BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126014.4 + phase: 0
(91 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV418280 43 8e-06
BP057427 39 2e-04
AV427022 37 6e-04
TC14968 similar to UP|Q8TTE6 (Q8TTE6) Bacterial extracellular so... 36 0.001
BP079663 36 0.001
BP034765 33 0.009
BP039473 33 0.011
BP057455 32 0.015
TC11392 similar to UP|Q8BGI0 (Q8BGI0) Sterol-C5-desaturase, part... 32 0.015
AV411298 32 0.015
TC15601 31 0.033
TC13043 similar to GB|BAB21544.1|12641847|AB022782 hydroxyprolin... 31 0.033
BP033684 31 0.033
BP033775 30 0.073
TC16776 30 0.095
AU089120 29 0.16
AV774307 28 0.28
BP065246 28 0.36
BP040769 27 0.62
TC11605 27 0.62
>AV418280
Length = 398
Score = 43.1 bits (100), Expect = 8e-06
Identities = 22/64 (34%), Positives = 33/64 (51%)
Frame = -2
Query: 26 IWHACVWLIWKETNSRIFNHKTKEVGLLLDSVKLTSFLWLKANKLTSAFSYTDRWRDPLL 85
IW A VW +W + NS IF + + LLD ++ S+ WLKA +S + PL
Sbjct: 196 IWIATVWSLWIQRNSIIFRNNALDHSYLLDLIQSRSWHWLKAKFCGFTYSLYEWKSCPLE 17
Query: 86 CMGA 89
C+ +
Sbjct: 16 CLSS 5
>BP057427
Length = 480
Score = 38.9 bits (89), Expect = 2e-04
Identities = 20/62 (32%), Positives = 31/62 (49%)
Frame = -1
Query: 26 IWHACVWLIWKETNSRIFNHKTKEVGLLLDSVKLTSFLWLKANKLTSAFSYTDRWRDPLL 85
IW A + IW NS IF + + L+D ++ S+ WLKA ++S + PL
Sbjct: 399 IWVAALASIWYSRNSLIFKGEELDFQRLVDQIQTKSWSWLKAKVTGFSYSLIEWKSQPLS 220
Query: 86 CM 87
C+
Sbjct: 219 CL 214
>AV427022
Length = 429
Score = 37.0 bits (84), Expect = 6e-04
Identities = 16/62 (25%), Positives = 34/62 (54%)
Frame = +1
Query: 26 IWHACVWLIWKETNSRIFNHKTKEVGLLLDSVKLTSFLWLKANKLTSAFSYTDRWRDPLL 85
IW + +W IW N +FN + LL+ +++T++ WL++ +S+ + +P +
Sbjct: 232 IWMSVLWSIWCLRNMVVFNGGELDKDQLLEQIQVTAWRWLRSRLDDF*YSWYEWKSNPRI 411
Query: 86 CM 87
C+
Sbjct: 412 CL 417
>TC14968 similar to UP|Q8TTE6 (Q8TTE6) Bacterial extracellular
solute-binding family 3 protein, partial (3%)
Length = 503
Score = 36.2 bits (82), Expect = 0.001
Identities = 17/62 (27%), Positives = 28/62 (44%)
Frame = +1
Query: 26 IWHACVWLIWKETNSRIFNHKTKEVGLLLDSVKLTSFLWLKANKLTSAFSYTDRWRDPLL 85
+W A VW +W N +F + VKL + WLKA + + + +P+L
Sbjct: 211 VWLAVVWHVWVGRNEAVFREGVFLPECIFYLVKLKVWEWLKARSCHFTHATYEWFNEPIL 390
Query: 86 CM 87
C+
Sbjct: 391 CL 396
>BP079663
Length = 434
Score = 35.8 bits (81), Expect = 0.001
Identities = 13/45 (28%), Positives = 25/45 (54%)
Frame = -2
Query: 25 VIWHACVWLIWKETNSRIFNHKTKEVGLLLDSVKLTSFLWLKANK 69
V+W + +W IW N+ IF + + ++D ++ S+ WL+ K
Sbjct: 400 VVWVSVIWSIWTMRNACIFKGEVPALDRVMDLIQFRSWSWLREKK 266
>BP034765
Length = 506
Score = 33.1 bits (74), Expect = 0.009
Identities = 16/59 (27%), Positives = 25/59 (42%)
Frame = -2
Query: 29 ACVWLIWKETNSRIFNHKTKEVGLLLDSVKLTSFLWLKANKLTSAFSYTDRWRDPLLCM 87
A W +W+ N +FN + + D +L WL A FS + P+LC+
Sbjct: 493 AMAWALWEHRNWIVFNGGLLQEDSIFDKAQLKVCQWLSAKGKDFHFSMYEWLSQPILCL 317
>BP039473
Length = 501
Score = 32.7 bits (73), Expect = 0.011
Identities = 13/56 (23%), Positives = 27/56 (48%)
Frame = -3
Query: 32 WLIWKETNSRIFNHKTKEVGLLLDSVKLTSFLWLKANKLTSAFSYTDRWRDPLLCM 87
W +W N +F + KE G +++ L ++ WL+ + +S + P +C+
Sbjct: 466 WSLWLHWNDILFKNDQKEEGRVVEIAPLRAWNWLRVRNHSFTYSLYEWLTPPRICL 299
>BP057455
Length = 523
Score = 32.3 bits (72), Expect = 0.015
Identities = 14/34 (41%), Positives = 23/34 (67%)
Frame = -1
Query: 57 VKLTSFLWLKANKLTSAFSYTDRWRDPLLCMGAR 90
+K S+LW+KAN ++ +S + +PLLC+ AR
Sbjct: 451 IKWRSWLWIKANIISFPYSVFEWSSNPLLCIXAR 350
>TC11392 similar to UP|Q8BGI0 (Q8BGI0) Sterol-C5-desaturase, partial (5%)
Length = 579
Score = 32.3 bits (72), Expect = 0.015
Identities = 19/60 (31%), Positives = 32/60 (52%), Gaps = 1/60 (1%)
Frame = +3
Query: 29 ACVWLIWKETNSRIFNHKTKEVGLLLDSVKLTSFLWLKANKLTSAFSYTDRWR-DPLLCM 87
A VW + NS +F+ + +LDS+ S+ WLK +K+ S W+ +PL+C+
Sbjct: 18 ATVWSLXLVRNSILFSGGVLDKANILDSIPWRSWFWLK-SKMDGFTSSLFEWKTNPLICI 194
>AV411298
Length = 429
Score = 32.3 bits (72), Expect = 0.015
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = +1
Query: 22 FLQVIWHACVWLIWKETNSRIFN 44
F +VIW A W IW+ N IFN
Sbjct: 361 FFRVIWFATAWTIWRHRNCVIFN 429
>TC15601
Length = 613
Score = 31.2 bits (69), Expect = 0.033
Identities = 16/61 (26%), Positives = 29/61 (47%)
Frame = +2
Query: 29 ACVWLIWKETNSRIFNHKTKEVGLLLDSVKLTSFLWLKANKLTSAFSYTDRWRDPLLCMG 88
A W +W N IF + +++ +L ++ WL+A K FS + +P C+G
Sbjct: 92 AIAWTLWNLRNMIIFKEGHLDGERVVEIAQLRAWNWLRAKKKDFKFSTYEWVVNPRYCIG 271
Query: 89 A 89
+
Sbjct: 272 S 274
>TC13043 similar to GB|BAB21544.1|12641847|AB022782 hydroxyproline-rich
glycoprotein {Arabidopsis thaliana;} , partial (6%)
Length = 678
Score = 31.2 bits (69), Expect = 0.033
Identities = 11/39 (28%), Positives = 19/39 (48%)
Frame = +3
Query: 12 LAGAPRSTHTFLQVIWHACVWLIWKETNSRIFNHKTKEV 50
L G R + +V+W A +W +W N+ +F E+
Sbjct: 549 LMGLNRQQNKLWKVVWLAVIWTVWNGRNNLVFREVEMEI 665
>BP033684
Length = 557
Score = 31.2 bits (69), Expect = 0.033
Identities = 18/63 (28%), Positives = 28/63 (43%)
Frame = -1
Query: 25 VIWHACVWLIWKETNSRIFNHKTKEVGLLLDSVKLTSFLWLKANKLTSAFSYTDRWRDPL 84
+I +A VW +W E +F K + D +T F W+KA +++ RD
Sbjct: 512 LILYALVWSLWLERKQSVFKGKLASSQEVWDLHLMTIFWWIKAVWKECPYNFDQFHRD-- 339
Query: 85 LCM 87
CM
Sbjct: 338 FCM 330
>BP033775
Length = 530
Score = 30.0 bits (66), Expect = 0.073
Identities = 17/52 (32%), Positives = 28/52 (53%), Gaps = 2/52 (3%)
Frame = -2
Query: 39 NSRIFNHKTKEVGLLLDSVKLTSFLWLKANKLTSAFSYTD-RWR-DPLLCMG 88
N +FN KT ++D +K ++ WL+ T FSY+ W +P +C+G
Sbjct: 391 NDVLFNGKTINSEAVVDLIKFKAWKWLQCK--TKDFSYSSYAWNWNPCICLG 242
>TC16776
Length = 606
Score = 29.6 bits (65), Expect = 0.095
Identities = 17/54 (31%), Positives = 27/54 (49%)
Frame = +1
Query: 12 LAGAPRSTHTFLQVIWHACVWLIWKETNSRIFNHKTKEVGLLLDSVKLTSFLWL 65
L G+ + T L +IW A V LIW N F + E+ +D+V+ ++ L
Sbjct: 88 LPGSKKVAQT-LTLIWVATVGLIWNSRNGAEFREEVVEICKSVDTVQFKGWVLL 246
>AU089120
Length = 586
Score = 28.9 bits (63), Expect = 0.16
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = +3
Query: 54 LDSVKLTSFLWLKANKLTSAFSYTDRWRDPLLCM 87
LDS++ S+LWLK FS + +PL+C+
Sbjct: 315 LDSIQYRSWLWLKGKVKGLCFSLYEWSSNPLVCL 416
Score = 28.5 bits (62), Expect = 0.21
Identities = 13/50 (26%), Positives = 24/50 (48%), Gaps = 2/50 (4%)
Frame = +2
Query: 23 LQVIWHACVWLIWKETNSRIFNHKTKEV--GLLLDSVKLTSFLWLKANKL 70
L IW + +W IW N +FN + + G DS+ + + K+ ++
Sbjct: 221 LGFIWLSTIWFIWHLRNKVVFNGRCFRLVRGFRFDSISIVAMAKRKSERV 370
>AV774307
Length = 457
Score = 28.1 bits (61), Expect = 0.28
Identities = 14/39 (35%), Positives = 26/39 (65%)
Frame = -1
Query: 38 TNSRIFNHKTKEVGLLLDSVKLTSFLWLKANKLTSAFSY 76
T R+F+ K V L+ ++V++TSF LK+++L++ Y
Sbjct: 205 TQIRLFSCKLDSVYLIPENVRITSFETLKSSQLSAYGHY 89
>BP065246
Length = 505
Score = 27.7 bits (60), Expect = 0.36
Identities = 15/51 (29%), Positives = 26/51 (50%)
Frame = -2
Query: 39 NSRIFNHKTKEVGLLLDSVKLTSFLWLKANKLTSAFSYTDRWRDPLLCMGA 89
N IF ++ ++ D ++L ++ WLKA FS D +P C+G+
Sbjct: 444 NKVIFRNEEVDLERAWDLIQLRAWNWLKAGCSKFHFSIYDWISNPRYCIGS 292
>BP040769
Length = 549
Score = 26.9 bits (58), Expect = 0.62
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = -3
Query: 23 LQVIWHACVWLIWKETNSRIF 43
L IW A VW +W NS++F
Sbjct: 112 LMSIWVAVVWGVWLGRNSKVF 50
>TC11605
Length = 546
Score = 26.9 bits (58), Expect = 0.62
Identities = 18/59 (30%), Positives = 24/59 (40%), Gaps = 4/59 (6%)
Frame = +1
Query: 24 QVIWH----ACVWLIWKETNSRIFNHKTKEVGLLLDSVKLTSFLWLKANKLTSAFSYTD 78
+V+W A VW IW N +FN K + D + W KA +S TD
Sbjct: 109 RVLWELVPCAVVWSIWLARNDLVFNQKEFIYENVWDLHLMRVMWWTKAWWKGCPYSTTD 285
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.326 0.137 0.464
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,237,316
Number of Sequences: 28460
Number of extensions: 32408
Number of successful extensions: 268
Number of sequences better than 10.0: 62
Number of HSP's better than 10.0 without gapping: 268
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 268
length of query: 91
length of database: 4,897,600
effective HSP length: 67
effective length of query: 24
effective length of database: 2,990,780
effective search space: 71778720
effective search space used: 71778720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 48 (23.1 bits)
Medicago: description of AC126014.4