BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC125481.2 + phase: 0 /pseudo
(1546 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG662087 149 3e-36
TC18698 66 5e-11
BP030435 59 6e-09
TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase regi... 57 2e-08
TC16320 weakly similar to UP|Q84TE0 (Q84TE0) At5g51080, partial ... 46 5e-05
TC16930 44 3e-04
AV410603 39 0.008
BF177840 31 1.3
TC18092 31 1.7
BP080049 30 2.2
>BG662087
Length = 373
Score = 149 bits (376), Expect = 3e-36
Identities = 68/121 (56%), Positives = 89/121 (73%)
Frame = +1
Query: 645 SSGKWRMCVDYTDLNMACPKDPYPLPNIDHLIDNAAGYKTLSFMDAYSDYNQMKMDLLDA 704
+SGKWRM VDYTDLN ACPKD YPLP+ID L+D A+ + LS MDAYS Y+Q+KM D
Sbjct: 10 TSGKWRMWVDYTDLNKACPKDSYPLPSIDKLVDGASDNELLSLMDAYSGYHQIKMHPSDE 189
Query: 705 PKIVFMTNQKNYHYEVMSFGLRNAGATFQRSMDTIFSAQIGRNLEVYVDDLVVKTSAEGQ 764
K FMT + NY Y+ + FGL+NAGAT+Q MD +F +GRN+EVY+D+++VK++
Sbjct: 190 DKTAFMTARVNYCYQTIPFGLKNAGATYQXLMDRVFXDXVGRNMEVYLDNMIVKSALRAN 369
Query: 765 H 765
H
Sbjct: 370 H 372
>TC18698
Length = 808
Score = 65.9 bits (159), Expect = 5e-11
Identities = 32/64 (50%), Positives = 43/64 (67%)
Frame = -2
Query: 706 KIVFMTNQKNYHYEVMSFGLRNAGATFQRSMDTIFSAQIGRNLEVYVDDLVVKTSAEGQH 765
K N+ NY+Y+VM GL+N T+QR MD IF QI +N+EVYV+D++VK+S E H
Sbjct: 801 KTTLKINRVNYYYQVMPLGLKNI*TTYQRLMDKIFHKQI*KNVEVYVEDMIVKSSQE*FH 622
Query: 766 SEDL 769
DL
Sbjct: 621 RGDL 610
>BP030435
Length = 533
Score = 58.9 bits (141), Expect = 6e-09
Identities = 25/61 (40%), Positives = 43/61 (69%)
Frame = +1
Query: 342 ISFSQEDFRQVIPHDDDPLVVSFQLLNWEIKRVLVDTGSSANVLYYDAFSKMGLSEEQLQ 401
I+FS +DF ++ PH D+P+V+ ++ ++RVL+D GSSA+++Y F ++GL+E L
Sbjct: 349 ITFSSDDFIRIKPHLDNPIVILLRVNQLNVQRVLLDQGSSADIIYGGVFDRLGLNEADLT 528
Query: 402 P 402
P
Sbjct: 529 P 531
>TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase region
(Fragment), partial (10%)
Length = 572
Score = 57.0 bits (136), Expect = 2e-08
Identities = 27/60 (45%), Positives = 37/60 (61%)
Frame = +2
Query: 1485 VLWSYHTTPHSTTGETPFTMVYGADAMLPVEIDTPTWRRENFNEEANEVGIQCTMDMIDE 1544
VLWSY+T P S+ ETPF + YGAD MLPVEI +W + +EE N + + + + E
Sbjct: 200 VLWSYNTMPQSSIKETPF*LTYGADTMLPVEISNFSW*TKARHEEENRMNMAVGWEKLPE 379
Score = 31.2 bits (69), Expect = 1.3
Identities = 20/57 (35%), Positives = 29/57 (50%)
Frame = +3
Query: 1378 MGLSSPVQESSTSANNWELKQSSYQ*LTLKQMDSQKPPTK*F*MV*KRSLRQPKDSG 1434
M +SP + A WE + S+Q* LK M Q+ T+*F* *+ + K+ G
Sbjct: 12 MEFNSPANKLRIFAMAWEFR*GSHQ*SILKLMGRQRLRTR*F*RA*RGGYMKLKEDG 182
>TC16320 weakly similar to UP|Q84TE0 (Q84TE0) At5g51080, partial (11%)
Length = 632
Score = 45.8 bits (107), Expect = 5e-05
Identities = 24/80 (30%), Positives = 41/80 (51%)
Frame = +2
Query: 1090 RSDSQLMTNQISGEYQTKDSQLSKYLSSVRNLAAFFNFFEVIYVPREQNVRADLLAKLAS 1149
+ DS L+ NQ+ G ++ K+ ++ S + L F F++ ++PRE N AD+ A
Sbjct: 65 KGDSMLVCNQVQGLWKIKNQNIASLCSEAKELKNKFLSFKINHIPREYNSEADVQANFGI 244
Query: 1150 TKRPGNNRTVIQEVVSAPNC 1169
+ R G ++EV P C
Sbjct: 245 SLRAGQ----VEEVREVP*C 292
>TC16930
Length = 520
Score = 43.5 bits (101), Expect = 3e-04
Identities = 22/68 (32%), Positives = 39/68 (57%)
Frame = +1
Query: 440 YLVFNAPSSYNAIIGRPSINLLDAFVSTKHLMMKYPLDNGRVGIVRGD*KIAREYYHASL 499
+++ N +SYNAI+ PS+N+ D + T+ L K+ DN + ++GD ++A Y S+
Sbjct: 10 FMLANNLTSYNAILC*PSLNVFDMCILTRRLASKFSTDNTEIVTIQGDQEVAPNCYIMSM 189
Query: 500 KLNVKSSE 507
+ S E
Sbjct: 190 WIIPDSQE 213
>AV410603
Length = 162
Score = 38.5 bits (88), Expect = 0.008
Identities = 18/49 (36%), Positives = 28/49 (56%)
Frame = +1
Query: 664 KDPYPLPNIDHLIDNAAGYKTLSFMDAYSDYNQMKMDLLDAPKIVFMTN 712
KD +P+P +D L+D G + S +D S Y+Q+ + D K VF T+
Sbjct: 10 KDSFPMPTVDELLDELRGSQFFSKLDLRSGYHQILVKPEDRHKTVFRTH 156
>BF177840
Length = 410
Score = 31.2 bits (69), Expect = 1.3
Identities = 11/30 (36%), Positives = 21/30 (69%)
Frame = +2
Query: 1487 WSYHTTPHSTTGETPFTMVYGADAMLPVEI 1516
++Y+ HSTT +PF +VYG + + P+++
Sbjct: 224 FAYNRVVHSTTKHSPFEIVYGYNPLTPLDL 313
>TC18092
Length = 519
Score = 30.8 bits (68), Expect = 1.7
Identities = 15/35 (42%), Positives = 23/35 (64%)
Frame = -2
Query: 651 MCVDYTDLNMACPKDPYPLPNIDHLIDNAAGYKTL 685
MCV TD+N CP+ PL +I+ L+D ++ Y+ L
Sbjct: 101 MCVC*TDINKDCPR--XPLTSINALVDYSSSYEYL 3
>BP080049
Length = 356
Score = 30.4 bits (67), Expect = 2.2
Identities = 15/39 (38%), Positives = 22/39 (55%)
Frame = -1
Query: 1109 SQLSKYLSSVRNLAAFFNFFEVIYVPREQNVRADLLAKL 1147
S L + RNL ++F+ F++ +V R N D LAKL
Sbjct: 245 SVLDTLILDCRNLLSYFDVFQLSFVRRTSNCVVDALAKL 129
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.339 0.147 0.477
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,617,886
Number of Sequences: 28460
Number of extensions: 351426
Number of successful extensions: 2506
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 2476
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2506
length of query: 1546
length of database: 4,897,600
effective HSP length: 102
effective length of query: 1444
effective length of database: 1,994,680
effective search space: 2880317920
effective search space used: 2880317920
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.9 bits)
S2: 61 (28.1 bits)
Medicago: description of AC125481.2