BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC125477.10 - phase: 0 /pseudo
(355 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17786 similar to PIR|T46177|T46177 villin 3 homolog T8H10.10 -... 203 3e-73
BP055885 177 1e-48
BP065117 136 6e-33
BP069941 37 0.007
TC18857 homologue to UP|Q9ZS21 (Q9ZS21) Glyoxalase I , complete 27 4.0
TC10784 weakly similar to UP|BAD01556 (BAD01556) ERF-like protei... 27 4.0
TC10840 homologue to UP|NDK1_SOYBN (Q39839) Nucleoside diphospha... 27 6.8
AW720445 27 6.8
TC16267 similar to GB|AAP68282.1|31711852|BT008843 At1g73060 {Ar... 26 8.9
>TC17786 similar to PIR|T46177|T46177 villin 3 homolog T8H10.10 -
Arabidopsis thaliana (fragment) {Arabidopsis thaliana;},
partial (42%)
Length = 1228
Score = 203 bits (517), Expect(3) = 3e-73
Identities = 96/127 (75%), Positives = 109/127 (85%)
Frame = +3
Query: 1 TTASKSGALRHDIHYWLGKDTSQDEAGAAAIKTVELDAVLGGRAVQYREVQGHETQKFLS 60
TT K G+ DIH+W+GKDTSQDEAG AAIKTVELDA LGGRAVQ+RE+QGHE+ KFLS
Sbjct: 459 TTQGKGGSYLFDIHFWIGKDTSQDEAGTAAIKTVELDASLGGRAVQHREIQGHESDKFLS 638
Query: 61 YFKPCIIPQEGGAASGFKHVEAEEHKTRLFVCKGKHVVYVKEVPFARSSLNHDDIFILDT 120
YFKPCIIP EGG ASGFK E EE +TRL+VCKGK VV +K+VPFARSSLNHDD+FILDT
Sbjct: 639 YFKPCIIPLEGGVASGFKTPEEEEFETRLYVCKGKRVVRIKQVPFARSSLNHDDVFILDT 818
Query: 121 ESKIFQF 127
+ KI+QF
Sbjct: 819 QDKIYQF 839
Score = 84.7 bits (208), Expect(3) = 3e-73
Identities = 39/66 (59%), Positives = 51/66 (77%)
Frame = +1
Query: 129 GSNSSIQERAKALEVVQYIKDTYHDGKCEVASIEDGRLMADSESGEFWGLFGGFAPLPRK 188
G+NS+IQERAKALEV+Q +K+ YH+GKC+VA ++DG+L +S+SGEFW LFG F P K
Sbjct: 844 GANSNIQERAKALEVIQLLKEKYHEGKCDVAIVDDGKLDTESDSGEFWVLFGWFCPHG*K 1023
Query: 189 TVSDDD 194
DD
Sbjct: 1024GDRGDD 1041
Score = 25.0 bits (53), Expect(3) = 3e-73
Identities = 12/17 (70%), Positives = 13/17 (75%), Gaps = 1/17 (5%)
Frame = +3
Query: 233 LDCGLEVFVWIGR-NTS 248
LDCG EV+VW R NTS
Sbjct: 1161 LDCGAEVYVWGWRGNTS 1211
>BP055885
Length = 464
Score = 177 bits (449), Expect(2) = 1e-48
Identities = 81/144 (56%), Positives = 115/144 (79%)
Frame = -3
Query: 89 LFVCKGKHVVYVKEVPFARSSLNHDDIFILDTESKIFQFNGSNSSIQERAKALEVVQYIK 148
L+VCKGK V +KEVPFARSSLNHDD+FIL+T++KI+QFNG+NS+IQERAKALEV+Q++K
Sbjct: 459 LYVCKGKRAVRLKEVPFARSSLNHDDVFILNTQNKIYQFNGANSNIQERAKALEVIQFLK 280
Query: 149 DTYHDGKCEVASIEDGRLMADSESGEFWGLFGGFAPLPRKTVSDDDKTIDSHPPKLLCVE 208
+ YH+GKC+VA ++DG+L +S+SGEFW LFGGFAP+ +K +S+DD ++ P +L +
Sbjct: 279 EKYHEGKCDVAIVDDGKLDTESDSGEFWVLFGGFAPIGKKVISEDDNIPEAIPAQLYSIV 100
Query: 209 KGKAEPFETDSLTKELLDTNKCYI 232
G+ + E + L+K LL+ +I
Sbjct: 99 DGEVKSIEGE-LSKSLLENTMLFI 31
Score = 32.3 bits (72), Expect(2) = 1e-48
Identities = 11/13 (84%), Positives = 12/13 (91%)
Frame = -1
Query: 230 CYILDCGLEVFVW 242
CY+LDCG EVFVW
Sbjct: 41 CYLLDCGAEVFVW 3
>BP065117
Length = 546
Score = 136 bits (342), Expect = 6e-33
Identities = 63/91 (69%), Positives = 73/91 (79%)
Frame = +3
Query: 1 TTASKSGALRHDIHYWLGKDTSQDEAGAAAIKTVELDAVLGGRAVQYREVQGHETQKFLS 60
T K G +D+H+W+GKDTSQDEAG AAIK VELDA LGGRAVQ+RE+QGHE+ KFLS
Sbjct: 273 TRQGKGGTYFYDLHFWIGKDTSQDEAGTAAIKAVELDAALGGRAVQHREIQGHESDKFLS 452
Query: 61 YFKPCIIPQEGGAASGFKHVEAEEHKTRLFV 91
YFKPCIIP EGG ASGF+ E EE +T L+V
Sbjct: 453 YFKPCIIPLEGGVASGFRKPEEEEFETHLYV 545
>BP069941
Length = 304
Score = 36.6 bits (83), Expect = 0.007
Identities = 25/87 (28%), Positives = 40/87 (45%), Gaps = 5/87 (5%)
Frame = +1
Query: 211 KAEPFETDSLTKELLDTNKCYILDCGLEVFVWIGRNTSLDERKSASGSTDELVSSTN--- 267
K + E + +++ L T ILD EVFVWIG++ E+++A + +
Sbjct: 13 KLQEEEVYNFSQDDLLTEDILILDTHAEVFVWIGQSVDTKEKQNAFEIAQKYIDKATSLE 192
Query: 268 --RPKSQIIRVMEGFETVMFRSKFDSW 292
P + +V EG E F + F SW
Sbjct: 193 GLSPHVPLYKVTEGNEPCFFTTYF-SW 270
>TC18857 homologue to UP|Q9ZS21 (Q9ZS21) Glyoxalase I , complete
Length = 877
Score = 27.3 bits (59), Expect = 4.0
Identities = 11/16 (68%), Positives = 11/16 (68%)
Frame = +2
Query: 228 NKCYILDCGLEVFVWI 243
NKCYI CGL VWI
Sbjct: 713 NKCYISCCGLVEHVWI 760
>TC10784 weakly similar to UP|BAD01556 (BAD01556) ERF-like protein, partial
(11%)
Length = 441
Score = 27.3 bits (59), Expect = 4.0
Identities = 13/38 (34%), Positives = 19/38 (49%)
Frame = -3
Query: 167 MADSESGEFWGLFGGFAPLPRKTVSDDDKTIDSHPPKL 204
++ SES W +F P P + S DD D +P +L
Sbjct: 139 ISSSESFSTWFVFLSSFPTPSSSASTDDPEADPNPAQL 26
>TC10840 homologue to UP|NDK1_SOYBN (Q39839) Nucleoside diphosphate kinase I
(NDK I) (NDP kinase I) , complete
Length = 540
Score = 26.6 bits (57), Expect = 6.8
Identities = 19/70 (27%), Positives = 29/70 (41%)
Frame = +2
Query: 203 KLLCVEKGKAEPFETDSLTKELLDTNKCYILDCGLEVFVWIGRNTSLDERKSASGSTDEL 262
KLL VE AE D K + YI+ + +W G+N RK +
Sbjct: 152 KLLTVESSFAEKHYADLSAKPFFNGLVEYIISGPVVAMIWEGKNVVTTGRK--------I 307
Query: 263 VSSTNRPKSQ 272
+ +TN +S+
Sbjct: 308 IGATNPAQSE 337
>AW720445
Length = 500
Score = 26.6 bits (57), Expect = 6.8
Identities = 12/28 (42%), Positives = 17/28 (59%), Gaps = 4/28 (14%)
Frame = -2
Query: 176 WGLFGG----FAPLPRKTVSDDDKTIDS 199
WGL G F LPR+ ++D+DK + S
Sbjct: 418 WGLNGRQGMQFKELPRRVITDEDKALGS 335
>TC16267 similar to GB|AAP68282.1|31711852|BT008843 At1g73060 {Arabidopsis
thaliana;}, partial (21%)
Length = 418
Score = 26.2 bits (56), Expect = 8.9
Identities = 16/46 (34%), Positives = 22/46 (47%), Gaps = 10/46 (21%)
Frame = -2
Query: 61 YFKPCIIPQEGGAASG-FKHVEAEEHKT---------RLFVCKGKH 96
Y K C + G + G H+E +E T R++VCKGKH
Sbjct: 342 YMKICYWAKAGNQSRGEVFHLEEQERGTWQHQ*RELHRIWVCKGKH 205
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.135 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,773,456
Number of Sequences: 28460
Number of extensions: 73152
Number of successful extensions: 336
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 333
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 336
length of query: 355
length of database: 4,897,600
effective HSP length: 91
effective length of query: 264
effective length of database: 2,307,740
effective search space: 609243360
effective search space used: 609243360
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC125477.10