BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126012.8 + phase: 0
(440 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF177509 68 3e-12
BP030148 65 2e-11
AV766619 43 1e-04
BP047855 32 0.27
TC8795 similar to UP|GMD1_ARATH (Q9SNY3) GDP-mannose 4,6 dehydra... 30 1.0
AV767794 29 1.3
TC10371 29 1.8
TC8003 homologue to PIR|S52023|S52023 translation initiation fac... 28 2.3
TC15475 similar to UP|Q93VZ2 (Q93VZ2) AT4g01050/F2N1_31, partial... 28 2.3
TC9473 similar to AAS09986 (AAS09986) MYB transcription factor, ... 28 2.3
AV406521 28 3.0
BP039068 28 3.9
BP039650 27 6.7
TC10608 weakly similar to UP|CAD41394 (CAD41394) OJ000223_09.7 p... 27 6.7
TC17917 similar to GB|AAO63324.1|28950801|BT005260 At5g06770 {Ar... 27 6.7
BP036551 27 8.7
>BF177509
Length = 472
Score = 68.2 bits (165), Expect = 3e-12
Identities = 41/156 (26%), Positives = 76/156 (48%)
Frame = +1
Query: 190 WVDNIPKEKWTQAYDEGRRWGHMTSNIVESWNSVFKGTRNLPVTPIVQSTYYRLACLFAD 249
W+ IP W AY EG+R+GH+T+NIVES N+ LP+ +++ +L F +
Sbjct: 19 WIRRIPPRLWATAYFEGQRFGHLTANIVESLNTWILEASGLPIIQMMECIRRQLMTWFNE 198
Query: 250 RAQKAFARVGSGDLFSEYCQNAIQDDIVKSNTHHVEQFDRERYTFSVRETVNYREGRPMG 309
R + + + + + + + + ++ T+ V + + + E +++ G
Sbjct: 199 RRETS---MQWASILVPSAERCVAEALERARTYQVLRANEAEF-----EVISHE-----G 339
Query: 310 TFKVDLRAGWCDCGKFQALHLPCSHVIAACSSFRHD 345
+ VD+R C C +Q LPC+H +AA S R +
Sbjct: 340 SNIVDIRNRCCLCRGWQLYGLPCAHAVAALLSCRQN 447
>BP030148
Length = 398
Score = 65.1 bits (157), Expect = 2e-11
Identities = 32/101 (31%), Positives = 54/101 (52%)
Frame = +3
Query: 137 HVYCIRHIKQNFMRTIKDGDLKDVVNNMGYALNVPLFNYYRGVIQEANQRALDWVDNIPK 196
H +C+RH+ +F + + L +++ + L V F I+E +Q A W+ IP
Sbjct: 90 HGFCMRHLSDSFRKEFNNTMLVNLLWEAAHVLTVIEFESKILEIEEISQDAAYWIRRIPP 269
Query: 197 EKWTQAYDEGRRWGHMTSNIVESWNSVFKGTRNLPVTPIVQ 237
W AY EG+R+GH+T+NIVES ++ LP+ + +
Sbjct: 270 RLWATAYFEGQRFGHLTANIVESLDTWILDASGLPIVQMTE 392
>AV766619
Length = 412
Score = 42.7 bits (99), Expect = 1e-04
Identities = 23/107 (21%), Positives = 47/107 (43%), Gaps = 7/107 (6%)
Frame = +2
Query: 137 HVYCIRHIKQNFMRTIKDGDLKD----VVNNMGYALNVPLFNYYRGVIQEA---NQRALD 189
H YC+R++ + + +K + ++N+ A P + ++ + A +
Sbjct: 89 HSYCLRYLAEKLNKDLKGQFSHEARRFMINDFYAAAYAPKQETFERSVENMKGISSEAFN 268
Query: 190 WVDNIPKEKWTQAYDEGRRWGHMTSNIVESWNSVFKGTRNLPVTPIV 236
WV E W A+ G R+ H+TSN + + + LP+T ++
Sbjct: 269 WVMQNEPEHWANAFFNGARYNHLTSNFGQQFYNWMSEAHELPITQMI 409
>BP047855
Length = 503
Score = 31.6 bits (70), Expect = 0.27
Identities = 27/93 (29%), Positives = 41/93 (44%), Gaps = 4/93 (4%)
Frame = -1
Query: 103 VTKGISVCMVSDRH-ESIKSAFNDPRNGWQATGSAHVYCIRHIKQNFMRTIKDGDLKDVV 161
+ +G S C D+ E++K A R GW+A CI H+K+ + L+ +
Sbjct: 497 LARGYSSCKDMDKALETMKKAILAGRPGWKAYPFTFAACIEHVKEKGDSELASEILR-IC 321
Query: 162 NNMGY---ALNVPLFNYYRGVIQEANQRALDWV 191
Y A + L +Y G I E N ALD +
Sbjct: 320 GERDYFTAATHDKLLSYMHGEIPETN--ALDLI 228
>TC8795 similar to UP|GMD1_ARATH (Q9SNY3) GDP-mannose 4,6 dehydratase
(GDP-D-mannose dehydratase) (GMD) , partial (43%)
Length = 674
Score = 29.6 bits (65), Expect = 1.0
Identities = 17/42 (40%), Positives = 20/42 (47%)
Frame = -3
Query: 391 RRLKKGRPNSTRIRTEMDEEVVERTPTPRRCGLCRHTGHIRR 432
RR ++ R S R E V R PRR G CR +G RR
Sbjct: 525 RRWRRRRRCSQGSRRTQQHETVRRDCRPRRGGWCRASGGARR 400
>AV767794
Length = 432
Score = 29.3 bits (64), Expect = 1.3
Identities = 20/71 (28%), Positives = 30/71 (42%), Gaps = 3/71 (4%)
Frame = -3
Query: 117 ESIKSAFNDPRNGWQATGSAHVYCIRHIKQNFMRTIKDGDLKDVVNNMGY---ALNVPLF 173
E++K A R GW+ CI H+K+ + L+ + GY A + L
Sbjct: 397 ETLKKAMLAGRPGWKVQPFTFASCIEHVKEKRDSELASEILR-ICGERGYFTAATHDRLL 221
Query: 174 NYYRGVIQEAN 184
+Y G I E N
Sbjct: 220 SYMHGEIPEVN 188
>TC10371
Length = 823
Score = 28.9 bits (63), Expect = 1.8
Identities = 14/38 (36%), Positives = 18/38 (46%)
Frame = +2
Query: 303 REGRPMGTFKVDLRAGWCDCGKFQALHLPCSHVIAACS 340
RE P+GTF V GW C F C+ + +CS
Sbjct: 326 REIYPIGTFTVSQGEGWRSCSMFTGKGSLCTLL*CSCS 439
>TC8003 homologue to PIR|S52023|S52023 translation initiation factor
eIF-4A.14 - common tobacco {Nicotiana tabacum;} ,
partial (34%)
Length = 708
Score = 28.5 bits (62), Expect = 2.3
Identities = 9/14 (64%), Positives = 12/14 (85%)
Frame = +2
Query: 32 FWAFYPCINGFKYC 45
FWAF+ CI+GF+ C
Sbjct: 491 FWAFFCCISGFELC 532
>TC15475 similar to UP|Q93VZ2 (Q93VZ2) AT4g01050/F2N1_31, partial (60%)
Length = 1271
Score = 28.5 bits (62), Expect = 2.3
Identities = 18/57 (31%), Positives = 28/57 (48%), Gaps = 3/57 (5%)
Frame = -3
Query: 386 HNPNMRRLKKGRPNSTRIRTEMDEEVVERTPTPR---RCGLCRHTGHIRRNCPSLNN 439
H PN+ R + ST I T++ ++ R TPR L ++ H + + PS NN
Sbjct: 630 HLPNLHRCSYVKQLSTPISTKLHIRILRRLNTPRLRLLQNLRQNQWHCQNSNPSSNN 460
>TC9473 similar to AAS09986 (AAS09986) MYB transcription factor, partial
(50%)
Length = 887
Score = 28.5 bits (62), Expect = 2.3
Identities = 10/18 (55%), Positives = 11/18 (60%)
Frame = +3
Query: 419 RRCGLCRHTGHIRRNCPS 436
RRC C H GH R CP+
Sbjct: 189 RRCSHCSHGGHNARTCPN 242
>AV406521
Length = 267
Score = 28.1 bits (61), Expect = 3.0
Identities = 15/67 (22%), Positives = 30/67 (44%)
Frame = +3
Query: 101 RHVTKGISVCMVSDRHESIKSAFNDPRNGWQATGSAHVYCIRHIKQNFMRTIKDGDLKDV 160
+H T GI V + + F++ S+H +C+RH+ ++ + K+ L +
Sbjct: 90 KHATDGIFVRWAKGYYRRSEDEFSN---------SSHTFCMRHLSESIGKEFKNSRLVHL 242
Query: 161 VNNMGYA 167
+ YA
Sbjct: 243 LWKAAYA 263
>BP039068
Length = 467
Score = 27.7 bits (60), Expect = 3.9
Identities = 17/53 (32%), Positives = 20/53 (37%)
Frame = +3
Query: 388 PNMRRLKKGRPNSTRIRTEMDEEVVERTPTPRRCGLCRHTGHIRRNCPSLNNR 440
P M +G ST V P C C+ TGH +CPSL R
Sbjct: 231 PRMPARARGPTAST------SSNVNHNNPRQTVCMNCQETGHASNDCPSLTLR 371
>BP039650
Length = 488
Score = 26.9 bits (58), Expect = 6.7
Identities = 11/30 (36%), Positives = 20/30 (66%)
Frame = -2
Query: 378 PYDGPVICHNPNMRRLKKGRPNSTRIRTEM 407
P+ P++CH PN+ + K+ RP + +I E+
Sbjct: 322 PFPKPLLCHAPNI*K-KQHRPTTIKICNEL 236
>TC10608 weakly similar to UP|CAD41394 (CAD41394) OJ000223_09.7 protein,
partial (35%)
Length = 820
Score = 26.9 bits (58), Expect = 6.7
Identities = 14/42 (33%), Positives = 20/42 (47%)
Frame = +3
Query: 361 SIYNTAFKVVHDKSYWLPYDGPVICHNPNMRRLKKGRPNSTR 402
S+YN ++S W P P++C N N+ R PN R
Sbjct: 411 SLYNL------NQSLWSPPSHPLLCRNQNLLRKSLLNPNG*R 518
>TC17917 similar to GB|AAO63324.1|28950801|BT005260 At5g06770 {Arabidopsis
thaliana;}, partial (35%)
Length = 587
Score = 26.9 bits (58), Expect = 6.7
Identities = 15/67 (22%), Positives = 29/67 (42%)
Frame = -3
Query: 164 MGYALNVPLFNYYRGVIQEANQRALDWVDNIPKEKWTQAYDEGRRWGHMTSNIVESWNSV 223
MG ++ F +R +I A+ +W E W + + W T++ ++
Sbjct: 468 MGKVASITKFATFR*IILVAHAGFNNWRRRSIWEGWWRNWISPHLWNSRTASNIDHLRDS 289
Query: 224 FKGTRNL 230
F+ TRN+
Sbjct: 288 FEATRNI 268
>BP036551
Length = 428
Score = 26.6 bits (57), Expect = 8.7
Identities = 12/44 (27%), Positives = 18/44 (40%)
Frame = -2
Query: 21 WREWRAQFHRLFWAFYPCINGFKYCKPVVHVDGTWLYDKYKGTL 64
W++ FH FW F+ N +HV WL+ G +
Sbjct: 253 WKKVDVSFHSSFWPFFAHNN--------IHVKNEWLHHAGAGVI 146
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.138 0.452
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,109,991
Number of Sequences: 28460
Number of extensions: 137885
Number of successful extensions: 704
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 698
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 703
length of query: 440
length of database: 4,897,600
effective HSP length: 93
effective length of query: 347
effective length of database: 2,250,820
effective search space: 781034540
effective search space used: 781034540
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 57 (26.6 bits)
Medicago: description of AC126012.8