BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC125477.15 - phase: 0
(738 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19422 weakly similar to UP|Q945L7 (Q945L7) AT4g35240/F23E12_20... 127 5e-30
BP064849 110 8e-25
TC16332 similar to UP|O64472 (O64472) At2g19090 protein, partial... 109 2e-24
CN825572 71 5e-13
TC13425 weakly similar to UP|Q7XJL5 (Q7XJL5) At2g17100 protein, ... 56 2e-08
BP056119 52 1e-07
BP053768 37 0.009
TC11310 similar to UP|BAD11331 (BAD11331) BRI1-KD interacting pr... 35 0.033
AW428678 34 0.096
AV411437 32 0.48
TC15452 similar to UP|SR40_YEAST (P32583) Suppressor protein SRP... 32 0.48
AU088795 31 0.81
TC11308 similar to UP|CENB_HUMAN (P07199) Major centromere autoa... 30 1.1
BP034655 30 1.1
TC14277 similar to UP|Q9SHY3 (Q9SHY3) F1E22.9 (AT1G65720/F1E22_1... 30 1.1
BP036655 30 1.4
TC9847 similar to UP|AAR14272 (AAR14272) Predicted protein, part... 30 1.4
TC14984 similar to GB|AAM62447.1|21553354|AY085214 glycine-rich ... 30 1.8
TC8052 similar to GB|AAP13431.1|30023796|BT006323 At5g02530 {Ara... 30 1.8
AV408441 29 2.4
>TC19422 weakly similar to UP|Q945L7 (Q945L7) AT4g35240/F23E12_200, partial
(39%)
Length = 624
Score = 127 bits (320), Expect = 5e-30
Identities = 74/174 (42%), Positives = 102/174 (58%)
Frame = +1
Query: 409 DPSEEQCVFSVSHQSTLDRLYAWEKKLYQEVKSGTRVRLAYEKKCLQLRNHDIKGEEPSS 468
DP S STL +LY WEKKLY EVK+ ++R+ +++KC +L+ D +G + +
Sbjct: 124 DPDVGLSTTSRHLSSTLQKLYLWEKKLYHEVKAEEKMRVDHDRKCRKLKRLDERGADFNK 303
Query: 469 VDKTRAAIRDLHTQITVSIHSVEAISRRIETLRDEELHSQLLELLQGLAKMWKVMAECHQ 528
VD TR +IR L T+I ++I V+ IS I +RD EL L EL+QGL +MWK M ECH
Sbjct: 304 VDTTRTSIRSLSTKIKMAIQVVDKISMTISNIRDGELWPLLKELIQGLTRMWKSMLECHH 483
Query: 529 TQKQTLDEAKILIAGIDARKQSSMSITDPYRLARSASNLETELRNWRNTFESWI 582
+Q + + EA+IL I +RK S D + LA LE +L NW F WI
Sbjct: 484 SQCEAIREARIL-GSIGSRKNSG----DSHLLATKL--LEQDLINWTFQFSGWI 624
>BP064849
Length = 498
Score = 110 bits (275), Expect = 8e-25
Identities = 55/90 (61%), Positives = 68/90 (75%), Gaps = 2/90 (2%)
Frame = +2
Query: 1 MGCSQSKLDDEEAVKICKDRKRFIKEAVEHRTQFANGHIAYIQSLKRVSAALLDYFEENE 60
MGCS SKL+DEE+V++CKDRK+FIK+AVE RT+FA GH+AYI+SL RVSAAL DY E +E
Sbjct: 224 MGCSHSKLEDEESVQLCKDRKKFIKQAVEQRTRFATGHMAYIESLSRVSAALRDYIEGDE 403
Query: 61 ALEFSFDSFVTPP--AKNKASPAVISISKH 88
EFS DS + PP K P I IS++
Sbjct: 404 PREFSLDSVMNPPFTPVKKTGPGFIPISQN 493
>TC16332 similar to UP|O64472 (O64472) At2g19090 protein, partial (4%)
Length = 543
Score = 109 bits (272), Expect = 2e-24
Identities = 59/98 (60%), Positives = 70/98 (71%), Gaps = 6/98 (6%)
Frame = +1
Query: 642 LDSIDFFAAELGSFYAQQLREDSAQNATVGSNVNMEMVEVAKVGEEVM------EVAVKV 695
LD +DFFAA +GS YA QLR+DS T G MEMVE +V EEVM EVA+KV
Sbjct: 1 LDGLDFFAAGMGSLYAHQLRQDSRPRPTHGG---MEMVEFGQVEEEVMAPEKLAEVAIKV 171
Query: 696 LCGGMSGAMSSMSEFAIDSAKGYNELVKQWENAKLQQT 733
LC GMS AMSS++EFA DSA+GY+E++KQWEN K Q T
Sbjct: 172 LCAGMSVAMSSLAEFAFDSAEGYSEVIKQWENVKSQNT 285
>CN825572
Length = 619
Score = 71.2 bits (173), Expect = 5e-13
Identities = 50/192 (26%), Positives = 91/192 (47%)
Frame = +2
Query: 471 KTRAAIRDLHTQITVSIHSVEAISRRIETLRDEELHSQLLELLQGLAKMWKVMAECHQTQ 530
KT+ +I L + ITV+ +V S I LRD +L QL+EL G+ MW+ M + H+ Q
Sbjct: 5 KTKTSINRLQSLITVTSQAVSTTSTAIIGLRDSDLVPQLVELCHGIMYMWRSMHQYHEIQ 184
Query: 531 KQTLDEAKILIAGIDARKQSSMSITDPYRLARSASNLETELRNWRNTFESWITSQKSYIH 590
+ + + G+ R S ++ +R ++ +LE+ + W ++F I Q+ ++
Sbjct: 185 SHIVQQ----VRGLVNRTSRGHSTSELHR--QATRDLESAVSAWHSSFCRLIKFQRDFVL 346
Query: 591 ALTGWLLRCMRCEPDASKLICSPRRSSSTHPLFGLCIQWSRRLDALEETAVLDSIDFFAA 650
+L GW + D I + R +S + F +W LD + +T ++I F
Sbjct: 347 SLHGW-FKLSLVPVDTDNNIHNSREASDAYMFFD---EWKLALDRVPDTVASEAIKSFIN 514
Query: 651 ELGSFYAQQLRE 662
+ ++Q E
Sbjct: 515 VIHVISSKQAEE 550
>TC13425 weakly similar to UP|Q7XJL5 (Q7XJL5) At2g17100 protein, partial
(6%)
Length = 538
Score = 56.2 bits (134), Expect = 2e-08
Identities = 48/181 (26%), Positives = 75/181 (40%), Gaps = 1/181 (0%)
Frame = +1
Query: 1 MGCSQSKLDDEEAVKICKDRKRFIKEAVEHRTQFANGHIAYIQSLKRVSAALLDYFEE-N 59
MGC+ S+LD A +C+DR +F EA+ A+ H+A++ SL+ + AL+ +F+
Sbjct: 64 MGCNTSRLDHLPAAALCRDRCKFADEALRQSYALADAHVAHMDSLRTLGPALVCFFDHFQ 243
Query: 60 EALEFSFDSFVTPPAKNKASPAVISISKHSSPTTIEFGPNTTTLKVNYLRPSGNPPISVE 119
E S S PP P ++ HS P + P + ++ + N P +
Sbjct: 244 ETKTVSILSSPPPP------PLPVAAPPHSPPPCSDEFPLHSESDDDH--DADNEPPRYD 399
Query: 120 ERPPSPEMVRVEMYSPMYQYGMDGFFGMQSQPMNASIFAYSPNNRPVIPPASPQSSQWDS 179
+ + SP Y GF+G +S P P P SS WD
Sbjct: 400 DYLNHRILSPATPLSPPYS---GGFWGSKSAP----------------SPPPPTSSAWDL 522
Query: 180 F 180
F
Sbjct: 523 F 525
>BP056119
Length = 427
Score = 51.6 bits (122), Expect(2) = 1e-07
Identities = 33/63 (52%), Positives = 37/63 (58%), Gaps = 6/63 (9%)
Frame = -2
Query: 652 LGSFYAQQLREDSAQNATVGSNVNMEMVEVAKVGEEVM------EVAVKVLCGGMSGAMS 705
+GS YA LR D T G MEMVE V EEVM EVA+KVLC GMS AMS
Sbjct: 423 MGSLYAHPLRPDFRHRPTHGG---MEMVEFGPVEEEVMAPGKLAEVAIKVLCAGMSVAMS 253
Query: 706 SMS 708
S++
Sbjct: 252 SLA 244
Score = 21.2 bits (43), Expect(2) = 1e-07
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = -3
Query: 709 EFAIDSAKGY 718
EFA DSA+GY
Sbjct: 242 EFAFDSAEGY 213
>BP053768
Length = 561
Score = 37.4 bits (85), Expect = 0.009
Identities = 17/25 (68%), Positives = 19/25 (76%)
Frame = +3
Query: 423 STLDRLYAWEKKLYQEVKSGTRVRL 447
STLDRL AWEKKLY EVK+ V +
Sbjct: 114 STLDRLLAWEKKLYLEVKNHVHVEI 188
Score = 36.6 bits (83), Expect = 0.015
Identities = 18/77 (23%), Positives = 41/77 (52%)
Frame = +1
Query: 519 MWKVMAECHQTQKQTLDEAKILIAGIDARKQSSMSITDPYRLARSASNLETELRNWRNTF 578
MW+ M + H+TQ + + + G+ R + S ++ ++ ++ +LE+ + +W ++F
Sbjct: 178 MWRSMHQHHETQSSIVKQ----VRGLVNRSSKNHSTSETHK--QATRDLESAVSSWHSSF 339
Query: 579 ESWITSQKSYIHALTGW 595
I Q+ +I +L W
Sbjct: 340 NCLIKFQRDFILSLHSW 390
>TC11310 similar to UP|BAD11331 (BAD11331) BRI1-KD interacting protein 102
(Fragment), partial (13%)
Length = 667
Score = 35.4 bits (80), Expect = 0.033
Identities = 23/83 (27%), Positives = 42/83 (49%)
Frame = +1
Query: 196 SSLEQIVMDDENRGLRKVREEEGIPDLEQEEMDDEQEGCVVKRNVAEERTKIDVNSSKEE 255
+S ++ + E + KV+ E+G PDL+Q E ++ K++ E ++++ K
Sbjct: 133 ASSDEEKAEKEKKKKHKVKVEDGSPDLDQSEKKKKK-----KKDQDAEDNAAEISNGK-- 291
Query: 256 VMVEDVDKHKEEEKEKGTDAETE 278
ED K+ +K+K DAE E
Sbjct: 292 ---EDRKSEKKHKKKKNQDAEEE 351
Score = 27.7 bits (60), Expect = 6.9
Identities = 19/82 (23%), Positives = 38/82 (46%)
Frame = +1
Query: 206 ENRGLRKVREEEGIPDLEQEEMDDEQEGCVVKRNVAEERTKIDVNSSKEEVMVEDVDKHK 265
E G +K ++++ D E E+ E K + + K++ S D+D+ +
Sbjct: 67 EVEGEKKEKKKKKKKDQENGEVASSDEEKAEKEKKKKHKVKVEDGSP-------DLDQSE 225
Query: 266 EEEKEKGTDAETETAQEISDSK 287
+++K+K + A EIS+ K
Sbjct: 226 KKKKKKKDQDAEDNAAEISNGK 291
>AW428678
Length = 394
Score = 33.9 bits (76), Expect = 0.096
Identities = 17/53 (32%), Positives = 26/53 (48%)
Frame = +2
Query: 72 PPAKNKASPAVISISKHSSPTTIEFGPNTTTLKVNYLRPSGNPPISVEERPPS 124
PP + SP S S ++P T N T ++++ P G+PP+ PPS
Sbjct: 125 PPPYHYVSPPPPSPSSSTTPKTTTNKSNPNTARLSHAAP*GSPPLLTTTSPPS 283
>AV411437
Length = 417
Score = 31.6 bits (70), Expect = 0.48
Identities = 36/138 (26%), Positives = 54/138 (39%), Gaps = 22/138 (15%)
Frame = +2
Query: 251 SSKEEVMVEDVDKHKEEEKEKGTDAE-----------------TETAQEISDSKINGGEC 293
SS +E + E+ ++ +EEE+E G D E TE E+ D+++ G
Sbjct: 32 SSAQETIAEEEEEEEEEEEEDGVDGELEQFDDDETDSEDDELETEGGGELEDAQVGG--- 202
Query: 294 FQVSKSQSSGHMESSHKEMAIDTEEAKEKTPGFTVYVNRRPENMAEVI-KDLESQFT--- 349
S SS +E E K + P TV + + A + K L+SQ
Sbjct: 203 -----STSSPLVEKR-------KERLKLEVPSLTVKEKKELASFAHSLGKKLKSQLVGKS 346
Query: 350 -VVCNAANDVSALLEAKK 366
V N A LEA +
Sbjct: 347 GVTSNLATSFIETLEANE 400
>TC15452 similar to UP|SR40_YEAST (P32583) Suppressor protein SRP40, partial
(4%)
Length = 589
Score = 31.6 bits (70), Expect = 0.48
Identities = 19/64 (29%), Positives = 33/64 (50%)
Frame = +2
Query: 248 DVNSSKEEVMVEDVDKHKEEEKEKGTDAETETAQEISDSKINGGECFQVSKSQSSGHMES 307
++ S E ++++++K + EEKE + E ++ SK+ G + S S SS ES
Sbjct: 371 NLTSDSGEGLLKELEKLRAEEKELKRKRKDEKKAKLKASKMKTGPDCESSSSSSSESSES 550
Query: 308 SHKE 311
S E
Sbjct: 551 SASE 562
>AU088795
Length = 482
Score = 30.8 bits (68), Expect = 0.81
Identities = 15/35 (42%), Positives = 19/35 (53%)
Frame = +2
Query: 138 QYGMDGFFGMQSQPMNASIFAYSPNNRPVIPPASP 172
QYG+ M S P S A SP++ P PP+SP
Sbjct: 284 QYGVCAINAMASYPTKQSXTAPSPSSTPPSPPSSP 388
>TC11308 similar to UP|CENB_HUMAN (P07199) Major centromere autoantigen B
(Centromere protein B) (CENP-B), partial (4%)
Length = 756
Score = 30.4 bits (67), Expect = 1.1
Identities = 16/43 (37%), Positives = 25/43 (57%)
Frame = -2
Query: 191 GYPNGSSLEQIVMDDENRGLRKVREEEGIPDLEQEEMDDEQEG 233
G +GS +I +++N G +V EEG D E+E+ +DE G
Sbjct: 350 GDEDGSRFHEI--ENDNEGEEEVEAEEGEEDEEEEDDEDEDPG 228
>BP034655
Length = 517
Score = 30.4 bits (67), Expect = 1.1
Identities = 32/140 (22%), Positives = 56/140 (39%), Gaps = 14/140 (10%)
Frame = +1
Query: 168 PPASPQSSQWDSFWNPFTSLDYFGYPNGSSLEQIVMDDENRGLRKVREEEGIPDLEQEEM 227
P ASP + + FG + E+ ++E + EEE + E+++
Sbjct: 58 PMASPSEFHRNEIAAAHLTAADFGLSDDDEEEEEEEEEEEEEEEEEEEEEEEEEDEEDDE 237
Query: 228 DDEQEGCV-------VKRNVAEERTKIDV------NSSKEEVMVEDVDKHKE-EEKEKGT 273
+DE E + V NV + T V +S ++ ED +K + EE E T
Sbjct: 238 EDEDEDYIPVAPSHRVSPNVHQNPTSDSVEVILIPDSEQQNYSEEDGNKRRRIEEGEAPT 417
Query: 274 DAETETAQEISDSKINGGEC 293
+ E++ + I+G C
Sbjct: 418 SSLGSQGNELTGTDIDGLVC 477
>TC14277 similar to UP|Q9SHY3 (Q9SHY3) F1E22.9 (AT1G65720/F1E22_13), partial
(33%)
Length = 941
Score = 30.4 bits (67), Expect = 1.1
Identities = 18/59 (30%), Positives = 27/59 (45%), Gaps = 7/59 (11%)
Frame = +3
Query: 73 PAKNKASPAVISISKHSSPTTIEFGPNTTTLKVN-------YLRPSGNPPISVEERPPS 124
P ++ P+ +S S + T+ P TTT K N + P G+PP+ PPS
Sbjct: 207 PPSPRSDPSPLSTSTTNPTTSSSTTPKTTTNKSNPNTARLSHAAP*GSPPLLTTTSPPS 383
>BP036655
Length = 556
Score = 30.0 bits (66), Expect = 1.4
Identities = 24/93 (25%), Positives = 46/93 (48%), Gaps = 1/93 (1%)
Frame = -3
Query: 215 EEEGIPDL-EQEEMDDEQEGCVVKRNVAEERTKIDVNSSKEEVMVEDVDKHKEEEKEKGT 273
EEEG+ D + +E+ DE+E V + + ++ + + EE +++ KEEE+E+
Sbjct: 410 EEEGLSDDGDDDEVVDEEEEVVEEWSDDDDINDDESDDEVEEESETEIETEKEEEEEEEC 231
Query: 274 DAETETAQEISDSKINGGECFQVSKSQSSGHME 306
E E+ K N + S+ + SG+ +
Sbjct: 230 GVE-NVEDEMGVKKFN-----RPSRCKESGNRD 150
>TC9847 similar to UP|AAR14272 (AAR14272) Predicted protein, partial (73%)
Length = 952
Score = 30.0 bits (66), Expect = 1.4
Identities = 33/135 (24%), Positives = 54/135 (39%), Gaps = 12/135 (8%)
Frame = -3
Query: 582 ITSQKSYIHALTGWLLRCMRCEPDASKLICSPRRSSSTHPLFGLCIQWSRRLDALEETAV 641
++++ + + T W+ +C R +S SP S S L I W L L T+
Sbjct: 659 LSTRARLLRSSTSWICKCSRHRESSSSCSFSPSISLSF-----LFISWMAILRMLSTTST 495
Query: 642 LDSIDFF--AAELGSFYAQQLREDSAQNAT--------VGSNVNMEMVEVAKVGEEVMEV 691
L S FF + G F A + S +A +GSN + + ++ V
Sbjct: 494 LTSFRFFPMSHPFG*FAASKSGGGSRDSAEGRRPLMFFIGSNGSNKAFRRSESSSSVTAD 315
Query: 692 A--VKVLCGGMSGAM 704
A + L GG+S +
Sbjct: 314 ASCISTLGGGVSSCL 270
>TC14984 similar to GB|AAM62447.1|21553354|AY085214 glycine-rich RNA binding
protein 7 {Arabidopsis thaliana;} , partial (19%)
Length = 483
Score = 29.6 bits (65), Expect = 1.8
Identities = 32/113 (28%), Positives = 48/113 (42%), Gaps = 1/113 (0%)
Frame = +2
Query: 72 PPAKNKASPAVISISKHSSPTTIEFGPNTTTLKVNYLRPSGNPPISV-EERPPSPEMVRV 130
PP + +SPA + S S+ T +TT S PP ++ + P+P R
Sbjct: 170 PPPEMPSSPATLKSSPSSATATAAPSTKSTT--------SPPPPSTL*KSSTPAPTPPRA 325
Query: 131 EMYSPMYQYGMDGFFGMQSQPMNASIFAYSPNNRPVIPPASPQSSQWDSFWNP 183
+ SP + P ++ A S N PPA+ QSS W++ WNP
Sbjct: 326 AVPSPK----STSSAAPPTAPTSSGTTAPSKN-----PPATSQSS-WNT-WNP 451
>TC8052 similar to GB|AAP13431.1|30023796|BT006323 At5g02530 {Arabidopsis
thaliana;}, partial (55%)
Length = 1389
Score = 29.6 bits (65), Expect = 1.8
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = +3
Query: 213 VREEEGIPDLEQEEMDDEQEGCVVKR 238
V E+EG+ E+E+ D+ EG VVKR
Sbjct: 750 VMEDEGVAIAEEEDEDEGMEGVVVKR 827
>AV408441
Length = 314
Score = 29.3 bits (64), Expect = 2.4
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = +2
Query: 363 EAKKAQYLSPSNELSASKLLNPVALFRSSPSKIITNFSKTR 403
E ++ Y +P N +A KLL+ S P K+I + K R
Sbjct: 101 EGRRVSYGTPENATAARKLLDRQMSINSVPKKVIAHLLKPR 223
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.313 0.128 0.361
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,598,809
Number of Sequences: 28460
Number of extensions: 158344
Number of successful extensions: 836
Number of sequences better than 10.0: 55
Number of HSP's better than 10.0 without gapping: 818
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 826
length of query: 738
length of database: 4,897,600
effective HSP length: 97
effective length of query: 641
effective length of database: 2,136,980
effective search space: 1369804180
effective search space used: 1369804180
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 59 (27.3 bits)
Medicago: description of AC125477.15