Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC126786.2 - phase: 0 
         (161 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BP040442                                                               74  9e-15
BP069452                                                               42  4e-05
BI418308                                                               33  0.018
TC19822 similar to UP|EX13_ARATH (Q9M9P0) Alpha-expansin 13 prec...    28  0.58
TC14269 weakly similar to UP|Q13484 (Q13484) Ankyrin G119, parti...    28  0.99
BP043528                                                               27  1.3
BF177830                                                               27  1.7
TC13193 similar to UP|Q9ASQ6 (Q9ASQ6) At1g29720/T3M22_6, partial...    26  2.9
AV418608                                                               26  2.9
TC14104 GB|CAA61590.1|897773|LJAS2GENE asparagine synthase (glut...    26  3.8
BP078464                                                               25  4.9
TC8711 weakly similar to UP|VCLC_PEA (P13918) Vicilin precursor,...    25  4.9
TC11470                                                                25  8.4
TC7990 similar to UP|Q7X9B3 (Q7X9B3) 9/13 hydroperoxide lyase, p...    25  8.4

>BP040442 
          Length = 546

 Score = 74.3 bits (181), Expect = 9e-15
 Identities = 35/42 (83%), Positives = 38/42 (90%)
 Frame = +1

Query: 1   ISRNLKYEDPFDNPLVKVSKSKSSVEMCGKVYRLAPVTLTQE 42
           ISR LKYEDPF+NP+V V KS S+VEMCGKVYRLAPVTLTQE
Sbjct: 421 ISRPLKYEDPFNNPMVXVGKSNSTVEMCGKVYRLAPVTLTQE 546


>BP069452 
          Length = 491

 Score = 42.4 bits (98), Expect = 4e-05
 Identities = 20/21 (95%), Positives = 20/21 (95%)
 Frame = -3

Query: 110 VPFRIQAEHEALQRKLEALQN 130
           VPFRIQAEHEALQRKLEAL N
Sbjct: 489 VPFRIQAEHEALQRKLEALPN 427


>BI418308 
          Length = 411

 Score = 33.5 bits (75), Expect = 0.018
 Identities = 22/66 (33%), Positives = 35/66 (52%), Gaps = 1/66 (1%)
 Frame = +2

Query: 62  VFLKEGESVPPDVDPDTIRWIPANHPFATTSTDIGEDFAHKNVSQKHGVPFRIQAEHEAL 121
           V ++  +  PP   PDT   I ANHPF      IG+  A  ++ ++ G PFR+    + L
Sbjct: 35  VRIRTADQWPPAPLPDTPLVIVANHPFG-----IGDGIAVLSLVEQLGRPFRVMIHKDLL 199

Query: 122 Q-RKLE 126
           + R++E
Sbjct: 200 KIREME 217


>TC19822 similar to UP|EX13_ARATH (Q9M9P0) Alpha-expansin 13 precursor
           (At-EXP13) (AtEx13) (Ath-ExpAlpha-1.22), partial (27%)
          Length = 532

 Score = 28.5 bits (62), Expect = 0.58
 Identities = 13/30 (43%), Positives = 16/30 (53%), Gaps = 2/30 (6%)
 Frame = -1

Query: 68  ESVPPDVDPDTIRWIP--ANHPFATTSTDI 95
           ES PP+  P    W+P  A H  ATTS  +
Sbjct: 394 ESTPPEQPPSAAEWLPATATHYTATTSDSV 305


>TC14269 weakly similar to UP|Q13484 (Q13484) Ankyrin G119, partial (3%)
          Length = 656

 Score = 27.7 bits (60), Expect = 0.99
 Identities = 14/49 (28%), Positives = 25/49 (50%), Gaps = 3/49 (6%)
 Frame = -3

Query: 45  AVHQRRRSRAY---QWKRPTVFLKEGESVPPDVDPDTIRWIPANHPFAT 90
           AV +RR   A    +W+  ++  +E  S+ P +    ++W PA  PF +
Sbjct: 303 AVMKRRIKSAIIDVRWQESSILYQELHSILPPLFGCNVKWSPATSPFCS 157


>BP043528 
          Length = 518

 Score = 27.3 bits (59), Expect = 1.3
 Identities = 11/27 (40%), Positives = 15/27 (54%)
 Frame = -2

Query: 69  SVPPDVDPDTIRWIPANHPFATTSTDI 95
           S   D  P  I+W P N  FAT S+++
Sbjct: 463 SATSDTGPPVIKWAPGNLMFATGSSEL 383


>BF177830 
          Length = 476

 Score = 26.9 bits (58), Expect = 1.7
 Identities = 12/37 (32%), Positives = 20/37 (53%)
 Frame = +2

Query: 20  KSKSSVEMCGKVYRLAPVTLTQEEQAVHQRRRSRAYQ 56
           K     ++CG+  +  PVTL +   +V Q R+S+  Q
Sbjct: 347 KGNKKCDVCGQEVQNLPVTLLRVSSSVRQNRQSQNQQ 457


>TC13193 similar to UP|Q9ASQ6 (Q9ASQ6) At1g29720/T3M22_6, partial (12%)
          Length = 513

 Score = 26.2 bits (56), Expect = 2.9
 Identities = 23/77 (29%), Positives = 29/77 (36%), Gaps = 18/77 (23%)
 Frame = +1

Query: 59  RPT----VFLKEGESVPPDVDPDT--------------IRWIPANHPFATTSTDIGEDFA 100
           RPT    V + EG    PDVDP+T              IR    NH  +T+ TD      
Sbjct: 103 RPTMSEVVNMLEGRISIPDVDPETSVFREDLRFKAMRDIRQHKENHSLSTSQTDNSTGLT 282

Query: 101 HKNVSQKHGVPFRIQAE 117
           H   S       +I +E
Sbjct: 283 HSFPSTSGNDMHQISSE 333


>AV418608 
          Length = 223

 Score = 26.2 bits (56), Expect = 2.9
 Identities = 9/30 (30%), Positives = 15/30 (50%)
 Frame = -2

Query: 56 QWKRPTVFLKEGESVPPDVDPDTIRWIPAN 85
          QWK P +  +E +S    +D +   W+  N
Sbjct: 93 QWKVPRIMKRESKSKEESIDLEVWNWLEGN 4


>TC14104 GB|CAA61590.1|897773|LJAS2GENE asparagine synthase
           (glutamine-hydrolysing) {Lotus corniculatus var.
           japonicus;} , complete
          Length = 2259

 Score = 25.8 bits (55), Expect = 3.8
 Identities = 11/40 (27%), Positives = 21/40 (52%)
 Frame = -2

Query: 2   SRNLKYEDPFDNPLVKVSKSKSSVEMCGKVYRLAPVTLTQ 41
           +R LK  +PF   +++ +K       C K+++L    LT+
Sbjct: 230 ARKLKDANPFGLRVIRTTKHSKYATHCDKIFKLLKPKLTK 111


>BP078464 
          Length = 390

 Score = 25.4 bits (54), Expect = 4.9
 Identities = 10/32 (31%), Positives = 17/32 (52%)
 Frame = -1

Query: 30  KVYRLAPVTLTQEEQAVHQRRRSRAYQWKRPT 61
           K+YR   +++ Q  Q +HQ+       W +PT
Sbjct: 261 KLYRWISLSICQGRQLLHQKTGVHVSWWLKPT 166


>TC8711 weakly similar to UP|VCLC_PEA (P13918) Vicilin precursor, partial
            (74%)
          Length = 1591

 Score = 25.4 bits (54), Expect = 4.9
 Identities = 13/46 (28%), Positives = 24/46 (51%)
 Frame = +2

Query: 48   QRRRSRAYQWKRPTVFLKEGESVPPDVDPDTIRWIPANHPFATTST 93
            QR +    + ++P +   E +     + P  +  IPA+HPFA T++
Sbjct: 1331 QRGQEEEQEEEQPRI---EAQRFRARLSPGDVIVIPASHPFAVTAS 1459


>TC11470 
          Length = 505

 Score = 24.6 bits (52), Expect = 8.4
 Identities = 13/46 (28%), Positives = 26/46 (56%)
 Frame = +3

Query: 61  TVFLKEGESVPPDVDPDTIRWIPANHPFATTSTDIGEDFAHKNVSQ 106
           T+ LK+ ++   D+D ++++W P   P   +++D G     + VSQ
Sbjct: 132 TLLLKKEKA--HDMDINSVQWSPGEKPLLASASDDGTIKVWELVSQ 263


>TC7990 similar to UP|Q7X9B3 (Q7X9B3) 9/13 hydroperoxide lyase, partial
           (24%)
          Length = 599

 Score = 24.6 bits (52), Expect = 8.4
 Identities = 10/21 (47%), Positives = 12/21 (56%), Gaps = 3/21 (14%)
 Frame = -2

Query: 69  SVPPDVDPDTI---RWIPANH 86
           S PPD  PDT+    W P +H
Sbjct: 232 SAPPDSSPDTVYYQPWAPPSH 170


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.318    0.133    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,812,338
Number of Sequences: 28460
Number of extensions: 35600
Number of successful extensions: 192
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of query: 161
length of database: 4,897,600
effective HSP length: 83
effective length of query: 78
effective length of database: 2,535,420
effective search space: 197762760
effective search space used: 197762760
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)


Medicago: description of AC126786.2