BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124972.5 - phase: 0
(222 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC15656 similar to GB|AAP68317.1|31711922|BT008878 At5g64430 {Ar... 113 2e-26
TC8486 similar to GB|AAP68317.1|31711922|BT008878 At5g64430 {Ara... 104 1e-23
TC16174 similar to UP|Q940N7 (Q940N7) AT4g05150/C17L7_70, partia... 73 4e-14
AW719831 57 2e-09
TC10463 similar to UP|Q9LRV7 (Q9LRV7) Gb|AAF04880.1, partial (19%) 37 0.003
BU494127 34 0.018
TC18336 similar to UP|Q84KA9 (Q84KA9) RING/C3HC4/PHD zinc finger... 29 0.56
BP053180 29 0.56
TC18688 weakly similar to UP|Q9LP77 (Q9LP77) T1N15.9, partial (13%) 29 0.74
TC18261 similar to UP|WSC2_YEAST (P53832) Cell wall integrity an... 29 0.74
AV409705 28 1.3
TC15921 weakly similar to GB|AAB64701.1|603415|SCE9163 Yer174cp ... 28 1.3
AU251432 28 1.6
CN824958 27 2.1
BP053199 27 2.1
TC14475 27 2.1
TC19458 weakly similar to UP|Q9STE8 (Q9STE8) Chloroplast import-... 25 2.5
AV424458 27 2.8
TC19039 similar to UP|Q8L7W5 (Q8L7W5) AT4g12110/F16J13_180, part... 27 2.8
AU089438 27 2.8
>TC15656 similar to GB|AAP68317.1|31711922|BT008878 At5g64430 {Arabidopsis
thaliana;}, partial (23%)
Length = 846
Score = 113 bits (283), Expect = 2e-26
Identities = 71/161 (44%), Positives = 97/161 (60%), Gaps = 11/161 (6%)
Frame = +3
Query: 3 RVKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFMFS---- 58
+VK M +YGGKIQPR HD+ + SY+GGD KI+ VDRNI F L++KLS +
Sbjct: 168 KVKFMCSYGGKIQPRT---HDN-QLSYVGGDTKILAVDRNIKFPALLSKLSALCNNCDNP 335
Query: 59 -DVCFKYQLLGEDFDALIPIYNEEDLNHIMFEYDRMCHFSRKPAWLRVFLF----PVPIN 113
D+ FKYQL GED DALI + N++DL+H+M EYDR+ S +P+ +R+FLF P+P
Sbjct: 336 ADLTFKYQLPGEDLDALISVTNDDDLDHMMHEYDRLYRASARPSRMRLFLFLPENPLPSA 515
Query: 114 NNKASF-DSLASVKSLNFVRVSPFEE-YSSPPTTPPPPFMA 152
A D VK N + ++ ++PP PPP A
Sbjct: 516 LAPAPVSDQPDPVKPNNVDFLFGLDKPVAAPPPLQPPPLAA 638
>TC8486 similar to GB|AAP68317.1|31711922|BT008878 At5g64430 {Arabidopsis
thaliana;}, partial (25%)
Length = 856
Score = 104 bits (259), Expect = 1e-23
Identities = 65/159 (40%), Positives = 93/159 (57%), Gaps = 7/159 (4%)
Frame = +1
Query: 3 RVKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFMFS---D 59
+ K + +YGGKIQPR HD+ + SY+GGD KI+ VDRN F ++KL+ + +
Sbjct: 151 KAKFLCSYGGKIQPRT---HDN-QLSYVGGDTKILAVDRNTKFPTFLSKLAALCDAAPQE 318
Query: 60 VCFKYQLLGEDFDALIPIYNEEDLNHIMFEYDRMCHFSRKPAWLRVFLF----PVPINNN 115
+ FKYQL GED DALI + N++DL H+M EYDR+ + KP +R+FLF P P++
Sbjct: 319 LTFKYQLPGEDLDALISVTNDDDLEHLMHEYDRLYRPASKPVRMRLFLFSAPNPGPLSQQ 498
Query: 116 KASFDSLASVKSLNFVRVSPFEEYSSPPTTPPPPFMATK 154
D L +++F + E+ PP PP F A K
Sbjct: 499 P---DPLKPQPNVDF--LFGLEKGVVPP-PQPPSFAAVK 597
>TC16174 similar to UP|Q940N7 (Q940N7) AT4g05150/C17L7_70, partial (20%)
Length = 491
Score = 72.8 bits (177), Expect = 4e-14
Identities = 38/94 (40%), Positives = 61/94 (64%), Gaps = 1/94 (1%)
Frame = +1
Query: 3 RVKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFM-FSDVC 61
RV+ M+++ GKI PR HD+ + Y+GGD +I+ V+R+ +F L+ KLS + +
Sbjct: 208 RVRFMVSFRGKILPRP---HDN-QLRYVGGDTRIVAVNRSASFPALLLKLSKLSGMTKIT 375
Query: 62 FKYQLLGEDFDALIPIYNEEDLNHIMFEYDRMCH 95
KYQL E+ DALI + +ED+ +++ EYDR+ H
Sbjct: 376 LKYQLPNEELDALISVTTDEDVENMIDEYDRVAH 477
>AW719831
Length = 436
Score = 57.4 bits (137), Expect = 2e-09
Identities = 33/65 (50%), Positives = 42/65 (63%), Gaps = 1/65 (1%)
Frame = +3
Query: 3 RVKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFSDLMAKLSTFM-FSDVC 61
+VKLM +YGGKIQPR HD+ +Y+ GD KI+ VDR I +AKL++ SD C
Sbjct: 252 KVKLMCSYGGKIQPR---PHDN-NLTYVSGDTKILAVDRFIKLPAFIAKLTSLTNSSDFC 419
Query: 62 FKYQL 66
KYQL
Sbjct: 420 LKYQL 434
>TC10463 similar to UP|Q9LRV7 (Q9LRV7) Gb|AAF04880.1, partial (19%)
Length = 326
Score = 37.0 bits (84), Expect = 0.003
Identities = 17/43 (39%), Positives = 27/43 (62%)
Frame = +3
Query: 4 VKLMITYGGKIQPRLTALHDHLRYSYIGGDNKIITVDRNINFS 46
+K + +YGGKI PR + + Y+GG +++ VDR+I FS
Sbjct: 150 IKFLCSYGGKILPR----YPDGKLRYLGGHTRVLAVDRSIPFS 266
>BU494127
Length = 362
Score = 34.3 bits (77), Expect = 0.018
Identities = 21/59 (35%), Positives = 35/59 (58%), Gaps = 1/59 (1%)
Frame = +2
Query: 58 SDVCFKYQLLGED-FDALIPIYNEEDLNHIMFEYDRMCHFSRKPAWLRVFLFPVPINNN 115
+D+ FKYQL G + + L H+M EYDR+ S +P+ +R+FLF +P+ ++
Sbjct: 53 ADLTFKYQLPGRGPRRSHLRSPTTMILYHMMHEYDRLYRASARPSRMRLFLF-LPVESS 226
>TC18336 similar to UP|Q84KA9 (Q84KA9) RING/C3HC4/PHD zinc finger-like
protein, partial (15%)
Length = 761
Score = 29.3 bits (64), Expect = 0.56
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = -1
Query: 136 FEEYSSPPTTPPPPFMATKDTV 157
F +S+P TPPPPF ATK+ V
Sbjct: 503 FTHFSTP--TPPPPFFATKNDV 444
>BP053180
Length = 497
Score = 29.3 bits (64), Expect = 0.56
Identities = 13/31 (41%), Positives = 18/31 (57%)
Frame = -3
Query: 117 ASFDSLASVKSLNFVRVSPFEEYSSPPTTPP 147
AS ++ S+ SL F+ +SPFE T PP
Sbjct: 204 ASLPNMPSLNSLTFLEISPFEAELFTSTVPP 112
>TC18688 weakly similar to UP|Q9LP77 (Q9LP77) T1N15.9, partial (13%)
Length = 514
Score = 28.9 bits (63), Expect = 0.74
Identities = 20/45 (44%), Positives = 23/45 (50%)
Frame = +3
Query: 109 PVPINNNKASFDSLASVKSLNFVRVSPFEEYSSPPTTPPPPFMAT 153
P P N++ AS S S S V VS SPPT+PP P AT
Sbjct: 282 PSPANSHMAS--SPPSPTSALSVSVSTPSPAHSPPTSPPAPRSAT 410
>TC18261 similar to UP|WSC2_YEAST (P53832) Cell wall integrity and stress
response component 2 precursor, partial (5%)
Length = 538
Score = 28.9 bits (63), Expect = 0.74
Identities = 14/42 (33%), Positives = 23/42 (54%)
Frame = +3
Query: 108 FPVPINNNKASFDSLASVKSLNFVRVSPFEEYSSPPTTPPPP 149
F + +N+ + S A+ +S N + +P SPPT+PP P
Sbjct: 96 FSSALRSNRGNSRSFANKRSANS-KPNPLNAAQSPPTSPPQP 218
>AV409705
Length = 263
Score = 28.1 bits (61), Expect = 1.3
Identities = 17/40 (42%), Positives = 23/40 (57%), Gaps = 2/40 (5%)
Frame = +1
Query: 112 INNNKASFDSLASVKSLNF--VRVSPFEEYSSPPTTPPPP 149
I+ N A+ +L S S F V ++PFE +SSP TP P
Sbjct: 1 IHLNDATSQTLPSPHSSPFLPVIIAPFESFSSPSPTPHLP 120
>TC15921 weakly similar to GB|AAB64701.1|603415|SCE9163 Yer174cp
{Saccharomyces cerevisiae;} , partial (10%)
Length = 580
Score = 28.1 bits (61), Expect = 1.3
Identities = 24/79 (30%), Positives = 32/79 (40%), Gaps = 8/79 (10%)
Frame = -3
Query: 77 IYNEEDLNHIMFEYDRMCHFSRKPAWLRVFLFP----VPINNNKASFDSLASVKSLNFVR 132
IYN++ N I +Y R C ++ FP P KA S + LN +
Sbjct: 560 IYNQQFSNMIKIKYTRQCSSAKHE*LCTTINFPGSTSSPHLKKKA*MQSFSYF--LNTLA 387
Query: 133 VSPFE----EYSSPPTTPP 147
SPF S PPT+ P
Sbjct: 386 TSPFSYMEVNISQPPTSSP 330
>AU251432
Length = 364
Score = 27.7 bits (60), Expect = 1.6
Identities = 26/91 (28%), Positives = 39/91 (42%)
Frame = +2
Query: 122 LASVKSLNFVRVSPFEEYSSPPTTPPPPFMATKDTVTKAEMKKFRGLHVVNDEFEDILKL 181
L V S + R+S S PP PPPP + K + G V+ E D L+L
Sbjct: 32 LIGVTSFSPTRISRNSSSS*PPKNPPPPSSSLK----RRRSDNDDGAGEVSKEVNDFLRL 199
Query: 182 YLTCNFSKVKDFHGLHVVNDEFEDILKSFWK 212
+++ F+ V +E E ++K WK
Sbjct: 200 LEV----EIEKFNAFFVEMEE-EYVIK--WK 271
>CN824958
Length = 593
Score = 27.3 bits (59), Expect = 2.1
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = -3
Query: 99 KPAWLRVFLFPVP 111
KP W+R+FLFP P
Sbjct: 165 KPPWIRIFLFPNP 127
>BP053199
Length = 532
Score = 27.3 bits (59), Expect = 2.1
Identities = 23/59 (38%), Positives = 29/59 (48%), Gaps = 8/59 (13%)
Frame = +2
Query: 103 LRVFLFPVP----INNNKASFDSLASVKSLNFVRVSPFEEYSSP-PTT---PPPPFMAT 153
L + L P P + N K S + +SLN + VS S+P PTT PPPP AT
Sbjct: 134 LFITLSPSPAL*VVRNPKCSEQEVPQTESLNSINVS-----SAPSPTTASPPPPPSSAT 295
>TC14475
Length = 634
Score = 27.3 bits (59), Expect = 2.1
Identities = 15/30 (50%), Positives = 19/30 (63%)
Frame = -3
Query: 85 HIMFEYDRMCHFSRKPAWLRVFLFPVPINN 114
H++F R C S KP + VFLFP+ INN
Sbjct: 335 HLLFISVR*CPSSTKPDF--VFLFPI*INN 252
>TC19458 weakly similar to UP|Q9STE8 (Q9STE8) Chloroplast import-associated
channel homolog (AT3g46740/T6H20_230), partial (10%)
Length = 469
Score = 24.6 bits (52), Expect(2) = 2.5
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = -1
Query: 123 ASVKSLNFVRVSPFEEYSSPPTTPPPP 149
A V+SL+ + PF PP PPPP
Sbjct: 325 AGVRSLD--QKPPFPSPPPPPNQPPPP 251
Score = 20.8 bits (42), Expect(2) = 2.5
Identities = 7/24 (29%), Positives = 15/24 (62%)
Frame = -3
Query: 75 IPIYNEEDLNHIMFEYDRMCHFSR 98
+PI E+ +H++FE ++ +R
Sbjct: 461 VPIEEEDLRDHVLFEAAQLVQLNR 390
>AV424458
Length = 326
Score = 26.9 bits (58), Expect = 2.8
Identities = 17/44 (38%), Positives = 21/44 (47%), Gaps = 6/44 (13%)
Frame = +2
Query: 113 NNNKASFDSLA------SVKSLNFVRVSPFEEYSSPPTTPPPPF 150
NNN A+ SL + + L+F P S PP PPPPF
Sbjct: 89 NNNTATIQSLFFAILYYAAQPLSFSL--PITPSSPPPQPPPPPF 214
>TC19039 similar to UP|Q8L7W5 (Q8L7W5) AT4g12110/F16J13_180, partial (61%)
Length = 763
Score = 26.9 bits (58), Expect = 2.8
Identities = 16/62 (25%), Positives = 30/62 (47%)
Frame = +1
Query: 108 FPVPINNNKASFDSLASVKSLNFVRVSPFEEYSSPPTTPPPPFMATKDTVTKAEMKKFRG 167
FP I++ + F L+ + +LN + +PP +PPPP + ++ A M +
Sbjct: 25 FPNQISDPHSLFPFLSLLSTLNHL---------TPPPSPPPPALLSR---VPARMLPYHS 168
Query: 168 LH 169
+H
Sbjct: 169 IH 174
>AU089438
Length = 569
Score = 26.9 bits (58), Expect = 2.8
Identities = 20/58 (34%), Positives = 30/58 (51%), Gaps = 9/58 (15%)
Frame = -3
Query: 94 CH---FSRKPAWLRV-----FLFPVPINNNKASFDSLASVKSLNFVRV-SPFEEYSSP 142
CH F+ P +LRV FLFP+P ++ A DS +V F+ + SP ++ P
Sbjct: 228 CHHSLFAVLPPFLRVKHDHHFLFPIPAPSSVARSDSGVAVAQSRFLLLPSPRSDFFLP 55
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.325 0.141 0.433
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,415,941
Number of Sequences: 28460
Number of extensions: 68753
Number of successful extensions: 1473
Number of sequences better than 10.0: 71
Number of HSP's better than 10.0 without gapping: 1177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1391
length of query: 222
length of database: 4,897,600
effective HSP length: 87
effective length of query: 135
effective length of database: 2,421,580
effective search space: 326913300
effective search space used: 326913300
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 53 (25.0 bits)
Medicago: description of AC124972.5