BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126018.3 + phase: 0
(387 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC12708 NADH dehydrogenase ND2 [Lotus japonicus] 734 0.0
NP459568 NADH dehydrogenase ND5 [Lotus japonicus] 107 3e-24
NP459571 NADH dehydrogenase ND4 [Lotus japonicus] 74 5e-14
TC9694 homologue to GB|AAQ14222.1|33338911|AF238072 NADH dehydro... 57 4e-09
TC14228 homologue to UP|Q9LLR4 (Q9LLR4) Sec61p, partial (67%) 29 1.5
TC17076 28 3.4
CB828285 27 5.8
TC14740 similar to UP|Q8W584 (Q8W584) AT4g17230/dl4650c, partial... 27 5.8
BP048725 27 7.5
TC11850 27 7.5
BP056069 27 7.5
TC14472 homologue to UP|Q9AXQ2 (Q9AXQ2) Mitochondrial processing... 26 9.8
>TC12708 NADH dehydrogenase ND2 [Lotus japonicus]
Length = 1164
Score = 734 bits (1895), Expect = 0.0
Identities = 371/387 (95%), Positives = 379/387 (97%)
Frame = +1
Query: 1 MAITEFLLFILTATLGGMFLCSANDLITIFVALECFSLCSYLLSGYTKKDVRSNEATMKY 60
MAITEFLLF+LTATLGGMFLCSANDLITIFVA ECFSLCSYLLSGYTKKDVRSNEATMKY
Sbjct: 1 MAITEFLLFVLTATLGGMFLCSANDLITIFVAPECFSLCSYLLSGYTKKDVRSNEATMKY 180
Query: 61 LLMGGASSSILVHGFSWLYGLSGGEIELQEIVNGLINTQMYNSPGISIALIFITVGIGFK 120
LLMGGASSSILVHGFSWLYG SGGEIELQEIVNGLINTQMYNSPGISIALIFITVGIGFK
Sbjct: 181 LLMGGASSSILVHGFSWLYGSSGGEIELQEIVNGLINTQMYNSPGISIALIFITVGIGFK 360
Query: 121 LSLAPSHQWTPDVYEGSPTPVVAFLSVTSKVAASASATRIFDILFYFSSNEWHLLLEILA 180
LSLAPSHQWTPDVYEGSPTPVVAFLSVTSKVAASA ATRIFDI FYFSSNEWHLLLEILA
Sbjct: 361 LSLAPSHQWTPDVYEGSPTPVVAFLSVTSKVAASALATRIFDIPFYFSSNEWHLLLEILA 540
Query: 181 ILSMLLGNLIAITQTSMKRMLAYSSIGQIGYVIIGIIVGDSNDGYASMITYMLFYISMNL 240
ILSM+LGNLIAITQTSMKRMLAYSSIGQIGYVIIGIIVGDSN GYASMITYMLFYISMNL
Sbjct: 541 ILSMILGNLIAITQTSMKRMLAYSSIGQIGYVIIGIIVGDSNGGYASMITYMLFYISMNL 720
Query: 241 GTFACIVLFGLRTGTDNIRDYAGLYTKDPFLALSLALCLLSLGGLPPLAGFFGKLHLFWC 300
GTFACIV FGLRTGTDNIRDYAGLYTKDPFLALSLALCLLSLGGLPPLAGFFGKL+LFWC
Sbjct: 721 GTFACIVSFGLRTGTDNIRDYAGLYTKDPFLALSLALCLLSLGGLPPLAGFFGKLYLFWC 900
Query: 301 GWQAGLYFLVSIGLITSVVSIYYYLKIIKLLMTGRNQEITPHVRNYRRSPVRSNNSIELS 360
GWQAGLYFLVSIGL+ SVVSIYYYLKIIKLLMTGRNQEITPHVRNY+RSP+RSNNSIELS
Sbjct: 901 GWQAGLYFLVSIGLLMSVVSIYYYLKIIKLLMTGRNQEITPHVRNYKRSPLRSNNSIELS 1080
Query: 361 MIICVIASTVPGISMNPIIEIAQDTLF 387
MI+CVIAST+ GISMNPIIEIAQDTLF
Sbjct: 1081MIVCVIASTISGISMNPIIEIAQDTLF 1161
>NP459568 NADH dehydrogenase ND5 [Lotus japonicus]
Length = 2244
Score = 107 bits (267), Expect = 3e-24
Identities = 94/361 (26%), Positives = 163/361 (45%), Gaps = 7/361 (1%)
Frame = +1
Query: 6 FLLFILTATLGGMFLCSANDLITIFVALECFSLCSYLLSGYTKKDVRSNEATMKYLLMGG 65
+L F T+ LG L ++++LI +++ E +CSYLL G+ + A K +
Sbjct: 379 YLSFFNTSMLG---LVTSSNLIQVYIFWELVGMCSYLLIGFWFTRPIAANACQKAFVTNR 549
Query: 66 ASSSILVHGFSWLYGLSGGEI--ELQEIVNGLINTQMYNSPGISIALIFITVGIGFKLSL 123
L+ G LY ++G +L +I+N LI+ N I++ + + G K +
Sbjct: 550 VGDFGLLLGILGLYWITGSLEFRDLFQIINNLIDQNEVNIFFITLCALLLFCGSVAKSAQ 729
Query: 124 APSHQWTPDVYEGSPTPVVAFLSVTSKVAASASATRIFDILFYFSSNEWHLLLEILAILS 183
P H W PD EG PTP+ A + + VAA + F+ + + + I++
Sbjct: 730 FPLHVWLPDAMEG-PTPISALIHAATMVAAGIFLVARL-LPFFIAIPSIMNGIAFIGIIT 903
Query: 184 MLLGNLIAITQTSMKRMLAYSSIGQIGYVIIGIIVGDSNDGYASMITYMLFYISMNLGTF 243
++LG +AI Q +K+ LAYS++ Q+GY+++ + +G +IT+ + LG+
Sbjct: 904 VVLGATLAIAQKDIKKNLAYSTMSQLGYMMLALGMGSYRIALFHLITHAYSKALLFLGSG 1083
Query: 244 ACIVLFGLRTG-----TDNIRDYAGLYTKDPFLALSLALCLLSLGGLPPLAGFFGKLHLF 298
+ I G + N+ GL P + + LSL G+PP A F+ K +
Sbjct: 1084SIIHSMEAIVGYSPNKSQNMLLMGGLTKHVPITKTAFLIGTLSLCGIPPFACFWSKDEIL 1263
Query: 299 WCGWQAGLYFLVSIGLITSVVSIYYYLKIIKLLMTGRNQEITPHVRNYRRSPVRSNNSIE 358
W F + I T+ ++ +Y +I L+ G H +N+ S SI
Sbjct: 1264NDSWLYSPIFAI-IACSTAGLTAFYMFRIYLLVFEG---YFNVHFQNFNGKKNSSFYSIS 1431
Query: 359 L 359
L
Sbjct: 1432L 1434
>NP459571 NADH dehydrogenase ND4 [Lotus japonicus]
Length = 1494
Score = 73.6 bits (179), Expect = 5e-14
Identities = 87/373 (23%), Positives = 151/373 (40%), Gaps = 12/373 (3%)
Frame = +1
Query: 6 FLLFILTATLGGMFLCSANDLITIFVALECFSLCSYLLSGYTKKDVRSNEATMKYLLMGG 65
F +L G + S+ D++ F+ E + YLL R AT L G
Sbjct: 340 FYFLMLAMYSGQIGPFSSRDILLFFIMWELELIPVYLLLAMWGGKKRLYSATKFILYTAG 519
Query: 66 ASSSILVHGFS-WLYGLSGGEIELQEIVNGLINTQMYNSPGISIALIFITVGIGFKLSLA 124
+S +L+ LYG + + + + N Q Y I I + K +
Sbjct: 520 SSVFLLMATLGIGLYGSNEPTLNFETL-----NNQSYPLALEIIVYIGFLIAFAVKSPII 684
Query: 125 PSHQWTPDVYEGSPTPVVAFLSVTSKVAASASATRIFDILFYFSSNEWHLLLEILAILSM 184
P H W PD + + L+ + RI LF + + + L IL + +
Sbjct: 685 PLHTWLPDTHGEAHYSTCMLLAGILLKMGAYGLVRINMELFSHAHSIFCPWLMILGSIQI 864
Query: 185 LLGNLIAITQTSMKRMLAYSSIGQIGYVIIGIIVGDSNDGYASMITYMLFYISMNLGTFA 244
+ ++ Q ++K+ +AYSS+ +G++IIG I S+ G I ++ + G
Sbjct: 865 IYAASASLGQRNLKKRIAYSSVSHMGFLIIG-IGSISDTGLNGAILQIISH-----GFIG 1026
Query: 245 CIVLFGLRTGTDNIR-----DYAGLYTKDPFLALSLALCLLSLGGLPPLAGFFGKLHLFW 299
+ F T D +R + G+ P + + ++ LP ++GFF +L +FW
Sbjct: 1027AALFFLSGTSYDRLRLLYLDEMGGMALPMPKIFTVFTILSMASLALPGMSGFFAELIVFW 1206
Query: 300 CGWQAGLYFLVSIGLITSVVSI------YYYLKIIKLLMTGRNQEITPHVRNYRRSPVRS 353
+ YFL+ LIT V +I Y L I++ + G TP+ + P
Sbjct: 1207GIITSQKYFLIMKILITFVTAIGMILTPIYSLSILRQMFYGYKFFNTPNSYFFDSGP--R 1380
Query: 354 NNSIELSMIICVI 366
I +S++I +I
Sbjct: 1381ELFISISILIPII 1419
>TC9694 homologue to GB|AAQ14222.1|33338911|AF238072 NADH dehydrogenase
subunit B {Pisum sativum;} , partial (12%)
Length = 590
Score = 57.4 bits (137), Expect = 4e-09
Identities = 29/43 (67%), Positives = 34/43 (78%)
Frame = +1
Query: 187 GNLIAITQTSMKRMLAYSSIGQIGYVIIGIIVGDSNDGYASMI 229
G + + + ++KRMLAYSSIGQIGYVIIGIIVG SN G SMI
Sbjct: 289 GISLLLPKQALKRMLAYSSIGQIGYVIIGIIVGGSNGGCGSMI 417
>TC14228 homologue to UP|Q9LLR4 (Q9LLR4) Sec61p, partial (67%)
Length = 1268
Score = 28.9 bits (63), Expect = 1.5
Identities = 12/33 (36%), Positives = 22/33 (66%)
Frame = +1
Query: 6 FLLFILTATLGGMFLCSANDLITIFVALECFSL 38
+LL+ L++ +F C N+L T FV+ +C++L
Sbjct: 1027 WLLWFLSSA*ELLFSCDTNELSTTFVSRDCYTL 1125
>TC17076
Length = 644
Score = 27.7 bits (60), Expect = 3.4
Identities = 14/51 (27%), Positives = 27/51 (52%), Gaps = 3/51 (5%)
Frame = +3
Query: 89 QEIVNGLINTQMYNSPG---ISIALIFITVGIGFKLSLAPSHQWTPDVYEG 136
+E + IN Y PG + I L+ ++ G+ ++ L ++WT + +EG
Sbjct: 27 RETLRADINIGRYQPPGEEKMDINLVIVSHGLTLRVFLMRWYKWTVEQFEG 179
>CB828285
Length = 552
Score = 26.9 bits (58), Expect = 5.8
Identities = 21/65 (32%), Positives = 29/65 (44%)
Frame = +2
Query: 119 FKLSLAPSHQWTPDVYEGSPTPVVAFLSVTSKVAASASATRIFDILFYFSSNEWHLLLEI 178
F L +P H+W ++ P P L +S V F I FS+N LLL I
Sbjct: 119 FFLRPSPPHRWW---FQRRPPPQAVLLYASSFVYFRRCKACYFFIPHSFSTNRSSLLLFI 289
Query: 179 LAILS 183
L+ L+
Sbjct: 290 LSSLN 304
>TC14740 similar to UP|Q8W584 (Q8W584) AT4g17230/dl4650c, partial (57%)
Length = 1406
Score = 26.9 bits (58), Expect = 5.8
Identities = 17/50 (34%), Positives = 29/50 (58%), Gaps = 2/50 (4%)
Frame = +1
Query: 133 VYEGSPTPVVAFLSVTSKVA-ASASATRIFDILFYFSSN-EWHLLLEILA 180
+Y+ P A++S + +A A A+ +RI I F + +WHLL++ LA
Sbjct: 160 LYQICPYWKFAYISANAAIAEAMANESRIHIIDFQIAQGTQWHLLIQALA 309
>BP048725
Length = 500
Score = 26.6 bits (57), Expect = 7.5
Identities = 25/64 (39%), Positives = 32/64 (49%), Gaps = 2/64 (3%)
Frame = +1
Query: 120 KLSLAPSHQWTPDVYEGSPTPVVAFLS-VTSKVAASASAT-RIFDILFYFSSNEWHLLLE 177
+L L+P P S T +A L V ++VAA A +FD+L F S E HL L
Sbjct: 262 RLLLSPKGSPVPSXLNLSSTKAMAGLDMVAARVAAIKDAPIPMFDLLS-FPSMEQHLTLP 438
Query: 178 ILAI 181
LAI
Sbjct: 439 TLAI 450
>TC11850
Length = 495
Score = 26.6 bits (57), Expect = 7.5
Identities = 12/40 (30%), Positives = 23/40 (57%)
Frame = +3
Query: 1 MAITEFLLFILTATLGGMFLCSANDLITIFVALECFSLCS 40
M ++ F ++ A++ C NDL+ +FV+ ++LCS
Sbjct: 324 MIVSRFSFYLFGASIA----CLCNDLVCMFVSKINYNLCS 431
>BP056069
Length = 373
Score = 26.6 bits (57), Expect = 7.5
Identities = 16/48 (33%), Positives = 25/48 (51%), Gaps = 6/48 (12%)
Frame = -3
Query: 342 HVRNYRRSP------VRSNNSIELSMIICVIASTVPGISMNPIIEIAQ 383
H R Y+ P + SNN + +S+IIC+ + V + PI+E Q
Sbjct: 155 HPRGYQPKPPLSSIQLLSNNPLFMSLIICISLAFVLLLYKFPILEPCQ 12
>TC14472 homologue to UP|Q9AXQ2 (Q9AXQ2) Mitochondrial processing peptidase
beta subunit, partial (18%)
Length = 665
Score = 26.2 bits (56), Expect = 9.8
Identities = 12/35 (34%), Positives = 23/35 (65%)
Frame = +2
Query: 352 RSNNSIELSMIICVIASTVPGISMNPIIEIAQDTL 386
R +++E+ M +C+ STV G+S+ P+ + +TL
Sbjct: 149 RKKHAVEVPMAVCLHGSTVTGMSL-PLFILHIETL 250
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.326 0.142 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,670,978
Number of Sequences: 28460
Number of extensions: 95218
Number of successful extensions: 737
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 726
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 732
length of query: 387
length of database: 4,897,600
effective HSP length: 92
effective length of query: 295
effective length of database: 2,279,280
effective search space: 672387600
effective search space used: 672387600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC126018.3