BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126012.1 + phase: 0
(220 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14344 similar to UP|Q40310 (Q40310) Chalcone reductase, complete 263 2e-71
TC14345 homologue to UP|Q39774 (Q39774) Polyketide reductase, pa... 206 3e-54
TC8351 homologue to UP|Q41399 (Q41399) Chalcone reductase, complete 197 9e-52
TC9796 similar to UP|Q8S4C1 (Q8S4C1) Chalcone reductase, partial... 159 5e-40
BP053310 153 3e-38
TC19551 weakly similar to PIR|S57993|S57993 chalcone reductase h... 122 5e-29
TC13155 homologue to UP|Q9SXT0 (Q9SXT0) Chalcone reductase (Frag... 101 9e-23
BP068618 79 2e-20
AV769441 91 1e-19
TC9978 similar to UP|Q84TF0 (Q84TF0) At2g37790, partial (36%) 81 2e-16
TC15665 similar to PIR|T02543|T02543 aldehyde dehydrogenase homo... 72 1e-13
TC15536 similar to UP|Q9FVN7 (Q9FVN7) NADPH-dependent mannose 6-... 67 3e-12
BP063888 57 2e-09
TC16005 weakly similar to UP|Q96425 (Q96425) Polyketide reductas... 49 9e-07
TC18035 similar to UP|AAQ54521 (AAQ54521) Aldo/keto reductase (F... 47 2e-06
TC15046 similar to UP|Q9FVN7 (Q9FVN7) NADPH-dependent mannose 6-... 44 2e-05
TC18006 weakly similar to UP|Q9M338 (Q9M338) Reductase-like prot... 40 2e-04
TC14346 similar to UP|Q40310 (Q40310) Chalcone reductase, partia... 40 4e-04
BP055775 32 0.066
TC12794 homologue to UP|Q9SXF7 (Q9SXF7) Amino acid transporter-l... 29 0.56
>TC14344 similar to UP|Q40310 (Q40310) Chalcone reductase, complete
Length = 1180
Score = 263 bits (671), Expect = 2e-71
Identities = 128/148 (86%), Positives = 138/148 (92%)
Frame = +3
Query: 73 VSNFSVKKLENLLSVATILPAVNQVEMNLAWQQKKLREFCNANGIVLTAFSPLRKGASRG 132
VS S+ KL+NLLSVA ILPAVNQVEMNLAWQQK+LREFCNANGIVLTAFSPLRKG SRG
Sbjct: 570 VSATSLLKLQNLLSVANILPAVNQVEMNLAWQQKELREFCNANGIVLTAFSPLRKGGSRG 749
Query: 133 PNEVMENDMLKEIADAHGKSVAQISLRWLYEQGVTFVPKSYDKERMNQNLCIFDWSLTKE 192
PNEVMENDMLKEIA+AHGK++AQ+SLRWLYEQGVTF KSYDK+RMNQNL IFDW+LTKE
Sbjct: 750 PNEVMENDMLKEIAEAHGKTIAQVSLRWLYEQGVTFAAKSYDKDRMNQNLQIFDWALTKE 929
Query: 193 DHEKIDQIKQNRLIPGPTKPGINDLYDD 220
D EKIDQIKQNRLIPGPTKP +NDLYDD
Sbjct: 930 DLEKIDQIKQNRLIPGPTKPQLNDLYDD 1013
Score = 121 bits (304), Expect = 8e-29
Identities = 59/73 (80%), Positives = 65/73 (88%)
Frame = +2
Query: 13 SCRTLQLDYLDLYLIHWPLSSQPGKFTFPIDVADLLPFDVKGVWESMEEGLKLGLTKAIG 72
S +TLQL+YLDLYLIHWPLSS PGKF+FPI + DLLPFDVKGVWESMEE LKLGLTKAIG
Sbjct: 392 SLKTLQLEYLDLYLIHWPLSSTPGKFSFPIAMEDLLPFDVKGVWESMEECLKLGLTKAIG 571
Query: 73 VSNFSVKKLENLL 85
VSNFSVK ++ L
Sbjct: 572 VSNFSVKTSKSAL 610
>TC14345 homologue to UP|Q39774 (Q39774) Polyketide reductase, partial (35%)
Length = 516
Score = 206 bits (523), Expect = 3e-54
Identities = 98/112 (87%), Positives = 105/112 (93%)
Frame = +3
Query: 109 REFCNANGIVLTAFSPLRKGASRGPNEVMENDMLKEIADAHGKSVAQISLRWLYEQGVTF 168
REFC+ANGIVLTAFSPLRKG SRG NEVMENDMLKEIA+AHGKS+AQ+SLRWLYEQGVTF
Sbjct: 3 REFCSANGIVLTAFSPLRKGGSRGANEVMENDMLKEIAEAHGKSIAQVSLRWLYEQGVTF 182
Query: 169 VPKSYDKERMNQNLCIFDWSLTKEDHEKIDQIKQNRLIPGPTKPGINDLYDD 220
KSYDKERMNQNL IFDW+LTKED EKIDQIKQNRLIPGPTKP +NDL+DD
Sbjct: 183 AAKSYDKERMNQNLQIFDWALTKEDLEKIDQIKQNRLIPGPTKPQLNDLFDD 338
>TC8351 homologue to UP|Q41399 (Q41399) Chalcone reductase, complete
Length = 1331
Score = 197 bits (502), Expect = 9e-52
Identities = 104/199 (52%), Positives = 141/199 (70%), Gaps = 4/199 (2%)
Frame = +1
Query: 10 LLISCRTLQLDYLDLYLIHWPLSSQPG-KFTFPIDVADLLPFDVKGVWESMEEGLKLGLT 68
L + + L ++Y+DLYLIHWP+ + + DLLPFD++G W++MEE KLGL
Sbjct: 334 LKTTLKKLGVEYVDLYLIHWPVRLRHDLENPVVFSKEDLLPFDIEGTWKAMEECYKLGLA 513
Query: 69 KAIGVSNFSVKKLENLLSVATILPAVNQVEMNLAWQQKKLREFCNANGIVLTAFSPLRKG 128
K+IG+ NF KKL LL +ATI PAVNQVEMN +WQQ KLREFC GI ++A+S L G
Sbjct: 514 KSIGICNFGTKKLTKLLEIATITPAVNQVEMNPSWQQGKLREFCKQKGIHVSAWSAL--G 687
Query: 129 ASR---GPNEVMENDMLKEIADAHGKSVAQISLRWLYEQGVTFVPKSYDKERMNQNLCIF 185
A + G VMEN +L++IA A GK+VAQ++LRW+Y+QG + + KS++KERM QNL IF
Sbjct: 688 AYKVTWGSGAVMENHILQDIATAKGKTVAQVALRWVYQQGSSAMAKSFNKERMKQNLEIF 867
Query: 186 DWSLTKEDHEKIDQIKQNR 204
D+ L++E+ EKI Q+ Q R
Sbjct: 868 DFELSEEELEKIKQVPQRR 924
>TC9796 similar to UP|Q8S4C1 (Q8S4C1) Chalcone reductase, partial (32%)
Length = 497
Score = 159 bits (401), Expect = 5e-40
Identities = 74/100 (74%), Positives = 90/100 (90%)
Frame = +2
Query: 121 AFSPLRKGASRGPNEVMENDMLKEIADAHGKSVAQISLRWLYEQGVTFVPKSYDKERMNQ 180
AFSPLRKGAS G N VM+ND+LKE+ADAHG+++AQI LRWLYEQG+TFV KSYDKERMNQ
Sbjct: 2 AFSPLRKGASTGENLVMDNDILKELADAHGQTIAQICLRWLYEQGLTFVAKSYDKERMNQ 181
Query: 181 NLCIFDWSLTKEDHEKIDQIKQNRLIPGPTKPGINDLYDD 220
NL IFDWSLT++D++KI +I Q+RLI GPTKP +NDL+D+
Sbjct: 182 NLQIFDWSLTEDDYKKISEIHQDRLIKGPTKPLLNDLWDE 301
>BP053310
Length = 473
Score = 153 bits (386), Expect = 3e-38
Identities = 72/89 (80%), Positives = 80/89 (88%)
Frame = -2
Query: 132 GPNEVMENDMLKEIADAHGKSVAQISLRWLYEQGVTFVPKSYDKERMNQNLCIFDWSLTK 191
GPNEVMENDMLKEI +AHGKS+AQ+SLRWLYEQGVTFV KSYDK+ MN NL IFDW+LT
Sbjct: 472 GPNEVMENDMLKEIVEAHGKSIAQVSLRWLYEQGVTFVAKSYDKDIMNXNLQIFDWALTX 293
Query: 192 EDHEKIDQIKQNRLIPGPTKPGINDLYDD 220
ED EKIDQI+ NRLIPGPTKP +NDL+DD
Sbjct: 292 EDLEKIDQIE*NRLIPGPTKPRLNDLFDD 206
>TC19551 weakly similar to PIR|S57993|S57993 chalcone reductase homolog -
Sesbania rostrata (fragment) {Sesbania rostrata;},
partial (81%)
Length = 624
Score = 122 bits (306), Expect = 5e-29
Identities = 63/122 (51%), Positives = 86/122 (69%), Gaps = 1/122 (0%)
Frame = +2
Query: 88 ATILPAVNQVEMNLAWQQKKLREFCNANGIVLTAFSPL-RKGASRGPNEVMENDMLKEIA 146
ATI PA+ QVE+N AWQQ+ LR FC GI ++A+SPL GA G VM++ +LK+IA
Sbjct: 8 ATIPPALVQVELNAAWQQENLRRFCKEKGIHVSAWSPLGANGAMWGSLAVMDSPILKDIA 187
Query: 147 DAHGKSVAQISLRWLYEQGVTFVPKSYDKERMNQNLCIFDWSLTKEDHEKIDQIKQNRLI 206
K+VAQ++LRW+ EQG+T + +S++KERM NL IFDW L++ D EKI Q+ Q+R
Sbjct: 188 ITTEKTVAQVALRWIIEQGITPIVRSFNKERMKHNLEIFDWELSENDLEKIKQMPQHRAF 367
Query: 207 PG 208
G
Sbjct: 368 SG 373
>TC13155 homologue to UP|Q9SXT0 (Q9SXT0) Chalcone reductase (Fragment),
partial (76%)
Length = 392
Score = 101 bits (252), Expect = 9e-23
Identities = 45/62 (72%), Positives = 56/62 (89%)
Frame = +3
Query: 159 RWLYEQGVTFVPKSYDKERMNQNLCIFDWSLTKEDHEKIDQIKQNRLIPGPTKPGINDLY 218
RWLYEQG+TFV +SYDKERMNQNL IFDWSLT++D++KI +I Q+RLI GPTKP +NDL+
Sbjct: 3 RWLYEQGLTFVAQSYDKERMNQNLQIFDWSLTEDDYKKISEIHQDRLIKGPTKPLLNDLW 182
Query: 219 DD 220
D+
Sbjct: 183 DE 188
>BP068618
Length = 420
Score = 79.3 bits (194), Expect(3) = 2e-20
Identities = 37/47 (78%), Positives = 40/47 (84%)
Frame = -3
Query: 159 RWLYEQGVTFVPKSYDKERMNQNLCIFDWSLTKEDHEKIDQIKQNRL 205
RWLYEQG+TF KSYDKERMN NL IFDW+LT ED EKIDQI+QN L
Sbjct: 352 RWLYEQGLTFPAKSYDKERMNPNLHIFDWALTNEDLEKIDQIQQNPL 212
Score = 31.2 bits (69), Expect(3) = 2e-20
Identities = 13/14 (92%), Positives = 14/14 (99%)
Frame = -2
Query: 137 MENDMLKEIADAHG 150
MENDMLKEIA+AHG
Sbjct: 419 MENDMLKEIAEAHG 378
Score = 23.5 bits (49), Expect(3) = 2e-20
Identities = 11/23 (47%), Positives = 15/23 (64%), Gaps = 2/23 (8%)
Frame = -1
Query: 200 IKQNRLI--PGPTKPGINDLYDD 220
IK +R + PGP P +DL+DD
Sbjct: 234 IKSSRTL*LPGPPTPPPHDLFDD 166
>AV769441
Length = 594
Score = 91.3 bits (225), Expect = 1e-19
Identities = 47/86 (54%), Positives = 58/86 (66%)
Frame = +3
Query: 15 RTLQLDYLDLYLIHWPLSSQPGKFTFPIDVADLLPFDVKGVWESMEEGLKLGLTKAIGVS 74
R LQLDYLDLYLIHWP+S + G+ T P D+ W +ME G +AIGVS
Sbjct: 354 RDLQLDYLDLYLIHWPVSMKNGQLTKP---------DIPSTWRAMEALYNSGKARAIGVS 506
Query: 75 NFSVKKLENLLSVATILPAVNQVEMN 100
NFSVKKL++LL VA + PAVNQVE++
Sbjct: 507 NFSVKKLQDLLEVANVPPAVNQVELH 584
>TC9978 similar to UP|Q84TF0 (Q84TF0) At2g37790, partial (36%)
Length = 813
Score = 80.9 bits (198), Expect = 2e-16
Identities = 38/102 (37%), Positives = 64/102 (62%)
Frame = +1
Query: 107 KLREFCNANGIVLTAFSPLRKGASRGPNEVMENDMLKEIADAHGKSVAQISLRWLYEQGV 166
KL FC + G+ L+ +SPL KG + +++N +L IA+ GK+ AQI+LRW + G
Sbjct: 4 KLHAFCKSKGVHLSGYSPLGKGLE---SNILKNQVLHTIAEKLGKTPAQIALRWGLQMGH 174
Query: 167 TFVPKSYDKERMNQNLCIFDWSLTKEDHEKIDQIKQNRLIPG 208
+ +PKS + R+ +N+ +FDWS+ + +I+Q R++PG
Sbjct: 175 SVLPKSTNATRIKENIDLFDWSIPADLLANFSEIEQERIVPG 300
>TC15665 similar to PIR|T02543|T02543 aldehyde dehydrogenase homolog
At2g37770 - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (59%)
Length = 582
Score = 71.6 bits (174), Expect = 1e-13
Identities = 40/89 (44%), Positives = 53/89 (58%)
Frame = +1
Query: 2 IKINFDLDLLISCRTLQLDYLDLYLIHWPLSSQPGKFTFPIDVADLLPFDVKGVWESMEE 61
+ I FD L + LQLDY+DLYLIHWP+ ++ G F + D D+ W +ME
Sbjct: 331 VPIAFDRTL----QDLQLDYIDLYLIHWPIRTKKGAVGFKPENLDQP--DIPSTWRAMEA 492
Query: 62 GLKLGLTKAIGVSNFSVKKLENLLSVATI 90
G KAIGVSNFS KKL++LL +A +
Sbjct: 493 LFDSGKAKAIGVSNFSSKKLQDLLEIARV 579
>TC15536 similar to UP|Q9FVN7 (Q9FVN7) NADPH-dependent mannose 6-phosphate
reductase, partial (40%)
Length = 642
Score = 66.6 bits (161), Expect = 3e-12
Identities = 34/102 (33%), Positives = 60/102 (58%), Gaps = 4/102 (3%)
Frame = +2
Query: 103 WQQKKLREFCNANGIVLTAFSPLRKGASR----GPNEVMENDMLKEIADAHGKSVAQISL 158
+Q+ L +FC +G+ +TA +PL A+ G +++ +LK ++ + K+ AQI+L
Sbjct: 14 FQRDNLVKFCQKHGVSVTAHTPLGGAAANTEWFGSVSCLDDQVLKGLSAKYKKTTAQIAL 193
Query: 159 RWLYEQGVTFVPKSYDKERMNQNLCIFDWSLTKEDHEKIDQI 200
RW ++ +PK+ ER+ +N +FD+ L+KED E I I
Sbjct: 194 RWGIQRNTVVIPKTSKLERLKENFDVFDFELSKEDMELIGSI 319
>BP063888
Length = 456
Score = 57.0 bits (136), Expect = 2e-09
Identities = 29/96 (30%), Positives = 56/96 (58%)
Frame = -3
Query: 111 FCNANGIVLTAFSPLRKGASRGPNEVMENDMLKEIADAHGKSVAQISLRWLYEQGVTFVP 170
FC + G+ ++ +SPL A +++++ + +A+ GK+ AQ+ LRW + G + +P
Sbjct: 454 FCXSKGVHVSGYSPLGSPAWLD-GDILKHPNITMVAEKLGKTPAQVVLRWGLQVGHSVLP 278
Query: 171 KSYDKERMNQNLCIFDWSLTKEDHEKIDQIKQNRLI 206
KS + R+ +N I +WS+ ++ K +I Q RL+
Sbjct: 277 KSSNLARIKENFDILEWSIPEDLLPKFSEIPQARLL 170
>TC16005 weakly similar to UP|Q96425 (Q96425) Polyketide reductase (GGPKR1),
partial (40%)
Length = 474
Score = 48.5 bits (114), Expect = 9e-07
Identities = 19/29 (65%), Positives = 25/29 (85%)
Frame = +1
Query: 13 SCRTLQLDYLDLYLIHWPLSSQPGKFTFP 41
S RTLQL+YLDL+LIHWP+++QPG +P
Sbjct: 388 SLRTLQLEYLDLFLIHWPITTQPGGVKYP 474
>TC18035 similar to UP|AAQ54521 (AAQ54521) Aldo/keto reductase (Fragment),
partial (43%)
Length = 550
Score = 47.4 bits (111), Expect = 2e-06
Identities = 21/59 (35%), Positives = 39/59 (65%)
Frame = +3
Query: 150 GKSVAQISLRWLYEQGVTFVPKSYDKERMNQNLCIFDWSLTKEDHEKIDQIKQNRLIPG 208
GK+ AQ++LRW ++G + +P S ++ R+ N +FDW++ ++ K +I+Q RL+ G
Sbjct: 12 GKTPAQVALRWGLQKGNSVLP*STNEARIKLNFDVFDWTIPEDLLAKFSEIEQARLLRG 188
>TC15046 similar to UP|Q9FVN7 (Q9FVN7) NADPH-dependent mannose 6-phosphate
reductase, partial (54%)
Length = 547
Score = 44.3 bits (103), Expect = 2e-05
Identities = 24/73 (32%), Positives = 41/73 (55%), Gaps = 7/73 (9%)
Frame = +1
Query: 13 SCRTLQLDYLDLYLIHWPLSSQ------PGKFTFPIDVADL-LPFDVKGVWESMEEGLKL 65
S + LQLDYLDLYL+H+P++++ V D+ ++ W +ME +
Sbjct: 322 SLKKLQLDYLDLYLVHFPVATRHTGVGTTDSVKGEDGVLDIDTTISLETTWHAMEGLVSS 501
Query: 66 GLTKAIGVSNFSV 78
GL ++IG+SN+ +
Sbjct: 502 GLVRSIGISNYDI 540
>TC18006 weakly similar to UP|Q9M338 (Q9M338) Reductase-like protein,
partial (21%)
Length = 481
Score = 40.4 bits (93), Expect = 2e-04
Identities = 19/44 (43%), Positives = 27/44 (61%)
Frame = +1
Query: 165 GVTFVPKSYDKERMNQNLCIFDWSLTKEDHEKIDQIKQNRLIPG 208
G + +PKS + R+ QN +FDWS+ + K +IKQ RLI G
Sbjct: 7 GHSVLPKSTTEVRIKQNFDVFDWSIPEGLFAKFSEIKQERLIKG 138
>TC14346 similar to UP|Q40310 (Q40310) Chalcone reductase, partial (38%)
Length = 435
Score = 39.7 bits (91), Expect = 4e-04
Identities = 16/19 (84%), Positives = 18/19 (94%)
Frame = +2
Query: 13 SCRTLQLDYLDLYLIHWPL 31
S +TLQL+YLDLYLIHWPL
Sbjct: 377 SLKTLQLEYLDLYLIHWPL 433
>BP055775
Length = 485
Score = 32.3 bits (72), Expect = 0.066
Identities = 15/59 (25%), Positives = 36/59 (60%), Gaps = 2/59 (3%)
Frame = -3
Query: 142 LKEIADAHGKSVAQISLRWLYEQGVTFVP--KSYDKERMNQNLCIFDWSLTKEDHEKID 198
L ++A+ HG + +Q++L W++ QG VP + + ++ N+ + L+++D ++I+
Sbjct: 468 LVKLAEKHGCTSSQLALAWMFHQGDDVVPIXGTTKIKNLDSNISSLEVKLSEDDLKEIE 292
>TC12794 homologue to UP|Q9SXF7 (Q9SXF7) Amino acid transporter-like protein
1, partial (19%)
Length = 679
Score = 29.3 bits (64), Expect = 0.56
Identities = 16/50 (32%), Positives = 22/50 (44%), Gaps = 9/50 (18%)
Frame = +1
Query: 29 WPLSSQPGKFTFP---------IDVADLLPFDVKGVWESMEEGLKLGLTK 69
W L QP K++F + V L F + G+W + GLKL K
Sbjct: 142 WVLIKQPTKYSFNWYFNWILGWLGVGFSLAFSIGGIWSMVNNGLKLKFFK 291
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.138 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,844,678
Number of Sequences: 28460
Number of extensions: 48982
Number of successful extensions: 258
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 250
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 250
length of query: 220
length of database: 4,897,600
effective HSP length: 87
effective length of query: 133
effective length of database: 2,421,580
effective search space: 322070140
effective search space used: 322070140
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)
Medicago: description of AC126012.1