BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC125478.4 + phase: 0
(260 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9434 130 3e-31
TC9456 92 1e-19
BP074457 91 2e-19
TC9411 similar to UP|Q41102 (Q41102) Phaseolin G-box binding pro... 42 8e-05
TC18919 similar to GB|AAN73298.1|25141207|BT002301 At4g16430/dl4... 41 2e-04
CB827320 39 0.001
TC16664 similar to UP|BAD09830 (BAD09830) DNA-binding protein-li... 37 0.003
TC18477 36 0.008
BI419711 35 0.010
AV772495 30 0.32
TC18198 weakly similar to UP|Q9XGZ5 (Q9XGZ5) T1N24.20 protein, p... 29 0.71
TC16853 similar to UP|Q93ZP3 (Q93ZP3) At1g32359/F27G20.10, parti... 29 0.93
TC18633 similar to GB|AAB94051.1|2736075|AF016644 PAP-specific p... 28 1.2
TC9482 similar to UP|Q9FI73 (Q9FI73) Mitochondrial carrier prote... 28 2.1
BP036451 28 2.1
TC9509 weakly similar to UP|Q8RXD3 (Q8RXD3) ABI3-interacting pro... 28 2.1
CB828459 27 2.7
TC8026 27 2.7
AV780927 27 2.7
BP065117 27 4.6
>TC9434
Length = 599
Score = 130 bits (326), Expect = 3e-31
Identities = 67/86 (77%), Positives = 74/86 (85%), Gaps = 1/86 (1%)
Frame = +1
Query: 159 FVVSTSRLNDTELVIHISSYEAN-KIPLSEILMCLENNGLLLLNSSSSKTFGGRLFYNLH 217
FVVS S LNDTE IHISSYEA+ K PLSEIL+CLENNG LLLN+SS+KTFGGR+FYNLH
Sbjct: 4 FVVSNSWLNDTEAAIHISSYEAHHKTPLSEILLCLENNGYLLLNASSTKTFGGRIFYNLH 183
Query: 218 FQVDKTQRYECDDLIQKLSSIYEKQQ 243
FQV+KTQR E D L QKL SIYEK+Q
Sbjct: 184 FQVEKTQRLEADILTQKLLSIYEKKQ 261
>TC9456
Length = 513
Score = 91.7 bits (226), Expect = 1e-19
Identities = 50/78 (64%), Positives = 58/78 (74%), Gaps = 2/78 (2%)
Frame = +2
Query: 173 IHISSYEANKIPLSEILMCLENNGLLLLNSSSSKTFGGRLFYNLHFQVDKTQRYECDDLI 232
IHISS E PLSEIL+ LENNG LLN+SSS+TF GR+FYNLHFQV+K R E L
Sbjct: 8 IHISSCEVYMTPLSEILLYLENNGHFLLNASSSETFEGRVFYNLHFQVEKAHRIESCILT 187
Query: 233 QKLSSIYEKQQ--NNHLG 248
+KL SIYEK+Q +N LG
Sbjct: 188 EKLLSIYEKKQSISNQLG 241
>BP074457
Length = 527
Score = 91.3 bits (225), Expect = 2e-19
Identities = 43/57 (75%), Positives = 50/57 (87%)
Frame = -3
Query: 187 EILMCLENNGLLLLNSSSSKTFGGRLFYNLHFQVDKTQRYECDDLIQKLSSIYEKQQ 243
EIL+CLENNG LLN+SS++TFGGR+FYNLHFQV+KTQR E D L QKL SIYEK+Q
Sbjct: 525 EILLCLENNGYFLLNASSTETFGGRIFYNLHFQVEKTQRLESDILTQKLLSIYEKKQ 355
>TC9411 similar to UP|Q41102 (Q41102) Phaseolin G-box binding protein PG2
(Fragment), partial (30%)
Length = 705
Score = 42.4 bits (98), Expect = 8e-05
Identities = 30/107 (28%), Positives = 51/107 (47%), Gaps = 7/107 (6%)
Frame = +2
Query: 68 LNHNASERDRRKKINSLISSLRSLLPGEDQTKKMSIPVTISRVLKYIPDLQKQVQ----- 122
LNH +ER RR+K+N +LR+++P + K S+ + + YI +L+ ++Q
Sbjct: 185 LNHVEAERQRREKLNQRFYALRAVVPNVSKMDKASL---LGDAISYITELKTKLQSSESD 355
Query: 123 --GLTKKKEELLSRISHRQEYAVNKESQRKKIPNYNSAFVVSTSRLN 167
GL K+ + + + E S PN N +F S+S N
Sbjct: 356 KTGLQKQFDAMKKELEKTSE---QSSSPTPPPPNKNKSFSSSSSSSN 487
>TC18919 similar to GB|AAN73298.1|25141207|BT002301 At4g16430/dl4240w
{Arabidopsis thaliana;}, partial (29%)
Length = 604
Score = 40.8 bits (94), Expect = 2e-04
Identities = 21/62 (33%), Positives = 38/62 (60%)
Frame = +3
Query: 68 LNHNASERDRRKKINSLISSLRSLLPGEDQTKKMSIPVTISRVLKYIPDLQKQVQGLTKK 127
LNH +ER RR+K+N +LR+++P + K S+ + + +I DLQK+++ L +
Sbjct: 408 LNHVEAERQRREKLNQRFYALRAVVPNISKMDKASL---LGDAITHITDLQKKIKLLETE 578
Query: 128 KE 129
K+
Sbjct: 579 KD 584
>CB827320
Length = 522
Score = 38.5 bits (88), Expect = 0.001
Identities = 33/114 (28%), Positives = 55/114 (47%)
Frame = +1
Query: 22 EPESLISHKEKNYASLEYSLPYHQFSSPKEHVEIERPPSPKLMAKKLNHNASERDRRKKI 81
E + +E + L+ +L Y+ SSP + +K + SER+RRKK+
Sbjct: 118 ETNMFLQTQELDSWGLDEALSYYDSSSPDG-------AASSAASKNI---VSERNRRKKL 267
Query: 82 NSLISSLRSLLPGEDQTKKMSIPVTISRVLKYIPDLQKQVQGLTKKKEELLSRI 135
N + +LRS++P + K SI I ++YI L +Q + + + EL S I
Sbjct: 268 NERLFALRSVVPNISKMDKASI---IKDAIEYIEHLHEQEKIIQAEIMELESGI 420
>TC16664 similar to UP|BAD09830 (BAD09830) DNA-binding protein-like, partial
(22%)
Length = 1067
Score = 37.0 bits (84), Expect = 0.003
Identities = 16/50 (32%), Positives = 35/50 (70%)
Frame = +1
Query: 69 NHNASERDRRKKINSLISSLRSLLPGEDQTKKMSIPVTISRVLKYIPDLQ 118
NH+ +ER RR +IN+ + +LR+++PG ++ K S+ ++ V+ ++ +L+
Sbjct: 289 NHSEAERRRRARINAHLDTLRTVIPGANKMDKASL---LAEVITHLKELK 429
>TC18477
Length = 655
Score = 35.8 bits (81), Expect = 0.008
Identities = 22/59 (37%), Positives = 31/59 (52%)
Frame = +3
Query: 66 KKLNHNASERDRRKKINSLISSLRSLLPGEDQTKKMSIPVTISRVLKYIPDLQKQVQGL 124
K + H ER RR+ + S +SLRSLLP E K S+ I+ YI +Q ++ L
Sbjct: 282 KIMVHREIERKRRQDMASFYASLRSLLPLEFIKGKRSLSEHINEAANYIKHMQNNLKEL 458
>BI419711
Length = 539
Score = 35.4 bits (80), Expect = 0.010
Identities = 24/70 (34%), Positives = 37/70 (52%), Gaps = 4/70 (5%)
Frame = +1
Query: 28 SHKEKNYASLEYSLPYHQFSSPKEHVEIERPPSPKLMAK----KLNHNASERDRRKKINS 83
SH + + S P SS ++ R S K M + ++ H A ER+RRK++N
Sbjct: 274 SHTSPEACTFDQSSPTPFSSSRRKR---RRTKSAKNMEEIENQRMTHIAVERNRRKQMNE 444
Query: 84 LISSLRSLLP 93
+S+LRSL+P
Sbjct: 445 YLSALRSLMP 474
>AV772495
Length = 572
Score = 30.4 bits (67), Expect = 0.32
Identities = 16/46 (34%), Positives = 26/46 (55%)
Frame = -3
Query: 49 PKEHVEIERPPSPKLMAKKLNHNASERDRRKKINSLISSLRSLLPG 94
P HV + R + +H+ +ER RR+KIN+ + L+ L+PG
Sbjct: 468 PYVHVRVRRGQATD------SHSLAERARREKINARMKLLQELVPG 349
>TC18198 weakly similar to UP|Q9XGZ5 (Q9XGZ5) T1N24.20 protein, partial
(14%)
Length = 592
Score = 29.3 bits (64), Expect = 0.71
Identities = 22/77 (28%), Positives = 35/77 (44%), Gaps = 1/77 (1%)
Frame = -3
Query: 21 LEPESLISHKEKNYASLEYSLPYHQFSSPKEHVEIERPPSPKLMAKKLNHNASERDRRKK 80
+E + ++ NY S YS SSP +++ P PKL+ +LNH + +
Sbjct: 476 VEGQKKVNEAAVNYFSDIYS------SSPLQYLRECLAPIPKLVTDRLNHKLCAQVSTDE 315
Query: 81 INSLISSLRSL-LPGED 96
I + S+ L PG D
Sbjct: 314 IKEAVFSMGDLKAPGMD 264
>TC16853 similar to UP|Q93ZP3 (Q93ZP3) At1g32359/F27G20.10, partial (20%)
Length = 722
Score = 28.9 bits (63), Expect = 0.93
Identities = 13/40 (32%), Positives = 23/40 (57%)
Frame = -2
Query: 99 KKMSIPVTISRVLKYIPDLQKQVQGLTKKKEELLSRISHR 138
K+ ++P I ++YIP+L K + GL K + L + +R
Sbjct: 622 KR*TLPPNIKAQIRYIPELNKVLLGLEDKNHKTLPKDGNR 503
>TC18633 similar to GB|AAB94051.1|2736075|AF016644 PAP-specific phosphatase
{Arabidopsis thaliana;} , partial (45%)
Length = 882
Score = 28.5 bits (62), Expect = 1.2
Identities = 14/45 (31%), Positives = 26/45 (57%)
Frame = +1
Query: 87 SLRSLLPGEDQTKKMSIPVTISRVLKYIPDLQKQVQGLTKKKEEL 131
S RSL Q K +S+P+TI + ++ L++ +Q + +K+ L
Sbjct: 745 STRSLCGQTMQNKFLSLPLTIQHLPLFVNQLRRPIQAIPLRKDWL 879
>TC9482 similar to UP|Q9FI73 (Q9FI73) Mitochondrial carrier protein-like
(At5g48970), partial (42%)
Length = 529
Score = 27.7 bits (60), Expect = 2.1
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = +1
Query: 6 PPQFSYSNMGWLLEELEPESLI 27
PP FS+S++GW LE + S+I
Sbjct: 160 PPAFSFSSVGWRLEHVPNLSVI 225
>BP036451
Length = 511
Score = 27.7 bits (60), Expect = 2.1
Identities = 14/52 (26%), Positives = 29/52 (54%)
Frame = +2
Query: 73 SERDRRKKINSLISSLRSLLPGEDQTKKMSIPVTISRVLKYIPDLQKQVQGL 124
+E++RR+++N LRSL+P + + S+ + + YI +L + + L
Sbjct: 332 TEKERREQLNGKYKILRSLIPSPTKIDRASV---VGDAIDYIRELIRTLNEL 478
>TC9509 weakly similar to UP|Q8RXD3 (Q8RXD3) ABI3-interacting protein 2,
partial (42%)
Length = 642
Score = 27.7 bits (60), Expect = 2.1
Identities = 13/38 (34%), Positives = 23/38 (60%)
Frame = +2
Query: 56 ERPPSPKLMAKKLNHNASERDRRKKINSLISSLRSLLP 93
+R S L+ ++L + +++K S +SSL+SLLP
Sbjct: 86 KRMESEDLLKQELEELQKQLGKKQKFESAVSSLKSLLP 199
>CB828459
Length = 549
Score = 27.3 bits (59), Expect = 2.7
Identities = 25/86 (29%), Positives = 38/86 (44%), Gaps = 5/86 (5%)
Frame = +2
Query: 127 KKEELLSRISHRQEYA---VNKESQRKKIPNYNSAFVVSTSRLNDTELVIHISSYEANKI 183
KK + + H E+ +N S KIPN + + V S NDT+ H +S K
Sbjct: 200 KKRKFMDVSQHYVEHGKNNINDRSDSVKIPNLSGSLVWKNSSRNDTK-EKHGASKPKTKF 376
Query: 184 --PLSEILMCLENNGLLLLNSSSSKT 207
P S + + + L N+SS+ T
Sbjct: 377 GKPESVLGRVIPPKDISLSNASSTTT 454
>TC8026
Length = 667
Score = 27.3 bits (59), Expect = 2.7
Identities = 16/46 (34%), Positives = 25/46 (53%)
Frame = +3
Query: 42 PYHQFSSPKEHVEIERPPSPKLMAKKLNHNASERDRRKKINSLISS 87
P+H +P + RPPS + A NH +RD R++I+ + SS
Sbjct: 60 PHHPLQNP*RNFGF-RPPSAETAA---NHRLLQRDLRRRISRMWSS 185
>AV780927
Length = 496
Score = 27.3 bits (59), Expect = 2.7
Identities = 16/47 (34%), Positives = 26/47 (55%)
Frame = -2
Query: 42 PYHQFSSPKEHVEIERPPSPKLMAKKLNHNASERDRRKKINSLISSL 88
P+H +P + RPPS + A NH +RD R++I+ + SS+
Sbjct: 339 PHHPLPNP*RNFG-SRPPSAETAA---NHRLLQRDLRRRISRMWSSV 211
>BP065117
Length = 546
Score = 26.6 bits (57), Expect = 4.6
Identities = 14/48 (29%), Positives = 24/48 (49%)
Frame = +3
Query: 175 ISSYEANKIPLSEILMCLENNGLLLLNSSSSKTFGGRLFYNLHFQVDK 222
I +++ +P SE + ++L + K GG FY+LHF + K
Sbjct: 192 IENFQPVPLPKSEYGKFYMGDSYIILQTRQGK--GGTYFYDLHFWIGK 329
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.314 0.131 0.362
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,576,667
Number of Sequences: 28460
Number of extensions: 62873
Number of successful extensions: 285
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 283
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 283
length of query: 260
length of database: 4,897,600
effective HSP length: 88
effective length of query: 172
effective length of database: 2,393,120
effective search space: 411616640
effective search space used: 411616640
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 54 (25.4 bits)
Medicago: description of AC125478.4