BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126782.12 + phase: 0
(116 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV409080 29 0.18
TC20120 weakly similar to UP|Q946Y3 (Q946Y3) SWITCH1 splice vari... 28 0.51
TC12218 27 0.67
TC13916 homologue to UP|Q9FK48 (Q9FK48) Similarity to transcript... 27 1.1
AV779960 26 1.5
AU251401 26 1.5
AV429304 26 1.9
BG662214 26 1.9
TC12795 UP|Q9FMV5 (Q9FMV5) Transcription factor Hap5a-like prote... 25 2.5
TC9220 similar to UP|Q8W1N7 (Q8W1N7) CSLC7 (Fragment), partial (... 25 2.5
TC14264 similar to UP|Q84NF9 (Q84NF9) Histone H1 (Fragment), par... 25 3.3
TC12670 similar to GB|AAP68227.1|31711742|BT008788 At5g38640 {Ar... 25 3.3
TC13280 weakly similar to GB|AAP68292.1|31711872|BT008853 At5g11... 24 5.7
AU089304 24 5.7
TC19524 weakly similar to UP|SNAA_SOLTU (Q9M5P8) Alpha-soluble N... 24 5.7
CN825096 24 7.4
TC9924 UP|RBL_LOTJA (Q9BBU1) Ribulose bisphosphate carboxylase l... 24 7.4
TC14304 similar to UP|Q94FU3 (Q94FU3) Ubiquitin conjugating enzy... 24 7.4
TC15919 similar to UP|Q9XH61 (Q9XH61) Serine carboxypeptidase, p... 24 7.4
BP085186 23 9.6
>AV409080
Length = 423
Score = 29.3 bits (64), Expect = 0.18
Identities = 28/93 (30%), Positives = 46/93 (49%), Gaps = 7/93 (7%)
Frame = -3
Query: 7 SGSSRSKRSHKDSVGSSARP----MGRE---AAKKKGIKKSKDVAGLEEVEKEWVQFKEI 59
+ +SRS +D + S P M RE A K + IK+S+ V E VE+EW + +
Sbjct: 271 ASNSRSPFILRDFIIGSPIPPLIMMTRESFKAGKSEAIKRSRHVEE-EIVEREWCGIRVV 95
Query: 60 KEQEIEHLKEYTKVQQEKNRLKKMKMYLKLSSE 92
KE I KV+++ + + + + L+L E
Sbjct: 94 KEASI-------KVEEDDEQ*RAITLKLELRIE 17
>TC20120 weakly similar to UP|Q946Y3 (Q946Y3) SWITCH1 splice variant S,
partial (9%)
Length = 557
Score = 27.7 bits (60), Expect = 0.51
Identities = 28/102 (27%), Positives = 43/102 (41%), Gaps = 2/102 (1%)
Frame = -3
Query: 8 GSSRSKRSHKDSVGSSARPMGREAAKKKGIKKSKDVAGLEEVEKEWVQFKEIKEQEI-EH 66
G R RSH P GR++ K+ KK E E+ + + EQ + E
Sbjct: 282 GRKRKSRSHGKLREVKEEPCGRQSTSKRKTKKH------ESKERWSAERYSLAEQSMWEV 121
Query: 67 LKEYTKVQQEKNRLKKMKMYLKLSSEEHLDDR-KKEHLVKHI 107
LKE + L+L++ +H+ D +HL+KHI
Sbjct: 120 LKEEGATFDN----PITRPALRLAARKHIGDTGLLDHLLKHI 7
>TC12218
Length = 737
Score = 27.3 bits (59), Expect = 0.67
Identities = 21/72 (29%), Positives = 33/72 (45%)
Frame = +2
Query: 22 SSARPMGREAAKKKGIKKSKDVAGLEEVEKEWVQFKEIKEQEIEHLKEYTKVQQEKNRLK 81
SS GR+ K S V + ++ E + KE+ E +KE ++ EKN ++
Sbjct: 335 SSILEPGRQPKTDKAAIISDAVRVVTQLRNEAEKLKEMNNDLQEKIKE---LKAEKNEIR 505
Query: 82 KMKMYLKLSSEE 93
K LKL E+
Sbjct: 506 DEKNKLKLDKEK 541
>TC13916 homologue to UP|Q9FK48 (Q9FK48) Similarity to transcription
regulator, partial (17%)
Length = 603
Score = 26.6 bits (57), Expect = 1.1
Identities = 21/74 (28%), Positives = 32/74 (42%)
Frame = +2
Query: 30 EAAKKKGIKKSKDVAGLEEVEKEWVQFKEIKEQEIEHLKEYTKVQQEKNRLKKMKMYLKL 89
EA KK IKK + + K W+Q EIK++++ E V K ++M+ +
Sbjct: 287 EADLKKEIKK---LQRYRDQIKTWIQSSEIKDKKVSASYEQALVDARKLIEREMERFKIC 457
Query: 90 SSEEHLDDRKKEHL 103
E KE L
Sbjct: 458 EKETKTKAFSKEGL 499
>AV779960
Length = 497
Score = 26.2 bits (56), Expect = 1.5
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +1
Query: 61 EQEIEHLKEYTKVQQEKNRLKKMKMYLKLSSEEHL 95
E + K+Y K +Q K RLKK + L+L HL
Sbjct: 61 EWSVPKHKKYKKREQVKKRLKK*VIKLQLGRTSHL 165
>AU251401
Length = 405
Score = 26.2 bits (56), Expect = 1.5
Identities = 17/60 (28%), Positives = 26/60 (43%)
Frame = +2
Query: 1 MGGNVGSGSSRSKRSHKDSVGSSARPMGREAAKKKGIKKSKDVAGLEEVEKEWVQFKEIK 60
MG G G + SH+D+V + + + + K KD +EVEK + IK
Sbjct: 200 MGRGRGKGKKLTVTSHEDAVSGEDEKVPMQKRRGRPQKPLKDDFDEDEVEKTEDDSENIK 379
>AV429304
Length = 355
Score = 25.8 bits (55), Expect = 1.9
Identities = 19/57 (33%), Positives = 31/57 (54%)
Frame = -2
Query: 45 GLEEVEKEWVQFKEIKEQEIEHLKEYTKVQQEKNRLKKMKMYLKLSSEEHLDDRKKE 101
GLE E E +F E+KE HL++Y + + L++ + + +EHL D K+E
Sbjct: 246 GLERREHE--EFVEVKE----HLEDYKQEKVFLEGLERKEHEEFVEVKEHLVDYKQE 94
>BG662214
Length = 363
Score = 25.8 bits (55), Expect = 1.9
Identities = 15/51 (29%), Positives = 23/51 (44%)
Frame = +1
Query: 1 MGGNVGSGSSRSKRSHKDSVGSSARPMGREAAKKKGIKKSKDVAGLEEVEK 51
MG G G + SH+D+V + + + + K KD +EVEK
Sbjct: 187 MGRGRGKGKKLTVTSHEDAVSGEDEKVPMQKRRGRPQKPLKDDFDEDEVEK 339
>TC12795 UP|Q9FMV5 (Q9FMV5) Transcription factor Hap5a-like protein, partial
(40%)
Length = 405
Score = 25.4 bits (54), Expect = 2.5
Identities = 11/38 (28%), Positives = 22/38 (56%)
Frame = +2
Query: 46 LEEVEKEWVQFKEIKEQEIEHLKEYTKVQQEKNRLKKM 83
L++ +++ F + QEIEH+ ++ Q R+KK+
Sbjct: 113 LQQQQQQLQMFWTYQRQEIEHVNDFKNHQLPLARIKKI 226
>TC9220 similar to UP|Q8W1N7 (Q8W1N7) CSLC7 (Fragment), partial (39%)
Length = 885
Score = 25.4 bits (54), Expect = 2.5
Identities = 17/58 (29%), Positives = 30/58 (51%), Gaps = 2/58 (3%)
Frame = +2
Query: 33 KKKGIKKSKDVAGLEEVEKEWVQFKEIKEQEIEHLKEYTKVQQEKNRLKKM--KMYLK 88
KK G D+ L +EK + + ++E LKE ++Q++K KK ++Y+K
Sbjct: 26 KKSGRSSEGDLVSL--IEKGPKHQRGVSVPDLEELKEEIQLQEKKASKKKKHNRIYMK 193
>TC14264 similar to UP|Q84NF9 (Q84NF9) Histone H1 (Fragment), partial (76%)
Length = 1277
Score = 25.0 bits (53), Expect = 3.3
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +2
Query: 7 SGSSRSKRSHKDSVGSSARPMGREAAKKKGIKKSKDVA 44
S ++ +K + K ++A+P + AAK K K+K VA
Sbjct: 596 SKAAATKTTAKAKPAAAAKPKAKPAAKAKPAAKAKAVA 709
>TC12670 similar to GB|AAP68227.1|31711742|BT008788 At5g38640 {Arabidopsis
thaliana;}, partial (28%)
Length = 737
Score = 25.0 bits (53), Expect = 3.3
Identities = 13/36 (36%), Positives = 21/36 (58%), Gaps = 2/36 (5%)
Frame = +2
Query: 32 AKKKGIKKSKDVA--GLEEVEKEWVQFKEIKEQEIE 65
++ + K KDV L E++K W + KEIK E++
Sbjct: 143 SRNRNPPKLKDVLFRKLNELQKLWQKLKEIKHLELQ 250
>TC13280 weakly similar to GB|AAP68292.1|31711872|BT008853 At5g11730
{Arabidopsis thaliana;}, partial (39%)
Length = 702
Score = 24.3 bits (51), Expect = 5.7
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -2
Query: 89 LSSEEHLDDRKKEHLVKH 106
+SSEEHL R + H+ H
Sbjct: 341 MSSEEHLPQRSQRHIGPH 288
>AU089304
Length = 389
Score = 24.3 bits (51), Expect = 5.7
Identities = 12/29 (41%), Positives = 16/29 (54%)
Frame = -2
Query: 2 GGNVGSGSSRSKRSHKDSVGSSARPMGRE 30
G ++G + R+ K SVGS A GRE
Sbjct: 100 GSDIGCSKIETLRAMKGSVGSEAIGEGRE 14
>TC19524 weakly similar to UP|SNAA_SOLTU (Q9M5P8) Alpha-soluble NSF
attachment protein (Alpha-SNAP)
(N-ethylmaleimide-sensitive factor attachment protein,
alpha), partial (34%)
Length = 600
Score = 24.3 bits (51), Expect = 5.7
Identities = 14/33 (42%), Positives = 17/33 (51%), Gaps = 2/33 (6%)
Frame = +2
Query: 6 GSGSSRSKRSHK--DSVGSSARPMGREAAKKKG 36
GS SSRSKR+ + DS + G KK G
Sbjct: 107 GSSSSRSKRNQRSFDSNNGRSNREGTRTCKKSG 205
>CN825096
Length = 730
Score = 23.9 bits (50), Expect = 7.4
Identities = 12/32 (37%), Positives = 18/32 (55%)
Frame = +3
Query: 74 QQEKNRLKKMKMYLKLSSEEHLDDRKKEHLVK 105
Q KN+ +M L EH+DDR K+ L++
Sbjct: 336 QAAKNKWVEMGKQLITRKVEHVDDRVKDLLLQ 431
>TC9924 UP|RBL_LOTJA (Q9BBU1) Ribulose bisphosphate carboxylase large chain
precursor (RuBisCO large subunit) , complete
Length = 1592
Score = 23.9 bits (50), Expect = 7.4
Identities = 12/40 (30%), Positives = 21/40 (52%)
Frame = -3
Query: 77 KNRLKKMKMYLKLSSEEHLDDRKKEHLVKHIWTKFGMYRD 116
+N L++ + + E+L ++ EHLVK + YRD
Sbjct: 1359 QNHLQRFRSEQAYAKREYLPKQQVEHLVKKPNPE*NKYRD 1240
>TC14304 similar to UP|Q94FU3 (Q94FU3) Ubiquitin conjugating enzyme 2
(Ubiquitin-conjugating enzyme E2) (Ubiquitin-protein
ligase) (Ubiquitin carrier protein) , partial (83%)
Length = 1428
Score = 23.9 bits (50), Expect = 7.4
Identities = 14/38 (36%), Positives = 20/38 (51%)
Frame = -3
Query: 26 PMGREAAKKKGIKKSKDVAGLEEVEKEWVQFKEIKEQE 63
P G+EAAK KG + G+E ++E E +E E
Sbjct: 130 PFGKEAAKGKGFRN----GGVESPKRERESRSESRE*E 29
>TC15919 similar to UP|Q9XH61 (Q9XH61) Serine carboxypeptidase, partial
(21%)
Length = 523
Score = 23.9 bits (50), Expect = 7.4
Identities = 15/47 (31%), Positives = 24/47 (50%)
Frame = -1
Query: 36 GIKKSKDVAGLEEVEKEWVQFKEIKEQEIEHLKEYTKVQQEKNRLKK 82
GI K D+AG EEVE +I + +E K ++E+ R ++
Sbjct: 196 GIGKDVDIAGREEVEVS----NQIVCLDRRRREEQRKEEEEEERARR 68
>BP085186
Length = 388
Score = 23.5 bits (49), Expect = 9.6
Identities = 16/52 (30%), Positives = 25/52 (47%)
Frame = -2
Query: 27 MGREAAKKKGIKKSKDVAGLEEVEKEWVQFKEIKEQEIEHLKEYTKVQQEKN 78
MG+E KKK KK K ++ +K+ + K +K + KE K K+
Sbjct: 273 MGKEMKKKKKKKKKKKKKKKKKKKKQNQRKKRMKRKMSTPKKEKEKTPSCKS 118
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.309 0.127 0.349
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,690,078
Number of Sequences: 28460
Number of extensions: 16598
Number of successful extensions: 116
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 114
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 116
length of query: 116
length of database: 4,897,600
effective HSP length: 78
effective length of query: 38
effective length of database: 2,677,720
effective search space: 101753360
effective search space used: 101753360
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 49 (23.5 bits)
Medicago: description of AC126782.12