BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126783.2 + phase: 0
(147 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9313 homologue to UP|O82529 (O82529) Ribosomal protein L27a, p... 267 5e-73
TC16050 similar to UP|O82529 (O82529) Ribosomal protein L27a, pa... 265 2e-72
BI420812 187 5e-49
TC18120 similar to UP|O82529 (O82529) Ribosomal protein L27a, pa... 47 1e-06
AW428812 28 0.84
AV424892 28 0.84
AV765320 27 1.1
TC17596 similar to UP|Q42768 (Q42768) Acetohydroxyacid synthase ... 27 1.9
BP043829 27 1.9
TC8266 similar to UP|Q9SKN4 (Q9SKN4) At2g28360 protein, partial ... 27 1.9
AV425423 27 1.9
TC15644 weakly similar to UP|O80606 (O80606) At2g02870 protein (... 26 2.4
TC15237 similar to UP|AAQ24632 (AAQ24632) GPRP, partial (64%) 26 2.4
TC18321 similar to UP|ORN_ARATH (Q9ZVE0) Probable oligoribonucle... 26 3.2
AV779100 25 4.2
BP072692 25 4.2
BP054828 25 5.4
TC9459 similar to AAQ23982 (AAQ23982) Transcription factor Rap21... 25 5.4
AV413463 25 7.1
TC14567 homologue to PIR|T09261|T09261 JUN kinase-activation-dom... 25 7.1
>TC9313 homologue to UP|O82529 (O82529) Ribosomal protein L27a, partial
(94%)
Length = 624
Score = 267 bits (683), Expect = 5e-73
Identities = 127/147 (86%), Positives = 139/147 (94%)
Frame = +3
Query: 1 MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60
MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY
Sbjct: 39 MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 218
Query: 61 FHKLRNKFYNPIVNIDKIASLIPQDVKEKALKENKAPVIDVTQFGFFKLLGKGVLPQNQP 120
FHKLRN+F+ PIVNI+K+ SL+PQDVK+KA K+ APV+DVTQ+G+FK+LGKGVLP NQP
Sbjct: 219 FHKLRNQFHCPIVNIEKLWSLLPQDVKDKASKD-AAPVVDVTQYGYFKVLGKGVLPSNQP 395
Query: 121 FVVKTKLISKIAEKKIKEAGGAVVLTA 147
VVK KL+SKIAEKKIKEAGGAVVLTA
Sbjct: 396 LVVKAKLVSKIAEKKIKEAGGAVVLTA 476
>TC16050 similar to UP|O82529 (O82529) Ribosomal protein L27a, partial (97%)
Length = 634
Score = 265 bits (677), Expect = 2e-72
Identities = 125/148 (84%), Positives = 139/148 (93%), Gaps = 1/148 (0%)
Frame = +3
Query: 1 MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60
MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY
Sbjct: 51 MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 230
Query: 61 FHKLRNKFYNPIVNIDKIASLIPQDVKEKALK-ENKAPVIDVTQFGFFKLLGKGVLPQNQ 119
FHKLRN+F+ PIVN++K+ SL+PQ+ K+KA K + APV+DVTQ+GFFK+LGKGVLP+NQ
Sbjct: 231 FHKLRNQFHCPIVNVEKLWSLLPQEAKDKAAKSKGTAPVVDVTQYGFFKVLGKGVLPENQ 410
Query: 120 PFVVKTKLISKIAEKKIKEAGGAVVLTA 147
P VVK KLISKIAEKKIKEAGGAV+LTA
Sbjct: 411 PLVVKAKLISKIAEKKIKEAGGAVLLTA 494
>BI420812
Length = 490
Score = 187 bits (476), Expect = 5e-49
Identities = 86/151 (56%), Positives = 112/151 (73%), Gaps = 4/151 (2%)
Frame = +1
Query: 1 MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60
M +R KNRKKRG+VS GHGR+GKHRKHPGGRGNAGG+HHHRI FDKYHPGYFGKVG+R+
Sbjct: 4 MPSRLGKNRKKRGNVSGGHGRVGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGIRH 183
Query: 61 FHKLRNKFYNPIVNIDKIASLIPQDVKEKA----LKENKAPVIDVTQFGFFKLLGKGVLP 116
+H RN + P++NID + L+ ++ ++K L E PVIDVT++G+ K+LGKG +P
Sbjct: 184 YHLKRNTEFCPVINIDHLWGLLSEEERDKVFNKQLPEGHVPVIDVTRYGYHKVLGKGKMP 363
Query: 117 QNQPFVVKTKLISKIAEKKIKEAGGAVVLTA 147
+ P +VK K ++ AEK IK GG +L A
Sbjct: 364 EGFPLIVKAKFFTEKAEKCIKAVGGVCLLVA 456
>TC18120 similar to UP|O82529 (O82529) Ribosomal protein L27a, partial (19%)
Length = 390
Score = 47.4 bits (111), Expect = 1e-06
Identities = 21/28 (75%), Positives = 22/28 (78%)
Frame = +3
Query: 1 MTTRFKKNRKKRGHVSAGHGRIGKHRKH 28
MTT FKKN KKR HVS GHGRI K +KH
Sbjct: 297 MTTHFKKNMKKRSHVSTGHGRIEKLKKH 380
>AW428812
Length = 472
Score = 27.7 bits (60), Expect = 0.84
Identities = 14/42 (33%), Positives = 25/42 (59%)
Frame = +2
Query: 37 GMHHHRILFDKYHPGYFGKVGMRYFHKLRNKFYNPIVNIDKI 78
G +H IL D++H ++GM+ LRN+F N + N+ ++
Sbjct: 59 GTAYHGILDDRHHV-MVKRLGMKTCPALRNRFSNELRNLARL 181
>AV424892
Length = 190
Score = 27.7 bits (60), Expect = 0.84
Identities = 12/31 (38%), Positives = 18/31 (57%)
Frame = -1
Query: 6 KKNRKKRGHVSAGHGRIGKHRKHPGGRGNAG 36
++ R++ GH +AG G G HR+ GG G
Sbjct: 100 RRRRRRHGHGAAGQGGAGAHRE--GGAARKG 14
>AV765320
Length = 572
Score = 27.3 bits (59), Expect = 1.1
Identities = 18/52 (34%), Positives = 23/52 (43%), Gaps = 2/52 (3%)
Frame = -3
Query: 19 HGRIGKHRKHPGGRGNAGGMHHH--RILFDKYHPGYFGKVGMRYFHKLRNKF 68
HG + +H +HPGG HHH R L G+ G+ Y H R F
Sbjct: 159 HGHV-RHCQHPGGGNGQWRDHHHWWRRLGGGGGEGWSGREDEPYLHGWRCVF 7
>TC17596 similar to UP|Q42768 (Q42768) Acetohydroxyacid synthase , partial
(16%)
Length = 537
Score = 26.6 bits (57), Expect = 1.9
Identities = 11/35 (31%), Positives = 20/35 (56%)
Frame = +2
Query: 2 TTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAG 36
+T +++ R++ H+S + R+HPGG AG
Sbjct: 197 STTYRRPRRRDLHLSLPRR*ATQRRRHPGGGSGAG 301
>BP043829
Length = 497
Score = 26.6 bits (57), Expect = 1.9
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = +3
Query: 6 KKNRKKRGHVSAGHGRIGKHRKH 28
+ +R ++GH GHG +HR H
Sbjct: 378 RSHRHRQGHGGGGHGHGHRHRHH 446
>TC8266 similar to UP|Q9SKN4 (Q9SKN4) At2g28360 protein, partial (9%)
Length = 764
Score = 26.6 bits (57), Expect = 1.9
Identities = 18/67 (26%), Positives = 36/67 (52%)
Frame = +1
Query: 78 IASLIPQDVKEKALKENKAPVIDVTQFGFFKLLGKGVLPQNQPFVVKTKLISKIAEKKIK 137
+ SL +DV+ ++ ++ T+ + L +G++ + P +K ++ K+AEK+
Sbjct: 100 VPSLFEEDVEFVGVE------LEGTEKAMDQALKEGIVGEAGP--LKRNMVPKVAEKENS 255
Query: 138 EAGGAVV 144
E GGA V
Sbjct: 256 EEGGAGV 276
>AV425423
Length = 334
Score = 26.6 bits (57), Expect = 1.9
Identities = 17/46 (36%), Positives = 20/46 (42%)
Frame = -3
Query: 12 RGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVG 57
RG G + R+H GGRG A G DK H G+VG
Sbjct: 272 RGGQRQGSRECRRPRRHCGGRGGAAGGREGNSRADKGH----GEVG 147
>TC15644 weakly similar to UP|O80606 (O80606) At2g02870 protein
(At2g02870/T17M13.4), partial (10%)
Length = 683
Score = 26.2 bits (56), Expect = 2.4
Identities = 10/25 (40%), Positives = 13/25 (52%)
Frame = -3
Query: 22 IGKHRKHPGGRGNAGGMHHHRILFD 46
+GK KH GNA HH I+ +
Sbjct: 207 LGKFTKHYSPNGNASASHHRTIIHE 133
>TC15237 similar to UP|AAQ24632 (AAQ24632) GPRP, partial (64%)
Length = 814
Score = 26.2 bits (56), Expect = 2.4
Identities = 16/45 (35%), Positives = 20/45 (43%)
Frame = +2
Query: 13 GHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVG 57
GH+ GHG+ +H K G+ G H H G FGK G
Sbjct: 512 GHM--GHGKFKQHGKFKHGKHGKFGKHKH---------GKFGKHG 613
>TC18321 similar to UP|ORN_ARATH (Q9ZVE0) Probable oligoribonuclease ,
partial (78%)
Length = 846
Score = 25.8 bits (55), Expect = 3.2
Identities = 13/46 (28%), Positives = 25/46 (54%), Gaps = 4/46 (8%)
Frame = +1
Query: 59 RYFHKLRNKFYNPIVNIDKIASLI----PQDVKEKALKENKAPVID 100
+Y +L F + +V++ + +L P+D K+ +KENK +D
Sbjct: 502 KYMPELAGLFPHVLVDVSSVKALCIRWYPRDQKKAPIKENKHRALD 639
>AV779100
Length = 561
Score = 25.4 bits (54), Expect = 4.2
Identities = 15/41 (36%), Positives = 18/41 (43%)
Frame = -2
Query: 11 KRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPG 51
+RG S GHG R G RG +GG L +Y G
Sbjct: 257 RRGSGSGGHGGRSWRR*GGGRRGGSGGQGRELGLACRYRRG 135
>BP072692
Length = 422
Score = 25.4 bits (54), Expect = 4.2
Identities = 11/25 (44%), Positives = 12/25 (48%)
Frame = -2
Query: 12 RGHVSAGHGRIGKHRKHPGGRGNAG 36
R V G G +G H GGRG G
Sbjct: 358 RRGVGGGEGEVGSHLGR*GGRGGRG 284
>BP054828
Length = 476
Score = 25.0 bits (53), Expect = 5.4
Identities = 15/37 (40%), Positives = 20/37 (53%), Gaps = 1/37 (2%)
Frame = +2
Query: 10 KKRGHVSAGHGRIGK-HRKHPGGRGNAGGMHHHRILF 45
+K+G+ G R K R GRG+ GG+ H R LF
Sbjct: 239 RKKGNEGDGADRGQKLGRT*TDGRGHLGGVFHPRFLF 349
>TC9459 similar to AAQ23982 (AAQ23982) Transcription factor Rap212,
partial (12%)
Length = 434
Score = 25.0 bits (53), Expect = 5.4
Identities = 11/24 (45%), Positives = 14/24 (57%)
Frame = +1
Query: 21 RIGKHRKHPGGRGNAGGMHHHRIL 44
RI +HR+HP R +H HR L
Sbjct: 70 RILRHRRHPHQRHRRLHLHRHRRL 141
>AV413463
Length = 309
Score = 24.6 bits (52), Expect = 7.1
Identities = 12/28 (42%), Positives = 14/28 (49%)
Frame = +1
Query: 9 RKKRGHVSAGHGRIGKHRKHPGGRGNAG 36
R+ RGHV H +HP GRG G
Sbjct: 244 RRPRGHV---------HARHPSGRGADG 300
>TC14567 homologue to PIR|T09261|T09261 JUN kinase-activation-domain-binding
protein homolog - alfalfa {Medicago
sativa;}, partial (36%)
Length = 619
Score = 24.6 bits (52), Expect = 7.1
Identities = 10/26 (38%), Positives = 13/26 (49%)
Frame = +1
Query: 45 FDKYHPGYFGKVGMRYFHKLRNKFYN 70
F + HP + K MR FH L Y+
Sbjct: 463 FWRVHPCFISKRNMRVFHHLNRVLYS 540
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.142 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,568,451
Number of Sequences: 28460
Number of extensions: 37190
Number of successful extensions: 228
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 218
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 220
length of query: 147
length of database: 4,897,600
effective HSP length: 82
effective length of query: 65
effective length of database: 2,563,880
effective search space: 166652200
effective search space used: 166652200
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 51 (24.3 bits)
Medicago: description of AC126783.2