKELGGLGIKDLFTFNKALLGK*RWRYLTEPD---SLWRRVI--EAQP- 343
Query: 127 NHYIHSSIWIGLKSWVDVV 145
+HY S W W D++
Sbjct: 344 DHYSCGSSW-----WNDIL 385
>AV428268
Length = 429
Score = 65.1 bits (157), Expect = 2e-11
Identities = 28/90 (31%), Positives = 48/90 (53%), Gaps = 3/90 (3%)
Frame = +3
Query: 223 KLTYLFVRGTCIKVPWH---KLVWNTYIPPTRVFICWRFIHNRLPTDENLRKRGCTIVSI 279
K Y F+ G C W KL+W+ +P + + W+ + NR+ T NL +RG I ++
Sbjct: 15 KSAYDFLHG-CANAQWDNIFKLLWSVKVPSNAIALSWKVLINRVQTKVNLNRRGVVISNV 191
Query: 280 CCFCKKQLETSDHIFLRCPVTSQIWDWLNQ 309
C C E++DH+ CP+ +IW +++
Sbjct: 192 CPLCSLDEESTDHLLFSCPIV*RIWSKISE 281
>AV411435
Length = 426
Score = 50.1 bits (118), Expect = 7e-07
Identities = 32/122 (26%), Positives = 44/122 (35%), Gaps = 13/122 (10%)
Frame = +3
Query: 196 ILQTTLPIKPVPDALQWLSTTDGHLTNKLTYLFVRGTCIKVP-------------WHKLV 242
I+QT I D L W G + K Y V+ T + WH +
Sbjct: 3 IMQTPFSITGSEDRLFWPFIPSGEYSVKSGYRAVKATQFNLSNLASSSSHPSSFLWHT-I 179
Query: 243 WNTYIPPTRVFICWRFIHNRLPTDENLRKRGCTIVSICCFCKKQLETSDHIFLRCPVTSQ 302
W +P WR +N +P NL++R C C K E +H L C T
Sbjct: 180 WGAQVPKKVRSFLWRAANNAIPVKRNLKRRNMGRDDFCPICNKGPEDINHALLACEWTRA 359
Query: 303 IW 304
+W
Sbjct: 360 VW 365
>BP041113
Length = 503
Score = 45.4 bits (106), Expect = 2e-05
Identities = 26/88 (29%), Positives = 43/88 (48%), Gaps = 5/88 (5%)
Frame = +1
Query: 211 QWLSTTDGHLTNKLTYLFVRGTCIKV--PWHKLVWNTYIPPTRVFICWRFIHNRLPTDEN 268
+WL G T Y F++ + P +W T P + F WR + RLPT +N
Sbjct: 232 KWLWLPGGTYTVNSAYSFLQAPTLADTDPIFATIWRTVAPSVKAF-AWRCLLGRLPTYDN 408
Query: 269 LRKRGCTI---VSICCFCKKQLETSDHI 293
L KR + +++C FC+ ++E+ H+
Sbjct: 409 LIKRQVVVDPAMTVCKFCQGEVESVTHL 492
>AV780705
Length = 524
Score = 43.5 bits (101), Expect = 6e-05
Identities = 30/120 (25%), Positives = 53/120 (44%), Gaps = 2/120 (1%)
Frame = -2
Query: 1 DRIKSKLSTWHGRLLSIMGRAQLVNYVISGMLVYTFHIHRWPKIILNEITKIIRNFVWSG 60
+++K KL W LL+ G+ L+ +I + Y I +PK N + ++ +F W
Sbjct: 424 EKVKEKLEGWKETLLNQAGKEVLIKAIIQAIPSYAMTIVHFPKTFCNSLYALVADFWWKS 245
Query: 61 DCNQQKICTVAWNTM-CRPKDEGGLAIRDPFMVNNASLLHLTRKFLTSQDQ-WAQLCRHI 118
T A T K++GG+ RD N A L + LT+ + W ++ + +
Sbjct: 244 QGEGAVEYTGAVGTK*PLSKEKGGVGFRDLRTQNLAFLARQAWRVLTNPEALWVRVMKSL 65
>AV780221
Length = 461
Score = 42.7 bits (99), Expect = 1e-04
Identities = 28/76 (36%), Positives = 39/76 (50%)
Frame = +3
Query: 65 QKICTVAWNTMCRPKDEGGLAIRDPFMVNNASLLHLTRKFLTSQDQWAQLCRHILLSNAK 124
+KI V W+T+CRPKDEGGL +++ + N A L + L +D I NA
Sbjct: 138 KKIAWVKWSTVCRPKDEGGLGVQNLGLFNKALLGKWRWRMLKERDGLWYKVLLIKYKNAI 317
Query: 125 PKNHYIHSSIWIGLKS 140
P++ SS W L S
Sbjct: 318 PQS---ASSWWNDLHS 356
>AV780250
Length = 494
Score = 42.7 bits (99), Expect = 1e-04
Identities = 29/105 (27%), Positives = 42/105 (39%), Gaps = 8/105 (7%)
Frame = +3
Query: 208 DALQWLSTTDGHLTNKLTYLFVRGTCIK--------VPWHKLVWNTYIPPTRVFICWRFI 259
D L W T++G T+K Y VR + + +VW P + R
Sbjct: 174 DVLAWPHTSNGEYTSKSGYAVVRNKVVHALPSSSTALQVSPVVWKAPTLPRCRELIGRAF 353
Query: 260 HNRLPTDENLRKRGCTIVSICCFCKKQLETSDHIFLRCPVTSQIW 304
LPT LR RG + +C C+ E+ + L PV S+ W
Sbjct: 354 LKILPTRSALRARGMDVDELCPSCETHRESPAQVLLFVPVVSKWW 488
>AU089037
Length = 245
Score = 41.6 bits (96), Expect = 2e-04
Identities = 21/61 (34%), Positives = 30/61 (48%), Gaps = 3/61 (4%)
Frame = -1
Query: 242 VWNTYIPPTRVFICWRFIHNRLPTDENLRKRGCTIVS---ICCFCKKQLETSDHIFLRCP 298
+W P V WR I +R+ T NLR+R S +C C + E+S H+F CP
Sbjct: 191 LWKVPAPSNAVSFAWRLILDRVQTRGNLRRRQVIQQSEEALCPMCSQCEESSSHLFFSCP 12
Query: 299 V 299
+
Sbjct: 11 L 9
>CN825238
Length = 692
Score = 40.4 bits (93), Expect = 5e-04
Identities = 20/58 (34%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
Frame = +3
Query: 237 PWHKLVWNTYIPPTRVFICWRFIHNRLPTDENLRKRGCTIVSICCFCKKQL-ETSDHI 293
PW KL W T +P +R N +P ENL++RG + + C C Q+ E+ H+
Sbjct: 522 PWKKL-WRTPVPNKMKHFAYRVARNIIPCRENLQRRGIAVQNECPTCNHQIPESMSHL 692
>CB826728
Length = 509
Score = 31.6 bits (70), Expect(2) = 0.013
Identities = 25/99 (25%), Positives = 38/99 (38%), Gaps = 13/99 (13%)
Frame = +1
Query: 212 WLSTTDGHLTNKLTYLFVR----------GTCIKVP---WHKLVWNTYIPPTRVFICWRF 258
W ++DG ++K Y F+R VP W K VW+ P + WR
Sbjct: 214 WPGSSDGWYSSKSGYEFIRLENKSLLPSTSPAAAVPSLIW-KTVWSASSLPRCKEVMWRA 390
Query: 259 IHNRLPTDENLRKRGCTIVSICCFCKKQLETSDHIFLRC 297
LP L++RG + + + E H+ L C
Sbjct: 391 CAGYLPVRSALKRRGLDVDPSSPWSGLEDEAEVHVLLTC 507
Score = 23.1 bits (48), Expect(2) = 0.013
Identities = 13/41 (31%), Positives = 18/41 (43%)
Frame = +3
Query: 170 VSDYIVDGEWNLPTYFHVKDDVLTNKILQTTLPIKPVPDAL 210
V+D I +G WN V T L +LP++ D L
Sbjct: 87 VADLIENGRWNENLVSMVFSPSTTAVFLAVSLPLQSYEDCL 209
>AV426428
Length = 413
Score = 34.7 bits (78), Expect = 0.030
Identities = 20/63 (31%), Positives = 24/63 (37%)
Frame = +3
Query: 242 VWNTYIPPTRVFICWRFIHNRLPTDENLRKRGCTIVSICCFCKKQLETSDHIFLRCPVTS 301
+W P WR LP +L RG IC C +T+DH L CP
Sbjct: 54 LWKAEALPRCCETGWRACLGVLPVKASLHARGLAEDPICPRCLAAPKTADHAVLFCP*VH 233
Query: 302 QIW 304
IW
Sbjct: 234 PIW 242
>TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%)
Length = 912
Score = 33.9 bits (76), Expect = 0.051
Identities = 16/31 (51%), Positives = 19/31 (60%)
Frame = -3
Query: 1 DRIKSKLSTWHGRLLSIMGRAQLVNYVISGM 31
DR SKL W GR LS GR QL+N ++ M
Sbjct: 97 DRKPSKLQHWTGRSLSYAGRLQLINSILFSM 5
>TC19933
Length = 472
Score = 33.5 bits (75), Expect = 0.066
Identities = 12/28 (42%), Positives = 15/28 (52%)
Frame = -3
Query: 256 WRFIHNRLPTDENLRKRGCTIVSICCFC 283
W+ HN +P NLR+RGC C C
Sbjct: 92 WKLCHNAIPLKGNLRRRGCATDGKCPIC 9
>AV412895
Length = 405
Score = 33.5 bits (75), Expect = 0.066
Identities = 18/66 (27%), Positives = 27/66 (40%), Gaps = 3/66 (4%)
Frame = +1
Query: 242 VWNTYIPPTRVFICWRFIHNRLPTDENLRKRGC---TIVSICCFCKKQLETSDHIFLRCP 298
+W P WR + R+ T +NL KR + +IC C E+ H+ C
Sbjct: 82 IWMVPAPLNIKAFVWRVLLGRIQTRDNLLKRQVIHNALDAICPLCGLAEESGSHLLFSCA 261
Query: 299 VTSQIW 304
+ IW
Sbjct: 262 ESMLIW 279
>AV426437
Length = 303
Score = 31.2 bits (69), Expect = 0.33
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = +1
Query: 61 DCNQQKICTVAWNTMCRPKDEGGLAIRD 88
D NQ+KI + W T+C+PK GL +++
Sbjct: 211 DENQRKIAWINWETICKPKWLRGLGVKN 294
>BP060613
Length = 378
Score = 29.6 bits (65), Expect = 0.95
Identities = 22/87 (25%), Positives = 38/87 (43%), Gaps = 1/87 (1%)
Frame = +1
Query: 57 VWSGDCNQQKICTVAWNTMCRPKDEGGLAIRDPFMVNNASLLHLTRKFLTSQDQ-WAQLC 115
VW + + W+ + KDEGGL +D + N A L + L + D W Q+
Sbjct: 43 VWWSSKGTRGVHWRKWDLLTELKDEGGLGFKDFEIQNQALLAKQAWRILHNPDALWVQIL 222
Query: 116 RHILLSNAKPKNHYIHSSIWIGLKSWV 142
+ + P + ++ ++ G SWV
Sbjct: 223 KALYF----PHHDFLQTTKRTG-ASWV 288
>TC10037
Length = 560
Score = 29.3 bits (64), Expect = 1.2
Identities = 13/48 (27%), Positives = 24/48 (49%), Gaps = 1/48 (2%)
Frame = -3
Query: 258 FIHNRLPTDENLRKRGCTIVSICCF-CKKQLETSDHIFLRCPVTSQIW 304
++ + + T + L +G + +I C C + ET DH CP ++W
Sbjct: 456 YLRSIVLTRKRLWNKGAVVPTIFCLRCDRLEETMDHALRDCP*ARRVW 313
>AV775981
Length = 304
Score = 29.3 bits (64), Expect = 1.2
Identities = 11/34 (32%), Positives = 16/34 (46%)
Frame = -1
Query: 216 TDGHLTNKLTYLFVRGTCIKVPWHKLVWNTYIPP 249
T ++ N TY+F+ G C P H W+ P
Sbjct: 121 TPSYI*NIYTYIFIYGICYDTPTHHFSWSMIAQP 20
>AV418280
Length = 398
Score = 28.9 bits (63), Expect = 1.6
Identities = 15/45 (33%), Positives = 22/45 (48%), Gaps = 2/45 (4%)
Frame = -2
Query: 280 CCFCKKQLETSDHIFLRCPVTSQIWDWLNQ--GINQQLDLSSCQN 322
C FC E S H+F C + +W L++ GI+ L S+ N
Sbjct: 388 CPFCTTVEECSGHLFFTCVFSMGVWQALHRWLGISVALPASTLAN 254
>TC9099 similar to GB|AAO42817.1|28416573|BT004571 At1g27330 {Arabidopsis
thaliana;}, complete
Length = 518
Score = 28.9 bits (63), Expect = 1.6
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = +1
Query: 269 LRKRGCTIVSICCFCKKQLETSDHIFLRCPVTSQIWDWLN 308
LRK T++++CC L + DH+ R ++ WLN
Sbjct: 241 LRKGKTTLLALCCSVSLSLLSLDHLSFR*SGQQRVEAWLN 360
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.333 0.142 0.502
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,917,909
Number of Sequences: 28460
Number of extensions: 180049
Number of successful extensions: 1409
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 1398
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1406
length of query: 408
length of database: 4,897,600
effective HSP length: 92
effective length of query: 316
effective length of database: 2,279,280
effective search space: 720252480
effective search space used: 720252480
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.5 bits)
S2: 56 (26.2 bits)
Medicago: description of AC127021.6