BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC125478.9 + phase: 0
(477 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11221 similar to UP|SQD1_SPIOL (Q84KI6) UDP-sulfoquinovose syn... 226 6e-60
TC10156 similar to UP|SQD1_ARATH (O48917) UDP-sulfoquinovose syn... 186 9e-48
TC8417 similar to UP|GAE1_PEA (Q43070) UDP-glucose 4-epimerase ... 51 5e-07
TC14852 similar to UP|Q9LZI2 (Q9LZI2) DTDP-glucose 4-6-dehydrata... 39 0.002
TC7971 homologue to PIR|C86216|C86216 protein T23G18.6 [imported... 36 0.016
TC14436 homologue to UP|Q9AV98 (Q9AV98) UDP-D-glucuronate carbox... 35 0.035
TC9292 homologue to UP|AAR07600 (AAR07600) Fiber dTDP-glucose 4-... 35 0.035
AV768744 33 0.10
TC16761 similar to GB|AAL32703.1|17065098|AY062625 nucleotide su... 31 0.51
TC8032 similar to UP|Q9M0B6 (Q9M0B6) Nucleotide sugar epimerase-... 30 0.66
AV406605 29 1.9
TC13206 homologue to UP|Q9AV98 (Q9AV98) UDP-D-glucuronate carbox... 28 3.3
TC8795 similar to UP|GMD1_ARATH (Q9SNY3) GDP-mannose 4,6 dehydra... 28 4.3
TC15725 similar to UP|MDHG_ARATH (Q9ZP05) Malate dehydrogenase, ... 27 5.6
TC16345 weakly similar to UP|Q8RX71 (Q8RX71) At3g51780/ORF3, par... 27 7.3
TC14616 27 7.3
AV428492 27 9.5
TC8094 homologue to UP|EFTU_PEA (O24310) Elongation factor Tu, c... 27 9.5
>TC11221 similar to UP|SQD1_SPIOL (Q84KI6) UDP-sulfoquinovose synthase,
chloroplast precursor (Sulfite:UDP-glucose
sulfotransferase) (Sulfolipid biosynthesis protein)
(SoSQD1) , partial (12%)
Length = 570
Score = 226 bits (576), Expect = 6e-60
Identities = 110/141 (78%), Positives = 124/141 (87%)
Frame = +2
Query: 1 MAQLLSSSCSLTFSASNKPCLKPFHQCSTSFSNTVVCDNSKTPFRQLFLREQKPRKSLAV 60
MAQLLSSSCSLT S+ NKP LKP +QCST FS V C+NS++P R+L LR+QKPRKS +
Sbjct: 140 MAQLLSSSCSLTISSRNKPYLKPLNQCSTPFSVMVGCNNSRSPLRRLSLRDQKPRKSFSA 319
Query: 61 VNASTISTGQEAPVQTSSGDPFKPKRVMVIGGDGYCGWATALHLSNKGYEVAIVDNLVRR 120
V+A+ +ST Q+ PVQTSSGDPFK KRVMV GGDGYCGWATALHLSNKGYEVAIVDNLVRR
Sbjct: 320 VHAAAVSTSQDTPVQTSSGDPFKAKRVMVFGGDGYCGWATALHLSNKGYEVAIVDNLVRR 499
Query: 121 LFDHQLGLDSLTPISSIQDRI 141
LFDHQLGLDSLTPISSIQ+R+
Sbjct: 500 LFDHQLGLDSLTPISSIQNRL 562
>TC10156 similar to UP|SQD1_ARATH (O48917) UDP-sulfoquinovose synthase,
chloroplast precursor (Sulfite:UDP-glucose
sulfotransferase) (Sulfolipid biosynthesis protein) ,
partial (20%)
Length = 515
Score = 186 bits (471), Expect = 9e-48
Identities = 89/96 (92%), Positives = 94/96 (97%)
Frame = +2
Query: 380 QFKVTELAELVTKAGEKLGLDVKTISVPNPRVELEEHYYNCKNTKLVDLGLKPHFLSDSL 439
QFKV+ELA LVTKAGEKLGLDVKT+SVPNPRVELEEHYYNCKNTKL+DLGLKPHFLSDSL
Sbjct: 2 QFKVSELANLVTKAGEKLGLDVKTMSVPNPRVELEEHYYNCKNTKLIDLGLKPHFLSDSL 181
Query: 440 IDSLLNFAVQYKDRVDTKQIMPGVSWRKVGVKTKTL 475
+DSLLNF VQYKDRVDTKQIMPGVSWRKVGVKTKT+
Sbjct: 182 LDSLLNFTVQYKDRVDTKQIMPGVSWRKVGVKTKTV 289
>TC8417 similar to UP|GAE1_PEA (Q43070) UDP-glucose 4-epimerase
(Galactowaldenase) (UDP-galactose 4-epimerase) , partial
(65%)
Length = 789
Score = 50.8 bits (120), Expect = 5e-07
Identities = 41/162 (25%), Positives = 79/162 (48%)
Frame = +3
Query: 56 KSLAVVNASTISTGQEAPVQTSSGDPFKPKRVMVIGGDGYCGWATALHLSNKGYEVAIVD 115
+SL ++ +S V +SS ++++V GG G+ G T + L + G+ V+I+D
Sbjct: 30 RSL*ILTHQILSPNSNKMVSSSSPQ----QKILVTGGAGFIGTHTVVQLLHDGFHVSIID 197
Query: 116 NLVRRLFDHQLGLDSLTPISSIQDRIQCWKSLTGKSIELYIGDICEFEFLSETFKSYEPD 175
N FD+ + + +++ + S K+++ GD+ + L E F + D
Sbjct: 198 N-----FDNSC----MEAVDRVREVVGPHLS---KNLQFTQGDLRNKDDLEEIFSKTKFD 341
Query: 176 AVVHFGEQRSAPYSMIDRSRAVYTQQNNVVGTLNVLFAIKEY 217
AV+HF ++ S+ + R N+VGT+N+ A+ +Y
Sbjct: 342 AVIHFAGLKAVGESVANPRRYF---DFNLVGTINLYQAMAKY 458
>TC14852 similar to UP|Q9LZI2 (Q9LZI2) DTDP-glucose 4-6-dehydratase homolog
D18 (At3g62830/F26K9_260), partial (70%)
Length = 1470
Score = 38.9 bits (89), Expect = 0.002
Identities = 69/283 (24%), Positives = 100/283 (34%), Gaps = 9/283 (3%)
Frame = +3
Query: 83 KPKRVMVIGGDGYCGWATALHLSNKGYEVAIVDNLV---RRLFDHQLGLDSLTPISSIQD 139
K R++V GG G+ G L +G V +VDN + H G
Sbjct: 753 KGLRIVVTGGAGFVGSHLVDRLMARGDSVIVVDNFFTGRKENVMHHFG------------ 896
Query: 140 RIQCWKSLTGKSIELYIGDICEFEFLSETFKSYEPDAVVHFGEQRSAPYSMID-RSRAVY 198
EL D+ E L E D + H + P S + + V
Sbjct: 897 ---------NPRFELIRHDVVEPLLL-------EVDQIYHL----ACPASPVHYKFNPVK 1016
Query: 199 TQQNNVVGTLNVLFAIKEYREDCHLVKLGTMGEYGTPNIDIEEGYITITHNGRTDTLPYP 258
T + NVVGTLN+L K R + T YG P L +P
Sbjct: 1017TIKTNVVGTLNMLGLAK--RVGARFLLTSTSEVYGDP-------------------LQHP 1133
Query: 259 KQASSFYHLSKVHDSHNIAFTCKAWGIRAT-----DLNQGVVYGVRTDETAMHEELCNRF 313
++ + + +++ + +C G R D ++G GV + R
Sbjct: 1134QKETYWGNVNPIG-----VRSCYDEGKRTAETLTMDYHRGA--GVEVRIARIFNTYGPRM 1292
Query: 314 DYDAIFGTALNRFCVQAAVGHPLTVYGKGGQTRAFLDIRDTVQ 356
D G + F QA PLTVYG G QTR+F + D V+
Sbjct: 1293CLDD--GRVASNFDAQALTKEPLTVYGDGKQTRSFQYVSDLVE 1415
>TC7971 homologue to PIR|C86216|C86216 protein T23G18.6 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(98%)
Length = 1662
Score = 35.8 bits (81), Expect = 0.016
Identities = 36/126 (28%), Positives = 56/126 (43%), Gaps = 12/126 (9%)
Frame = +3
Query: 333 GHPLTVYGKGGQTRAFLDIRDTVQCVELAIANP--ANPGEFRVFNQFTEQFKVTELAELV 390
G PL + G R F+ I+D ++ V L I NP AN F V N E V +LAE++
Sbjct: 780 GEPLKLVDGGESQRTFVYIKDAIEAVLLMIENPGRANGHIFNVGNPHNE-VTVRQLAEMM 956
Query: 391 TKAGEKLGLDVKTISVPNPRVELEEHY---YNCKNTKLVD-------LGLKPHFLSDSLI 440
+ K+ + + P V +E Y Y+ + ++ D LG P L+
Sbjct: 957 IQVYSKVSGE-QAPETPTLDVSSKEFYGEGYDDSDKRIPDMTIINRQLGWNPKTSLWDLL 1133
Query: 441 DSLLNF 446
+S L +
Sbjct: 1134ESTLTY 1151
>TC14436 homologue to UP|Q9AV98 (Q9AV98) UDP-D-glucuronate carboxy-lyase ,
partial (40%)
Length = 719
Score = 34.7 bits (78), Expect = 0.035
Identities = 21/59 (35%), Positives = 27/59 (45%), Gaps = 7/59 (11%)
Frame = +2
Query: 320 GTALNRFCVQAAVGHPLTVYGKGGQTRAFLDIRDTVQCV-------ELAIANPANPGEF 371
G ++ F QA PLTV G QTR+F + D V + + N NPGEF
Sbjct: 23 GRVVSNFIAQAIRNEPLTVQSPGTQTRSFCYVSDLVDGLIRLMDGSDTGPINLGNPGEF 199
>TC9292 homologue to UP|AAR07600 (AAR07600) Fiber dTDP-glucose
4-6-dehydratase (Fragment), partial (66%)
Length = 641
Score = 34.7 bits (78), Expect = 0.035
Identities = 30/93 (32%), Positives = 40/93 (42%), Gaps = 7/93 (7%)
Frame = +2
Query: 329 QAAVGHPLTVYGKGGQTRAFLDIRDTVQCV-------ELAIANPANPGEFRVFNQFTEQF 381
QA G PLTV G QTR+F + D V + + N NPGE F
Sbjct: 8 QALRGEPLTVQSPGTQTRSFCYVSDLVDGLIRLMEGSDTGPINLGNPGE----------F 157
Query: 382 KVTELAELVTKAGEKLGLDVKTISVPNPRVELE 414
+TELAE V + + NP+VE++
Sbjct: 158 TMTELAETVKE-------------LINPKVEIK 217
>AV768744
Length = 526
Score = 33.1 bits (74), Expect = 0.10
Identities = 16/67 (23%), Positives = 34/67 (49%), Gaps = 2/67 (2%)
Frame = -1
Query: 253 DTLPYPKQASSF--YHLSKVHDSHNIAFTCKAWGIRATDLNQGVVYGVRTDETAMHEELC 310
D +PY +Q+ + +H+ + ++C +WGI +T ++ + + +TA H+ +
Sbjct: 508 DRIPYLQQSMGIDDFGTDSLHNDSHETYSCNSWGIHSTSRSR---FHLEDKDTAFHKLIG 338
Query: 311 NRFDYDA 317
N D A
Sbjct: 337 NHMDCTA 317
>TC16761 similar to GB|AAL32703.1|17065098|AY062625 nucleotide sugar
epimerase-like protein {Arabidopsis thaliana;}, partial
(40%)
Length = 794
Score = 30.8 bits (68), Expect = 0.51
Identities = 32/137 (23%), Positives = 55/137 (39%), Gaps = 2/137 (1%)
Frame = +3
Query: 77 SSGDPFKPK--RVMVIGGDGYCGWATALHLSNKGYEVAIVDNLVRRLFDHQLGLDSLTPI 134
+S P +P V+V G G+ G +L L +G D LGLD+
Sbjct: 435 NSATPRRPNGMSVLVTGAVGFVGTHCSLALKKRG--------------DGVLGLDNFNTY 572
Query: 135 SSIQDRIQCWKSLTGKSIELYIGDICEFEFLSETFKSYEPDAVVHFGEQRSAPYSMIDRS 194
+ + L+ I + GDI + L++ F V+H Q Y+M +
Sbjct: 573 YDPSLKRARQELLSKHQIHVVDGDINDAPLLTKLFDVVPFTHVLHLAAQAGVRYAMQNPQ 752
Query: 195 RAVYTQQNNVVGTLNVL 211
+ ++N+ G +N+L
Sbjct: 753 SYI---RSNIAGFVNLL 794
>TC8032 similar to UP|Q9M0B6 (Q9M0B6) Nucleotide sugar epimerase-like
protein, partial (97%)
Length = 2230
Score = 30.4 bits (67), Expect = 0.66
Identities = 45/211 (21%), Positives = 76/211 (35%)
Frame = +3
Query: 87 VMVIGGDGYCGWATALHLSNKGYEVAIVDNLVRRLFDHQLGLDSLTPISSIQDRIQCWKS 146
V+V G G+ G +L L +G V +DN +D L T
Sbjct: 432 VLVTGAAGFVGSHVSLALKRRGDGVVGLDNF-NDYYDPSLKKARKT-------------L 569
Query: 147 LTGKSIELYIGDICEFEFLSETFKSYEPDAVVHFGEQRSAPYSMIDRSRAVYTQQNNVVG 206
LT + + GD+ + + L++ F V+H Q Y+M + V+ +N+
Sbjct: 570 LTTHGVFIVEGDVNDAKLLAKLFDVVAFTHVMHLAAQAGVRYAMENPHSYVH---SNIAA 740
Query: 207 TLNVLFAIKEYREDCHLVKLGTMGEYGTPNIDIEEGYITITHNGRTDTLPYPKQASSFYH 266
+ ++ A K +V + YG + + + RTD Q +S Y
Sbjct: 741 LVTLMEACKSANPQPAIVWASSSSVYGL------NEKVPFSESDRTD------QPASLYA 884
Query: 267 LSKVHDSHNIAFTCKAWGIRATDLNQGVVYG 297
+K +G+ T L VYG
Sbjct: 885 ATKKAGEEITHTYNHIYGLSITGLRFFTVYG 977
>AV406605
Length = 427
Score = 28.9 bits (63), Expect = 1.9
Identities = 15/55 (27%), Positives = 26/55 (47%), Gaps = 10/55 (18%)
Frame = +3
Query: 232 YGTPNIDIEEGYIT----------ITHNGRTDTLPYPKQASSFYHLSKVHDSHNI 276
+G+P++ E+ T T NG++ P+ Q SSF S+ H H++
Sbjct: 96 WGSPSLHSEQSKPTAWSRPSVGHPFTSNGKSHAFPFSSQNSSFVSSSQPHHHHHV 260
>TC13206 homologue to UP|Q9AV98 (Q9AV98) UDP-D-glucuronate carboxy-lyase ,
partial (51%)
Length = 650
Score = 28.1 bits (61), Expect = 3.3
Identities = 39/152 (25%), Positives = 59/152 (38%), Gaps = 2/152 (1%)
Frame = +2
Query: 86 RVMVIGGDGYCGWATALHL-SNKGYEVAIVDNLVRRLFDHQLGLDSLTPISSIQDRIQCW 144
R++V GG G+ G L N+ EV + DN + +D ++ W
Sbjct: 218 RILVTGGAGFIGSHLVDRLMENEKNEVIVADNY----------------FTGSKDNLKKW 349
Query: 145 KSLTGKSIELYIGDICEFEFLSETFKSYEPDAVVHFGEQRSAPYSMID-RSRAVYTQQNN 203
+ EL D+ E + E D + H + P S I + V T + N
Sbjct: 350 --IGHPRFELIRHDVTEPLLI-------EVDQIYHL----ACPASPIFYKYNPVKTIKTN 490
Query: 204 VVGTLNVLFAIKEYREDCHLVKLGTMGEYGTP 235
V+GTLN+L K R ++ T YG P
Sbjct: 491 VIGTLNMLGLAK--RVGARILLTSTSEVYGDP 580
>TC8795 similar to UP|GMD1_ARATH (Q9SNY3) GDP-mannose 4,6 dehydratase
(GDP-D-mannose dehydratase) (GMD) , partial (43%)
Length = 674
Score = 27.7 bits (60), Expect = 4.3
Identities = 21/55 (38%), Positives = 26/55 (47%), Gaps = 2/55 (3%)
Frame = +3
Query: 68 TGQEAPVQTSSGDPFKPKR--VMVIGGDGYCGWATALHLSNKGYEVAIVDNLVRR 120
+G E P T+ GD P R ++ G G G L NKGYE V L+RR
Sbjct: 147 SGSETPTSTN-GDASPPSRKVALITGITGQDGSYLTEFLLNKGYE---VHGLIRR 299
>TC15725 similar to UP|MDHG_ARATH (Q9ZP05) Malate dehydrogenase, glyoxysomal
precursor (mbNAD-MDH) , partial (39%)
Length = 586
Score = 27.3 bits (59), Expect = 5.6
Identities = 13/57 (22%), Positives = 28/57 (48%)
Frame = -2
Query: 30 SFSNTVVCDNSKTPFRQLFLREQKPRKSLAVVNASTISTGQEAPVQTSSGDPFKPKR 86
+FS+ ++ + + P + +PR++L S+++ T+ PFKP+R
Sbjct: 366 AFSSPILSYSFRGPS*YISSAPARPRRTLVAKKGSSVT*DSTKEHSTTPASPFKPRR 196
>TC16345 weakly similar to UP|Q8RX71 (Q8RX71) At3g51780/ORF3, partial (25%)
Length = 567
Score = 26.9 bits (58), Expect = 7.3
Identities = 14/29 (48%), Positives = 17/29 (58%)
Frame = -2
Query: 322 ALNRFCVQAAVGHPLTVYGKGGQTRAFLD 350
ALN+F VQA +P T+ G GG R D
Sbjct: 287 ALNQFYVQAVSLYPRTLIGVGGGARGERD 201
>TC14616
Length = 1353
Score = 26.9 bits (58), Expect = 7.3
Identities = 14/36 (38%), Positives = 17/36 (46%)
Frame = -3
Query: 238 DIEEGYITITHNGRTDTLPYPKQASSFYHLSKVHDS 273
D+ E Y R TLPY + S F H + V DS
Sbjct: 871 DLSENYFVY----RRQTLPYASKESFFLHSASVEDS 776
>AV428492
Length = 424
Score = 26.6 bits (57), Expect = 9.5
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = -1
Query: 6 SSSCSLTFSASNKPCLKPFHQCSTSFSNTV 35
S+S +TF+A+ P L + T+FSN V
Sbjct: 409 STSTEITFTATKAPSLSLVDRAKTTFSNLV 320
>TC8094 homologue to UP|EFTU_PEA (O24310) Elongation factor Tu, chloroplast
precursor (EF-Tu), partial (60%)
Length = 1092
Score = 26.6 bits (57), Expect = 9.5
Identities = 14/29 (48%), Positives = 18/29 (61%)
Frame = +1
Query: 6 SSSCSLTFSASNKPCLKPFHQCSTSFSNT 34
SSS S+T + S+K +P H STS S T
Sbjct: 94 SSSSSVTAAVSSKLATQPPHAFSTSISTT 180
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.135 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,147,899
Number of Sequences: 28460
Number of extensions: 106634
Number of successful extensions: 556
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 554
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 556
length of query: 477
length of database: 4,897,600
effective HSP length: 94
effective length of query: 383
effective length of database: 2,222,360
effective search space: 851163880
effective search space used: 851163880
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC125478.9