BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126012.10 + phase: 0 /pseudo
(498 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19053 similar to UP|Q9FHH9 (Q9FHH9) DNA-binding protein-like, ... 50 6e-07
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 35 0.028
AV408295 34 0.048
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 34 0.063
BP041267 32 0.18
AV420911 31 0.41
TC18927 similar to PIR|AI2934|AI2934 chromate transport protein ... 31 0.53
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 31 0.53
TC12104 30 0.70
TC19433 30 0.91
TC20112 weakly similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich... 29 1.6
TC20061 29 1.6
TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, part... 29 2.0
TC8052 similar to GB|AAP13431.1|30023796|BT006323 At5g02530 {Ara... 29 2.0
BP065763 29 2.0
TC14940 28 2.6
TC12900 28 2.6
CN825282 28 3.5
TC9979 similar to UP|HIR5_MOUSE (Q9QZ23) HIRA-interacting protei... 28 4.5
CB826810 28 4.5
>TC19053 similar to UP|Q9FHH9 (Q9FHH9) DNA-binding protein-like, partial
(3%)
Length = 603
Score = 50.4 bits (119), Expect = 6e-07
Identities = 30/51 (58%), Positives = 37/51 (71%), Gaps = 1/51 (1%)
Frame = +1
Query: 449 RDFAEFNIGMNVPPPVMNMNREPVMNKNREDFEARNAIL-KKQENERRVER 498
RD A+F++GMNVPPP PVM +RE+FEAR A L +K+ENERR ER
Sbjct: 4 RDLADFSMGMNVPPP------PPVM--SREEFEARKADLRRKRENERRGER 132
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 35.0 bits (79), Expect = 0.028
Identities = 25/69 (36%), Positives = 30/69 (43%)
Frame = +1
Query: 65 GAGRGFRRGAVGGRIGGGRGFGLERKTPPEGYVCHRCKVSGHFIQHCPTNGDSTFDIKKV 124
G G G G GGR GGG G G + K C+ C GHF + C G S ++
Sbjct: 304 GGGGGGGGGRGGGRSGGGGG-GSDMK-------CYECGEPGHFARECRMRGGS--GRRRS 453
Query: 125 RQPTGIPRS 133
R P RS
Sbjct: 454 RSPPRFRRS 480
>AV408295
Length = 426
Score = 34.3 bits (77), Expect = 0.048
Identities = 35/144 (24%), Positives = 60/144 (41%)
Frame = +3
Query: 223 TNILADDLLPNKTLRDAIHRILESGNSSTENAGSTYQVQDMLSSRCPQPKIPSPTSSATS 282
+N++AD P K D + ++++ + E S P PK PSP++ A
Sbjct: 12 SNVVADLPEPQKKALDDLKQLIQDALNKHE------------LSAPPPPKAPSPSTEA-K 152
Query: 283 KGEPKVSQVNEGMANIQEIVAERKEVSATQQVSEQVKIPRAAVVSEVTHESMSVKEPEPA 342
K E + E + +E E ++ A ++ E+V E E + E E
Sbjct: 153 KDEVAAAATEEKVEEEEEKKTEEEKKPAEEEKKEEVVEEEKKPAEE---EKKAEAEGEAT 323
Query: 343 SQGSAKLVEEEVQQKLVPTDGGKK 366
+ G K V E V + +V + G+K
Sbjct: 324 TSGEEKNVVEAVTEVVV--EAGEK 389
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 33.9 bits (76), Expect = 0.063
Identities = 19/46 (41%), Positives = 23/46 (49%)
Frame = +1
Query: 72 RGAVGGRIGGGRGFGLERKTPPEGYVCHRCKVSGHFIQHCPTNGDS 117
+G GGR GGGRG G E C+ C GHF + C + G S
Sbjct: 109 KGGGGGR-GGGRGRG------GEDLKCYECGEPGHFARECRSRGGS 225
>BP041267
Length = 520
Score = 32.3 bits (72), Expect = 0.18
Identities = 24/88 (27%), Positives = 43/88 (48%), Gaps = 7/88 (7%)
Frame = +2
Query: 275 SPTSSATSKGEPKVSQVNEGMANIQEIVAERKEVSATQQVSEQVKIPRAAVVSEVTHESM 334
SPT AT + V+ N+ N+Q+ + E+++ V E+ K AAVV++ T +
Sbjct: 32 SPTIVATKETHSNVATTNDVEGNVQKSIDEKRQ----DVVHEEQKEKEAAVVTDNTERAK 199
Query: 335 S-------VKEPEPASQGSAKLVEEEVQ 355
S V E + + + +VE+ V+
Sbjct: 200 SSDDVRDEVVEVKVLEENNVAVVEKNVE 283
>AV420911
Length = 418
Score = 31.2 bits (69), Expect = 0.41
Identities = 27/73 (36%), Positives = 31/73 (41%), Gaps = 4/73 (5%)
Frame = +2
Query: 43 DSKIKALIDTPALD----WQQQGSDFGAGRGFRRGAVGGRIGGGRGFGLERKTPPEGYVC 98
D K AL ALD W+ + S G G GGR GGGRG G E C
Sbjct: 236 DDKRDALDAIHALDGKNGWRVELSHNSKGGG------GGR-GGGRGRG------GEDLKC 376
Query: 99 HRCKVSGHFIQHC 111
+ C GHF + C
Sbjct: 377 YECGEPGHFAREC 415
>TC18927 similar to PIR|AI2934|AI2934 chromate transport protein chrA
[imported] - Agrobacterium tumefaciens
(strain C58, Dupont) {Agrobacterium tumefaciens;},
partial (6%)
Length = 561
Score = 30.8 bits (68), Expect = 0.53
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = -2
Query: 97 VCHRCKVSGHFIQHCP 112
VCHRC GHF CP
Sbjct: 332 VCHRCSKKGHFANRCP 285
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 30.8 bits (68), Expect = 0.53
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = +2
Query: 83 RGFGLERKTPPEGYVCHRCKVSGHFIQHCPTNGDS 117
RG+G R + G C +C GHF + CP+ G++
Sbjct: 380 RGYGGSRGS--NGGECFKCGKPGHFARECPSEGET 478
>TC12104
Length = 771
Score = 30.4 bits (67), Expect = 0.70
Identities = 12/49 (24%), Positives = 21/49 (42%)
Frame = +1
Query: 182 CPLCNNVMKDAVLTSKCCFKSFCDKCIRNYIMSKSACVCLATNILADDL 230
C +C M +L CC FC++C+ + + C ++ DL
Sbjct: 421 CAICQEKMHSPILL--CCKHMFCEECVSEWFERERTCPLCRASVKPADL 561
>TC19433
Length = 464
Score = 30.0 bits (66), Expect = 0.91
Identities = 20/54 (37%), Positives = 26/54 (48%)
Frame = -1
Query: 127 PTGIPRSMLMVNPQGSYALQNGSVAVLKPNEAAFDKEMEGLSSTRSVGDLPPEL 180
P G RS L+V + + KPNEAA++ EGL S R L PE+
Sbjct: 299 PNGSTRSALIVVCGEALHQRESGCFG*KPNEAAYELRTEGLDSGRRWEWLAPEM 138
>TC20112 weakly similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich
glycoprotein DZ-HRGP precursor, partial (11%)
Length = 523
Score = 29.3 bits (64), Expect = 1.6
Identities = 14/22 (63%), Positives = 14/22 (63%)
Frame = +2
Query: 61 GSDFGAGRGFRRGAVGGRIGGG 82
GS G GRG R G GGR GGG
Sbjct: 452 GSIRGGGRGGRGGGFGGRHGGG 517
>TC20061
Length = 490
Score = 29.3 bits (64), Expect = 1.6
Identities = 10/17 (58%), Positives = 12/17 (69%)
Frame = -2
Query: 95 GYVCHRCKVSGHFIQHC 111
GY H+C+V HFI HC
Sbjct: 273 GYRGHKCRVLSHFIYHC 223
>TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (56%)
Length = 598
Score = 28.9 bits (63), Expect = 2.0
Identities = 18/50 (36%), Positives = 21/50 (42%), Gaps = 3/50 (6%)
Frame = +1
Query: 65 GAGRGFRRGAVGGRIGGGRGFGLE---RKTPPEGYVCHRCKVSGHFIQHC 111
G RG R G GGGR E R PP C C + GH+ + C
Sbjct: 301 GVPRGSREG------GGGRDRDREYMGRGPPPGSGRCFNCGIDGHWARDC 432
>TC8052 similar to GB|AAP13431.1|30023796|BT006323 At5g02530 {Arabidopsis
thaliana;}, partial (55%)
Length = 1389
Score = 28.9 bits (63), Expect = 2.0
Identities = 16/26 (61%), Positives = 16/26 (61%)
Frame = +2
Query: 61 GSDFGAGRGFRRGAVGGRIGGGRGFG 86
G D G GRG RRG GR G GRG G
Sbjct: 746 GGDGGRGRGHRRGRGRGR-GHGRGRG 820
>BP065763
Length = 537
Score = 28.9 bits (63), Expect = 2.0
Identities = 9/17 (52%), Positives = 14/17 (81%)
Frame = -1
Query: 96 YVCHRCKVSGHFIQHCP 112
+VC+ CK +GHF ++CP
Sbjct: 387 HVCYFCKKAGHFKKYCP 337
>TC14940
Length = 781
Score = 28.5 bits (62), Expect = 2.6
Identities = 13/54 (24%), Positives = 24/54 (44%)
Frame = +2
Query: 178 PELHCPLCNNVMKDAVLTSKCCFKSFCDKCIRNYIMSKSACVCLATNILADDLL 231
P CP+C + + + T C FC CI+ I +++ C + +L+
Sbjct: 137 PVFSCPICMGPLVEEMSTR--CGHIFCKACIKAAISAQNKCPTCRKKVTVKELI 292
>TC12900
Length = 587
Score = 28.5 bits (62), Expect = 2.6
Identities = 18/60 (30%), Positives = 30/60 (50%)
Frame = +1
Query: 231 LPNKTLRDAIHRILESGNSSTENAGSTYQVQDMLSSRCPQPKIPSPTSSATSKGEPKVSQ 290
LP L A+H + + S T ++GS + + P PSPT A ++GE ++S+
Sbjct: 106 LPPPMLISALHSM--TATSLTGSSGSRLWMTRSRLALIPTDAPPSPTGLANARGEGRISR 279
>CN825282
Length = 678
Score = 28.1 bits (61), Expect = 3.5
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = +2
Query: 110 HCPTNGDSTFDIKKVRQPTGIPRSMLMVNPQGSYAL 145
H PT G + F ++ +P R+ +M+NP Y +
Sbjct: 500 HFPTEGKNKFQLRTNIEPLTFIRTKMMINPLVKYGI 607
>TC9979 similar to UP|HIR5_MOUSE (Q9QZ23) HIRA-interacting protein 5
(mHIRIP5), partial (36%)
Length = 1304
Score = 27.7 bits (60), Expect = 4.5
Identities = 17/44 (38%), Positives = 23/44 (51%)
Frame = +3
Query: 243 ILESGNSSTENAGSTYQVQDMLSSRCPQPKIPSPTSSATSKGEP 286
IL S N S+ + G D +SSR P PK+ P +S + K P
Sbjct: 312 ILPSLNHSSPSTGLLVSSLDRISSRLPNPKM-LPGNSLSLKSSP 440
>CB826810
Length = 603
Score = 27.7 bits (60), Expect = 4.5
Identities = 16/44 (36%), Positives = 21/44 (47%)
Frame = +2
Query: 265 SSRCPQPKIPSPTSSATSKGEPKVSQVNEGMANIQEIVAERKEV 308
S R P PK PSP +++ P+V+ A IV KEV
Sbjct: 440 SPRAPSPKAPSPRAASPRSPSPRVTSPK---AASSRIVHHHKEV 562
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.135 0.416
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,959,141
Number of Sequences: 28460
Number of extensions: 139645
Number of successful extensions: 1071
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 1025
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1061
length of query: 498
length of database: 4,897,600
effective HSP length: 94
effective length of query: 404
effective length of database: 2,222,360
effective search space: 897833440
effective search space used: 897833440
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC126012.10