BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126786.8 - phase: 0 /pseudo
(688 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV780255 114 4e-26
TC17481 66 2e-11
CB827611 56 2e-08
TC20168 54 6e-08
TC17821 similar to UP|O49656 (O49656) PREDICTED protein, partial... 45 5e-05
TC8818 weakly similar to PIR|T48456|T48456 rna binding protein-l... 32 0.44
TC8891 similar to GB|AAP68279.1|31711846|BT008840 At1g72680 {Ara... 31 0.75
BP075231 29 2.2
TC8858 homologue to UP|Q84N37 (Q84N37) Potyvirus VPg interacting... 29 2.2
BP066639 29 2.2
TC12799 weakly similar to UP|Q9FM10 (Q9FM10) Similarity to MtN3 ... 28 4.9
TC13028 similar to UP|Q9C698 (Q9C698) Mysoin-like protein, parti... 28 6.3
TC10768 weakly similar to UP|Q8I8Z5 (Q8I8Z5) Heat shock protein ... 27 8.3
TC8724 homologue to UP|Q9AQU5 (Q9AQU5) Plasma membrane integral ... 27 8.3
>AV780255
Length = 550
Score = 114 bits (286), Expect = 4e-26
Identities = 58/111 (52%), Positives = 73/111 (65%)
Frame = +3
Query: 1 MGRRHGWELPFHTFQVVAITVFFLLCIAYYAFFAPFLGKDIFEFVAFGVYSLLALSVFFL 60
M R+HGW+LP HTFQVVAITVF LL IA+YAF APF+G I+E+ GVYS +AL+VF L
Sbjct: 72 MVRKHGWQLPAHTFQVVAITVFCLLVIAFYAFLAPFIGGHIWEYTFIGVYSPVALTVFIL 251
Query: 61 YVRCTAIDPADLGVMIDCDKTSKNRSKLDEELAEPSKTRLKDDGISDQHDS 111
YVRCTAI+PAD G+M D N+ L ++ D + +H S
Sbjct: 252 YVRCTAINPADPGIMSKFDPRVGNKFNSSHGLLGKHQSSEHDGNTAGEHSS 404
>TC17481
Length = 500
Score = 65.9 bits (159), Expect = 2e-11
Identities = 36/52 (69%), Positives = 43/52 (82%), Gaps = 1/52 (1%)
Frame = +2
Query: 638 *VNSQMQEQEIVAQ*L-VFRTEIIL*GIFSKVDHIEVPSFLCSFPVIPSKTS 688
*+N+Q++EQEI A L VFR EI+ GIFS+VDHIEVPSFLC F VIPS+TS
Sbjct: 11 *MNNQLREQEIAAPYLLVFRIEILCQGIFSQVDHIEVPSFLCLFLVIPSQTS 166
>CB827611
Length = 559
Score = 56.2 bits (134), Expect = 2e-08
Identities = 29/84 (34%), Positives = 38/84 (44%)
Frame = +3
Query: 149 FCTLCNAEVRKFSKHCRSCDKCVDGFDHHCRWLNNCVGRKNYISFVCLMAVSLVWVSCMK 208
+C C HC CD CV+ FDHHC W+ C+G +NY F + VS + C+
Sbjct: 96 YCDTCMLYRPPRCSHCSICDNCVERFDHHCPWVGQCIGLRNYRYF--FLFVSSATILCIY 269
Query: 209 MPISLS*I*LSLSPFYSSVLY*KN 232
+ S S FY VL N
Sbjct: 270 V--------FSFSAFYIKVLMDNN 317
>TC20168
Length = 937
Score = 54.3 bits (129), Expect = 6e-08
Identities = 22/65 (33%), Positives = 31/65 (46%), Gaps = 9/65 (13%)
Frame = +2
Query: 149 FCTLCNAEVRKFSKHCRSCDKCVDGFDHHCRWLNNCVGRKNYISF---------VCLMAV 199
+C C HC C+ CV+ FDHHC W+ C+G +NY F +C+
Sbjct: 554 YCDTCMLYRPPRCSHCSICNNCVERFDHHCPWVGQCIGLRNYRFFFMFVFSTTLLCIYVF 733
Query: 200 SLVWV 204
+L WV
Sbjct: 734 ALCWV 748
>TC17821 similar to UP|O49656 (O49656) PREDICTED protein, partial (16%)
Length = 535
Score = 44.7 bits (104), Expect = 5e-05
Identities = 19/49 (38%), Positives = 23/49 (46%)
Frame = +1
Query: 138 LQQQSGEEDALFCTLCNAEVRKFSKHCRSCDKCVDGFDHHCRWLNNCVG 186
+Q S E +C C HC C +CV DHHC W+ NCVG
Sbjct: 382 VQSDSLNERTRYCRKCTQLKPPRCHHCSVCGRCVLKMDHHCVWVVNCVG 528
>TC8818 weakly similar to PIR|T48456|T48456 rna binding protein-like -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(90%)
Length = 1079
Score = 31.6 bits (70), Expect = 0.44
Identities = 12/22 (54%), Positives = 17/22 (76%)
Frame = -2
Query: 16 VVAITVFFLLCIAYYAFFAPFL 37
+ A+T+F L CIA++AFF P L
Sbjct: 127 LAAVTLFKLFCIAFFAFFPPIL 62
>TC8891 similar to GB|AAP68279.1|31711846|BT008840 At1g72680 {Arabidopsis
thaliana;} , complete
Length = 1636
Score = 30.8 bits (68), Expect = 0.75
Identities = 10/22 (45%), Positives = 13/22 (58%)
Frame = +1
Query: 157 VRKFSKHCRSCDKCVDGFDHHC 178
V + CR C+ C DG +HHC
Sbjct: 370 VGTYVNSCRDCEFCDDGLEHHC 435
>BP075231
Length = 456
Score = 29.3 bits (64), Expect = 2.2
Identities = 17/46 (36%), Positives = 21/46 (44%)
Frame = +3
Query: 125 LAREDCRSDEDFILQQQSGEEDALFCTLCNAEVRKFSKHCRSCDKC 170
LA E C L + S EDA+FC NA + F +S D C
Sbjct: 240 LANESCG------LGRPSAWEDAVFCIFSNANIGSFLDTSKSHDSC 359
>TC8858 homologue to UP|Q84N37 (Q84N37) Potyvirus VPg interacting protein
(Fragment), partial (65%)
Length = 1272
Score = 29.3 bits (64), Expect = 2.2
Identities = 19/65 (29%), Positives = 27/65 (41%), Gaps = 12/65 (18%)
Frame = +1
Query: 112 NGCSKLGCCLCSFLAREDCRSDEDFI-----LQQQSGEEDALF-CTLCNAE------VRK 159
N C +GC LCS DC E I ++ +G + +F C CN V+
Sbjct: 25 NTCRWIGCDLCSHWTHTDCAIREQLICMGPTVKSGAGPSEMVFRCQACNRTSELLGWVKD 204
Query: 160 FSKHC 164
+HC
Sbjct: 205 VFQHC 219
>BP066639
Length = 291
Score = 29.3 bits (64), Expect = 2.2
Identities = 17/52 (32%), Positives = 25/52 (47%), Gaps = 7/52 (13%)
Frame = -3
Query: 76 IDCDKTSKNRSKLDEELAEPSKTRLK------DDGISDQHDSNGC-SKLGCC 120
+D D ++ + D L E +T+L+ DDG+ D NGC K CC
Sbjct: 196 VDEDLSTDGSAVADSSLNETQETKLEKSSIIQDDGLMDYQGCNGCHCKFICC 41
>TC12799 weakly similar to UP|Q9FM10 (Q9FM10) Similarity to MtN3 protein,
partial (35%)
Length = 806
Score = 28.1 bits (61), Expect = 4.9
Identities = 18/48 (37%), Positives = 23/48 (47%)
Frame = +1
Query: 14 FQVVAITVFFLLCIAYYAFFAPFLGKDIFEFVAFGVYSLLALSVFFLY 61
F +++F LC + F LG+D F FV GV SLL LY
Sbjct: 355 FMPFFLSLFVFLCGTSWFIFG-LLGRDPFLFVPNGVGSLLGTMQLILY 495
>TC13028 similar to UP|Q9C698 (Q9C698) Mysoin-like protein, partial (10%)
Length = 605
Score = 27.7 bits (60), Expect = 6.3
Identities = 22/78 (28%), Positives = 33/78 (42%), Gaps = 6/78 (7%)
Frame = +3
Query: 143 GEEDALFCTLCNAEVRKFSKHCRS------CDKCVDGFDHHCRWLNNCVGRKNYISFVCL 196
GEED TLC+ HC C + + G + RW+ GRKN + +CL
Sbjct: 87 GEEDLTNFTLCDLPFNIPIHHCCRHHLLCFCREILHGL-YSFRWIGAGHGRKNRLIRLCL 263
Query: 197 MAVSLVWVSCMKMPISLS 214
W +K+P ++
Sbjct: 264 RK*LWHWTP-LKLPTRIA 314
>TC10768 weakly similar to UP|Q8I8Z5 (Q8I8Z5) Heat shock protein 90
(Fragment), partial (4%)
Length = 560
Score = 27.3 bits (59), Expect = 8.3
Identities = 13/42 (30%), Positives = 25/42 (58%)
Frame = +2
Query: 19 ITVFFLLCIAYYAFFAPFLGKDIFEFVAFGVYSLLALSVFFL 60
+++FF+L I+Y+A+ +F V+ ++A+S FFL
Sbjct: 314 LSIFFVLLISYFAYTN*VETGILFARATLSVFFVVAISHFFL 439
>TC8724 homologue to UP|Q9AQU5 (Q9AQU5) Plasma membrane integral protein
ZmPIP1-4 (Plasma membrane integral protein ZmPIP1-3),
partial (93%)
Length = 1560
Score = 27.3 bits (59), Expect = 8.3
Identities = 11/33 (33%), Positives = 19/33 (57%)
Frame = -1
Query: 174 FDHHCRWLNNCVGRKNYISFVCLMAVSLVWVSC 206
F +HC++ N Y+ +CL+ +SL +SC
Sbjct: 1341 FSNHCQYTN-------YLKLICLIKISLRLLSC 1264
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.354 0.157 0.536
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,778,050
Number of Sequences: 28460
Number of extensions: 220133
Number of successful extensions: 2915
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 2123
Number of HSP's successfully gapped in prelim test: 89
Number of HSP's that attempted gapping in prelim test: 743
Number of HSP's gapped (non-prelim): 2282
length of query: 688
length of database: 4,897,600
effective HSP length: 97
effective length of query: 591
effective length of database: 2,136,980
effective search space: 1262955180
effective search space used: 1262955180
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC126786.8